Synthesis, Biological Activity of Trimethoprim derivative and the Complexes

Abstract

This study demonstrates the synthesis of new ligands derived from trimethoprim with their Iron(III) and copper (ll) complexes. At the beginning, preparing new ligands, the first namly N, N’-(5-(3,4,5-trimethoxybenzyl)pyrimidine-2,4-diyl)bis(3,4,5-trihydroxy benza mide)and the Ligand second namly N, N’-(((5-(3,4,5-trimethoxybenzyl)pyrimidine-2,4-diyl) bis(azanediyl))bis(carbonthioyl) bis(3,4,5-trihydroxybenzamide) which prepared by nucleophilic addition of trimethoprim to Gallic chloride and prepared also by nucleophilic addition of trimethoprim to the Solution of Ammonium thiocyanate and Gallic chloride. Ferric Ion (III) and Copperic Ion (ll) complexes have been prepared with molar ratio [1:2]. The synthesis ligands have been characterized by Uv-Visible, FT-IR, 1HNMR, 13CNMR and EI-mass, while the complexes have been characterized by elemental analysis, Uv-Visible, FT-IR, Conductivity measurements and thermogravemtric (TgA) analysis. The ligands acts as multiple sites coordinating with ferric ion and copper ion, Via lone pair of nitrogen atom of NHC= and phenolic oxygen. In Vitro, the ligands and complexes have been tested for their growth Inhibitory activity against Gram negative bacteria Salmonella Spp and Gram Positive Staphylococcus Spp. The results of the test indicate that the synthesized compounds possessed a high inhibition effectiveness comparative with trimethoprim.