Thiobarbituric Acid Reactive Substances Research Articles

Influence of edible flowers on the physicochemical and oxidative stability of raw ground beef patties

This study investigates the antioxidant and physicochemical characteristics of raw ground beef patties and raw ground beef patties treated with varying percentages of roselle (Hibiscus sabdariffa L.) and rose (Rosa canina L.) powders during 7 days of storage at 4°C. The analysis included key parameters such as antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, lipid oxidation through 2-thiobarbituric acid reactive substances (TBARS), protein oxidation markers (carbonyls, Schiff bases, and free thiols), water-holding capacity, pH, color, and texture. Both roselle and rose powders enhanced the antioxidant capacity of the patties, reducing oxidative markers (TBARS, carbonyls, Schiff bases, and free thiols) during storage. Additionally, improved water-holding capacity and reduced pH were observed across all treated patties, with minimal impact on texture. However, while roselle powder showed beneficial effects, patties treated with rose powder exhibited superior overall results. The more favorable outcomes in rose-treated patties, particularly in oxidative stability and physicochemical properties, can be attributed to the higher concentrations of bioactive compounds, such as phenolic acids and flavonoids, present in rose powder. These compounds likely contributed to enhanced free radical scavenging activity, providing stronger protection against lipid and protein oxidation. Furthermore, rose powder maintained more stable color and physicochemical properties, with patties showing acceptable color and minimal texture degradation by the 7th day of storage. These findings highlight the potential of rose powder as a highly effective natural additive for extending the shelf life and preserving the quality of ground beef patties, positioning it as a promising ingredient for future applications in the food industry.

The Effects of Cooking Methods on Gel Properties, Lipid Quality, and Flavor of Surimi Gels Fortified with Antarctic Krill (Euphausia superba) Oil as High Internal Phase Emulsions

In this study, silver carp surimi products enriched with Antarctic krill oil high internal phase emulsions (AKO-HIPEs) were cooked using steaming (STE), microwave heating (MIC), and air-frying (AIR), respectively. The gel and flavor properties, lipid quality and stability were investigated. Compared to the MIC and AIR groups, the STE surimi gel added with HIPEs had better texture properties, exhibiting higher water-holding capacity and a more homogeneous structure, while the air-frying treatment resulted in visually brighter surimi products. The degree of lipid oxidation during cooking was in an order of STE < MIC < AIR as determined by electron paramagnetic resonance spectrometer and thiobarbituric acid reactive substances. HIPE-added surimi gels retained more nutrients and flavor when cooked by AIR compared to STE and MIC. Results imply that the texture properties and lipid stability of surimi products fortified with AKO-HIPEs were better than those of the oil group under any cooking method. In conclusion, surimi products added with AKO-HIPEs had better gel properties and retained more fatty acids and flavor than AKO-SO.

Antioxidant and anti-inflammatory potential of vanillic acid improves nephrotoxicity induced by sodium arsenite in mice

ABSTRACT This study examined the potential of vanillic acid (VA) to protect against renal oxidative stress and inflammation caused by sodium arsenite (SA) in mice. Mice were assigned to five groups: control, VA (100 mg/kg), SA (50 ppm in drinking water for 8 weeks), and SA + VA (50 and 100 mg/kg orally in the 7th and 8th weeks). After the experiment was ended, the Mice were sacrificed and serum and renal tissue samples were collected for additional assessments. Treatment with VA suppressed SA-induced increase in blood urea nitrogen and creatinine in the serum. Furthermore, renal histological damage induced by SA administration was ameliorated with VA treatment. Also, the increase in the level of lipid peroxidation marker (thiobarbituric acid reactive substances) along with the reduction in total thiol levels and the diminished activities of antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase) in the renal tissue of SA-treated mice were effectively reversed following treatment with VA. The results exhibited that the VA-treated groups (50 and 100 mg/kg) mitigated the elevation of inflammatory markers in kidney tissue (tumor necrosis factor-α and nitric oxide) in SA-exposed mice. Our research findings indicate that VA could be a potential therapeutic agent for the management of SA-associated nephrotoxicity.

Phosphate and two-stage cooking: Impact on lipid oxidation and tenderness in sous-vide beef semitendinosus.

The study investigated phosphate's effects on tenderness and lipid oxidation in beef cooked sous-vide. Semitendinosus beef cuts were treated with 0.15% and 0.3% sodium tripolyphosphate (STPP), and plain water (0% STPP). These cuts were then sous-vide cooked at two different temperatures, 60 °C and 70 °C, for two different durations, 3 h and 6 h. Analytical techniques including Warner-Bratzler shear force (WBSF), pH, thiobarbituric acid reactive substances (TBARS), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FTIR) were employed. STPP concentration did not yield a statistically significant impact on the pH and FTIR spectrum of sous-vide beef (p > 0.05). However, STPP markedly enhanced tenderness by loosening the myofibril structure (observed in SEM) and weakening myosin and actin bands (observed in SDS-PAGE). This improvement led to lower WBSF values with 0.3% STPP, particularly noticeable at 60 °C for 6 h. Additionally, 0.3% STPP delayed lipid oxidation, evidenced by lower oxidation values after 4 weeks of refrigerated storage at 4 °C. These findings highlight STPP's potential to improve tenderness and lipid oxidation stability in sous-vide beef.

The impact of N-acetylcysteine on lactate, biomarkers of oxidative stress, immune response, and muscle damage: A systematic review and meta-analysis.

N-acetylcysteine (NAC) is a compound whose mechanism of action is intricately linked to the provision of cysteine for glutathione synthesis. It has been used in medicine and has also made significant inroads into sports, as it can modify the levels of several biomarkers, including those of oxidative processes, inflammation and muscle damage after exercise. Because the effectiveness of NAC supplementation is unclear, the primary objective of the present study was to perform a meta-analysis elucidating how NAC supplementation alters the concentrations of GSH (glutathione), GSSG (glutathione disulfide), TBARS (thiobarbituric acid reactive substances), IL-6 (interleukin 6), TNF-α (tumour necrosis factor alpha), CK (creatine kinase), lactate, and muscle soreness after physical exertion. Suitable studies were searched for from February to September 2023, and the results of those included (n = 20) indicate that NAC supplementation significantly diminishes both muscle soreness (p = 0.03; the mean difference (MD) of NAC's effect was -0.43 with a 95% confidence interval (CI), -0.81, -0.04) and lactate concentrations after exercise (p = 0.03; the MD -0.56 mmol/L; 95% CI, -1.07, -0.06). A substantial decrease was observed in concentrations of IL-6 (p = 0.03; the standardized MD (SMD) was -1.71; 95% CI, -3.26, -0.16) and TBARS (p = 0.02; SMD was -1.03, 95% CI, -1.90, -0.15). Furthermore, an elevation in GSH concentration was observed following supplementation. However, we saw no significant effect of NAC on TNF-α, CK or GSSG concentrations. NAC supplementation holds promise for attenuating muscle soreness, lactate, TBARS and IL-6 concentrations and increasing GSH level following physical exertion.

Genotoxicity and oxidative stress assessment among farmers occupationally exposed to pesticides in El-Behira Governorate, Egypt

ABSTRACT Background Recently, pesticide usage has rapidly increased worldwide and consequently, there is a great worry about their adverse health effects. In literature, there is paucity of research regarding the relationship between occupational pesticide exposure and genotoxicity. The current study aimed to detect sociodemographic and occupational characteristics of the participants and to assess biomarkers of genotoxicity and oxidative stress and reveal their association with exposure to pesticides. Methods A comparative cross-sectional approach was carried out at a randomly selected village where 58 farmers exposed to pesticides were compared with another 58 individuals not exposed to pesticides. Participants were interviewed; blood samples were withdrawn for measuring the following: deoxyribonucleic acid (DNA) fragmentation percentage, serum thiobarbituric acid reactive substances (TBARS) level, serum activity levels of glutathione reductase (GR), catalase (CAT), superoxide dismutase (SOD), and butyrylcholinesterase (BChE) enzymes. Results Farmers had significantly higher levels of DNA fragmentation, TBARS, and SOD [8.07 ± 2.38, 1.42 (0.79), and 350.09 (30.03), respectively] than the comparison group [4.41 ± 1.35, 0.37 (0.72), and 281.25 (130.37), respectively]; While they had a lower GR, CAT, and BChE levels [14.53 ± 8.53, 21.7 (41.04), and 2707.77 ± 651.24, respectively] than the comparison group [24.92 ± 9.44, 37.8 (41.72), and 5694.3 ± 1175.76, respectively]. Exposure to pesticides was found to be independently associated with an increase in the percentage of DNA fragmentation by 3.146%; an increase in TBARS level by 0.717 nmol/dL; an increase in the SOD enzyme activity level by 72.614 IU/mL and a decrease in the GR enzyme activity level by 10.094 IU/mL. Conclusion Pesticide exposure is associated with genotoxicity and oxidative stress.

Preparation of Physalis alkekengi L. calyx total flavonoids-chitosan composite film and its effect on preservation of chilled beef.

In the present study, Physalis alkekengi L. calyx total flavonoids (PCTF) were extracted using the ultrasound-assisted ethanol method and separated and purified using macroporous resin AB-8. Physalis alkekengi L. calyx total flavonoid-chitosan (PCTF-CS) composite films containing 0.05 %, 0.10 % and 0.15 % PCTF were prepared using the purified PCTF laminated with chitosan (CS) and compared with single CS films, respectively, to investigate their mechanical properties, barrier properties, optical properties, microstructure, crystallography, thermal stability, water contact angle, particle size and zeta potential, antioxidant property, antimicrobial property, and preservation effect on chilled beef. The PCTF-CS films with PCTF additions had darker colors and higher mechanical and barrier properties than the CS films. In addition, the addition of PCTF improved the antioxidant and antimicrobial properties of the CS films. It enhances the freshness retention of fresh beef, and effectively inhibits the rise of weight loss, pH, total bacteria, total volatile basic nitrogen, and thiobarbituric acid reactive substances in beef, prolonging the shelf life of beef. These results indicate that the addition of PCTF can provide CS films with superior functional properties and bioactivities and that PCTF-CS composite films are a potential and promising packaging material for food preservation.

Bariatric surgery blunts nitrate-mediated improvements in cardiovascular function of overweight women by interfering with gastric S-nitrosothiol formation.

Inorganic nitrate (NO3-) and nitrate-rich foods have been shown to exert antioxidative effects and lower blood pressure in experimental animal models and human clinical studies. The specific handling of nitrate, including its enterosalivary recirculation, secretion into saliva, oral microbial reduction to nitrite (NO2-), and the pH-dependent nitrosative capacity in the stomach have all been recognized as being important for nitrate's beneficial effects. Obesity is of major health concern worldwide and associated with increased cardiovascular risk; whether nitrate lowers blood pressure and improves endothelial function in this setting has not been investigated. We here tested the hypotheses that i) nitrate elicits cardiovascular benefits in overweight women; and ii) these beneficial effects would be diminished in women who underwent bariatric Roux-en-Y gastric bypass (RYGB) surgery. Our controlled clinical trial included 15 women with prior RYGB surgery and 15 overweight female controls. All participants received a single dose of 0.1mmol/kg/day nitrate in the form of a beetroot extract for 14 days. Blood collection, 24-h ambulatory blood pressure measurements and endothelial function tests were performed before and after nitrate treatment. Plasma nitrite, nitrate, and S-nitrosothiol (RSNO) concentrations were determined by ozone-based reductive chemiluminescence while thiobarbituric acid reactive substances (TBARS) and total antioxidant capacity (TAC) were measured using plate-reader based assays. Nitrate reduced blood pressure and improved endothelial function in controls, but not in women with prior bariatric surgery. Nitrate also increased circulating nitrate/nitrite and RSNO levels in controls, but the latter was blunted following RYGB surgery despite even larger increases in nitrite concentrations. Similarly, nitrate increased antioxidant responses in controls but not in women with prior bariatric surgery. This is the first study to show that nitrate exerts beneficial cardiovascular effects in obesity and that the morphological/functional modifications elicited by RYGB surgery abrogates nitrate's effectiveness by preventing gastric RSNO formation.

Hydrogen gas enhances Arabidopsis salt tolerance by modulating hydrogen peroxide-mediated redox and ion homeostasis.

Hydrogen gas (H2) plays a crucial role in mitigating salt stress in plants, but the underlying mechanisms is largely unknown. Herein, we employed the pharmacological, molecular, and genetic approaches to investigate the positive roles of hydrogen peroxide (H2O2) in endogenous H2-induced salt tolerance of Arabidopsis thaliana. H2-induecd salt tolerance of CrHYD1 (hydrogenase 1 gene from Chlamydomonas reinhardtii) transgenic Arabidopsis was blocked by H2O2 scavenger or NADPH oxidase inhibitor. When RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) genes (AtrbohD or AtrbohF) were mutated, salt sensitivity of CrHYD1/atrboh (especially CrHYD1/atrbohD) hybrids was increased, but diminished by exogenous H2O2 administration. Salt-stimulated endogenous H2 enrichment consequently resulted in the rapid reactive oxygen species (ROS) accumulation under early salt stress, and the expression of AtrbohD (especially) and AtrbohF in CrHYD1 plants was higher than those in the wild-type (WT), suggesting that endogenous H2 could induce Atrboh-dependent ROS burst to respond salt stress. Further, H2-induced less 3,3'-diaminobenzidine (DAB) and nitro blue tetrazolium (NBT) stain in CrHYD1 plants was reversed under salt stress when either H2O2 was removed or Atrbohs were mutated, which could be explained by higher H2O2 and thiobarbituric acid reactive substances (TBARS) levels, as well as lower antioxidant enzyme activity. Additionally, H2-induced Na+ discharge and K+ accumulation in CrHYD1 plants under salt stress were blocked by either H2O2 removal or Atrboh knockout, which was validated by higher Na+/K+ ratios and lower ion transport-related gene expression. Our findings not only elucidate that endogenous H2 enhanced Arabidopsis salt tolerance by reestablishing H2O2-dependent ion and redox homeostasis, but provide new insights into the mechanisms of plant salinity responses.

Active and Intelligent Packaging Technology for Hilsa (Tenualosa ilisha)

During long marketing chain, Hilsa rapidly lose quality even in chilled condition. When purchase, consumers often get confused to differentiate between stale and fresh Hilsa. Four different active packages; namely, G1 (commercial oxygen absorber + nisin), G2 (commercial oxygen absorber + chitosan), G3 (glucose oxidase + nisin), and G4 (glucose oxidase + chitosan) were manufactured and tested to overcome these troubles. In case of thiobarbituric acid reactive substances (TBARS) content, G3 had lowest TBARS value followed by G4, G1 and G2, while control samples had the highest TBARS value. In regard to protein retention, all active packagings showed higher protein retention than control. Bacterial load was highest (4.87 log CFU/g) in control samples while active packagings had 2.29-3.96 log CFU/g. In case of total volatile basic nitrogen (TVB-N) content, G3 had minimum TVB-N content followed by G4, G1 and G2, while control samples had the highest TVB-N content. Phenol red dyed paper strips, which were used as intelligent indicator, did not change bright orange colour in active packages while turned into yellow and pale yellow in control and unacceptable samples, respectively. Active packagings were able to keep quality of Hilsa good for at least 28 days. Described active packagings technologies should be applied to enhance shelf-life of fishery products.

Physiological and biochemical responses of soybean seedlings (Glycine max L.) to α-tocopherol treatment under salt stress

Background. Salinity is one of the most important factors affecting the growth and productivity of plants. It creates stressful conditions for legumes at the initial phases of ontogenesis. The prospects of exogenous α-tocopherol usage to increase the salt resistance of Glycine max L. were studied. The aim of the work was to study the influence of salt stress and α-tocopherol on the growth of soybean and indicators characteri­zing the antioxidant system functioning. Materials and methods. Soybean seedlings of the Oksana variety were the objects of our study. Seeds of the control group were soaked in distilled water, seeds of the experimental groups – in α-Toc acetic solutions in concentrations (0.01; 0.1; 0.5; 1.0 g/L). A 100 mM sodium chloride solution was used to create salinity. The samples of cotyledons, hypocotyls and primary roots of 10-day-old soybean were used for determination of biochemical parameters. Germination of seeds, raw weight, length of soybean hypocotyls and roots, content of TBA-reactive substances, carbonyl groups of oxidatively modified proteins, proline, catalase and ascorbate peroxidase activity were determined. Results. It was established that salinity caused inhibition of soybean seed germination and growth. Pretreatment of soybean seeds with α-Toc (0.1 and 0.5 g/L) effectively increased germination and improved growth indicators of soybean. An increase in TBA-reactive substances and oxidatively modified proteins in hypocotyls and roots of salt-stressed plants was recorded. α-Toc reduced the level of peroxidation and oxidatively modified proteins of soybean seedlings under salinity. Proline synthesis increased during the development of the salt stress reaction, and its accumulation is an adaptive response of soybean plants. The pretreatment of α-Toc caused a significant increase of proline and stimulated catalase and ascorbate peroxidase activity in soybean seedling tissues under salt stress. However, a high concentration of α-Toc (1.0 g/L) slowed down the activity of antioxidant enzymes. Conclusions. Our study suggests the participation of α-Toc in the formation of legumes salt resistance. The α-Toc pretreatment of soybean seeds improved germination and enhanced growth processes, normalized the oxidative state of the salt-stressed soybean seedlings by inhibiting peroxidation and reducing the degree of oxidatively modified proteins, stimulating the activity of antioxidant enzymes, and increasing the content of proline.

Combination of Essential Oils and Extracts Enriched with Tocopherols in Diet of Holstein Steers and Its Effect on Ruminal Fermentation, Antioxidant and Anti-Inflammatory Response, Feed Efficiency and Fatty Acid Profile in Meat

Background: This experiment evaluated whether the combination of essential oils (thyme, rosemary, orange) with extracts of Cinnamomum and Quillaja saponaria (enriched with tocopherols) in steers’ diet has positive effects on the health, digestibility, growth, oxidative stress, and fatty acid profile of meat. Methods: The experiment included 24 Holstein steers, divided into two groups, randomly divided (treated = phytogenic mixture (n = 12); control = monensin (n = 12)), undergoing a growth phase of 120 days and 90 days of finishing in individual pens, with a monthly collection of blood, rumen fluid, feces, and feed. Results: In the finishing phase (days 150 to 210), there was a tendency (p = 0.07) toward more significant average daily weight gain when cattle consumed a phytogenic mixture than monensin. The use of the phytogenic product showed greater feed efficiency in the period from 150 to 240 days (p < 0.05), with higher levels of short-chain fatty acids (SCFAs), without differences in the composition of these SCFAs between the control and treated groups, with a reduction in apparent digestibility in the treated group. In blood parameters, the treated group showed higher erythrocyte counts (p < 0.05) without changes in leukocytes, lymphocytes, granulocytes, monocytes, and platelets. The phytogenic treatment demonstrated higher total protein and globulin levels, with increased glucose levels on days 30 and 210 (p < 0.05). Ceruloplasmin and haptoglobin levels increased in the treated group without changes in ferritin, transferrin, C-reactive protein, IgA, and heavy-chain immunoglobulins (p < 0.05). A reduction in oxidative stress was observed using the phytogenic agent, with a marked decrease in the levels of reactive oxygen species (ROS) and carbonyl proteins, with a tendency to increase glutathione S-transferase (p < 0.05). In meat, the phytogenic reduced saturated fatty acids with increased polyunsaturated fatty acids and increased the omega 6/omega 3 ratio. Lower levels of thiobarbituric acid reactive substances and ROS were detected in the meat combined with a higher concentration of total thiols in the treated group than in the control group. Conclusions: The use of the phytogenic in steers’ diets has shown to be a viable candidate for replacing monensin, presenting equal or superior performance results with systemic modulating effects on oxidative stress, meat quality, and serum and biochemical parameters that contribute to more substantial health and efficiency.

The Impact of an Edible Composite Coating Formulated with Farsi Gum and Iranian Black Cumin Essential Oil Emulsion or Nanoemulsion, Alongside Low-Dose Gamma Irradiation, on the Quality of Chilled Turkey Breast

Background: Hurdle technology is an effective method for preserving muscle food products and extending their shelf life. Objectives: This study evaluated the combined effect of Farsi gum (FG) coating impregnated with black cumin essential oil (BCEO) emulsion or black cumin nanoemulsion (BCNE) and low-dose gamma irradiation (GIR) at 2 kGy on the quality of turkey breast over 21 days of chilled storage. Methods: Turkey breast samples were divided into eight groups: Control, FG, GIR, FG + GIR, FG + BCEO, FG + BCNE, FG + BCEO + GIR, and FG + BCNE + GIR. The samples were assessed for microbial, physicochemical, and sensory attributes throughout 21 days of chilled storage. Results: The growth of mesophilic and psychrotrophic bacteria, Enterobacteriaceae, and lactic acid bacteria (LAB) was inhibited in all treated groups compared to the control and FG groups during the storage period. The treatments also curbed the increase in pH, total volatile basic nitrogen (TVB-N), thiobarbituric acid reactive substances (TBARS), and protein carbonyls in the turkey breast. The most effective treatment for controlling microbial growth, minimizing physicochemical changes, and extending the storage life of turkey breast was the combination of FG + BCNE + GIR. Conclusions: The combination of active FG coating containing BCNE and low-dose GIR offers effective long-term preservation of turkey breast during chilled storage.

Development of anti-bacterial bio-transfer double sheet layer of polyvinyl alcohol/carboxymethyl cellulose films infused with Astragalus tribuloides leaf extract for beef burgers preservation

This study was conducted to develop biodegradable films using a combination of carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA) and purified leaves extract of Astragalus tribuloides (ATE). Various traits of the films, including their morphology description, thermal behavior, tensile/elongation properties and physical characteristics were examined. The scanning electron microscope (SEM) photographs showed smooth surface with small amounts of ATE, but rougher with higher concentrations of 1.4 %. The Fourier-transform infrared spectroscopy (FTIR) showed a direct relationship between the ATE extract and the PVA/CMC matrix. The films also showed thermal stability behaviors. The study found that the addition of ATE up to 0.8 % caused the films to become opaquer in color and raised their opacity up to 3.909. As a result, the films exhibited reduced moisture absorption (8.21 %) and solubility (27.11 %), making them retard penetrating water vapor up to (1.785 × 10−10 g.m−1 s-1 Pa−1) and could preserve the thiobarbituric acid reactive substances (TBARS) and overall color discrepancies of burger in refrigerated storage.

Levocabastine ameliorates cyclophosphamide-induced hepatotoxicity in Swiss albino mice: modulation of Nrf2, NF-κB p65, cleaved caspase-3 and TGF-β signaling molecules.

Cyclophosphamide (CP)-induced hepatotoxicity is a significant problem in clinical settings. This study aimed to evaluate the protective effect of levocabastine (LEV) on CP-induced hepatotoxicity in Swiss albino mice. Mice were given CP (toxic drug) 200mg/kg, i.p., once on the 7th day, and LEV 50 and 100µg/kg, i.p., and fenofibrate (FF) 80mg/kg, p.o., daily for 14 days. On the 15th day, blood and liver samples were collected to assess biological parameters. CP 200mg/kg caused hepatotoxicity due to oxidative stress, inflammation, apoptosis, and fibrosis as manifested by a reduction in catalase, reduced glutathione (GSH), superoxide dismutase (SOD), and an increase in thiobarbituric acid reactive substance (TBARS), nitrite, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), transforming growth factor-beta 1 (TGF-β1), interleukin-1β (IL-1β), alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and gamma-glutamyl transferase (GGT) levels. Cleaved caspase-3 and nuclear factor kappa-B (NF-κB) expression was also increased and nuclear factor erythroid 2-related factor (Nrf2) expression was decreased as confirmed by Immunohistochemical analysis. It also caused histopathological abnormalities and fibrosis as manifested by Hematoxylin-Eosin (H&E) and Masson's trichrome (MT) staining. These alterations were returned to almost normal when treated with LEV 100µg/kg and FF 80mg/kg. Thus, LEV protected CP-induced hepatotoxicity by reversing inflammation, apoptosis, fibrosis, oxidative stress, hepatic injury, and histopathological damages. LEV can be helpful as an adjuvant in cancer patients who are on CP treatment, to minimize toxicity. However, its role in in-vivo cancer model is further needed to be confirmed.

Influence of processing of seitan, tempeh, and firm regular tofu on protein and lipid oxidation and Maillard reaction products formation

The impact of production and cooking on protein oxidation, lipid oxidation and Maillard reaction products (MRPs) generation was evaluated for firm regular tofu, tempeh, and seitan, three plant-based alternatives to meat products. Tofu showed higher content of protein-bound carbonyls (25.5 ± 5.4 nmol/mg protein) indicating higher degree of protein oxidation, while seitan exhibited the lowest content of MRPs like N(6)-carboxymethyllysine, as revealed by LC-MS/MS. Through determination of the peroxide value and thiobarbituric acid reactive substances as well as analysis of volatile oxidation compounds by GC–MS, an overall higher lipid oxidation level was found in tofu. Likewise, a mean 92.2 % depletion of naturally occurring antioxidant tocopherol, assessed by HPLC-DAD, was observed in tofu. Furthermore, acrylamide was detected in tofu at 196.0 ± 15.4 μg/kg after frying at 190 °C. This study, therefore, concluded that practices used in tempeh and seitan processing are promising strategies to limit oxidation and Maillard reactions in food.

Effect of ultrasound-assisted water-holding agent on the quality of frozen stored beef

Beef was soaked in an ultrasonic-assisted water-holding agent to increase its water-holding capacity without sacrificing its quality. Storage at -18 °C for 0, 7, 14, 21 and 28 days, the effects of the water-holding agent and ultrasound on the quality of the beef were discussed, along with the centrifugal loss rate, pH, texture, water distribution, and thiobarbituric acid reactive substances (TBARS) and other indexes. The centrifugal loss of the control group was found to be 14.33 %, while the chitosan treatment had the lowest centrifugal loss. The centrifugal losses of compound phosphate, soybean dietary fiber, and chitosan were found to be 10.77 %, 11.86 %, and 12.10 %, respectively, over the 28-days freezing time. Furthermore, there was less water migration and a lower free water P22 value in the chitosan and compound phosphate treatment groups, indicating improved water maintenance capabilities. The highest TBARS value in the control group was 1.17 mg/kg, and the lowest value of compound phosphate was 0.91 mg/kg, indicating that the compound phosphate had a good effect on lipid peroxidation. The findings of this study offer valuable insights into the water-holding properties of water-holding agents in frozen beef and serve as a guide for using these agents in the food industry.

Exploring the association of dietary inflammatory index, disease progression and cellular integrity in chronic kidney disease: A cross-sectional study.

While the anti-inflammatory effects of specific nutrients are well established, there is limited research on the relationship between overall dietary patterns and chronic kidney disease (CKD). Therefore, we aimed to assess the association between dietary inflammatory index (DII) scores and biochemical and body composition parameters in pre-dialysis CKD patients. The DII scores of 143 pre-dialysis CKD subjects were calculated using 24-h recalls. Body composition was measured using bioelectric impedance-based InBody 770. Oxidative stress and total antioxidant capacity were measured using thiobarbituric acid reactive substances (TBARS), ferric-reducing antioxidant power (FRAP), and uric acid-independent FRAP (FRAP_UA) assays respectively. C-reactive protein (CRP) was selected as an inflammatory marker while other biochemical parameters were analysed using diagnostic kits. Subjects were categorized into early-stage CKD (ES; eGFR: 30 to 59 ml/min/1.73 m2) and late-stage CKD (LS; eGFR: < 30 ml/min/1.73 m2). The ES group had a lower extracellular to total body water ratio (p < 0.01) and higher levels of whole-body phase angle (p < 0.01) compared to the LS group. However, FRAP_UA was higher in the LS group (p < 0.05). For each increase in DII score by 1, eGFR decreased by 2.31 ml/min/1.73 m2 (p < 0.01) and phase angle by 0.11 degrees (p < 0.05). Consuming cereals and millets, other vegetables, roots and tubers, spices, poultry, oils and fats significantly contributed to the anti-inflammatory potential of the diet (p < 0.01). A pro-inflammatory diet was associated with disease progression and decreased cell integrity, underscoring the importance of its consideration in CKD management.

Early detection of beef-quality indicators using hyperspectral imaging combined with pixel-based segmentation method corresponding to fat and protein region

This study investigated the utilization of hyperspectral imaging (HSI) in conjunction with pixel-based segmentation to predict the thiobarbituric acid-reactive substances (TBARS) and volatile basic nitrogen (VBN) content in beef. Hyperspectral images were acquired in the visible near-infrared (VIS-NIR) and shortwave infrared (SWIR) ranges to examine temporal alterations in the fat and protein regions. A partial least squares discriminant analysis (PLS-DA) model was employed to segment fat and protein pixels, followed by a partial least squares regression (PLSR) model to predict the TBARS and VBN content from the segmented spectra. The SWIR range yielded the most accurate predictions, with an Rp2 of 0.899 for the early freshness indicators. Utilizing hyperspectral information from individual fat and protein pixels, rather than modeling the entire beef image, resulted in enhanced prediction accuracy for Rp2 of TBARS (0.814–0.899) and VBN (0.394–0.532) in the early stages of storage. These findings elucidate the potential of HSI with pixel-based segmentation as a nondestructive and real-time methodology for precise monitoring of beef freshness.

Effects of Chitosan-based Coating Enriched with Peanut (Arachis hypogaea) Skin Extract on Physicochemical, Microbiological and Sensory Characteristics of Beef Burger During Cold Storage

The current study aimed to create a chitosan-based coating enriched with peanut skin extract (PSE) at a concentration of 0.5, 1 and 1.5%, and evaluate its impact on the physicochemical, microbiological and sensory characteristics of fresh beef burger stored at 4±1°C for 15 days. All coated burgers had significantly higher L*, a* and b* values than the uncoated control during storage. Also, the results showed that application of chitosan coating enriched with 0.5, 1 and 1.5% PSE significantly increased total phenolic content (TPC) and antioxidant activity (AA), inhibited lipid oxidation as evaluated by thiobarbituric acid reactive substances (TBARS) and peroxide value (PV), retarded microbial growth and enhanced sensory characteristics of burger samples. Moreover, the positive effects of PSE on all investigated quality characteristics were proportional with the extract concentration. Therefore, using chitosan-based coating enriched with PSE enhances the stability of beef burger during cold storage and can be utilized in the meat industry.