THg Concentrations In Fish Research Articles

Methylmercury levels and bioaccumulation in the aquatic food web of a highly mercury-contaminated reservoir

The low Ebro River basin (NE Spain) represents a particular case of chronic and long-term mercury pollution due to the presence of an industrial waste (up to 436 μg/g of Hg) coming from a chlor-alkali plant Albeit high total mercury (THg) levels have been previously described in several aquatic species from the surveyed area, methylmercury (MeHg) values in fish individuals have never been reported. Accordingly, in order to investigate bioaccumulation patterns at different levels of the aquatic food web of such polluted area, crayfish and various fish species, were analysed for THg and MeHg content. At the hot spot, THg mean values of crayfish muscle tissue and hepatopancreas were 10 and 15 times, respectively, greater than the local background level. Higher mean THg concentrations were detected in piscivorous (THg = 0.848 ± 0.476 μg/g wet weight (ww); MeHg = 0.672 ± 0.364 μg/g ww) than in non-piscivorous fish (THg = 0.305 ± 0.163 μg/g ww; MeHg = 0.278 ± 0.239 μg/g ww). Although these results indicated that THg in fish increased significantly with increasing trophic position, the percentage of the methylated form of Hg was not strongly influenced by differences in relative trophic position. This is an important finding, since the fraction of THg as MeHg in the top fish predator was unexpectedly lower than for other species of the aquatic food chain. Moreover, mean THg concentrations in piscivorous fish exceed the maximum level recommended for human consumption. From our findings, it is clear that for this specific polluted system, speciation becomes almost mandatory when risk assessment is based on MeHg, since single measurements of THg are inadequate and could lead to an over- or under-estimation of contamination levels.

Mercury species of sediment and fish in freshwater fish ponds around the Pearl River Delta, PR China: Human health risk assessment

This study investigated total mercury (THg) and methylmercury (MeHg) concentrations in five species of freshwater fish and their associated fish pond sediments collected from 18 freshwater fish ponds around the Pearl River Delta (PRD). The concentrations of THg and MeHg in fish pond surface sediments were 33.1–386 ng g −1 dry wt and 0.18–1.25 ng g −1 dry wt, respectively. The age of ponds affected the surface sediment MeHg concentration. The vertical distribution of MeHg in sediment cores showed that MeHg concentrations decreased with increasing depth in the top 10 cm. In addition, a significant correlation was observed between %MeHg and DNA from Desulfovibrionacaea or Desulfobulbus ( p < 0.05) in sediment cores. Concentrations of THg and MeHg in fish muscles ranged from 7.43–76.7 to 5.93–76.1 ng g −1 wet wt, respectively, with significant linear relationships ( r = 0.97, p < 0.01, n = 122) observed between THg and MeHg levels in fish. A significant correlation between THg concentrations in fish (herbivorous: r = 0.71, p < 0.05, n = 7; carnivorous: r = 0.77, p < 0.05, n = 11) and corresponding sediments was also obtained. Risk assessment indicated that the consumption of largemouth bass and mandarin fish would result in higher estimated daily intakes (EDIs) of MeHg than reference dose (RfD) for both adults and children.

Agricultural Wetlands as Potential Hotspots for Mercury Bioaccumulation: Experimental Evidence Using Caged Fish

Wetlands provide numerous ecosystem services, but also can be sources of methylmercury (MeHg) production and export. Rice agricultural wetlands in particular may be important sites for MeHg bioaccumulation due to their worldwide ubiquity, periodic flooding schedules, and high use by wildlife. We assessed MeHg bioaccumulation within agricultural and perennial wetlands common to California's Central Valley during summer, when the majority of wetland habitats are shallowly flooded rice fields. We introduced caged western mosquitofish (Gambusia affinis) within white rice (Oryza sativa), wild rice (Zizania palustris), and permanent wetlands at water inlets, centers, and outlets. Total mercury (THg) concentrations and body burdens in caged mosquitofish increased rapidly, exceeding baseline values at introduction by 135% to 1197% and 29% to 1566% among sites, respectively, after only 60 days. Mercury bioaccumulation in caged mosquitofish was greater in rice fields than in permanent wetlands, with THg concentrations at wetland outlets increasing by 12.1, 5.8, and 2.9 times over initial concentrations in white rice, wild rice, and permanent wetlands, respectively. In fact, mosquitofish caged at white rice outlets accumulated 721 ng Hg/fish in just 60 days. Mercury in wild mosquitofish and Mississippi silversides (Menidia audens) concurrently sampled at wetland outlets also were greater in white rice and wild rice than permanent wetlands. Within wetlands, THg concentrations and body burdens of both caged and wild fish increased from water inlets to outlets in white rice fields, and tended to not vary among sites in permanent wetlands. Fish THg concentrations in agricultural wetlands were high, exceeding 0.2 microg/g ww in 82% of caged fish and 59% of wild fish. Our results indicate that shallowly flooded rice fields are potential hotspots for MeHg bioaccumulation and, due to their global prevalence, suggest that agricultural wetlands may be important contributors to MeHg contamination.

Food web structure and mercury transfer in two contrasting Ugandan highland crater lakes (East Africa)

AbstractVolcanic crater lakes scattered throughout western Uganda are important local sources of water and fish. Two representative but contrasting crater lakes near the Kibale National Park were sampled in 2000; the hyper‐eutrophic Lake Saka, which is highly affected by agricultural practices, and the mesotrophic Lake Nkuruba that is still surrounded by intact forest. The food web structures in these two lakes were assessed using stable nitrogen (δ15N) and carbon (δ13C) isotope analyses, and the mercury (THg) transfer patterns were quantified. The δ15N results indicate that food webs in both lakes are abbreviated, with only one to two trophic levels from primary consumers. The Lake Saka biota had distinctively enriched δ13C values compared with those in Lake Nkuruba, which may be due to 12C‐limited phytoplankton blooms in this lake. In Lake Nkuruba, two introduced tilapiine species and the introduced guppy Poecilia reticulata fed predominantly upon invertebrates and decomposed terrestrial plant material. In Lake Saka, the introduced Nile perch Lates niloticus appeared to occupy the top trophic position, but stable isotope values of the endemic haplochromine cichlids exclude those as Nile perch prey items. THg was found to biomagnify through the food web, reaching highest concentrations in P. reticulata in Nkuruba, which tended to be higher than for L. niloticus in Saka, suggesting increased bioavailability of THg in Nkuruba. Maximum THg concentrations in fish never approached WHO recommended guidelines (200 ng g−1) designed to protect at‐risk groups.

Biomagnification of mercury in fish from Thruston Bay, Napoleon Gulf, Lake Victoria (East Africa)

Total mercury concentrations (THg) were measured in fish from Thruston Bay, Napoleon Gulf in northern Lake Victoria between 1998 and 2000. Total Hg concentrations in Lates niloticus (Nile perch) and Oreochromis niloticus (Nile tilapia) ranged from 10.6 to 77.5ng g–1 and from 15.0 to 44.5ng g–1 wet weight respectively. These concentrations are lower than in the same fish species from elsewhere in Napoleon Gulf and are in the middle of the range of THg concentrations from across Lake Victoria. The rate of THg biomagnification, as indicated by the regression slope of log-THg vs stable nitrogen isotope values (0.28), is within the ranges of biomagnification rates observed in temperate and tropical lakes, suggesting that THg is biomagnifying at a similar rate in Thruston Bay as elsewhere. The low THg concentrations in fish were attributed to the storage capacity, high oxygen concentrations and high organic matter content of the wetlands surrounding Thruston Bay. However, caution is required because the storage capacity and the methylation rates present in the wetlands of Thruston Bay are unknown and the gradual accumulation of THg contamination from other sources (e.g. atmospheric THg) may result in unexpected THg increases in the biota of this system.

Methylmercury and total mercury in estuarine organisms from Rio de Janeiro, Brazil.

Guanabara Bay (GB), located in the Rio de Janeiro State, is still a productive estuary on the south-eastern Brazilian coast. It is an ecosystem heavily impacted by organic matter, oil and a number of other toxic compounds, including Hg. The present study aimed to comparatively evaluate the aquatic total mercury (THg) and MeHg contamination, and the ratios of MeHg to THg (% MeHg), in 3 species of marine organisms, Micropogonias furnieri-carnivorous fish (N = 81), Mugil spp.--detritivorous fish (N = 20) and Perna perna--filter-feeding bivalves (N = 190), which are widely consumed by the population. A total of 291 specimens were collected at the bay in different periods between 1988 and 1998. THg concentrations were determined by cold vapour AAS with stannous chloride as a reducing agent. MeHg was extracted by dithizone-benzene and measured by GC-ECD. Analytical quality was checked through certified standards. All organisms presented both low THg and MeHg concentrations and they were below the maximum limit of 1,000 micrograms Hg.kg-1 wet wt. as established for human intake of predatory fish by the new Brazilian legislation. Carnivorous fish showed higher THg and MeHg concentrations, and also % MeHg in muscle tissues, than organisms with other feeding habits and lower trophic levels. The average of THg concentrations in carnivorous fish was 108.9 +/- 58.6 micrograms.kg-1 wet wt. (N = 61) in 1990 and 199.5 +/- 116.2 micrograms.kg-1 wet wt. (N = 20) in 1998, but they presented different total length and body weights. The average THg content in detritivorous fish was 15.4 +/- 5.8 micrograms.kg-1 wet wt., whereas THg concentrations ranged from 4.1 to 53.5 micrograms.kg-1 wet wt. for the molluscs. The THg and MeHg contents of mussel varied according to the sampling point and water quality. MeHg concentration in detritivorous fish was similar to MeHg concentration in molluscs, but there was a significant difference in the MeHg/THg ratio: the carnivorous fish presented higher MeHg percentages (98%) than the detritivorous fish (54%) and the molluscs (33%). Weight-normalised average concentration of THg in carnivorous fish collected in 1990 (0.18 +/- 0.08 microgram.g-1/0.7 kg wet wt.) and in 1998 (0.16 +/- 0.09 microgram.g-1/0.7 kg wet wt.) presented no significant difference (t = 1.34; P < 0.5). In conclusion, the low THg and MeHg concentrations in the organisms from the GB ecosystem, are related to its eutrophic conditions and elevated amounts of suspended matter. In this situation, Hg could be strongly complexed or adsorbed by the particulate, which would dilute the Hg inputs and reduce its residence time in the water column, with a consequent decrease in its availability to organisms.