Eggshell Thickness Research Articles

Effects of rutin on laying performance, egg quality, serum biochemistry, antioxidant capacity and immunity in laying hens fed a diet containing stored soybean meal

This study aimed to investigate the effects of rutin on laying performance, egg quality, serum biochemical parameters, serum immunoglobulin, and antioxidant status in laying hens fed a diet containing stored soybean meal (SBM). The dietary treatments used were arranged in a 2 × 2 factorial arrangement with 2 types of SBM (FSM: SBM was stored in the cold storage warehouses at −20 °C for 45 days and was considered as fresh SBM; RTSM: SBM was stored in room temperature warehouse (15 °C to 25 °C), average temperature was 20 °C for 45 days) and 2 levels of rutin (0 and 500 mg/kg). In total, 384 Hy-Line Brown laying hens (220 days of age) were randomly divided into 4 groups with eight replicates. Pre-feeding period was 1 week, and the experiment lasted for 8 weeks. The results showed that the RTSM diet had no effect on laying performance (p > 0.05). Compared with the diet without rutin, diet with 500 mg/kg rutin exhibited a tendency to increase egg mass compared with the diet without rutin (p = 0.096), and there was a trend of interaction effect on average egg weight (p = 0.061). The RTSM diet decreased the egg Haugh unit significantly compared with the FSM diet at the end of 8th week (p < 0.05). Compared with the diet without rutin, dietary rutin supplementation could affect egg quality as evidenced by increased eggshell thickness, albumen height, egg yolk colour at the end of 4th week and increased eggshell strength and eggshell thickness at the end of 8th week (p < 0.05). Furthermore, dietary rutin supplementation increased urea nitrogen, decreased the immunoglobulin M (IgM) content in serum compared with the diet without rutin (p < 0.05). Compared with the diet without rutin, dietary supplementation with rutin improved the total antioxidant capacity in the serum and liver (p < 0.05). The interactions of SBM × Rutin were observed on egg yolk ratio at the end of 8th week, and the urea nitrogen and IgM content in serum. These findings suggested that diet with RTSM had adverse effects on egg quality in laying hens. The RTSM diet negatively impacted egg quality of laying hens over time when compared to the FSM diet. Dietary rutin supplementation could improve the egg quality, and antioxidant capacity of laying hens.

Effect of rosemary (Rosmarinus officinalis) leaf meal supplementation on production performance and egg quality of laying hens

This experiment was conducted to investigate the supplemental effect of rosemary leaf meal (RLM) on egg production and egg quality in Lohmann-Brown laying hens. For each treatment, six replicates were performed with four 24-week-old laying hens for a total of 96 chickens. Dried rosemary leaf meal (RLM) was present in the commercial laying hen ration at 0, 1.7, 3.5 and 5.2%, respectively. The interactions between dietary treatments, egg storage temperatures (4 °C, 21.5 °C), and egg storage durations (7, 14, 21, 28, and 35 days) were examined in factorial designs using a fully randomized design. The data were analyzed using the Statistical Analysis System (SAS, 2014, Ver. 9.3) and Tukey's multiple range tests to separate the means. The inclusion of dried RLM at a level of 5.2% (p < 0.05) significantly improved the hens' daily egg production (78.9%). Feed conversion ratio (2.25) and egg weight (56.7 g) compared to control treatment. Similarly, at 3.5% and 5.2% RLM supplementation, eggshell weight (5.876 g) and eggshell strength increased significantly (p < 0.05) (compared to control, a weight of 3.76 kg/cm2 and a shell thickness (0.37 mm) was observed. In addition, the groups fed 5.2% RLM had significantly higher albumin weight (34.9 g/L), albumin height (6.9 mm), and yolk weight (15.6 g/L). l), yolk height (15.9 mm), yolk color (5.3 points), and Hough unit (83.9 points) than the control groups. Regarding the external characteristics of stored eggs, a group of hens fed at rates of 3.5 and 5.2% RLM showed significantly greater (p < 0.05) egg weight with increases in storage temperature and duration as a control group. There was a significant interaction effect between stored eggs collected from the supplemented groups in terms of egg weight, weight loss, shell weight, shell strength, and shell thickness stored at specific temperatures and storage durations. There was a decrease in albumen level and Haugh unit (HU) with an increase in storage temperature and duration over treatment (P < 0.05). Therefore, better external quality was observed in eggs collected from a group of chickens fed 4.5 and 5.2% RLM after 35 days of storage compared to a control group. Mean albumen height, albumen weight, HU, yolk height, and yolk color of eggs stored in the refrigerator (4 °C) were an exception for yolk weight, which was higher compared to room temperature (21.5 °C). Significantly low (P < 0.05) albumen height (6.61 to 2.96 cm), Hough unit (82.49 to 47.64 points), and yolk height (14.66 to 12.35 mm) were observed at 35 days storage recorded in the control group. In conclusion, supplementation with RLM at 3.5% and 5.2% improved the performance and quality of both fresh and stored eggs.

The effect of replacing sodium selenite with selenium-chitosan in laying hens on production performance, egg quality, egg selenium concentration, microbial population, immunological response, antioxidant enzymes, and fatty acid composition

The purpose of this study was to investigate into the effects of Se-chitosan and Na selenite supplementation on laying hen production performance, egg quality, egg Se concentration, microbial population, immunological response, antioxidant enzymes activity, and yolk fatty acid profile. Using a 2×2 factorial design, 168 27-wk-old laying hens were randomly divided into 4 treatment groups and 7 replications. Se source (Na selenite and Se-chitosan) and Se level (0.3 and 0.6 mg/kg) were used as treatments. Se-chitosan enhanced egg production percentage and egg mass (P < 0.05) when compared with Na selenite. There was an interaction, with 0.6 mg Se-chitosan/kg causing an increase in albumen height, Haugh unit, albumen index, and shell thickness of fresh eggs (P < 0.05). Se-chitosan increased yolk share, yolk color, and shape index of fresh eggs and shape index, albumen index, albumen height, Haugh unit, yolk color, shell thickness, and specific gravity of stored eggs (P < 0.05). The interaction showed that, 0.6 mg Se-chitosan/kg increased albumen Se concentration and decreased the level of malondialdehyde (MDA) in fresh egg yolk compared with 0.3 and 0.6 mg Na selenite/kg (P < 0.05). When compared with Na selenite, Se-chitosan increased the Se concentration in the yolk and decreased level of MDA in stored egg yolk (P < 0.01). When compared with Na selenite, Se-chitosan reduced coliforms (P < 0.01), increased lactic acid bacteria, and the lactic acid bacteria/coliform ratio (P < 0.05). Se-chitosan supplementation increased antibody response to sheep red blood cells and IgM titers and the activities of glutathione peroxidase and superoxide dismutase in plasma (P < 0.05). Furthermore, compared with Na selenite, supplementing diets with Se-chitosan decreased ∑ n-6 PUFA/∑ n-3 PUFA ratio (P < 0.01). In conclusion, Se-chitosan supplementation of laying hen feed improved production performance, egg quality, egg Se concentration, yolk lipid oxidation, microbial population, immune response, antioxidant enzymes activity, and yolk fatty acid profile, with 0.6 mg Se-chitosan/kg supplementation being optimal.

Lipid profiles and production performance responses of laying hens to dietary Moringa oleifera leaf meal: systematic review and meta-analysis.

The inclusion of Moringa oleifera leaf meal (MLM) in chicken diets especially in developing countries is on the increase due to scarcity of traditional feedstuffs. Therefore, this investigation aimed to explore the effects of MLM on lipid profiles and production characteristics of laying hens. Twenty-three publications retrieved from Web of Science, PubMed, Scopus and Google Scholar search engines were used for the analysis. Data from the 23 studies were analysed using random-effects model in OpenMEE software. Results were presented as standardised mean difference (SMD) at a 95% confidence interval. The results show significant improvement in feed conversion ratio (SMD = - 0.49; p < .001), egg mass (SMD = 0.35; p = .003), Haugh unit (SMD = 0.39; p < .001), eggshell thickness (SMD = 0.63; p < .001) and eggshell weight (SMD = 0.45; p < .001) at a reduced feed intake. On the other hand, egg weight, hen-day egg production and blood high-density lipoprotein cholesterol were not statistically different from controls. Results reveal that dietary MLM enhanced blood cholesterol, low-density lipoprotein (LDL) cholesterol, triglycerides and yolk cholesterol concentrations in laying hens. There is presence of significant heterogeneity and meta-regression revealed that study country, number of hen, housing system, hen age, inclusion level and layer strains were predictors of the treatment effect. In conclusion, the results of this meta-analysis suggest that inclusion of MLM in the diet of laying hens improved feed conversion ratio, aspects of egg quality and blood/yolk cholesterol concentrations in laying hens at a reduced feed intake.

Bioretention filtration prevents acute mortality and reduces chronic toxicity for early life stage coho salmon (Oncorhynchus kisutch) episodically exposed to urban stormwater runoff

As the human population of western North America continues to expand, widespread patterns of urban growth pose increasingly existential threats to certain wild stocks of Pacific salmon and steelhead (Oncorhynchus sp.). Rainfall previously absorbed into the soils of forests and grasslands falls instead on pavement and other hardened surfaces. This creates stormwater runoff that carries toxic metals, oil, and many other contaminants into salmon-bearing habitats. These include freshwater streams where coho salmon (O. kisutch) spawn in gravel beds. Coho salmon embryos develop within a thick eggshell (chorion) for weeks to months before hatching as alevins and ultimately emerging from the gravel as fry. Untreated urban runoff is highly toxic to older coho salmon (freshwater-resident juveniles and adult spawners), but the vulnerability of the earliest life stages remains poorly understood. To address this uncertainty, we fertilized eggs and raised them under an episodic stormwater exposure regimen, using runoff collected from a high-traffic arterial roadway from 15 discrete storm events. We monitored survival and morphological development, as well as molecular markers for contaminant exposure and cardiovascular stress. We also evaluated the benefit of treating runoff with green infrastructure (bioretention filtration) on coho salmon health and survival. Untreated runoff caused subtle sublethal toxicity in pre-hatch embryos with no mortality, followed by high rates of mortality from exposure at hatch. Bioretention filtration removed most measured contaminants (bacteria, dissolved metals, and polycyclic aromatic hydrocarbons), and the treated effluent was considerably less toxic – notably preventing mortality at the alevin stage. Our findings indicate that untreated urban runoff poses an important threat to early life stage coho salmon, in terms of both acute and delayed-in-time mortality. Moreover, while inexpensive management strategies involving bioinfiltration are promising, future green infrastructure effectiveness research should emphasize sublethal metrics for contaminant exposure and adverse health outcomes in salmonids.

The improving effect of soybean isoflavones on ovarian function in older laying hens

Emerging evidence suggests an association between estrogen levels and reduced egg-laying performance as the layer became old. Since soy isoflavones (SF) have estrogen-mimic effects, whether it can enhance production performance and ovarian function of older layers is still not known. A total of 160 Lohmann pink layers (66-wk-old) were used in a 2×2 factorial design, which included 2 egg-laying levels [low (76.89 ± 1.65%; LOW) and normal (84.96 ± 1.01%; NOR)] and 2 different dietary groups [0 mg/kg SF, 20 mg/kg SF] were used. The results showed the NOR group had higher egg-laying rate, egg mass, and feed efficiency during the all phases (P(laying) < 0.05). The unqualified egg rate was lower in NOR group (9-12 wk, 1-12 wk) (P(laying) < 0.05). Dietary supplementation with SF increased the egg-laying rate and feed efficiency (5-8 wk, 9-12 wk, 1-12 wk), increased egg mass (9-12 wk, 1-12 wk) (P(SF) < 0.05). The NOR layers presented higher eggshell quality (redness, yellowness, brightness, eggshell ratio) at 12 wk (P(laying) < 0.05). Eggshell quality was found to be improved by SF (eggshell strength and eggshell thickness), egg albumen quality (higher albumen height and Haugh unit) at 12 wk (P(SF) < 0.05). Supplementing with SF led to an increase in eggshell strength in LOW group (P(laying*SF) < 0.05). The higher serum lever of glucose (GLU) and lower serum lever of follicle stimulating hormone (FSH) were in NOR group (P(laying) < 0.05). Supplementing SF in diets increased serum of estradiol (E2) and insulin-like growth factors-1 (IGF-1), decreased serum of FSH (P(SF) < 0.05). The NOR layers presented lower estrogen receptor α (ERα), estrogen receptor β (ERβ), B lymphoma 2 associated X protein (Bax), cytochrome c (Cytc), interleukin 6 (IL-6), caspase3, caspase9, IKKα, P50, and P65 expression in the ovary (P(laying) < 0.05). Dietary SF supplementation decreased the anti-Müllerian hormone receptor (AMHR), Bax, caspase3, caspase9, Cytc, IL-6, IKKα, P50, P65 expression in the ovary (P(SF) < 0.05). These findings indicated that layers with NOR group had higher production performance, egg quality, and ovarian function, while dietary supplementation with SF improved production performance and ovarian function by reducing inflammation and apoptosis-related genes expression in ovary.

Effects of crossbreeding on growth, production and selected egg quality traits of Improved Horro crosses with Cosmopolitan chickens

The effect of crossbreeding aims to characterize heterosis, additive, and maternal values for growth, egg production and selected egg quality traits in Improved Horro (H), Cosmopolitan (C), ♂Improved Horro*Cosmopolitan♀(HC) and ♂Cosmopolitan*Improved Horro♀(CH) chickens. A completely randomized design was used in the study. A total of 1200 eggs and 300 eggs of each genotype were used for the hatchability and fertility trials. A total of 480 chickens were used for the growth, egg production and quality test. The results demonstrated that egg production traits like egg mass (7.74 ± 0.79%), hen day egg production (5.16 ± 0.24%), hen housed egg production (1.65 ± 0.29%), egg number (1.65 ± 0.06%), and hatchability from fertile eggs set (0.92 ± 0.02%) had positive heterosis. The egg production traits of egg fertility (-0.29 ± 0.01%), age at first egg (-1.77 ± 0.14%), age at peak egg production (-0.44 ± 0.06%), and hatchability from eggs set (-8.20 ± 0.76%) showed negative heterosis. The estimates of heterosis values for the examined egg quality traits were generally positive, with the exception of the yolk weight heterosis estimate value. The estimated direct additive values were positive for all selected egg quality traits except yolk weight, egg weight, and egg width. The estimated direct maternal values were significant and positive for all of the egg quality traits examined, with the exception of yolk weight and eggshell thickness. It was found that CH crossbred had the majority of the traits that were estimated to be associated with higher egg production and quality. HC crossbred was characterized for the majority of the traits linked to growth. Conclusively: the majority of egg traits favor CH crossbred chickens over HC crossbreds, whereas the majority of growth traits favor CH over HC crossbreds.

Effect of cage space allowance on egg production, egg quality, immune responses and anti-oxidant variables in White Leghorn layers

A comprehensive study was conducted to optimise cage space allowance (CSA) for White Leghorn (WL) layers (65-80 weeks of age). For the purpose, a total of 900 layers were housed in California colony cages in an open sided poultry house at three different CSA (422, 563, 844 cm2/bird). Each of the CSA was studied with 20 replicates. The daily egg production (EP), feed intake (FI), feed efficiency (FE, FI/egg mass) and egg quality traits were recorded at the end of each 28d interval. Anti-oxidant responses like lipid peroxidation (LP) and glutathione peroxidase, glutathione reductase and superoxide dismutase in blood were measured at 80 weeks of age. Similarly, the antibody titres against ND vaccine and cell mediated immune response (CMI) against PHA- P were measured at 80 weeks of age. The variation in CSA did not influence EP, egg mass, weight gain and egg shell thickness. The FI reduced and FE improved with reduction in CSA. However, the egg density and egg shell percentage increased with increase in CSA. The LP was higher and the activities of anti-oxidant enzymes were lower at 422 cm2/bird as compared to the higher space allowances. No effect of CSA on ND titre was observed, while the CMI response was higher at 844 cm2/ bird than the other two CSA. It could be concluded that, though the FE was better at 422 cm2/b, the trends of shell quality traits and antioxidant variables indicated 546 cm2/bird as requirement for WL layers in open sided poultry house under tropical regions.

Egg production, egg quality, and fatty acids profiles in eggs and tissues in Lohmann LSL lite hens fed algal oils rich in docosahexaenoic acid (DHA)

Enriching eggs with omega-3 fatty acids (n-3 FA), such as docosahexaenoic acid (DHA), is a well-accepted practice that benefits the egg industry and consumers. However, issues around cost, sustainability, and product acceptance have necessitated the search for alternatives to feeding hens fish oil for DHA enrichment. The effects of feeding 2 algal oils on egg production and DHA enrichment in eggs and selected tissues were investigated. The algal oils were: 1) OmegaPro (OPAO) standardized algal oil for DHA content and 2) Crude algal oil (CAO). A total of 400, 46-wk-old Lohmann LSL lite hens were housed in enriched cages (10 birds/cage) and allocated 5 diets (n=8) for a 12-wk trial. The iso-caloric and -nitrogenous diets were a standard corn and soybean meal diet, standard plus 0.25 or 0.76% OPAO and standard plus 0.23 or 0.69% CAO; algal oils diets supplied similar DHA at each level. Egg production indices (hen day egg production, feed intake, FCR, egg weight, egg mass, and eggshell quality) were monitored for 10 wk. Diet samples were analyzed for fatty acids (FA) on wk 1, 6, and 12 and eggs on wk 4, 5, 6, 9, and 12. At the end of the trial, one hen/cage was weighed and dissected for liver, breast and thigh for FA and long bones for ash content analyses. Concentration of omega-6 to omega-3 FA ratio was 12.9, 6.64, 3.48, 6.96, and 3.59 for standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO, respectively. Algal oils increased (P ≤ 0.046) eggshell thickness linearly. The concentration of DHA in the eggs from the birds fed the standard, 0.23 and 0.76% OPAO, 0.25 and 0.69% CAO was 84, 195, 286, 183, and 297 mg/100g egg, respectively, and algal oils enriched eggs with DHA linearly and quadratically (P ≤ 0.01). In conclusion, algal oils increased the concentration of DHA in eggs and had no adverse effects on egg production and eggshell quality.

PEMANFAATAN TEPUNG DAUN MENGKUDU (Morinda citrifolia) TERHADAP KUALITAS TELUR BURUNG PUYUH

The experiment was conducted to determine influence of additing noni leaf flour on the quality of quail eggs. The materials used were 300 quail eggs 44 weeks old. The experiment used a Completely Randomized Design (CRD) with 4 treatments (0%, 1%, 2% and 3% of noni leaf flour in feed) and 5 replications. Parameters observed were haugh unit and egg shell thickness. The data were analysed using analysis of variance and continued with honest real difference test. The result showed that the addition of noni leaf flour in the feed ration had a significant effect (P&gt;0,01) on the haugh unit and egg shell thickness. The conclusion of this experiment was the addition of noni leaf flour in feed ration had a significant effect on the quality of quail eggs. The P2 treatment (addition of 2% noni leaf flour) in quail feed showed the best results on egg quality that is haugh unit and egg shell thickness.

Dietary exogenous phytase improve egg quality, reproductive hormones, and prolongs the lifetime of the aging Hy-Line brown laying hens fed nonphytate phosphorus

The study examined how adding phytase to nonphytate phosphorus (NPP) diets affected performance, egg quality, reproductive hormones, and plasma biochemical indices in 73- to 80-wk-old laying hens. Six treatments with 5 replicates of 18 Hy-Line brown laying hens each were randomly assigned. Three isonitrogenous, isocaloric diets containing consistent calcium levels (3.8%) were formulated to contain 0.20, 0.25, and 0.30% NPP, treated with or without phytase supplementation (1,000 FYT per kg feed, Ronozyme HiPhos-L, Aspergillus oryzae 6-phytase). The results showed that the addition of phytase to the diet containing 0.20, 0.25, and 0.30% NPP increased egg production by 1.50, 1.64, and 0.97%, respectively, and improved eggshell thickness. Also, use of phytase in the diet contain 0.25, and 0.30% NPP increased the plasma concentration of albumin (ALB), high-density lipoprotein (HDL), phosphorus (P), and plasma follicle-stimulating hormone (FSH), plasma calcium (Ca), estradiol-17β (E2β), and luteinizing hormone (LH). In contrast, the egg weight, feed intake, egg mass, feed conversion ratio, albumen height, Haugh unit, yolk, and shell color were unaffected. It can be advisable to use phytase supplementation in an elderly laying hen's diet contain 0.25, and 0.30% NPP to improve shell quality and positively impact reproductive hormones leading to the persistence of egg production.

The Effect of Dietary Supplementation of Hong Kong Caterpillar (Tenebrio molitor) on Quail Egg Quality

The Hong Kong caterpillar (HC) is an alternative source of animal protein for feed. This research aimed to study the effect of using Hong Kong caterpillars in the quail diet on egg quality. A total of 200 quail aged 8-14 weeks, weighing 110 ± 10 g, were used in the study, with 40% production. This study used a completely randomized design with five treatments and four replications. The laying quail diets were formulated with varying levels of HC, including 0% HC for group A, 3% HC for B, 6% HC for C, 9% HC for D, and 12% HC for E. The egg quality parameters measured were egg yolk fat, egg yolk cholesterol, egg white protein, and eggshell thickness. The results indicated that including 12% HC in the quail diet significantly reduced egg yolk cholesterol and egg yolk fat. However, eggshell thickness and egg white protein remained unaffected. Consequently, it can be concluded that Hong Kong caterpillars can be used in quail diets up to a maximum of 12% to reduce egg yolk cholesterol and fat while maintaining eggshell thickness and egg white protein levels.

Essential Oils as a Dietary Additive for Laying Hens: Performance, Egg Quality, Antioxidant Status, and Intestinal Morphology: A Meta-Analysis

This meta-analysis aimed to evaluate the effects of dietary supplementation with essential oils (EOs) on egg production and quality, antioxidant status in blood serum, and the intestinal morphology of laying hens. The data used were obtained from 38 peer-reviewed publications. The effect size was evaluated by weighted mean differences (WMD) between the experimental treatments (diets added with EOs) and the control treatments (diets without EOs). EO supplementation increased (p &lt; 0.001) egg production (WMD = 2.171%), egg weight (WMD = 0.636 g), egg mass (WMD = 1.679 g/d), and decreased the feed conversion ratio (WMD = −0.074 g/g; p &lt; 0.001). In addition, greater (p &lt; 0.05) eggshell thickness (WMD = 14.262 mm), eggshell strength (0.080 kg/cm2), albumen height (WMD = 0.201 mm), Haugh unit (WMD = 1.102), and yolk color (WMD = 0.071) were observed in response to EO supplementation. In blood serum, the dietary inclusion of EOs increased (p &lt; 0.05) the levels of superoxide dismutase (WMD = 1.147 U/mL), glutathione peroxidase (WMD = 879.553 U/mL), and total antioxidant capacity (WMD = 1.163 U/mL). In the duodenum, jejunum, and ileum, a higher (p &lt; 0.05) villus height (VH), crypt depth (CD), villus width, and VH/CD ratio was observed in response to EO supplementation. In conclusion, the dietary inclusion of essential oils can be used as a nutritional strategy to improve egg production and quality, the antioxidant status of blood serum, and intestinal morphology in laying hens.

Effects of tea residues-fermented feed on production performance, egg quality, antioxidant capacity, caecal microbiota, and ammonia emissions of laying hens.

This study was to assess the effects of tea residues-fermented feed (TR-fermented feed) on production performance, egg quality, serum antioxidant capacity, caecal microbiota, and ammonia emissions of laying hens. A total of 1,296 Lohmann laying hens have randomly distributed four groups with six parallels and fed with diets TR-fermented feed at the rates of 0 (control), 1, 3, and 5%. The inclusion of 1% (TR)-fermented feed resulted in a significant increase in egg-laying rate and average egg weight of birds, and a reduction in the feed-to-egg ratio when compared to the control group (p < 0.05). The addition of 1 and 3% of (TR)-fermented feed significantly improved the Haugh unit of eggs (p < 0.05). The eggshell thickness was observed to increase by almost one-fold upon the inclusion of 3 and 5% (TR)-fermented feed in the basal diet (p < 0.05). The supplementation of 3% (TR)-fermented feed significantly increased the content of methionine, tyrosine, proline, essential amino acids (EAA), alpha linoleic acid (C18:3n6), docosanoic acid (C22:0), docosahexaenoic acid (C22:6n3), twenty-three carbonic acids (C23:0), ditetradecenoic acid (C24:1) and total omega-3 polyunsaturated fatty acids (∑ω-3 PUFA) in the eggs (p < 0.05). The addition of a certain amount of (TR)-fermented feed can enhance the activity of glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) in chicken serum, and reduce the level of malondialdehyde (MDA) (p < 0.05). The ammonia concentration in the hen house of laying hens in the treatment groups decreased significantly (p < 0.05). Bacteroidetes and Firmicutes, the main phyla in the cecal bacterial community, were differentially abundant in each group, comprising greater than 55 and 33%, respectively. Collectively, this research indicates that (TR)-fermented feed supplementation improves the performance of laying hens and reduces ammonia emissions and can be used in industry-scale layer production.

Effect of translucency and eggshell color on broiler breeder egg hatchability and hatch chick weight

A successful hatch has a considerable economic impact on all poultry companies. The aim of the current study was to describe the possible effects of shell translucency (T score) and coloration lightness (L* value) on shell thickness, hatchability, and chick weight. A total of 4,320 eggs from 4 commercial Ross 708 breeder flocks (50-55-wk old) were used. Eggs were selected for T score and L* value. A 3-point subjective scoring system was used for T score (1=low, 2=medium, 3=high), and an electronic colorimeter for L* value, sorting the eggs as light (avg. L*=80.7) or dark (avg. L*=76.0). Data were analyzed using the GLIMMIX procedure of SAS (V9.4) and Tukey's HSD test was performed to separate means, a significant difference was considered when P ≤ 0.05. Results suggest that the color of the eggshell was related to the egg weight on the day of collection (P=0.0056) and at transfer (P=0.0211), in both cases dark eggs were 0.6 g heavier than light eggs. Dark eggs had a 3.8% increased hatchability of egg set (P=0.0481) and yielded 6 µm thicker shells (P=0.0019) when compared to light eggs. Regarding translucency, egg weight at transfer was 0.8 g heavier for T score 1 eggs compared to T score 3 (P=0.0358). The translucency score of 1 had a 6.9% higher hatchability of eggs set (P=0.0127) and 0.7 g heavier chick weight (P=0.0385) compared to T score 3. However, T score 1 eggs had shells 28 µm thinner than the T score 2 and 34 µm thinner than T score 3 (P < 0.0001). An interaction effect was observed for eggshell thickness, L* value, and T score, where eggs classified as light with T score 1 had thinner eggshells compared to those that were dark with T score 3 (P=0.0292). These results suggest that eggshell translucency and coloration lightness can be good noninvasive indicators of eggshell thickness, hatchability, and chick weight in broiler breeder flocks.

Effects of dietary vitamin K3 supplementation on production performance, egg quality, vitamin K-dependent proteins, and antioxidant properties in breeding geese during the laying period

The aim of this work was to investigate the effects of dietary vitamin K3 (VK3) supplementation on production performance, egg quality, vitamin K-dependent proteins, and antioxidant properties in breeding geese during the laying period. A total of one hundred twenty 82-wk-old Wulong geese with similar body weights were randomly divided into 6 groups with 4 replicates and 5 geese each (1 male and 4 female). The geese in the control group were fed a basal diet, and the geese in the treatment groups were fed diets supplemented with different levels of VK3 (2.5, 5.0, 7.5, 10.0, and 12.5 mg/kg) for 11 wk. Dietary VK3 supplementation linearly and quadratically increased feed intake, egg mass, egg weight, and egg production (P < 0.05). Increasing VK3 levels linearly and quadratically increased albumen height, shell thickness and Haugh unit of eggs (P < 0.05). VK3 reduced osteocalcin (OC) and uncarboxylated osteocalcin (ucOC) levels in the serum. Dietary VK3 addition linearly decreased serum malondialdehyde (MDA) levels (P < 0.01). There was linear and quadratic effect in the activity of serum total superoxide dismutase (T-SOD) (P < 0.01), and linear effect in serum total antioxidant capacity (T-AOC) (P < 0.01). In conclusion, dietary VK3 supplementation enhanced the production performance, egg quality, vitamin K-dependent proteins, and antioxidant properties in breeding geese during the laying period. The optimal dose of dietary VK3 supplementation was 10.0 mg/kg.

Effects of oligosaccharides on performance, egg quality, nutrient digestibility, antioxidant status, and immunity of laying hens: a meta-analysis

A meta-analysis was conducted to determine the effects of different types of oligosaccharides (OS) on the egg quality, intestinal profile, nutrient digestibility, and immunity of laying hens. A dataset was constructed from articles published between 2006 and 2023 via a literature search using several keywords related to oligosaccharides and laying hens’ performance. After careful evaluation, the final dataset was developed from 25 in vivo studies comprising 165 comparisons. The meta-regression showed that levels of OS supplementation curvilinearly increased (p < 0.05) hen day egg production (HDEP), and crude protein digestibility (CPD). However, OS supplementation did not affect (p > 0.05) feed intake (FI), feed conversion ratio (FCR), egg mass, egg weight, eggshell thickness, shell strength, and Haugh unit. OS supplementation showed a positively improved antioxidant activity, as indicated by the curvilinear effect on superoxide dismutase (SOD) and malondialdehyde (MDA) concentration. In the categorical meta-analysis, mannan oligosaccharide (MOS), xylooligosaccharides (XOS), and chitosan oligosaccharide (COS) significantly increased (p < 0.01) HDEP and CPD. MOS was the only type of OS that decreased (p < 0.05) FCR while FOS showed to increase (p < 0.01) Haugh unit. To sum up, oligosaccharides could generally increase HDEP but MOS is superior to improving HDEP and FCR while FOS is better than other OS to increase eggshell thickness. HIGHLIGHTS Oligosaccharides increase egg production and protein digestibility in laying hens. Mannan oligosaccharide improves feed conversion ratio in laying hens. Elevated trends of IgG and IgA after supplementation with oligosaccharides. Fructo-oligosaccharides improve eggshell thickness and suppress Malondialdehyde (MDA) in laying hens.

The impact of digestible lysine and sulfur amino acids on eggshell quality and egg weight control in old ISA brown hens during 62 to 74 wk

Modifying dietary amino acids has been proposed as a strategy to improve eggshell quality by slowing down increases in egg weight (EW). This study aimed to investigate the effects of different levels of digestible lysine (dLYS) and ratios of digestible sulfur amino acids (dTSAA) to dLYS on performance and eggshell quality in ISA brown hens. A total of 288 hens were individually housed and assigned to 8 treatments, which combined 2 levels of dLYS (5.9 and 5.5 g/kg) with 4 ratios of dTSAA:dLYS (90, 85, 80, and 75) in a factorial arrangement. The study lasted 12 wk, starting at 62 wk of age. The number of eggs was not affected by the interaction between dLYS and dTSAA:dLYS or their main effect. However, the interaction between dLYS and dTSAA:dLYS showed that reducing the dTSAA:dLYS ratio from 85 to 75 when hens were fed 5.5 g/kg of dLYS resulted in a lower EW. Conversely, when hens were fed 5.9 g/kg of dLYS, no significant difference was found in EW among the different ratios of dTSAA:dLYS. Although there was no interaction between the levels of dLYS and dTSAA:dLYS on eggshell quality, reducing the dLYS level from 5.9 to 5.5 slowed down the deterioration in eggshell-breaking strength and eggshell thickness, regardless of the dTSAA:dLYS ratio. These findings suggest that adjusting dietary dLYS while maintaining the dTSAA:dLYS ratio of no less than 85 may be an effective strategy for decelerating the deterioration of eggshell quality in laying hen operations without impacting the egg production rate.

Methods of In Ovo and Ex Ovo Ostrich Embryo Culture with Observations on the Development and Maturation of the Chorioallantoic Membrane.

Culture of shell-free and windowed eggs for drug testing and other experiments has been perfected for smaller eggs such as those of chickens, where the developing blood vessels of the chorioallantoic membrane (CAM) become accessible for manipulative studies. However, due to the thickness and hardness of the ostrich egg shell, such techniques are not applicable. Using a tork craft mini rotary and a drill bit, we established windowed egg, in-shell-membrane windowed egg, and in-shell-membrane shell-free methods in the ostrich egg, depending on whether the shell membranes were retained or not. Concomitant study of the developing CAM revealed that at embryonic day 16 (E16), the three layers of the CAM were clearly delineated and at E25, the chorionic capillaries had fused with the epithelium while the CAM at E37 had reached maturity and the chorion and the allantois were both 3-4 times thicker and villous cavity (VC) and capillary-covering cells were well delineated. Both intussusceptive and sprouting angiogenesis were found to be the predominant modes of vascular growth in the ostrich CAM. Development and maturation of the ostrich CAM are similar to those of the well-studied chicken egg, albeit its incubation time being twice in duration.

Sweet Potato Peel as Substitute Dietary Energy Source on the Early Laying Performance, Egg Quality Traits and Yolk Biochemical Composition of Japanese Quails (Coturnix coturnix japonica Temminck &amp; Schlegel, 1898)

This study evaluated the potential of sweet potato peels from different varieties as substitute dietary energy in the early laying performance of Japanese quails. A total of 240 heads of ready-to-lay quails were laid out in a Randomized Complete Block Design (RCBD) and were assigned to the following treatments; ACommercial feeds, B-Home-mixed ration with 10% NISC Sp-35, C- Home-mixed ration with 10% NISC Sp-36, and D- Home-mixed ration with 10% Sp Japonita. The data were analyzed using one-way ANOVA for RCBD, and differences were determined using Tukey's Honestly Significant Difference Test (HSD) at p&lt;0.05. The commercial diet improved hen-day egg production, FCR per dozen eggs, FCR per kilogram egg mass, and eggshell thickness. On the other hand, there were no significant differences (p&gt;0.05) observed in egg weight, egg shape index, dry shell weight, yolk-albumen ratio and yolk total antioxidant activity. The yolk color score of HMR diets was significantly higher (p&gt;0.05) and the yolk cholesterol deposition was lesser than those in the commercial diet. The financial analysis showed that the gross return was highest in the commercial ration. However, HMR diets performed better in terms of net income, return over feed cost, egg-to-feed price ratio, benefit-cost ratio, and return over investment.