Landfilling is one of the important methods in hazardous waste treatment. In order to prevent the landfill leachate from connecting with external groundwater and prevent leachate from flowing into external polluted water bodies and soil, vertical anti-seepage membranes will be installed along the perimeter of the reservoir area. Given the structural characteristics of vertical HDPE membranes and site conditions, this study proposes the application of three-dimensional high-density electrical resistivity (3D-HDER) tomography for the detection of these membranes. Compared with the traditional resistivity method, the high-density resistivity method can realize automatic switching of electrode device type, electrode spacing and measuring points during the measurement process, thereby improving detection accuracy and work efficiency. By employing 3D-HDER for data collection on vertical HDPE membranes and inverting the subsurface resistivity distribution, the presence of leaks can be inferred based on the distribution of low-resistivity anomalies in the target underground area, thereby facilitating the integrity assessment of the vertical HDPE membranes. This research was applied in a practical investigation at a municipal landfill site in Hunan Province, where field tests were conducted, and the effectiveness of 3D-HDER in detecting vertical HDPE membranes was validated through excavation. The experimental results indicate that: (1) 3DHDER is capable of effectively and accurately locating shallow leaks, and (2) low resistivity anomalies were observed in the vicinity of the leaks.