1. Despite the array of sophisticated imaging techniques available for biological applications, none of the standard biomedical techniques adequately provides the capability to measure motion and flow. Those techniques currently in use are particularly lacking in spatial and temporal resolution. 2. Herein, we introduce the technique of particle image velocimetry. This technique is a well-established tool in engineering research and industry. Particle image velocimetry is continuing to develop and has an increasing number of variants. 3. Three case studies are presented: (i) the use of microparticle image velocimetry to study flow generated by high-frequency oscillatory ventilation in a human airway model; (ii) the use of stereoparticle image velocimetry to study stirred cell and tissue culture devices; and (iii) a three-dimensional X-ray particle image velocimetry technique used to measure flow in an in vitro vascular flow model. 4. The case studies highlight the vast potential of applying the engineering technique of particle image velocimetry and its many variants to current research problems in physiology.
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