A potential application of C60 for three-dimensional optical data storage in read only memory recorded by photo-oxidation is investigated. Photo-oxidation significantly enhances the fluorescence intensity of C60 molecules; therefore, one bit data can be recorded by the photo-oxidation of C60 doped polystyrene film at individual points by a focused laser beam and be read nondestructively as a brilliant fluorescence spot, in contrast to the nonoxidized areas after photo-oxidation is blocked. This enables three-dimensional recording using a laser scanning microscope. As a result, a pattern consisting of three layers of bit data was written and read in a C60 doped polystyrene film. We also discuss the time course of photo-oxidation of C60.