The study was conducted in 25 black Bengal does to determine the exact site of infiltration of local anaesthetics for the last thoracic (T13), first lumbar (L1) and second lumbar (L2) paravertebral spinal nerve block at the Dept. of Anatomy and Histology, Chittagong Veterinary and Animal Sciences University, Chittagong. The animals were anaesthetized with pentobarbital sodium @ 20 mg / kg body weight IM and bled to death by giving incision on the right common carotid artery and preserved by 10 % formalin and dissected carefully to visualize the spinal nerves for the determination of the exact site of infiltration of local anaesthetic at the perineural area for paravertebral nerve block. For the vertical or proximal approach, the T13 spinal nerve was located at 0.8 ± 0.17 cm caudally from head of the last rib, 0.7 ± 0.22 cm cranio-medially from cranio-lateral angle of L1 transverse process and 3.4 ± 0.20 cm laterally from dorsal midline; L1 spinal nerve was placed at 1.2 ± 0.40 cm medially from cranio-lateral angle of L2 transverse process and 3.5 ± 0.17 cm laterally from the dorsal midline; and L2 spinal nerve was situated at 1.6 ± 0.26 cm medially from cranio-lateral angle of L3 transverse process and 3.7 ± 0.14 cm laterally from the dorsal midline. In horizontal or distal approach, T13 spinal nerve was located at 0.5 ± 0.10 cm and 1.8 ± 0.63 cm cranially from the cranio-lateral and caudo-lateral angles of L1 transverse process respectively and 4.6 ± 0.30 cm laterally from dorsal midline at lateral border of L1 transverse process; L1 spinal nerve was placed at 0.4 ± 0.10 cm and 1.4 ± 0.28 cm caudally and cranially from the cranio-lateral and caudo-lateral angle of L2 transverse process respectively and 5.1 ± 0.20 cm laterally from dorsal midline at lateral border of L2 transverse process and L2 spinal nerve was situated at 2.5 ± 0.42 cm and 0.5 ± 0.10 cm caudally and cranially from the cranio-lateral and caudo-lateral angle of L3 transverse process respectively and 5.5 ± 0.28 cm laterally from dorsal midline at lateral border of L3 transverse process. These measurements will be helpful to determine of the exact site for the deposition of the local anaesthetic agent at the perineural area of the specific paravertebral spinal nerve during paravertebral regional anaesthesia in doe. DOI = 10.3329/bjvm.v8i1.8354 Bangl. J. Vet. Med. (2010). 8(1): 81-86