The purpose of this study was to investigate the antimicrobial activity of unilamellar nano vesicles (liposome) containing d-limonene against selected fruit rotting fungi (Botrytis cinerea and Penicillium chrysogenum) and food borne illness causing bacteria (Escherichia coli and Listeria monocytogenes). Furthermore, the research evaluated the extended shelf life and enhanced food safety of blueberries treated with d-limonene and liposomes. Liposomal nanoparticles were created by thin lipid film hydration followed by sonication. Mean liposome radius was 100.2±3.1nm. The antibacterial activity against E. coli showed 0.99 and 1.6 log10 reductions in CFUmL−1 at 10μM and 50μM, respectively, within 48h. The log10 reduction was 1.6 at 10μM and 3.4 at 50μM for L. monocytogenes.Germination of B. cinerea conidia was completely suppressed over 48h by a 50μM concentration of limonene. Liposomes exhibited 2.2 and 2.8 log10 reductions for P. chrysogenum, whereas 3.7 and 4.6 log10 reductions were observed for B. cinerea with 10μM and 50μM limonene, respectively. The in vivo study of liposome coatings on blueberries also revealed protection against microbial growth even after nine weeks of storage at 4°C with liposomes reducing blueberry spoilage by more than 60% at the end of nine weeks. The results of this study can benefit the produce industry through both enhancement of food safety and extending the shelf life of blueberries, further highlighting the commercial applications of liposomes.