Objective To explore clinical value of the single-hole video-assisted thoracoscope surgery in lung surgery. Methods In our hospital from January 2015 to June 2015, a total of 60 patients was performed with lung partial resection line thoracoscope surgery, 28 routine puckering traditional video-assisted thoracoscope treatment, in the same period 32 underwent video-assisted thoracoscope surgery. Intraoperative blood loss, operating time, postoperative pain degree, and postoperative chest such as flow rate were compared between two groups. Results Two groups of patients had no perioperative death and severe complications. Intraoperative blood loss and operating time were no significant significance between single- and double-hole operation groups. The degree of postoperative pain score was (0.8±0.5) in puckering thoracoscope group, and (1.2±0.6) in traditional thoracoscope group, with significant difference (P=0.009). Conclusions Single-hole thoracoscope surgery in the treatment of lung diseases is feasible and reliable compared to traditional thoracoscope surgery, and patients can effectively reduce postoperative pain, and improve the quality of life. Key words: Thoracoscopy/MT; Pneumonectomy