Thiol Peroxidase Research Articles

Thiol peroxidase activity of diaryl ditellurides as determined by a proton NMR method

A 1 H NMR method was developed for the assessment of the gluthathione peroxidase-like activity of synthetic compounds. In this assay, thiols (N-acetylcysteine, tert-butyl mercaptan and 1-octyl mercaptan) were oxidized to the corresponding disulfides in CD 3 OD or CD 3 OD/D 2 O in the presence of hydrogen peroxide and the catalyst to be evaluated. The time required to reduce the thiol concentration with 50%, t 50 , was determined as a measure of the thiol peroxidase activity of the catalyst