Thin-layer Chromotography Research Articles

<!DOCTYPE html> <html> <head> </head> <body> Objectives: The study investigated the free radical scavenging and antioxidant potential of the methanol, ethanol and ethyl acetate extracts of turmeric rhizome. Methods: We assessed the phytochemical constituents, total polyphenol and flavonoid content in these turmeric extracts using standard phytochemical analyses. Phytochemical screening of turmeric extracts showed the presence of carbohydrates, proteins, sterols, tannins, flavonoids and saponins. Results: Ethanol extracts showed the highest polyphenol content (71.7 ± 3.0 mg of gallic acid equivalents/g of extract) and flavonoid content (28.5 ± 2.1 mg quercetin equivalents/g of extract) when compared to other extracts of turmeric rhizome. Similarly, ethanol extract of turmeric possessed the higher antioxidant activity (463.25 ± 36.15 μM Fe(II)/g of extract) in FRAP assay. The inhibitory concentration (EC50) of gallic acid, ethanol, methanol and ethyl acetate extract were found to be 27μg, 30μg, 38μg and 47μg respectively in DPPH assay. The H2O2 scavenging activity of the extract (20, 40, 50, 100 and 200g/ml) was increased in a dose dependent manner and ethanol extracts found superior hydrogen peroxide scavenging activity similar to gallic acid standard. The curcuminoids (standard) and the turmeric extracts were separated in Thin layer Chromotography (TLC). The Curcuminoids were separated into curcumin, dimethoxycurcumin, bis demethoxycurcumin with Rf value of 0.91, 0.65 and 0.44 respectively. The composition of turmeric extracts was consistent with the composition of curcuminiods as shown by the Rf values. Conclusion: The study concludes that ethanol extract of turmeric showed high polyphenol and flavonoid content which is attributed to its higher anti-oxidant properties. Key words: Turmeric, Phenolic content, Flavonoids, Ferric reducing antioxidant power assay, DPPH radical scavenging assay. </body> </html>

This study was aimed at investigating the biodistribution and radioimmunoimaging of (131)I-D-D3 in nude mice bearing different types of tumor xenografts. Radioiodination of the D-D3 antibody was performed with the chloramine-T method. The radiochemical purity was determined through thin-layer chromotography. (131)I-D-D3 was injected into healthy Kunming mice via a tail vein, and the %ID/g for various organs was obtained. Similarly, the %ID/g and tumor/nontumor tissue ratio of (131)I-D-D3 in nude mice bearing small cell lung cancer (SCLC) xenografts were obtained. Planar images of (131)I-D-D3 in tumor-bearing nude mice were acquired at different times after injection. The (131)I-D-D3 labeling rate was 86.56% ± 3.8%. The radiochemical purity of (131)I-D-D3 was 99.27% ± 0.6%. After 12 hours of incubation in 37°C water bath, the radiochemical purity was 97.64% ± 0.5% and remained at 88.38% ± 0.4% after 48 hours. After being mixed with healthy human serum for 24 hours, the radiochemical purity was more than 64%. The metabolism of (131)I-D-D3 in healthy Kunming mice was consistent with a two-compartment model with first-order absorption; T(1/2α) and T(1/2β) were 0.25 and 37.89 hours, respectively. The %ID/g of (131)I-D-D3 in SCLC xenografts was much higher than those of other tissues at 48 hours after injection, and the tumor/nontumor tissue ratio also gradually increased with time. After 24 hours of injection, planar imaging was obtained, which clearly showed a contrasting tumor on the right armpit of nude mice bearing SCLC with high concentrations of radioactivity. Also, nude mice bearing gastric cancer showed similar results as that of the SCLC with a lower radioactivity level. No observable accumulation was observed in nude mice bearing pancreatic cancer or lung adenocarcinoma. The labeling rate and radiochemical purity of (131)I-D-D3 were high and stable. (131)I-D-D3 selectively accumulated at tumors that highly expressed progastrin-releasing peptide; therefore, it is a promising radioimmunoimaging reagent for SCLC.

Thin-layer Chromotography Research Articles

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Thin-layer Chromotography Research Articles

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