Acidithiobacillus Thiooxidans Research Articles

Geochip 5.0 insights into the association between bioleaching of heavy metals from contaminated sediment and functional genes expressed in consortiums.

The heavy metal contamination in river and lake sediments endangers aquatic ecosystems. Herein, the feasibility of applying different exogenous mesophile consortiums in bioleaching multiple heavy metal-contaminated sediments from Xiangjiang River was investigated, and a comprehensive functional gene array (GeoChip 5.0) was used to analyze the functional gene expression to reveal the intrinsic association between metal solubilization efficiency and consortium structure. Among four consortiums, the Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans consortium had the highest solubilization efficiencies of Cu, Pb, Zn, and Cd after 15days, reaching 50.33, 29.93, 47.49, and 79.65%, while Cu, Pb, and Hg had the highest solubilization efficiencies after 30days, reaching 63.67, 45.33, and 52.07%. Geochip analysis revealed that 31,346 genes involved in different biogeochemical processes had been detected, and the systems of 15days had lower proportions of unique genes than those of 30days. Samples from the same stage had more genes overlapping with each other than those from different stages. Plentiful metal-resistant and organic remediation genes were also detected, which means the metal detoxification and organic pollutant degradation had happened with the bioleaching process. The Mantel test revealed that Pb, Zn, As, Cd, and Hg solubilized from sediment influenced the structure of expressed microbial functional genes during bioleaching. This work employed GeoChip to demonstrate the intrinsic association between functional gene expression of mesophile consortiums and the bioleaching efficiency of heavy metal-contaminated sediment, and it provides a good reference for future microbial consortium design and remediation of river and lake sediments.

A novel closed-loop biotechnology for recovery of cobalt from a lithium-ion battery active cathode material.

In recent years, the demand for lithium-ion batteries (LIBs) has been increasing rapidly. Conventional recycling strategies (based on pyro- and hydrometallurgy) are damaging for the environment and more sustainable methods need to be developed. Bioleaching is a promising environmentally friendly approach that uses microorganisms to solubilize metals. However, a bioleaching-based technology has not yet been applied to recover valuable metals from waste LIBs on an industrial scale. A series of experiments was performed to improve metal recovery rates from an active cathode material (LiCoO2; LCO). (i) Direct bioleaching of ≤0.5 % LCO with two prokaryotic acidophilic consortia achieved >80 % Co and 90 % Li extraction. Significantly lower metal recovery rates were obtained at 30 °C than at 45 °C. (ii) In contrast, during direct bioleaching of 3 % LCO with consortia adapted to elevated LCO levels, the 30 °C consortium performed significantly better than the 45 °C consortium, solubilizing 73 and 93 % of the Co and Li, respectively, during one-step bioleaching, and 83 and 99 % of the Co and Li, respectively, during a two-step process. (iii) The adapted 30°C consortium was used for indirect leaching in a low-waste closed-loop system (with 10 % LCO). The process involved generation of sulfuric acid in an acid-generating bioreactor (AGB), 2-3 week leaching of LCO with the biogenic acid (pH 0.9), selective precipitation of Co as hydroxide, and recirculation of the metal-free liquor back into the AGB. In total, 58.2 % Co and 100 % Li were solubilized in seven phases, and >99.9 % of the dissolved Co was recovered after each phase as a high-purity Co hydroxide. Additionally, Co nanoparticles were generated from the obtained Co-rich leachates, using Desulfovibrio alaskensis, and Co electrowinning was optimized as an alternative recovery technique, yielding high recovery rates (91.1 and 73.6% on carbon felt and roughened steel, respectively) from bioleachates that contained significantly lower Co concentrations than industrial hydrometallurgical liquors. The closed-loop system was highly dominated by the mixotrophic archaeon Ferroplasma and sulfur-oxidizing bacteria Acidithiobacillus caldus and Acidithiobacillus thiooxidans. The developed system achieved high metal recovery rates and provided high-purity solid products suitable for a battery supply chain, while minimizing waste production and the inhibitory effects of elevated concentrations of dissolved metals on the leaching prokaryotes. The system is suitable for scale-up applications and has the potential to be adapted to different battery chemistries.

Bio-solubilization of Rock Phosphate Using Acidophilic Bacteria and Effect of Bio-Organic-Phosphate Composite on the Growth of Wheat

Bio-solubilization of Rock Phosphate Using Acidophilic Bacteria and Effect of Bio-Organic-Phosphate Composite on the Growth of Wheat

Geopolymers functionalised by antibacterial zeolite against biocorrosion

Aluminosilicate geopolymer with remarkable chemical and biological resistance is a promising material solution to the persistent challenge of microbial induced concrete corrosion (MICC) in sewers. In this work, novel Sor-zeolite, assembled by using microporous 13X zeolite as a host of antimicrobial ion sorbate, is incorporated into fly ash-slag geopolymer to form bio-resistant materials with long-lasting efficacy and densified microstructure. The inclusion of pure zeolite and Sor-zeolite increase the strength and refine the pore structure of geopolymer pastes owing to the seeding nuclei effects during the geopolymerisation process. The enhanced dissolution of FA and slag by the seeding effects of pure zeolite and Sor-zeolite, accelerates and enriches the precipitation of geopolymeric gels, mainly a mixture of K-A-S-H and C-A-S-H (abbreviated as K-(C)-A-S-H). The Sor-zeolite-functionalised geopolymer possesses outstanding antibacterial properties against sulphur oxidising bacteria Acidithiobacillus thiooxidans, inhibiting biofilm formation on the surface and reducing corrosion depth. The main biodegradation products of geopolymers are gypsum and silica-rich gels, resulted from decalcification and dealumination of Si–O–Al and Ca–O bonds in K-(C)-A-S-H.

Urban biomining: lithium recovery from spent batteries through multi-step bioprocesses

Multi-step design to evaluate the bio-recovery of lithium from spent batteries was studied. The first step consisted of lithium extraction from spent batteries, using bacterial and fungal acid extrolites. The second step explored lithium recovery in the form of carbonate salts by using MICP (Microbial Induced Carbonate Precipitation) bacteria from Sporosarcina species. For lithium extraction (Step 1) sulfuric acid produced by sulphur oxidizing bacteria Acidithiobacillus thiooxidans was evaluated for its capacity to leach lithium. Extraction with biogenic sulfuric acid and with fungal bio- produ-cts (from Aspergillus sp. and Simplicillum sp. isolated at our facilities) were compared with commercial sulfuric acid. For biorecovery processes (Step 2), two type strains of Sporosarcina sp. were tested due to their capacity to precipitate lithium carbonate. Results showed fungal bioextracts gave a lithium leaching yield close to 60% and a global recovery yield of 27%. These observations are reported for the first time and lay the foundations for continuing the study and scaling up of this combined process for lithium recovery.

Bioleaching of Mine Tailings by Mesophilic: Acidithiobacillus spp., Leptospirillum ferrooxidans, and Thermophilic: Sulfobacillus thermosulfidooxidans Cultures with the Addition of Ag+ Additive

This research focused on applying and comparing the performance of microorganisms with different temperature preferences, assessing the overall percentage recovery of elements (copper, zinc, arsenic, and iron) from mine tailings in the Staré Ransko region (Czech Republic). The study examined the solubilisation process using a mesophilic mixed bacterial culture, including Acidithiobacillus ferrooxidans (AF), Acidithiobacillus thiooxidans (AT), Leptospirillum ferrooxidans (LF), and the thermophilic species Sulfobacillus thermosulfidooxidans (ST). Under biotic conditions, constant process parameters were maintained, including a particle size of 71–100 µm, a pH value of 1.8, agitation at 150 rpm, and a pulp density of 10% (w/v). The only exception was the temperature, which varied for optimal multiplication of cultures (30 °C/50 °C). Additionally, the research examined the impact of AgNO3 additive at a concentration of Ag+ ions of 5 mg·L−1. The research focused on the solubilisation of Cu, Zn, Fe, and As and the results demonstrated that the application of microorganisms ST, combined with the action of Ag+ ions, enhanced the kinetics of the extraction process, leading to the highest final recovery of all elements (Cu 91.93%, Zn 85.67%, As 69.16%, and Fe 71.72%) under the specified conditions. The study observed that the most significant increase in solubilisation can be attributed to the additive cation in the case of copper (AF, AT, LF/Ag+ by 40.33%; ST/Ag+ by 44.39%) and arsenic (AF, AT, LF/Ag+ by 23.79%; ST/Ag+ by 26.08%). Notably, the intensification of leaching using thermophilic bacteria at a constant suspension temperature of 50 °C was primarily determined for Zn (ST by 18.36%, ST/Ag+ by 14.24%). After 24 days of extraction, the emergence of secondary minerals, namely CaSO4·2H2O and KFe3(SO4)2(OH)6, was identified. The study highlighted a significant increase in the extraction mechanism kinetics due to the influence of microorganisms, contrasting with the low solubilities observed under abiotic conditions (Cu 9.00%, Zn 14.17%, As 4.28%, Fe 6.23%).

Bioleaching of sewage sludge for copper extraction using Acidithiobacillus thiooxidans: Optimization and ecological risk assessment

In this study, Acidithiobacillus thiooxidans was used for the bioleaching of copper (Cu) from sewage sludge. In order to find optimization conditions, three factors including solid-to-liquid ratio (S/L) (0.01-0.2 %(w/v)), initial element sulfur (S0) (1–10 g/L), and initial pH (1–3) have been investigated. Based on response surface methodology (RSM) determined a significant reduced quadratic model with a p-value of 0.0022 (<0.05 significant level). The maximum Cu recovery was 85.3% in the optimum condition of S/L = 0.16% (w/v), S0 = 8.2 g/L, and pH = 1.4. Furthermore, a kinetic study based on a shrinking core model was performed and the result showed that chemical reaction was rate limiting in the extraction. Toxicity Characteristic Leaching Procedure (TCLP) results after bioleaching showed the bioleaching process detoxified sludge and the bioleached sludge residue was well within the regulatory limits for disposal. The germination seed with adding bioleached and unbioleached sludge to the soil was determined. Various parameters such as Germination Index (GI), Tolerance Index (TI), Vigor Index (VI), and stem length showed that the sewage sludge indices significantly increased than the sample soil with unbioleached sludge.

Bioleaching of a lateritic ore (Piauí, Brazil) in percolators

Heap leaching of laterites for extraction of nickel and cobalt is an attractive alternative to capital and energy intensive high pressure acid leaching, the dominant hydrometallurgical processing technology for limonitic laterites. Conventional approach for heap leaching of laterites is leaching with sulfuric acid. Consumption of sulfuric acid during heap leaching is substantial and industrial-scale operations require construction of a sulfuric acid production plant on site. In this study, heap bioleaching of laterites was simulated in laboratory scale column percolators and bioleaching of nickel and cobalt from lateritic material was successfully demonstrated for the first time. The process is based on biooxidation of the bacterially modified “wet sulfur” inside column percolators by sulfur-oxidizing acidophilic bacteria Acidithiobacillus thiooxidans. The “wet sulfur“ was generated in a bioreactor with the bacterial culture, harvested, and mixed with lateritic ore before forming agglomerates to be filled in the percolator columns. Liquid was circulated with a flow rate of 8 mL/min. Maximum metal extraction was 66% nickel, 95% cobalt, 10% iron, 55% magnesium and 89% manganese from the Piauí lateritic ore after one month bioleaching. For comparison, chemical leaching with 1 M sulfuric acid with or without addition of 10 g/L of ferrous sulfate heptahydrate as reductant resulted in extraction of approximately 80% nickel, 86% cobalt, 33% iron, 50% magnesium and 81% manganese. With bioleaching a higher cobalt but lower nickel and iron extraction was achieved, i.e. a better selectivity of nickel over iron extraction, as well as a relatively higher pH of the pregnant leach solution requiring less limestone and, consequently, lower CO2 emission and generation of iron cake waste in case of laterite bioleaching. Overall, the results are promising and show potential of laterite heap bioleaching to be further developed to application on industrial scale.

An experimental study of the effects of bacteria on asphaltene adsorption and wettability alteration of dolomite and quartz

The adsorption of asphaltene on the rock surface and the changes in its wettability are very relevant issues in flow assurance and oil recovery studies, and for carbonate reservoirs, they are even more important. During microbial enhanced oil recovery (MEOR) processes, wettability alteration is considered a crucial mechanism leading to improved oil recovery. Therefore, it is essential to understand the mechanisms of surface wettability changes by bacteria and biosurfactants and find new and reliable methods to prevent asphaltene adsorption. Hence, the main aim of this research was to investigate the effect of a mixture of thiobacillus thiooxidans and thiobacillus ferooxidans microorganisms with an optimum effective temperature of around 30 °C (referred to as mesophilic bacteria), as well as a mixture of two moderate thermophiles Sulfobacillus thermosulfidooxidans for operating temperatures around 50 °C (referred to as moderately thermophilic bacteria) on the adsorption of asphaltene samples isolated from two different crude oils onto main reservoir minerals (i.e., quartz and dolomite). The results indicated that after two weeks of mineral aging in moderate thermophilic bacteria, the adsorption of asphaltene on both minerals increased between 180 and 290%. Fourier-transform infrared spectroscopy (FTIR) analysis for quartz and dolomite samples demonstrated that after aging in bacterial solution, bonds related to the adsorption of bacterial cells and biosurfactant production appear, which are the main factors of change in wettability. Alteration in wettability towards hydrophilicity expands hydrogen bonds on the surface, thus improving asphaltene adsorption due to polar interaction. Asphaltene 1 changed the contact angle of dolomite from 53.85° to 90.51° and asphaltene 2 from 53.85° to 100.41°. However, both strains of bacteria caused a strong water-wetting effect on the dolomite rock samples. The influence of moderate thermophilic bacteria on surface wettability is more significant than that of mesophilic bacteria, which may be caused by the high protein content of these bacteria, which expands hydrogen bonding with the surface. Adsorption of asphaltenes on dolomite rocks previously aged with bacteria showed that the wetted rock samples retained their water-wet state. This study highlights the dual impact of the used microorganisms. On one hand, they significantly reduce contact angles and shift wettability towards a strongly water-wet condition, a crucial positive factor for MEOR. On the other hand, these microorganisms can elevate the adsorption of asphaltenes on reservoir rock minerals, posing a potential challenge in the form of formation damage, particularly in low-permeability reservoirs.

Improving heavy metal removal efficiency from steel sludge: Application of the coupled ultrasonic-bioleaching treatment

&lt;p&gt;This study assessed the efficiency of Fe, Al, Ni and Sr removal from the steel sludge using the coupled bioleaching with Thiobacillus thiooxidans and ultrasonic waves. Growth conditions were optimized using the surface response method. The bacterium was adapted successively to three heavy metal-containing solutions with different concentrations of 100, 200, and 300 mg/ml. Samples were exposed to ultrasonic waves at frequencies of 30, 60 and 90 kHz and durations of 20, 30 and 40 min for two weeks. The highest Fe removal efficiency of 98.45% was obtained using the T. thiooxidation, wave frequency of 30 kHz for 40 min, and pulp density of 100 mg/ml. The maximum removal efficiency was found to be 99.74% for Al under a wave frequency of 90 kHz for 20 min and a pulp density of 300 mg/ml, approximately 100% for Ni under a wave frequency of 30 kHz for 20 min and a pulp density of 300 mg/ml, and 98.45% for Sr under a wave frequency of 90 kHz for 20 min and a pulp density of 300 mg/mL. Results showed that the removal efficiency of Ni and Al bioleaching improved significantly (P &amp;lt;0.05) under the ultrasonic irradiation while the removal efficiency of Fe and Sr remained statistically unchanged (P&amp;gt; 0.05) with and without the application of ultrasonic waves.&lt;/p&gt;&#x0D;

Investigating the impact of inoculation on bioleaching of copper flotation concentrate using a continuous reactor at pilot scale - study case: Tizert deposit

The world is witnessing a major challenge of energy transition which involves the decline of fossil fuels specifically, but which increasingly calls on metallic resources, base metals, mainly copper. The main methods of processing copper minerals are pyrometallurgical and hydrometallurgical processes. Biohydrometallurgy appeared as a very interesting and promising alternative way. In this context, the bioleaching process was studied for the treatment and upgrading of the Tizert concentrate from the Tizert deposit, located 160 km southeast of Agadir, Morocco. Chemical, mineralogical and metagenomic characterizations were carried out on samples from the Tizert deposit. Metagenomic analysis of deposit samples revealed the abundance of genera Paenibacillus (11.38 %), followed by Phynylobacterium (7.54 %) when DSM 670 medium was used while Acidithiobacillus (41.48 %) and Pseudomonas (35.01 %) were abundant when DSM 71 medium was used, indicating the need to use selected consortium species for copper bioleaching experiments. Inoculation with the three species Acidithiobacillus ferrooxidans DSM 583, Leptospirillum ferriphilum DSM 14647 and Acidithiobacillus thiooxidans DSM 14887 was carried out. Laboratory scale leaching experiments with and without inoculation with the bacterial consortium were carried out on the copper concentrate obtained by flotation pretreatment, to compare the copper leaching behavior and yield. Inoculation with exogenous bioleaching bacteria improved the copper yield to 91 % compared to the leaching process without inoculation. These results were confirmed by a pilot-scale study with a copper yield of 95 %.

The Role of Bacteria and Factors Affecting the Biodegradation of Pollutants in the Biofilter Reactor: A Review

The findings showed that several types of bacteria can help biodegrade organic pollutants, such as Comamonadacea, which can biodegrade volatile fatty acids and aromatic compounds. Proteobacteria, Bacteroidetes, and Actinobacteria can biodegrade ammonium. Burkholderiales can biodegrade ferric ions and hydrogen. Comamonas testosteroni is able to biodegrade nitrates. Pseudomonas taiwanensis, Acinetobacter guillouiae, and Klebsiella pneumoniae can reduce copper, chromium and zinc levels. Azolla biomass reduces strontium. Rhodospirillum sp. can biodegrade cadmium, mercury, lead, and nickel. Gallionella ferruginea and Leptothrix sp. can biodegrade arsenic and manganese. Gracilaria sp. can biodegrade aluminum, chromium, and zinc. Desulfovibrio sp. can biodegrade copper, zinc, nickel, iron, and arsenic. Thiomonas sp. can biodegrade arsenic and iron. Thauera selenatis can biodegrade copper, zinc, cadmium, nickel, lead, cobalt, chromium, and mercury. Thiobacillus thiooxidans can degrade both zinc and copper. Sargassum filipendula biodegrades copper and nickel. Meanwhile, in the findings of the factors that affected biofiltration, it was identified that there were four that played a significant role such as temperature, dissolved oxygen, hydraulic retention time, organic loading rate, biological organisms, and supply nutrients. In conclusion, several types of bacteria grow and help biodegrade in biofilter reactors. This is inseparable from the supporting factors that increase the efficiency of pollutant reduction in biofilter reactors.

Disorder and amino acid composition in proteins: their potential role in the adaptation of extracellular pilins to the acidic media, where Acidithiobacillus thiooxidans grows.

There are few biophysical studies or structural characterizations of the type IV pilin system of extremophile bacteria, such as the acidophilic Acidithiobacillus thiooxidans. We set out to analyze their pili-comprising proteins, pilins, because these extracellular proteins are in constant interaction with protons of the acidic medium in which At. thiooxidans grows. We used the web server Operon Mapper to analyze and identify the cluster codified by the minor pilin of At. thiooxidans. In addition, we carried an in-silico characterization of such pilins using the VL-XT algorithm of PONDR® server. Our results showed that structural disorder prevails more in pilins of At. thiooxidans than in non-acidophilic bacteria. Further computational characterization showed that the pilins of At. thiooxidans are significantly enriched in hydroxy (serine and threonine) and amide (glutamine and asparagine) residues, and significantly reduced in charged residues (aspartic acid, glutamic acid, arginine and lysine). Similar results were obtained when comparing pilins from other Acidithiobacillus and other acidophilic bacteria from another genus versus neutrophilic bacteria, suggesting that these properties are intrinsic to pilins from acidic environments, most likely by maintaining solubility and stability in harsh conditions. These results give guidelines for the application of extracellular proteins of acidophiles in protein engineering.

Traces of life in Río Tinto (Spain): Decoupling of morphological and geochemical biosignatures

The Río Tinto system located in southern Spain is a 100-km long acidic (pH ≈ 2.3) river rich in dissolved iron, sulfate and heavy metals. The red-tinted river, formed as a product of natural acid rock drainage that has been exacerbated over the years by anthropogenic mining activities, hosts a variety of extremophilic microorganisms that are potential analogues to those found on early Earth and ancient Mars. Here we detailed the investigation of potential biosignatures in the forms of microbialites and sediments deposited along the river. The 20-50 cm thick microbialites (estimated age at most 100 years) are composed of layered structures of Fe(III) minerals with alternating porosities. High resolution imaging and elemental mapping coupled to mineralogical analysis suggest the transformation of K-jarosite (KFe3(SO4)2(OH)6; Vm = 153 cm3/mol) to goethite ( α -FeOOH; Vm = 21 cm3/mol), generating porosities in the process due to a reduction in the molar volume Vm. Meanwhile, riverbed sediments are composed of mixtures of schwertmannite (Fe 8 O8(OH)6(SO4) · nH2O), jarosite, goethite and other minor minerals (ferrihydrite, lepidocrocite, hematite). Culture experiments indicate that crystalline jarosite is formed via transformation from poorly-crystalline schwertmannite (Fe 8 O8(OH)6(SO4) · nH2O), but that the presence of Acidithiobacillus thiooxidans actually inhibits this transformation, possibly due to organic matter stabilization of schwertmannite. Fe isotopic analyses show no clear variations between layers in the microbialites (δ56Fe = -0.85 ± 0.10 ‰), while the riverbed sediments indicate progressively more negative values downstream over a 5-km distance (δ56Fe = +0.4 to -0.8‰). The spatial isotopic pattern of the riverbed sediments is consistent with rapid microbial oxidation of Fe2+ and inconsistent with the much slower abiotic Fe2+ oxidation rate. Taken together, our results show that while the microbialites’ structure is suggestive of microbially-induced precipitation, its geochemical features are not. Meanwhile, the inconspicuous-looking riverbed sediments provide clear evidence of biogenicity from their isotopic compositions. This highlights the challenge in detecting life especially when decoupling of morphological and geochemical biosignatures is to be expected.

An integrated approach of bioleaching-enhanced electrokinetic remediation of heavy metals from municipal waste incineration fly ash using Acidithiobacillus spp

Introduction: Municipal solid waste (MSW) incineration fly ash is a harmful residue formed during the incineration process. It contains high concentrations of hazardous heavy metals, such as lead, zinc, aluminum, and iron.Methodology: In this study, bioleaching integrated with an electrokinetic approach for heavy metal remediation from MSW incineration fly ash using Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans bacteria was tested.Results and discussion: The physicochemical properties of fly ash included a particle size of 26.1 μm, with the presence of heavy metals. A. ferrooxidans and A. thiooxidans produced sulphuric acid (0.0289 M and 0.0352 M) during the proliferation; this acid enhances the bioleaching of heavy metals from fly ash. The results of an integrated approach showed an 85%, 47%, 92%, 85%, 46%, 67% 11%, and 55% removal of the heavy metals K, Na, Ca, Mg, Al, Zn, Pb, and Mg, respectively, in the presence of A. ferrooxidans. Overall, these results evidenced that heavy metals were completely removed from the fly ash using an integrated approach. Therefore, this integrated approach can be used as an effective heavy metal removal method for treating fly ash in MSW.

Bioleaching of Rare Earth Elements: Perspectives from Mineral Characteristics and Microbial Species

Bioleaching exhibits high potential for the processing of low-grade complex mineral resources. With the development of the economy and an increase in demand, rare earth elements (REEs) in secondary resources, such as phosphogypsum, red mud and coal-related resources, are gaining more and more attention. In this review, the bioleaching performance of diverse microorganisms is summarized and compared for primary (mainly monazite) and secondary REE resources, based on publications from the past decade. The mineral characteristics of these REE resources are different, as they can be found in phosphate, sulfate, or silicate forms. Correspondingly, microbial species suitable for use in bioleaching differ. The most efficient bioleaching microbe for monazite is Paecilomyces sp., while Acidianus manzaensis is effective in processing red mud. Acidophilic sulfur oxidizers are suitable for processing acidic phosphogypsum. Acidithiobacillus thiooxidans could recover a significant amount of REEs from coal fly ash. In particular, monazite has a high REE content but extremely low bioleaching efficiency compared to that of secondary resources, supporting the understanding that bioleaching approaches are more competitive for minerals with low REE contents. Overall, great progress has been made over the last decade, as considerable REE recovery rates have been achieved, and the main metabolites of microbes were identified. However, numerous challenges still exist. Future efforts should focus on improving biorecovery efficiency, reducing the cost of cell-culture media, and exploring the interaction mechanism between cells and minerals, with an emphasis on mineralogical phase transformations and the molecular regulation mechanisms inside cells during the bioleaching process.

Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain).

Acidithiobacillus thiooxidans is of paramount importance in the development of biomining technologies. Being widely recognized as an extreme acidophile, extensive research has been dedicated to understanding its significant role in the extraction of several ores in recent years. However, there still exist significant molecular uncertainties surrounding this species. This study focuses on developing a taxonomic assignment method based on the sequencing of the 16S-5S rRNA cluster, along with a qPCR-based technology enabling precise growth determination. Additionally, an approach to understanding its response to acid stress is explored through RT-PCR and MALDI-TOF analysis. Our findings indicate that when subjected to pH levels below 1, the cell inhibits central (carbon fixation and metabolism) and energy (sulfur metabolism) metabolism, as well as chaperone synthesis, suggesting a potential cellular collapse. Nevertheless, the secretion of ammonia is enhanced to raise the environmental pH, while fatty acid synthesis is upregulated to reinforce the cell membrane.

Bioleaching of the α-alumina layer of spent three-way catalysts as a pretreatment for the recovery of platinum group metals

Acid bioleaching of Al by Acidithiobacillus thiooxidans has been explored as an environmentally friendly pretreatment to facilitate the extraction of platinum group metals from spent three-way catalysts (TWC). Biogenic sulfur obtained from desulfurization bioreactors improved the production of acid by A. thiooxidans compared to commercially available elemental sulfur. The lixiviation abilities of bacteria-free biogenic acid and biogenic acid with exponential or stationary phase bacteria were compared against a control batch produced by commercial H2SO4. The maximum Al leaching percentage (54.5%) was achieved using biogenic acids with stationary-phase bacteria at a TWC pulp density of 5% w/v whereas bacteria-free biogenic acid (23.4%), biogenic acid with exponential phase bacteria (21.7%) and commercial H2SO4 (24.7%) showed lower leaching abilities. The effect of different pulp densities of ground TWC (5, 30, and 60% w/v) on Al leaching and bacterial growth was determined. While greater Al leaching yields were obtained at lower TWC pulp density solutions (54.5% at 5% w/v and 2.5% at 60% w/v), higher pulp densities enhanced microbial growth (2.3 × 109 cells/mL at 5% w/v and 9.5 × 1010 cells/mL at 60% w/v). The dissolution of the metal from the solid into the liquid phase triggered the production of biological polymeric substances that were able to absorb traces of both Al (up to 24.80% at 5% w/v) and Pt (up to 0.40% at 60% w/v).

Hibiscus rosa-sinensis as a potential hyperaccumulator in metal contaminated magnesite mine tailings

Mining is one of the major contributors for land degradation and severe heavy metals based soil pollution. In this study, the physicochemical properties of magnesite mine soil was investigated and assess the optimistic and eco-friendly remediation approach with Hibiscus rosa-sinensis with the effect of pre-isolated Acidithiobacillus thiooxidans. The physicochemical properties analysis results revealed that most the parameter were either too less or beyond the permissible limits. The pre-isolated A. thiooxidans showed remarkable multi-metal tolerance up to 800 μg mL−1 concentration of Cr, Cd, Pb, and Mn. Heavy metal content in polluted soil was reduced to avoid more metal toxicity by diluting with fertile control soil as 80:20 and 60:40. The standard greenhouse experiment was performed to evaluate the phytoextraction potential of H. rosa-sinensis under the influence of A. thiooxidans in various treatment groups (G-I to G-V). The outcome of this investigation was declared that the multi-metal tolerant A. thiooxidans from G-III and G-II showed remarkable effect on growth and phytoextraction ability of H. rosa-sinensis on metal polluted magnesite mine soil in 180 d greenhouse study. These results suggested that the combination of H. rosa-sinensis and A. thiooxidans could be used as an excellent hyper-accumulator to extract metal pollution from polluted soil.

Bioleaching of Zinc from Blast Furnace Cast House Dust

Metallurgical dusts are by-products from steel manufacturing. The high iron content of cast house dust (~64%) makes this by-product an interesting iron feedstock alternative. Therefore, its return into the internal steelmaking circuit, specifically in the sinter plant, is a common practice in the steel industry. However, this dust fraction also contains heavy metals, as zinc. As a result of the re-entry of zinc into the process, the zinc concentration in the blast furnace flue gas dust also increases. This prevents the full recirculation of the blast furnace flue gas dust in the steelmaking process despite its relatively high iron content (~35%), thus causing part of the blast furnace flue gas dust to end in the landfill. The goal of this study was to investigate the usage of bacteria, such as the sulfur oxidizing Acidithiobacillus thiooxidans or the iron and sulfur oxidizing Acidithiobacillus ferrooxidans, to leach the undesirable element zinc from the cast house dust while preventing the leaching of iron, by adjusting the sulfur addition and avoiding, at the same time, the accumulation of sulfur in the solid fraction. Experiments proved that a co-culture of A. thiooxidans and A. ferrooxidans can effectively leach zinc from metallurgical dusts, maintaining high iron concentrations in the material. The influence of elemental sulfur on the efficiencies reached was shown, since higher removal efficiencies were achieved with increasing sulfur concentrations. Maximum zinc leaching efficiencies of ~63% (w/w) and an iron enrichment of ~7% (w/w) in the remaining residue were achieved with sulfur concentrations of 15 g/L for cast house gas concentrations of 125 g/L.