Thiocyanate Solutions Research Articles

Hydrogen Embrittlement Detection Technology Using Nondestructive Testing for Realizing a Hydrogen Society.

Crack detection in high-pressure hydrogen gas components, such as pipes, is crucial for ensuring the safety and reliability of hydrogen infrastructure. This study conducts the nondestructive testing of crack propagation in steel piping under cyclic compressive loads in the presence of hydrogen in the material. The specimens were hydrogen-precharged through immersion in a 20 mass% ammonium thiocyanate solution at 40 °C for 72 h. The crack growth rate in hydrogen-precharged specimens was approximately 10 times faster than that in uncharged specimens, with cracks propagating from the inner to outer surfaces of the pipe. The fracture surface morphology differed significantly, with flat surfaces in hydrogen-precharged materials and convex or concave surfaces in uncharged materials. Eddy current and hammering tests revealed differences in the presence of large cracks between the two materials. By contrast, hammering tests revealed differences in the presence of a half size crack between the two materials. These findings highlight the effect of hydrogen precharging on crack propagation in steel piping and underscore the importance of early detection methods.

Optimized quantification of fatty acids in complex media using advanced analytical techniques.

Various medium formulations contain essential fatty acids at concentrations ranging from 10 to 100 mg/L. Accurate and precise lipid measurement in media is crucial for monitoring media quality and conducting studies on lipids in the context of cell culture. This study employed two-dimensional gas chromatography (GC × GC) analyses to offer enhanced resolution, sensitivity, and separation performance compared to GC. Quantification of fatty acid methyl esters (FAMEs) in a medium was conducted using GC × GC combined with a high-resolution mass spectrometer and flame ionization detector, considering potential interference from nonionic surfactant Tween 80, which was precipitated and removed by optimizing the concentration of cobalt thiocyanate (CTA) solution during pretreatment. This advanced analytical approach enabled identification of cis and trans isomers of identical molecular weights and determination of the location and number of double bonds in the same carbon number structure. Our analysis identified 36 FAMEs within the C6-C24 region, and a 5% CTA solution was optimal for efficient removal of Tween 80 during lipid extraction. Additionally, this advanced method minimized FAME contamination and loss during pretreatment, thereby significantly reducing the sample volume required to detect trace levels of FAMEs. This improvement led to a fatty acid recovery rate of 106% while maintaining the average relative standard deviation for the target FAMEs of about 3%. Our research paves the way for future investigation into medium quality control and the role of fatty acids in cell culture. This offers the possibility for economical and effective trace quantification of fatty acids in complex media.

Hydrogen-assisted cracking and damage tolerance of high-strength lath-martensite steel bars for structural applications

The paper addresses the hydrogen-assisted cracking and damage tolerance of high-strength lath martensite steel bars assessed from slow strain rate tensile tests. These were carried out on fatigue precracked specimens simultaneously charged with hydrogen in a standardized 20 % aqueous solution of ammonium thiocyanate at 50 °C and in air, at room temperature, for comparison. The fractographic analysis of the tested specimens showed the steel sensitivity to hydrogen uptake, which gives rise to the sub-critical extension of the pre-existing crack in two phases, initiation and growth, with both hydrogen-enhanced decohesion (HEDE) and hydrogen-enhanced localized plasticity (HELP) being synergically involved as damage mechanisms. Despite this, the damage tolerance behavior of the steel is highly satisfactory because the crack size causing the steel failure in tension slightly exceeds the theoretical limit of plastic collapse under combined tension and bending loading, regardless of the environment aggressiveness. Although H-assisted failure loads fit the theoretical limit loads, the fast propagation of the crack under constant load beyond the small-scale yielding regime could represent a real threat for the structural use of these steel bars in aggressive media unless the service loads are limited in accordance with such behavior.

Novel biologically active paddlewheel-like palladium thiocyanate complex catalyzed cyanation of alkyl bromide utilizing Q-tube system: A green perspective

A novel paddlewheel-like complex of [Pd(SCN)2(3-Acpy)2], (1) was produced at ambient temperature by adding an aqueous solution of palladium chloride to an aqueous acetonitrile solution of 3-acetylpyridine (3-Acpy) and potassium thiocyanate. Elemental analysis, Fourier-transform infrared (FT-IR), ultraviolet–visible (UV–vis), and nuclear magnetic resonance (NMR) spectroscopic methods were used to characterize complex 1 comprehensively. Single crystal X-ray diffraction was used to analyze the crystal structure of complex 1. The palladium atom is bonded to two 3-Acpy ligands and two thiocyanates groups in trans mode giving square planer geometry in complex 1. The discrete units of paddlewheel-like complex 1 through hydrogen bonding extend to form a 3D supramolecular network structure. The synthesized and characterized new palladium complex 1 was utilized as a precatalyst for the cyanation of alkyl bromide using potassium ferrocyanide as a benign cyanide source reagent. The suggested catalytic test reaction under high-pressure conditions via a Q-tube system in green solvent cyclopentyl methyl ether introduced high yields and a short reaction time. Moreover, the cytotoxic activity of complex 1 was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against HePG-2 of liver cancer and MCF7 of breast cancer cell lines. According to the findings, complex 1 has significant strong activity against the cancer cell lines HePG-2 and MCF-7. Additionally, the luminescence spectra of complex 1 were investigated.

Heap sulphuric-thiocyanate leaching of gold and uranium

The article evaluates the main parameters of simultaneous heap leaching of gold and uranium from oxidised gold-uranium ore using sulphuric acid thiocyanate solutions. Pilot tests for the simultaneous heap leaching of gold and uranium were carried out using oxidised crushed gold-uranium ore with a size of -40+0 mm. The gold and uranium content in the ore was 0.80 and 266 g/t, respectively. Experiments were carried out using a percolation column with a diameter of 300 mm and a height of 2000 mm The ore mass in the column was 180 kg. The temperature during the tests was in the range of 17–25°C. Leaching was carried out under the following conditions: H2SO4 concentration – 5 g/dm3, SCN– concentration – 0.5 g/dm3, Eh – 490–510 mV, Fe3+ ion concentration – 1.0–1.5 g/dm3. Acid-soluble minerals contained in the ore comprised the source of iron ions. Hydrogen peroxide was used to oxidise Fe2+ ions. Pilot tests were carried out in a closed cycle with separate sorption of gold and uranium. Based on the research results, gold recovery reached 90%, while uranium recovery was 55%. Following gold and uranium leaching, the loaded activated carbons and ion exchange resins were obtained. It was established that the gold content on activated carbons was 0.5–0.6 mg/g, while the uranium content on ion exchange resins amounted to 30–35 mg/g. The reagent consumption was as follows: H2SO4 – 10.5 kg/t, KSCN – 0.94 kg/t, H2O2 – 0.65 kg/t. It is shown that the technology for simultaneous heap leaching of gold and uranium with sulphuric acid thiocyanate solutions offers efficient processing of the ore. The recovery rates of gold and uranium are comparable to those obtained during pilot tests for heap leaching of the ore using conventional technology, involving the individual twostage leaching of these metals using sulphuric acid and cyanide solutions.

Extraction/transportation of Co2+ metal ions with bis(2-ethylhexyl) phosphate dissolved in kerosene using a multidropped liquid membrane technique.

The transport properties of Co2+ ions from the aqueous donor phase to aqueous acceptor phase with the recently developed multidroplet liquid membrane (MDLM) extraction system were studied. This system serves as a continuous process for the transportation of ions and requires fewer reagents for starting and conducting the procedure. Moreover, the procedure results in fewer waste chemicals and mixtures in comparison to traditional extraction methods. During extraction, bis(2-ethylhexyl) phosphate (D2EHPA) was used as a carrier material and 5% potassium thiocyanate (KSCN) solution was used to obtain a colored complex for UV-Vis detection. By means of several experiments, the optimum D2EHPA concentration, pH range for both donor and acceptor phases, and temperature range effect on transport kinetics were investigated. In the extraction of cobalt ions with the MDLM system, the activation energy was calculated as Ea = 13.80 kcal mol-1, and it was found that the extraction was chemically controlled since it was greater than 10 kcal mol-1.

Sustainable and effective gold(I) separation from aurocyanides wastewater and its mechanism using guanidinium ionic liquids

Efficient recovery of gold(I) from aurocyanide wastewater offers a dual opportunity for secondary resource utilization and environmental harm reduction. Herein, four environmentally friendly hexaalkylguanidium ionic liquids (HGILs) were synthesized and for the first time used to extract gold(I) from aurocyanide wastewater. Critical parameters, including HGILs and modifier species, dosage, pH, initial gold(I) concentration, and temperature, were optimized. The HGIL systems exhibited excellent gold(I) extraction performance with over 99.0% of gold(I) being extracted under optimal conditions. The composition ratio of the extracted complex was determined by Job's method and slope analysis. Spectral characterization included X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy and density functional theory calculations confirmed that gold(I) extraction followed an anion exchange mechanism. A dicyanoaurate Au(CN)2− anion and two n-octanol molecules produced the supermolecule anion [Au(CN)2−·2 n-octanol] via hydrogen bonding, which combined with a guanidium cation to generate the stable extraction complex [HGIL+·Au(CN)2−·2 n-octanol] through electrostatic attraction. The gold(I) loaded in the ionic liquid phase was effectively stripped with potassium thiocyanate solution. The regenerated HGIL system maintained a high gold(I) extraction efficiency of over 90.0% after five extraction–stripping cycles. In addition, a two-step stripping strategy was developed for the selective separation of gold(I) and other impurity metal ions from real aurocyanide wastewater. This study proposes an efficient green process for recovering gold(I) from mine wastewater, which has potential practical application prospects.

Detection of thiocyanate in postmortem blood of a fatal potassium thiocyanate-poisoning patient using gas chromatography-mass spectrometry.

Thiocyanate is an inorganic compound used in industrial applications. Here, we report a case of suicidal death due to acute thiocyanate overdose. A 44-year-old man who consumed an unknown amount of thiocyanate solution was transferred to the emergency room and died two hours after admission. An autopsy was performed two days after death. General toxicological analysis of blood using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-tandem mass spectrometry found no drug or alcohol. Quantification using GC-MS post-derivatization with pentafluorobenzyl bromide revealed 2,290 and 1,920 mg/L of thiocyanate in the heart and femoral blood samples, respectively. Thus, the cause of death was attributed to thiocyanate overdose. This study provides useful information for the interpretation of thiocyanate-related fatalities.

A mononuclear N,N,N,O donor schiff base Cu(II) complex inhibits bacterial biofilm formation and promotes apoptosis and cell cycle arrest in prostate cancer cells

In this work, we report a distorted square pyramidal mononuclear copper(II) complex [Cu(L)(NCS)] (1) which was obtained by the reaction of the aqueous solution of ammonium thiocyanate to a methanolic solution of copper nitrate trihydrate and corresponding Schiff-base ligands. Schiff bases, HL (C12H19N3O) act as a tetradentate Schiff base, derived from 1:1 condensation of o-hydroxyacetophenone and diethylenetriamine. The synthesized complex has been successfully characterized based on elemental analysis and Infrared (IR) spectroscopy. The structure of complex 1 was confirmed by single-crystal X-ray diffraction study. In our study, we investigated synthesis, structural characterization, antimicrobial, anti-biofilm, and anti-cancer activity, and plausible mechanism of action of a novel mononuclear copper(II) schiff base complex. Increasing microbial resistance to several commercially available or traditional antimicrobial compounds has become a major global health concern at present time. The mononuclear copper(II) complex exhibited potential antibacterial activity against two strains of the gram-negative pathogen Pseudomonas aeruginosa. The copper compound dependent damage of bacterial cell membrane and inhibition of bacterial biofilm formation were also identified. Moreover, complex 1 inhibited prostate cancer cell growth, and migration by inducing apoptosis and arresting the cell cycle at the G2/M phase. Based on the results, we are suggesting our novel mononuclear copper(II) compound as a potential candidate for the development of new antibacterial and anti-cancer drugs.

A Refractive Index- and Density-Matched Liquid–Liquid System Developed Using a Novel Design of Experiments

Refractive index and density matching are essential for optical measurements of neutrally buoyant liquid–liquid flows. In this study, we proposed a design of experiments (DoE) to develop refractive index and density matching systems, including objective setting, candidates screening, sampling and fitting, and a detailed matching process. Candidates screening criteria based on the density and refractive index ranges of the aqueous and organic phases were used. Using the DoE, we proposed a system with a ternary aqueous phase potassium thiocyanate (KSCN)/ammonium thiocyanate (NH4SCN) solution and m-dichlorobenzene/tripropionin solution as the organic phase to achieve the tuning of the RI and density simultaneously. Empirical correlations of the refractive index and density with respect to the concentration and temperature for the three mixtures were obtained by combining Latin hypercube sampling with binary polynomial fitting. Correlations were validated with existing data in the literature and were found to align with deviations as low as 4×10−4 for the refractive index and 2×10−3 g⋅cm−3 for the density. Using the correlations, the refractive indices for the ternary aqueous phase, the binary organic phase, and the device materials were matched to be equal. Density matching was performed for the liquid–liquid phases as well. Refractive index- and density-matched recipes could be obtained for a wide range of temperatures (15–65 °C) and device materials (PMMA, borosilicate glass, quartz, and silica gel). These recipes provide options for the optical measurement of a liquid–liquid system required to neutralize buoyancy.

Synthesis, structure characterization and antitumor activities of copper and cobalt thiocyanate complexes with 3-acetlpyridine ligand

Complexes of [Cu(SCN)2(3-Acpy)2], (1) and [Co(SCN)2(H2O)2(3-Acpy)2], (2) were synthesized at ambient temperature by adding an aqueous solution of copper(II) or cobalt(II) chloride to H2O/CH3CN solution of 3-acetlpyridine (3-Acpy) and potassium thiocyanate. Elemental analysis, FT-IR and UV–vis spectra were used to comprehensively characterize complexes 1 and 2. Single crystal X-ray diffraction was used to analyze the crystal structure of complexes 1 and 2. In complex 1, the copper is bonded to two 3-Acpy ligands and two thiocyanates groups in trans mode giving square planer geometry. While, in complex 2, the cobalt ion is coordinated to two water molecules, two 3-Acpy ligands and two thiocyanates ions in trans mode giving an octahedral structure. Through hydrogen bonding, the discrete units of complexes 1 and 2 extend to form a 3D supramolecular network. Additionally, the luminescence spectra of complexes 1 and 2 were investigated. Complexes 1 and 2 were evaluated to determine whether they were able to inhibit the growth of liver cancer (HepG2 cells) and breast cancer (MCF-7 cells). Complex 1 exhibits lower IC50 values than complex 2 against both of the two human cell lines. In HepG2 cells treated with complexes 1 and 2 at dosages roughly half of the IC50, apoptosis of complexes 1 and 2 is triggered by 18% and 22%, respectively. It was found that copper complex 1 has a greater cytotoxic effect on MCF-7 and influences on late apoptosis of HepG2.

Pseudohalogen Resurfaced CsPbBr3 Nanocrystals for Bright, Efficient, and Stable Green-Light-Emitting Diodes

Lead halide perovskite nanocrystals (LHP NCs) are regarded as promising emitters for next-generation ultrahigh-definition displays due to their high color purity and wide color gamut. Recently, the external quantum efficiency (EQE) of LHP NC based light-emitting diodes (PNC LEDs) has been rapidly improved to a level required by practical applications. However, the poor operational stability of the device, caused by halide ion migration at the grain boundary of LHP NC thin films, remains a great challenge. Herein, we report a resurfacing strategy via pseudohalogen ions to mitigate detrimental halide ion migration, aiming to stabilize PNC LEDs. We employ a thiocyanate solution processed post-treatment method to efficiently resurface CsPbBr3 NCs and demonstrate that the thiocyanate ions can effectively inhibit bromide ion migration in LHP NC thin films. Owing to thiocyanate resurfacing, we fabricated LEDs with a high EQE of 17.3%, a maximum brightness of 48000 cd m-2, and an excellent operation half-life time.

On the Use of Pseudo-Protic Ionic Liquids to Extract Gold(III) from HCl Solutions.

Solvent extraction of gold(III) from HCl media using pseudo-protic ionic liquids (PPILs) dissolved in toluene as the extractant phase is investigated. Three PPILs are generated from the reaction of commercially available amines and 1 M HCl solution and named as pri-NH2H+Cl- (derived from the primary amine Primene 81R), sec-NHH+Cl- (derived from the secondary amine Amberlite LA2) and ter-NH+Cl- (derived from the tertiary amine Hostarex A327). In the above structures, -NH2H+Cl-, -NHH+Cl- and -NH+Cl- represented the active groups (anion exchangers) of the respective PPIL. In the case of gold(III) extraction, the experimental variables investigated included the equilibration time (2.5-30 min), temperature (20-60 °C), HCl concentrations (1-10 M) in the aqueous phase, gold(III) concentration (0.005-0.05 g/L) in this same phase, and PPILs concentrations in the organic phase. From the experimental data, and using the Specific Interaction Theory, the interaction coefficients (ε) for the pair AuCl4-, H+ are estimated for the systems involving the three PPILs. Gold(III) is recovered from the metal-loaded organic phases using sodium thiocyanate solutions, and from these, gold is finally recovered by the precipitation of zero-valent gold (ZVG) nanoparticles.

TRANSFER OF A DROP MATERIAL DURING THE PRIMARY CAVERN FORMATION

The transfer of a freely falling drop matter in the bulk of a target fluid at rest at the stage of primary cavity formation was traced by high-speed video recording for the first time. In the experiments, drops of water, ink solution (diluted in a ratio of 1:100) or a saturated solution of baking soda with a diameter of D = 0.43 cm fell with velocity of U = 3.1 m/s into fresh water or a 20% ammonium thiocyanate solution in the splash formation mode. In all experiments, the wall of the growing cavity was penetrated by thin fibers containing drop matter, which form an intermediate fine-structured layer. After the end of the fiber growth stage with a duration of 7–8 ms, the diffusion smoothed the concentration gradients, a liquid shell of intermediate density with a thickness of 1.5 to 0.7 mm was formed around the growing cavity. The shell is separated by a sharp boundary from the target fluid. A new group of inclined fibrous loops was formed in the wake of the collapsing cavity.

Effect of Some Environmental and Stainless Steel Metallurgical Variables on the Values of Degree of Sensitization Measured by the Double Loop-Electrochemical Potentiokinetic Reactivation Test

The double loop-electrochemical potentiokinetic reactivation (DL-EPR) method estimates the degree of sensitization in stainless steels with ir/ia, the ratio of peak current densities during reactivation (reverse) and activation (forward) scans. Beyond sensitization level, other metallurgical variables, like inclusion content and cold work, or testing environment variables, like solution deaeration and solution aging, could potentially affect ir/ia. Austenitic stainless steel Types AISI 304 (UNS S30400) and AISI 303 (UNS S30300) were tested to assess the effect of those secondary variables on ir/ia. AISI 303 stainless steel had similar chromium and nickel contents to AISI 304, but higher contents of sulfur, resulting in a higher volume fraction of inclusions. ir/ia increased with inclusion content and decreased with cold work in AISI 304 stainless steel. In all cases, the DL-EPR test could discriminate solubilized from thermally aged specimens because confidence intervals of respective samples never overlapped. The testing solution was 0.5 mol/L H2SO4 + 0.01 mol/L KSCN, (sulfuric acid and potassium thiocyanate solution) and a common practice is to freshly prepare it just before the test. Measurements are usually performed under deaerated conditions. However, deployment of the technique, especially in the field, could be facilitated if naturally aerated solutions prepared in advance in a laboratory can be used. The oxygen content in solution and solution aging did not have a statistically significant effect on ir/ia.

Validation of a colorimetric method for measuring urinary thiocyanates applicable to cyanide poisoning

The aim of this work is the validation of a spectrophotometric method for the measurement of urinary thiocyanates. In acidic pH, thiocyanate ions give, with ferric nitrate, a red colour corresponding to 400 nm. The reagents required for the assay were: a stock solution of thiocyanate prepared by dissolving ammonium thiocyanate (NH4SCN) in distilled water, a solution of ferric nitrate [Fe(NO3)3, 9H2O] at 1% prepared by dissolving ferric nitrate in distilled water, a solution of hydrochloric acid (10%) prepared by diluting hydrochloric acid in distilled water. A standard calibration range and three validation ranges were prepared and analysed every day for three days. A total of 3 standard ranges and 9 validation ranges were performed. A blank is used to tare the absorbance to zero, treating a 1 mL blank urine, in the same way as the range points. The evaluation examined specificity, accuracy, fidelity and linearity. The Accuracy Profile was established with an acceptable deviation λ set at ± 15%. The probability β was set at 80%. The validated method is linear in the concentration range varying between 0.232 g/L and 0.5 g/L. The response function used is Y = a3X (The acceptable deviation λ has been set at ± 15%. The probability β has been set at 80%). The coefficient of variation (CV) of repeatability varies between 0.123 and 0.215%. The limit of quantification is 0.232 g/L. Cyanide is quickly converted by sulfurization to thiocyanate (SCN−). This is the main route of cyanide biotransformation. Thiocyanate is excreted primarily in the urine. This method, which is easy to implement in laboratories with limited resources and useful as a marker for acute cyanide poisoning, can be used as a diagnostic aid.

Epidemiological Studies of Children's Gut Microbiota: Validation of Sample Collection and Storage Methods and Microbiota Analysis of Toddlers' Feces Collected from Diapers.

The composition of human gut microbiota influences human health and disease over the long term. Since the flora in specimens can easily change at ambient temperature outside the body, epidemiological studies need feasible methods of stool specimen collection and storage to be established. We aimed to validate two methods: feces frozen-stored in tubes containing guanidine thiocyanate solution for two months after collection (Method B), and feces excreted in diapers and frozen-stored (Method C). Validation was by comparison with a gold standard Method A. Bacterial flora of five adults were sampled and stored by all three methods. Bacterial composition was examined by amplicon sequencing analysis. Bland–Altman analyses showed that Methods B and C might change relative abundances of certain bacterial flora. Thereafter, we analyzed the bacterial flora of 76 toddlers (two age groups) in stools sampled and processed by Method C. The diversity indices of toddlers’ flora were less than those of adults. The relative abundance of some bacteria differed significantly between children aged 1.5 and 3 years. The specimen collection and storage methods validated in this study are worth adopting in large-scale epidemiological studies, especially for small children, provided the limited accuracy for some specific bacteria is understood.

Gold Adsorption from Pregnant Thiocyanate Solution Using Activated Carbon

Gold adsorption from dissolution of gold ore concentrate at Tanggamus mine using activated carbon from thiocyanate leached solution was investigated in this research. As the pollution of the environment has been raising accordingly with the increasing of cyanidation, thiocyanate is becoming current alternative and less toxic reagent in terms of gold dissolution. However, the research was still limited. The development and implementation of thiocyanate in gold extraction is the core of this research. Solvent and oxidizer concentrations (KSCN and FeCl3) were used in this study with 0.3 M and 0.1 M of concentration, respectively. Adsorption process was carried out for 4 hours using 100 ml of filtrate with activated carbon addition. Adsorption of thiocyanate complex gold compounds was studied by varying the size of activated carbon, the concentration of activated carbon, and the rotary speed of bottle roll. At-10+20# of activated carbon size, the %-adsorption obtained was 93.65%. Meanwhile, 94.31% of gold adsorption was achieved with the concentration of activated carbon at 1500 mg/l and 93.37% of gold adsorption was reached with the rotary speed of 150 rpm. Pseudo second order was applied to calculate the kinetics of gold adsorption with the R2 value obtained was 0.9999.

Cyclic degradation of thiocyanate in cyanide barren solution by manganese oxides

This study describes the degradation of thiocyanate by manganese oxides. With NaOH and CaO as precipitating agents, two manganese oxides, i.e. MnOx/Na and MnOx/Ca, were prepared using MnSO4·H2O as Mn resource and air as oxidant. The influence of various reaction parameters like pH, manganese oxide dosage and initial thiocyanate concentration were systematically investigated with MnO2 as a reference. Based on an entire process dynamics model and the initial kinetics analysis, the rate-limiting step (formation of the precursor complexes) and reaction orders were derived independently. The reaction pathway was detected and the formation of the precursor complexes between surface positively charged manganese oxides and thiocyanate was the first step of thiocyanate degradation, after which electron transfer occurred within the precursor complexes and intermediate products were formed, which were rapidly released to produce SO42− and CN−, and some of the CN− would be further oxidized to HCO3− and NH4+. The formation of precursor complexes is related to the charge distribution on the surface of the manganese oxide and the protonation of thiocyanate in solution, which makes the lower pH favorable. The results of cyclic treatment of cyanide barren solution showed good regenerability of MnOx/Ca and gradual reduction of undesired secondary salts (especially SO42− ions) in the degraded liquid, which made it a potentially reliable technique to regulate the thiocyanate concentration during the cyanidation process of gold ores economically.

Synthesis, Biological Activity of Trimethoprim derivative and the Complexes

This study demonstrates the synthesis of new ligands derived from trimethoprim with their Iron(III) and copper (ll) complexes. At the beginning, preparing new ligands, the first namly N, N’-(5-(3,4,5-trimethoxybenzyl)pyrimidine-2,4-diyl)bis(3,4,5-trihydroxy benza mide)and the Ligand second namly N, N’-(((5-(3,4,5-trimethoxybenzyl)pyrimidine-2,4-diyl) bis(azanediyl))bis(carbonthioyl) bis(3,4,5-trihydroxybenzamide) which prepared by nucleophilic addition of trimethoprim to Gallic chloride and prepared also by nucleophilic addition of trimethoprim to the Solution of Ammonium thiocyanate and Gallic chloride. Ferric Ion (III) and Copperic Ion (ll) complexes have been prepared with molar ratio [1:2]. The synthesis ligands have been characterized by Uv-Visible, FT-IR, 1HNMR, 13CNMR and EI-mass, while the complexes have been characterized by elemental analysis, Uv-Visible, FT-IR, Conductivity measurements and thermogravemtric (TgA) analysis. The ligands acts as multiple sites coordinating with ferric ion and copper ion, Via lone pair of nitrogen atom of NHC= and phenolic oxygen. In Vitro, the ligands and complexes have been tested for their growth Inhibitory activity against Gram negative bacteria Salmonella Spp and Gram Positive Staphylococcus Spp. The results of the test indicate that the synthesized compounds possessed a high inhibition effectiveness comparative with trimethoprim.