Defensive Behavior Research Articles

Effects of Repetitive Mild Traumatic Brain Injury on Corticotropin-Releasing Factor Modulation of Lateral Habenula Excitability and Motivated Behavior.

Mild traumatic brain injury (mTBI) is a significant health burden due to mTBI-related chronic debilitating cognitive and psychiatric morbidities. Recent evidence from our laboratory suggests a possible dysregulation within reward/motivational circuit function at the level of a subcortical structure, the lateral habenula (LHb), where we demonstrated a causal role for hyperactive LHb in mTBI-induced motivational deficits in self-care grooming behavior in young adult male mice when exposed to mTBI during late adolescence (at ∼8 weeks old). In this study, we extended this observation by further characterizing neurobehavioral effects of this repetitive closed head injury model of mTBI in both young adult male and female mice on LHb excitability, corticotropin releasing factor (CRF) modulation of LHb activity, and behavioral responses of motivation to self-care behavior and approach versus avoidance behavior in the presence of a social- or threat-related stimulus. We show that mTBI increases LHb spontaneous tonic activity in female mice similar to what we previously observed in male mice, as well as promoting LHb neuronal hyperexcitability and hyperpolarization-induced LHb bursting in both male and female mice. Interestingly, mTBI only increases LHb intrinsic excitability in male mice coincident with higher levels of the hyperpolarization-activated cation currents (HCN/Ih) and reduces levels of the M-type potassium currents while potentiating M-currents without altering intrinsic excitability in LHb neurons of female mice. Because persistent dysregulation of brain CRF systems is suggested to contribute to chronic psychiatric morbidities and that LHb neurons are highly responsive to CRF, we tested whether the LHb CRF subsystem becomes engaged following mTBI. We found that in vitro inhibition of CRF receptor type 1 (CRFR1) within the LHb reverses mTBI-induced enhancement of LHb tonic activity and hyperexcitability in both sexes, suggesting that an augmented intra-LHb CRF-CRFR1-mediated signaling contributes to the overall LHb hyperactivity following mTBI. Behaviorally, mTBI diminishes motivation for self-care grooming in female mice as in male mice. mTBI also alters defensive behaviors in the looming shadow task by shifting the innate defensive behaviors toward more passive action locking rather than escape behaviors in response to an aerial threat in both male and female mice, as well as prolonging the latency to escape responses in female mice. While this model of mTBI reduces social preference in male mice, it induces higher social novelty seeking during the novel social encounters in both male and female mice. Overall, our study provides further translational validity for the use of this pre-clinical model of mTBI for investigation of mTBI-related reward circuit dysfunction and mood/motivation-related behavioral deficits in both sexes while uncovering a few sexually dimorphic neurobehavioral effects of this model that may differentially affect young males and females when exposed to this type of mTBI during late adolescence.

Hive Defensive Behavior in Japanese Honey Bees: The Bees Slap Ants with Their Wings to Prevent Intrusion

Hive Defensive Behavior in Japanese Honey Bees: The Bees Slap Ants with Their Wings to Prevent Intrusion

Cross-modal enhancement of defensive behavior via parabigemino-collicular projections

Effective detection and avoidance from environmental threats are crucial for animals' survival. Integration of sensory cues associated with threats across different modalities can significantly enhance animals' detection and behavioral responses. However, the neural circuit-level mechanisms underlying the modulation of defensive behavior or fear response under simultaneous multimodal sensory inputs remain poorly understood. Here, we report in mice that bimodal looming stimuli combining coherent visual and auditory signals elicit more robust defensive/fear reactions than unimodal stimuli. These include intensified escape and prolonged hiding, suggesting a heightened defensive/fear state. These various responses depend on the activity of the superior colliculus (SC), while its downstream nucleus, the parabigeminal nucleus (PBG), predominantly influences the duration of hiding behavior. PBG temporally integrates visual and auditory signals and enhances the salience of threat signals by amplifying SC sensory responses through its feedback projection to the visual layer of the SC. Our results suggest an evolutionarily conserved pathway in defense circuits for multisensory integration and cross-modality enhancement.

Performance Evaluation of Local Honey Bee Races (<i>Apis mellifera bandansi</i>) at Wondo Genet, Sidama Regional State of Southern Ethiopia

The research was undertaken in Wondo genet agricultural a study conducted at a research facility between 2015 and 2017 focused on assessing the performance of <i>Apis mellifera bandansi</i> honey bee races. The objective was to identify and choose the most effective honey bee races based on various parameters such as defensive behavior, hygienic practices, absconding tendencies, foraging abilities, swarming patterns, and productivity in terms of hive product area. A total of thirty bee races were evaluated during the study period (30) colonies of <i>Apis mellifera bandansi</i>i honeybee were established on station. Basic management practices were made reasonably uniform for tested colonies. The investigation indicated the races exhibited aggressive behavior, resulting in stings on the examiner, with a maximum count of up to 158±15.3 and a minimum of 60 stings on the manipulator. The distance covered by the races extended long, reaching up to 255±74.1 meters away from the entrance. The absconding percentage from a total of thirty colonies ranged from a maximum of 50% in 2015 to a minimum of 26% in 2017. The early and late foraging behavior of the colony was also documented early morning 5:30 am time foraging, whereas late foraging time was at 7:15pm pm e back to their nest in the evening. The remaining hive products were quantified in a 5x5 cm2 comb area cell over the course of the production season, with measurements taken at 21-day intervals to assess the brood area. The values obtained, expressed as the mean ± standard deviation, indicated the range from maximum to minimum for the adult, brood, pollen, nectar, and honey area in cm<sup>2</sup>. 79.5±13.4 107.1±24.6, 74.41±31.3, 48.2±9.0 for year (2015), 74.5±6.5, 107.1±24.6, 48.5±16.9, 81.8±31.6 for (year, 2016) and 114.8±20.5, 96.2±17.4, 50.0±19.4 and 90.7±40.2 for (year, 2017 respectively. The Apis mellifera, typically referred to in a general sense bandansi the race demonstrates positive attributes in terms of hygienic behavior and is also efficient in maximizing honey production when subjected to appropriate management strategies. It is recommended to conduct additional research on the specification of geographical location in relation to the distribution of different races and their identification for the purpose of breed determination and enhancement. This will aid in the selection of colonies that are more suitable in terms of production efficiency, temperament, resistance to pests, parasites, and diseases, as well as foraging and absconding tendencies.

SIKAP BELA NEGARA SEBAGAI KONTROL DIRI DALAM MEMINIMALISIR PERILAKU KECURANGAN DIPANDANG DARI PERSPEKTIF FRAUD DIAMOND

This research aim to test and empirically demonstrate how pressure, opportunity, rationalization, and capacity can influence academic fraud behaviour and how national defense attitudes play a role in moderating such influence. This research is a quantitative study using up to 94 students from S1 Accounting student UPN “Veteran” East Java as samples. The research used SEM PLS with WarpPLS 7.0 software for data analysis. The finding of this research may be used as a resource for universities related to fraud in the university environment, specially in S1 Accounting students and can provide an overview of fraud related to occurring, so that the universities can take determined further action to deal with such matters. Research findings demonstrate that pressure and rationalization have a significant influence on fraud behavior and national defense attitudes can moderate the influence of opportunity against fraud behavior. Opportunity and capability have no significant influence on fraud behavior and national defense attitudes do not moderate the influence of pressure, racinoalization, and capability against fraud behavior.

Chemogenetic Excitation of Ventromedial Hypothalamic Steroidogenic Factor 1 (SF1) Neurons Increases Muscle Thermogenesis in Mice.

Allostatic adaptations to a perceived threat are crucial for survival and may tap into mechanisms serving the homeostatic control of energy balance. We previously established that exposure to predator odor (PO) in rats significantly increases skeletal muscle thermogenesis and energy expenditure (EE). Evidence highlights steroidogenic factor 1 (SF1) cells within the central and dorsomedial ventromedial hypothalamus (c/dmVMH) as a modulator of both energy homeostasis and defensive behavior. However, the brain mechanism driving elevated EE and muscle thermogenesis during PO exposure has yet to be elucidated. To assess the ability of SF1 neurons of the c/dmVMH to induce muscle thermogenesis, we used the combined technology of chemogenetics, transgenic mice, temperature transponders, and indirect calorimetry. Here, we evaluate EE and muscle thermogenesis in SF1-Cre mice exposed to PO (ferret odor) compared to transgenic and viral controls. We detected significant increases in muscle temperature, EE, and oxygen consumption following the chemogenetic stimulation of SF1 cells. However, there were no detectable changes in muscle temperature in response to PO in either the presence or absence of chemogenetic stimulation. While the specific role of the VMH SF1 cells in PO-induced thermogenesis remains uncertain, these data establish a supporting role for SF1 neurons in the induction of muscle thermogenesis and EE similar to what is seen after predator threats.

Role of L- and T-type voltage-dependent calcium channels in the hierarchical organization of defensive responses to electrical stimulation of the rat dorsolateral periaqueductal gray

Stimulation of the dorsal half of the rat periaqueductal gray (DPAG) with 60-Hz pulses of increasing intensity, 30-μA pulses of increasing frequency, or increasing doses of an excitatory amino acid elicits sequential defensive responses of exophthalmia, immobility, trotting, galloping, and jumping. These responses may be controlled by voltage-gated calcium channel-specific firing patterns. Indeed, a previous study showed that microinjection of the DPAG with 15 nmol of verapamil, a putative blocker of L-type calcium channels, attenuated all defensive responses to electrical stimulation at the same site as the injection. Accordingly, here we investigated the effects of microinjection of lower doses (0.7 and 7 nmol) of both verapamil and mibefradil, a preferential blocker of T-type calcium channels, on DPAG-evoked defensive behaviors of the male rat. Behaviors were recorded either 24 h before or 10 min, 24 h, and 48 h after microinjection. Effects were analyzed by both threshold logistic analysis and repeated measures analysis of variance for treatment by session interactions. Data showed that the electrodes were all located within the dorsolateral PAG. Compared to the effects of saline, verapamil significantly attenuated exophthalmia, immobility, and trotting. Mibefradil significantly attenuated exophthalmia and marginally attenuated immobility while facilitating trotting. While galloping was not attenuated by either antagonist, jumping was unexpectedly attenuated by 0.7 nmol verapamil only. These results suggest that T-type calcium channels are involved in the low-threshold freezing responses of exophthalmia and immobility, whereas L-type calcium channels are involved in the trotting response that precedes the full-fledged escape responses of galloping and jumping.

Wing-slapping: A defensive behavior by honey bees against ants.

Wing-slapping: A defensive behavior by honey bees against ants.

A vagal-brainstem interoceptive circuit for cough-like defensive behaviors in mice.

Coughing is a respiratory behavior that plays a crucial role in protecting the respiratory system. Here we show that the nucleus of the solitary tract (NTS) in mice contains heterogenous neuronal populations that differentially control breathing. Within these subtypes, activation of tachykinin 1 (Tac1)-expressing neurons triggers specific respiratory behaviors that, as revealed by our detailed characterization, are cough-like behaviors. Chemogenetic silencing or genetic ablation of Tac1 neurons inhibits cough-like behaviors induced by tussive challenges. These Tac1 neurons receive synaptic inputs from the bronchopulmonary chemosensory and mechanosensory neurons in the vagal ganglion and coordinate medullary regions to control distinct aspects of cough-like defensive behaviors. We propose that these Tac1 neurons in the NTS are a key component of the airway-vagal-brain neural circuit that controls cough-like defensive behaviors in mice and that they coordinate the downstream modular circuits to elicit the sequential motor pattern of forceful expiratory responses.

The influence of individuality on performance traits in mussels: Assessments based on defensive behavior and physiology

Cultured Mytilus coruscus fall-offs regularly led to the reduction of harvest yields of suspension-cultured mussels. High temperature and marine pollutants affect mussel physiological health, which increases the risk of mussel fall-offs and results in yield declines. In this study, we classified mussels into proactive and reactive groups based on their self-organizing behavior after air exposure stress and investigated the differences in defense performance of different group mussels. Proactive mussels were found to have a significantly higher byssus number, adhesion area, and gene expression in the mussel foot protein mfp5 and the byssus collagen preCOL-P. Exposure of mussels to high temperature and different concentrations of BDE-47 revealed a decrease in byssus performance and byssus gene expression in both proactive and reactive groups, but the decreased level of expression of the proactive mussel foot proteins mfp5 and mfp6 was significantly less than that of the reactive mussels. Our study found that proactive mussels have more and better byssuses, better adhesion and defense ability, and greater adaptability under higher temperature and pollutant-exposed conditions. The performance of byssus is correlated with the individuality of stress coping, providing a new direction for future mussel selection breeding and aquaculture.

Defensive behavior: Sensing threats from terrestrial and aerial predators

The dorsal periaqueductal gray (dPAG) contains a tonically GABAergic network controlling defensive responses. Determining how this intrinsic dPAG inhibitory circuit functions might provide critical insights into how anti-predatory responses are organized.

Fear, defense, and emotion: A neuroethological understanding of the negative valence research domain criteria.

We describe the close correspondence between predatory imminence continuum theory (PICT) and the National Institute of Mental Health's Research Domain Criteria (RDoC) for negative valence. RDoC's negative valence constructs relate aversively motivated behavioral reactions to various levels of threat. PICT divides defensive responses into distinct modes that vary along a continuum of the psychological closeness of predatory threat. While there is a close correspondence between PICT modes and negative valence threat constructs, based on PICT, we describe some potential elaborations of RDoC constructs. Both have consonant views of fear and anxiety and provide explicit distinctions between these emotional states, relating them to specific defensive behaviors and functions. We describe recent data that causally implicate human subjective emotional states with amygdala activity, which is also critical for defensive behavior. We conclude that attention to neuroethological views of defense can advance our understanding of the etiology and treatment of anxiety and stress disorders. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Defensive plasticity in South American rattlesnakes Crotalus durissus

Reptiles exhibit defensive responses in diverse ways, influenced by situational factors. Snakes, such as the South American rattlesnake Crotalus durissus, display complex defensive responses, adapting tactics based on circumstances when encountering potential predators. Environmental and intrinsic parameters shape snake behaviour during predator encounters. This study employs distinct stimuli to investigate behavioural changes and whether snakes adapt defensive strategies. We compare the behaviours of rattlesnakes before and after an aversive stimulus involving physical contact. Following encounters with higher-risk stimuli, snakes become more defensive.Findings reveal that snakes alter strategies based on threat intensity. This phenomenon might be influenced by the potential cost of defense and prior interactions with predators. The study underscores the intricate nature of snake defensive behaviour. Keywords: behavioural plasticity, antipredator behaviour, aversive stimulus, behavioural cost, active behaviours

Modeling Host-Pathogen Interactions in C. elegans: Lessons Learned from Pseudomonas aeruginosa Infection.

Infections, such as that by the multiresistant opportunistic bacterial pathogen Pseudomonas aeruginosa, may pose a serious health risk, especially on vulnerable patient populations. The nematode Caenorhabditis elegans provides a simple organismal model to investigate both pathogenic mechanisms and the emerging role of innate immunity in host protection. Here, we review the virulence and infection strategies of P. aeruginosa and host defenses of C. elegans. We summarize the recognition mechanisms of patterns of pathogenesis, including novel pathogen-associated molecular patterns and surveillance immunity of translation, mitochondria, and lysosome-related organelles. We also review the regulation of antimicrobial and behavioral defenses by the worm's neuroendocrine system. We focus on how discoveries in this rich field align with well-characterized evolutionary conserved protective pathways, as well as on potential crossovers to human pathogenesis and innate immune responses.

Neural Decoding and Feature Selection Techniques for Closed-Loop Control of Defensive Behavior.

Many psychiatric disorders involve excessive avoidant or defensive behavior, such as avoidance in anxiety and trauma disorders or defensive rituals in obsessive-compulsive disorders. Developing algorithms to predict these behaviors from local field potentials (LFPs) could serve as foundational technology for closed-loop control of such disorders. A significant challenge is identifying the LFP features that encode these defensive behaviors. We analyzed LFP signals from the infralimbic cortex and basolateral amygdala of rats undergoing tone-shock conditioning and extinction, standard for investigating defensive behaviors. We utilized a comprehensive set of neuro-markers across spectral, temporal, and connectivity domains, employing SHapley Additive exPlanations for feature importance evaluation within Light Gradient-Boosting Machine models. Our goal was to decode three commonly studied avoidance/defensive behaviors: freezing, bar-press suppression, and motion (accelerometry), examining the impact of different features on decoding performance. Band power and band power ratio between channels emerged as optimal features across sessions. High-gamma (80-150 Hz) power, power ratios, and inter-regional correlations were more informative than other bands that are more classically linked to defensive behaviors. Focusing on highly informative features enhanced performance. Across 4 recording sessions with 16 subjects, we achieved an average coefficient of determination of 0.5357 and 0.3476, and Pearson correlation coefficients of 0.7579 and 0.6092 for accelerometry jerk and bar press rate, respectively. Utilizing only the most informative features revealed differential encoding between accelerometry and bar press rate, with the former primarily through local spectral power and the latter via inter-regional connectivity. Our methodology demonstrated remarkably low time complexity, requiring <110 ms for training and <1 ms for inference. Our results demonstrate the feasibility of accurately decoding defensive behaviors with minimal latency, using LFP features from neural circuits strongly linked to these behaviors. This methodology holds promise for real-time decoding to identify physiological targets in closed-loop psychiatric neuromodulation.

Interspecific variation in the defensive responses of brown frogs to alien predators

AbstractAlien species impact native amphibians through various direct effects, including predation, and pose a significant threat to naïve prey populations. In this study, we exposed tadpoles of three brown frog species (Rana dalmatina, Rana latastei, and Rana temporaria) to the olfactory cues of two alien predators, the pond slider (Trachemys scripta) and red swamp crayfish (Procambarus clarkii), and compared their responses to those induced by the aquatic larvae of a widespread native predator, the Southern hawker (Aeshna cyanea). We recorded two tadpole defensive behaviors, the proportion of time they were active and the number of freezing events. Both agile frog species, R. dalmatina and R. latastei, showed moderate responses to red swamp crayfish kairomones and strong responses to both odonate larvae and pond sliders. In contrast, the common frog (R. temporaria) displayed a less intense response to crayfish with respect to odonate larvae, and a negligible response to pond sliders. Long-lasting coexistence with either European pond turtles or pond sliders may explain the strength of agile frogs’ response toward the alien species; while, the historical range of the white-clawed crayfish (Austropotamobius pallipes) overlapped that of the common frog, enhancing the co-option of alien crayfish cues by this anuran species.

A Case Study on Seasonal Behaviour of Aphodius elegans Allibert an Endocoprid Dung Beetle

Dung beetles (Scarabaeidae) classified into endocoprid, paracoprid, and telecoprid beetles, are fascinating insects with various behaviors. The study focuses on behaviour of endocoprid beetles Aphodius elegans Allibert, found in Bumdeling, Trashi Yangts Bhutan. The observational and scan sampling methods were employed during the study. These beetles locate dung using antennae and dig inside it, resembling a boat propelled by oars. They are active during warm weather and bury themselves under dung during cold. Beetles defecate semi solid feces after dung foraging. They mimic death as defensive and antipredator behaviour. They prefer moist dung pats for burial and use repeated attempts and strongholds of hindwings to initiate flight. This study substantiates the diverse fascinating behaviors of a less-focused group of insects to gain a deeper understanding of their interactions with ecology.

Construction of Power System Network Security Defense Behavior Decision-making Model based on Artificial Intelligence Technology

According to the needs of power grid monitoring architecture and information security cooperation protection, this project builds a multi-level, deeply distributed active security cooperation defense mode. A complete implementation method is proposed from the perspective of model architecture and function mechanism. The optimal defense strategy based on grey correlation is studied according to the characteristics of cooperation between regions. In this way, the coordination between the equipment is realized to achieve multi-level protection from the host layer to the security equipment layer and then to the network layer. Multiple detection mechanisms are used to realize the comprehensive detection and integrated judgment of abnormal documents in the cloud environment. This provides maximum protection for cloud users. Experiments show that this method can effectively suppress the malicious attacks of malicious users and reduce the damage caused by viruses. In this way, both the cloud and the customer are protected.

Against network attacks in renewable power plants: Malicious behavior defense for federated learning

As reducing carbon emissions can relieve environmental concerns, networks-supported renewable power plants are being built more and more. Inevitable network attacks have become a serious threat in increasing and distributed power plants. Leveraging federated learning for training the joint model to detect network attacks in distributed power plants is efficient, but two malicious behaviors of cheating and free-riding are unavoidable. To this end, we design a new SDN based federated security architecture and propose a carbon-credit-rewarded consensus verification mechanism in this architecture to deal with malicious behaviors. For this architecture, on the one hand, considering geographical conditions of renewable power plants, multi-controller SDN is adopted in network to solve some security problems at root and to avoid single point of failure. On the other hand, the segmentation of collaborative zones reduces communication cost effectively. The proposed mechanism establishes consensus bearer and realizes the election of consensus bearer by cross-validation of client detection models. Only the excellent models are aggregated to mitigate cheating of malicious clients. Carbon emissions credit is introduced as an incentive in the proposed mechanism. The redistribution of carbon emissions credit improves the performance of global detection model and avoids free-riding. Moreover, the economic nature of carbon emissions credit enhances the spillover effect of carbon emissions trading market on the reduction of carbon emissions. The experimental results revealed that the proposed architecture has excellent performance, and can handle malicious behaviors effectively.

Comparing behaviours induced by natural memory retrieval and optogenetic reactivation of an engram ensemble in mice.

Memories are thought to be stored within sparse collections of neurons known as engram ensembles. Neurons active during a training episode are allocated to an engram ensemble ('engram neurons'). Memory retrieval is initiated by external sensory or internal cues present at the time of training reactivating engram neurons. Interestingly, optogenetic reactivation of engram ensemble neurons alone in the absence of external sensory cues is sufficient to induce behaviour consistent with memory retrieval in mice. However, there may exist differences between the behaviours induced by natural retrieval cues or artificial engram reactivation. Here, we compared two defensive behaviours (freezing and the syllable structure of ultrasonic vocalizations, USVs) induced by sensory cues present at training (natural memory retrieval) and optogenetic engram ensemble reactivation (artificial memory retrieval) in a threat conditioning paradigm in the same mice. During natural memory recall, we observed a strong positive correlation between freezing levels and distinct USV syllable features (characterized by an unsupervised algorithm, MUPET (Mouse Ultrasonic Profile ExTraction)). Moreover, we observed strikingly similar behavioural profiles in terms of freezing and USV characteristics between natural memory recall and artificial memory recall in the absence of sensory retrieval cues. Although our analysis focused on two behavioural measures of threat memory (freezing and USV characteristics), these results underscore the similarities between threat memory recall triggered naturally and through optogenetic reactivation of engram ensembles. This article is part of a discussion meeting issue 'Long-term potentiation: 50 years on'.