Theta Rhythm Research Articles

EEG correlates of self-recognition in morphed faces: association with social anxiety

Recognizing one’s own face is important for self-identification and is considered an indicator of self-consciousness. Social anxiety is related to special attention to self. The aim was to investigate the oscillatory dynamics associated with self-recognition/non-self-recognition in morphed faces and the correlation with social anxiety in these processes. During EEG recordings with 128 electrodes, 48 volunteers (31 females) recognized themselves in morphed faces. During self-recognition, a greater increase in theta rhythm was revealed in the time interval from 800 to 1500 ms than in the non-self-recognition condition. Based on the data on the relationship of the theta rhythm with attention and memory, it could be assumed that the increase in theta rhythm may be related to memory and attention processes when perceiving details, mismatches, and misrepresentation of one’s own face. Social anxiety was positively related to the magnitude of theta rhythm during self-recognition, it could be related to the increased attention that socially anxious people focus on themselves and distortions of their own face.

Cell-type specific contributions to theta-gamma coupled rhythms in the hippocampus

Abstract Distinct inhibitory cell types participate in cognitively relevant nested brain rhythms, and particular changes in such rhythms are known to occur in disease states. Specifically, the co-expression of theta and gamma rhythms in the hippocampus is believed to represent a general coding scheme, but cellular-based generation mechanisms for these coupled rhythms are currently unclear. We develop a population rate model of the CA1 hippocampus that encompasses circuits of three inhibitory cell types (bistratified cells, parvalbumin (PV)-expressing and cholecystokinin (CCK)-expressing basket cells) and pyramidal cells to examine this. We constrain parameters and perform numerical and theoretical analyses. The theory, in combination with the numerical explorations, predicts circuit motifs and specific cell-type mechanisms that are essential for the co-existence of theta and gamma oscillations. We find that CCK-expressing basket cells initiate the coupled rhythms and regularize theta, and PV-expressing basket cells enhance both theta and gamma rhythms. Pyramidal and bistratified cells govern the generation of theta rhythms, and PV-expressing basket and pyramidal cells play dominant roles in controlling theta frequencies. Our circuit motifs for theta-gamma coupled rhythm generation could be applicable to other brain regions.

Community-based reconstruction and simulation of a full-scale model of the rat hippocampus CA1 region.

The CA1 region of the hippocampus is one of the most studied regions of the rodent brain, thought to play an important role in cognitive functions such as memory and spatial navigation. Despite a wealth of experimental data on its structure and function, it has been challenging to integrate information obtained from diverse experimental approaches. To address this challenge, we present a community-based, full-scale in silico model of the rat CA1 that integrates a broad range of experimental data, from synapse to network, including the reconstruction of its principal afferents, the Schaffer collaterals, and a model of the effects that acetylcholine has on the system. We tested and validated each model component and the final network model, and made input data, assumptions, and strategies explicit and transparent. The unique flexibility of the model allows scientists to potentially address a range of scientific questions. In this article, we describe the methods used to set up simulations to reproduce in vitro and in vivo experiments. Among several applications in the article, we focus on theta rhythm, a prominent hippocampal oscillation associated with various behavioral correlates and use our computer model to reproduce experimental findings. Finally, we make data, code, and model available through the hippocampushub.eu portal, which also provides an extensive set of analyses of the model and a user-friendly interface to facilitate adoption and usage. This community-based model represents a valuable tool for integrating diverse experimental data and provides a foundation for further research into the complex workings of the hippocampal CA1 region.

Effects of Frontal-Midline Theta Neurofeedback with Different Training Directions on Goal-Directed Attentional Control.

As a significant component of executive function, goal-directed attentional control is crucial for cognitive processing and is closely linked to frontal-midline theta (FMT) rhythms. However, how up-regulation and down-regulation of FMT through neurofeedback training (NFT) impact goal-directed attention control remains unclear, especially for both short-term and long-lasting effects. Therefore, this study employed a single-blind sham-controlled between-subject design to answer this question. Forty-seven healthy adults were randomly assigned to the up-regulation, down-regulation, or sham control groups. Each group underwent one NFT session per day at the Fz electrode site for four consecutive days. All participants completed a visual search task before, immediately after the first, after the final, and one week following the last NFT session. The down-regulation group significantly reduced FMT activity during NFT and in the resting state (p < = 0.038), while the up-regulation group only showed an upward trend during the training phase (r = 0.721, p = 0.002). The behavioral performance showed no significant improvement in any group (p > 0.05). Importantly, the FMT learning efficacy in the up-regulation group revealed a significantly negative correlation with the change in switch cost (r = -0.602, p = 0.046). These findings suggest a close link between the up-regulation efficacy of FMT rhythms and goal-directed attentional control. In educational or clinical settings, it would be desirable to improve goal-directed attention through enhancement of FMT up-regulation efficacy of NFT in future work.

From oxygen shortage to neurocognitive challenges: behavioral patterns and imaging insights

Environmental hypoxia, resulting from reduced oxygen supply, poses a significant risk of dysfunctioning and damaging the neurocognitive system, particularly in relation to anxiety and stress. Inadequate oxygenation can lead to acute and chronic brain damage. Scholars used behavioral, hemodynamic, and electromagnetic neurofunctional techniques to investigate the effects of normobaric and hypobaric hypoxia on neurocognitive systems. They found a correlation between hypoxia, altered psychomotor responses, and changes in EEG alpha, theta, beta, and gamma rhythms, which affect spatial attention and memory. Hypoxia affects event related potential (ERP) components differently depending on latency. Perceptual responses N1 and P2 remain largely unaffected, while the amplitudes of preattentive MMN, vMMN, and P3a are significantly altered. Late latency components related to attention, particularly P3b, are also altered. These changes illustrate the spectrum from sensory detection to more complex cognitive processing, highlighting the brain's efficiency in managing information. Interestingly, the amplitudes of P3b, ADAN and CNV can increase with increased cognitive demands in hypoxia. This suggests a compensatory response. Prolonged exposure exacerbates these effects, resulting in compensatory delayed behavioral responses and alterations in behavioral monitoring and conflict inhibitory control, as reflected by reduced amplitudes in some attention related ERP components, including N2, N2pc, and ERN. Thus, neurocognitive function and integrity are under stress. ERP sources and hemodynamic images reveal that vulnerable brain regions include the frontal prefrontal cortices, hippocampus, basal ganglia, and parietal and visual cortices, which are essential for attention related processes like decision making and spatial memory. The auditory system appears less affected.

Input / Output Relationships for the Primary Hippocampal Circuit.

The hippocampus is the most studied brain region but little is known about signal throughput -- the simplest, yet most essential of circuit operations -- across its multiple stages from perforant path input to CA1 output. Using hippocampal slices derived from male mice, we have found that single-pulse lateral perforant path (LPP) stimulation produces a two-part CA1 response generated by LPP projections to CA3 ('direct path') and the dentate gyrus ('indirect path'). The latter, indirect path was far more potent in driving CA1 but did so only after a lengthy delay. Rather than operating as expected from the much discussed trisynaptic circuit argument, the indirect path used the massive CA3 recurrent collateral system to trigger a high frequency sequence of fEPSPs and spikes. The latter events promoted reliable signal transfer to CA1 but the mobilization time for the stereotyped, CA3 response resulted in surprisingly slow throughput. The circuit transmitted theta (5Hz) but not gamma (50Hz) frequency input, thus acting as a low-pass filter. It reliably transmitted short bursts of gamma input separated by the period of theta wave - CA1 spiking output under these conditions closely resembled the input signal. In all, the primary hippocampal circuit does not behave as a linear, three-part system but instead uses novel filtering and amplification steps to shape throughput and restrict effective input to select patterns. We suggest that the operations described here constitute a default mode for processing cortical inputs with other types of functions being enabled by projections from outside the extended hippocampus.Significance statement Despite intense interest in hippocampal contributions to behavior, surprisingly little is known about how signals are processed across the network linking cortical input to CA1 output. Here, we describe the first input/output relationship for the system with results challenging the traditional tri-synaptic circuit concept. Signal throughput requires mobilization of recurrent activity within CA3 to amplify sparse input from the dentate gyrus into an unexpectedly stereotyped composite response. Potent low-pass filters determine effective input patterns. These results open the way to new analyses of how variables such as aging affect hippocampus and its contributions to behavior while providing material needed for biologically realistic models of the structure.

Neuralink: Advancing Brain-Machine Interfaces for Enhanced Human-Machine Interaction

Using a very modern, cutting-edge brain-machine interface technology, Neuralink could be the untold game-changer of the art of human interaction with technology, in that it should create direct communication paths between the brain and external devices. The implant is placed into the motor cortex of the brain; it can pick up and will send delta, theta, and gamma waves from the brain via a Bluetooth transmitter. This paper discusses, in detail, the technology behind the N1 device, including implantation into the brain and how data from the neural network can be transmitted. Beyond found technology, we go ahead to discuss new application examples of Neuralink with paralyzed people that will show how it could help them resume movement or the control of mechanical devices such as an exoskeleton, cars, or home appliances. Potential benefits may involve enhanced therapy, mobility, and human enhancement. The paper discusses several of the ethical and social issues that this type of technology raises, including privacy, access, and regulatory issues. As Neuralink continues and presses forward brain-machine interfaces, it has the transformative potential to change healthcare delivery, human abilities, and how we interface in our world.

The Comparison of Two Multitasking Approaches to Cognitive Training in Patients after Coronary Bypass Surgery: Theta Activity Changes and &lt;i&gt;sLORETA&lt;/i&gt; Analysis Data

The study investigated the changes in theta activity and localization of its sources by standardized low resolution brain electromagnetic tomography (sLORETA) in patients who have underwent two variants of multitasking cognitive training (CT) in the early postoperative period of coronary artery bypass grafting (CABG). Two groups were formed in a pseudo-random way, which differed according to the type of motor problem used: CT I (n = 27) – a postural balance task and CT II (n = 27) – a simple visual-motor reaction. Cognitive tasks were the same for both groups (counting backwards, verbal fluency, and unusual uses for common objects). Daily sessions of CT were held from the 3rd to 4th day after CABG, with a duration of 5 minutes on the 1st day of training and up to 20 minutes on the 6th to 7th day of training. The current density of theta rhythm sources was lower before CABG than after surgery in the CT II group only. The most significant differences are in the Brodmann area 31, the parietal occipital lobes and precuneus, which may indicate damage associated with cardiac surgery. This effect was not observed in the CT I group. The results of our study demonstrated the informativeness of sLORETA indicators to determine an effective cognitive recovery option after CABG. The reduction of the severity of damaging effects of CABG during training using cognitive tasks and postural balance task was shown. Further research is needed to determine the optimal mode and duration of cognitive training to maximize the functional reserves of such patients.

Motor-related oscillations reveal the involvement of sensorimotor processes during recognition memory

Certain object properties may render an item as more memorable than others. One such property is manipulability, or the extent to which an object can be interacted with using our hands. This study sought to determine if the manipulability of an item modulates memory task performance on both a behavioural and neural level. We recorded electroencephalography (EEG) from a large sample of right-handed individuals (N = 53) during a visual item recognition memory task. The task contained stimuli of both high and low manipulability. Analysis focused on activity in the theta rhythm (3.5–7 Hz), which has been implicated in sensorimotor integration, and the mu rhythm (8–14 Hz), the primary oscillation associated with sensorimotor related behaviours. At both encoding and retrieval, theta oscillations were greater over the left motor region for high manipulability stimuli, suggesting that an item’s sensorimotor properties are assessed immediately upon presentation. Manipulability did not affect activity in the mu rhythm. However, mu oscillations over the left motor region were lower during the retrieval of old versus new items and response time was faster for old items, aligning with the cortical reinstatement hypothesis. These results collectively reveal an association between motor oscillations and memory processes, highlight the involvement of sensorimotor processing at both encoding and retrieval.

Isolated theta waves originating from the midline thalamus trigger memory reactivation during NREM sleep in mice

During non-rapid eye movement (NREM) sleep, neural ensembles in the entorhinal-hippocampal circuit responsible for encoding recent memories undergo reactivation to facilitate the process of memory consolidation. This reactivation is widely acknowledged as pivotal for the formation of stable memory and its impairment is closely associated with memory dysfunction. To date, the neural mechanisms driving the reactivation of neural ensembles during NREM sleep remain poorly understood. Here, we show that the neural ensembles in the medial entorhinal cortex (MEC) that encode spatial experiences exhibit reactivation during NREM sleep. Notably, this reactivation consistently coincides with isolated theta waves. In addition, we found that the nucleus reuniens (RE) in the midline thalamus exhibits typical theta waves during NREM sleep, which are highly synchronized with those occurring in the MEC in male mice. Closed-loop optogenetic inhibition of the RE-MEC pathway specifically suppressed these isolated theta waves, resulting in impaired reactivation and compromised memory consolidation following a spatial memory task in male mice. The findings suggest that theta waves originating from the ventral midline thalamus play a role in initiating memory reactivation and consolidation during sleep.

Integration of rate and phase codes by hippocampal cell-assemblies supports flexible encoding of spatiotemporal context.

Spatial information is encoded by location-dependent hippocampal place cell firing rates and sub-second, rhythmic entrainment of spike times. These rate and temporal codes have primarily been characterized in low-dimensional environments under limited cognitive demands; but how is coding configured in complex environments when individual place cells signal several locations, individual locations contribute to multiple routes and functional demands vary? Quantifying CA1 population dynamics of male rats during a decision-making task, here we show that the phase of individual place cells' spikes relative to the local theta rhythm shifts to differentiate activity in different place fields. Theta phase coding also disambiguates repeated visits to the same location during different routes, particularly preceding spatial decisions. Using unsupervised detection of cell assemblies alongside theoretical simulation, we show that integrating rate and phase coding mechanisms dynamically recruits units to different assemblies, generating spiking sequences that disambiguate episodes of experience and multiplexing spatial information with cognitive context.

Decreasing brain activity caused by acute administration of ketamine and alcohol - A randomized, controlled, observer-blinded experimental study.

Substance abuse is a major public health problem. In recent years, ketamine, which is a parenteral anesthetic, has been consumed increasingly as an illicit drug together with alcohol, although little is known of how this association alters brain activity. The present study investigated the influence of progressive doses of ketamine, associated with alcohol, on electrophysiological activity. For this, 72 late-adolescent (8-10-week-old) male Wistar rats received either ketamine only, at low (10mg/kg), intermediate (20mg/kg) or high (30mg/kg) doses via intraperitoneal injection, or alcohol (2mL/100g) via oral gavage followed by ketamine (at low, intermediate, and high doses). Electroencephalograms (EEG) and electromyographic recordings were obtained 5min after the final application of the drug. When administered alone, ketamine resulted in an increase in delta, theta, beta, and gamma brainwaves, with a more pronounced effect being detected at the highest dose (30mg/kg) in the case of the delta, beta, and gamma waves. The amplitude of the alpha brainwaves was reduced at all doses of ketamine, but less intensively at the highest dose. When administered alone, alcohol reduced all the brainwaves, with the reduction in the alpha waves being exacerbated by ketamine at all doses, and that of the theta and beta waves being boosted at the lowest dose. The intermediate dose of ketamine (20mg/kg) reverted the alcohol-induced reduction in the theta and gamma waves, whereas the high dose increased delta, theta, beta, and gamma bandpower. Overall, then, while ketamine enhances the depressant effects of alcohol on the alpha brainwave at all doses, a low dose intensified this effect on the theta and beta 175 waves, whereas a high dose produces neuronal hyperexcitability in the theta and 176 gamma bandpower.

Comparative exploration on EEG signal filtering using window control methods

Electroencephalography (EEG) is used to monitor brain activity. The brain signals consist of different frequency band signals delta, theta, alpha, beta, and gamma waves. The signals are affected by external noise which reduces the quality of the EEG signal due to which it becomes difficult to do further processing of EEG signals like feature extraction or extraction of meaningful features from EEG signal. Therefore, it becomes important to filter the noise from the EEG signal before feature extraction or classification of the EEG signal. The research article presents an overview of different types of windowing filter techniques like Rectangular, Bartlett, Hamming, Hanning, and Kaiser windows applied for finite impulse response (FIR) behavior which is used for EEG signal processing for different brain waves processed in different frequency bands. The comparative analysis is carried out in terms of the response time of brain frequency bands for different windowing filter techniques using the MATLAB 2023 signal processing simulation tool. The novelty of the work lies in estimating minimum latency and appropriate filter selection for various typical EEG waves, since the EEG signals are pre-supposed in the hardware chip design, noise elimination is the first step in high-performance computing applications. The Bartlett window band stop has an optimal response time of 12.666 s for delta waves, a highpass filter with a response time of 16.187 s for theta waves, a bandpass with a response time of 13.122 s for alpha waves, a highpass filter with a response time of 17.866 s for beta waves, and a highpass filter with a response time of 13.797 s for gamma waves. The Barlett window FIR filter is well-suited for EEG applications.

Modulation of Brain Activities in Healthy Individuals by Acupuncture at Quchi (LI11).

This research investigated the modulation of acupuncture at Quchi (LI11) on the brain activities in healthy individuals. Sub-bands power and EEG microstate analysis were carried out at pre-acupuncture, acupuncture, needle retaining and post-acupuncture periods in both the acupuncture group (n = 16) and control group (n = 18). Four microstate classes (A-D) were derived from the clustering procedure. Regression analysis was conducted, together with a two-way repeated measures ANOVA, which was then followed by Bonferroni correction. In the acupuncture group, we found the beta power during the acupuncture periods was significantly reduced. The channel-by-channel analysis revealed that acupuncture at LI11 mainly altered the power of delta, theta, and alpha waves in specific brain regions. The delta power increased predominantly in parietal, occipital, and central lobes, while theta and alpha power decreased predominantly in temporal, frontal, parietal, and occipital lobes. During the acupuncture period, participants in the acupuncture group showed a significant increase in both duration and contribution of microstate A, as well as the bidirectional transition probabilities A and B/D. Microstate analysis showed that acupuncture at LI11 significantly enhances the activity of microstate A and potentially strengthens the functional connectivity between the auditory network and either the visual network or the dorsal attention network. These correlational results indicate that acupuncture at LI11 mainly affects activities of the frontal, temporal, parietal, and occipital lobes. These findings highlight the potential of microstate as neuroimaging evidence and a specific index for elucidating the neuromodulatory effects of acupuncture at LI11.

Camphor alters occipital electrocorticographic patterns during sleep deprivation in Wistar rats

IntroductionSleep disorders are common in the general population, necessitating the search for new strategies to address this public health challenge. The study aims to describe the electrocorticographic and behavioral changes in sleep deprived Wistar rats exposed to varying doses of camphor, to assess its effects on sleep and its potential as a sleep-inducing drug.Materials and MethodsFor the electrocorticographic evaluation, seventy-two rats were randomly assigned to distinct groups: a control group, a sleep-deprived group, three sleep-deprived groups receiving 10, 20, and 30 mg/kg i.p. of camphor respectively, and three groups that received these doses without sleep deprivation. For the behavioral analysis, twenty-seven rats were divided into three groups, each receiving the same doses as the previous test.Results and DiscussionOur results showed that there was a decrease in the frequency of brain oscillatory patterns when camphor was administered at 10 mg/kg i.p. whereas there was a dose-dependent increase in the spectral power and distribution following the administration of 20 and 30 mg/kg i.p., with the emergence of Delta, Theta, Alpha, and Beta waves. As for the behavioral analysis, it was demonstrated that testicular relaxation, decreased motility, and light sleep induction also occurred in a dose-dependent manner. Thus, we conclude that camphor administration intensifies occipital electrocorticographic patterns in sleep-deprived rats, and its electrocorticographic and behavioral analysis could indicate a potential as a supporting agent in the insomnia treatment.

Basolateral amygdala oscillations enable fear learning in a biophysical model.

The basolateral amygdala (BLA) is a key site where fear learning takes place through synaptic plasticity. Rodent research shows prominent low theta (~3-6 Hz), high theta (~6-12 Hz), and gamma (>30 Hz) rhythms in the BLA local field potential recordings. However, it is not understood what role these rhythms play in supporting the plasticity. Here, we create a biophysically detailed model of the BLA circuit to show that several classes of interneurons (PV, SOM, and VIP) in the BLA can be critically involved in producing the rhythms; these rhythms promote the formation of a dedicated fear circuit shaped through spike-timing-dependent plasticity. Each class of interneurons is necessary for the plasticity. We find that the low theta rhythm is a biomarker of successful fear conditioning. The model makes use of interneurons commonly found in the cortex and, hence, may apply to a wide variety of associative learning situations.

Decomposing Thermodynamic Dissipation of Linear Langevin Systems via Oscillatory Modes and Its Application to Neural Dynamics

Recent developments in stochastic thermodynamics have elucidated various relations between the entropy production rate (thermodynamic dissipation) and the physical limits of information processing in nonequilibrium dynamical systems. These findings have been actively utilized and have opened new perspectives in the analysis of real biological systems. In neuroscience also, the importance of quantifying entropy production has attracted increasing attention as a means to understand the properties of information processing in the brain. However, the relationship between entropy production rate and oscillations, which is prevalent in many biological systems, is unclear. For example, neural oscillations, such as delta, theta, and alpha waves, play crucial roles in brain information processing. Here, we derive a novel decomposition of the entropy production rate of linear Langevin systems. We show that one of the components of the entropy production rate, called the housekeeping entropy production rate, can be decomposed into independent positive contributions from oscillatory modes. Our decomposition enables us to calculate the contribution of oscillatory modes to the housekeeping entropy production rate. In addition, when the noise matrix of the Langevin equation is diagonal, the contribution of each oscillatory mode is further decomposed into the contribution of each element of the system. To demonstrate the utility of our decomposition, we apply our decomposition to an electrocorticography dataset recorded during awake and anesthetized conditions in monkeys, wherein the properties of oscillations change drastically. We show the consistent trends across different monkeys; i.e., the contribution of oscillatory modes from the delta band are larger in the anesthetized condition than in the awake condition, while those from the higher-frequency bands, such as the theta band, are smaller. These results allow us to interpret the change in neural oscillation in terms of stochastic thermodynamics and the physical limit of information processing. Published by the American Physical Society 2024

Exploring the physiological response differences of β-caryophyllene, linalool and citral inhalation and their anxiolytic potential

Essential oils with β-caryophyllene, citral, and linalool as key compounds often exhibit some anti-anxiety like effects in aromatherapy. However, evidence of the effect of these three compounds through human inhalation remains limited. It is worth exploring their potential anxiolytic effect through the olfactory pathway, and finding out whether the three compounds lead to different physiological responses. A total of 48 subjects were randomly assigned to three odor (β-caryophyllene, citral, and linalool) inhalation groups and one control (odorless jojoba oil) group. Stress stimulation was induced using n-back and mental arithmetic tasks. The odor was administered before the task test session. Assessments including the State-Trait Anxiety Inventory (STAI), electroencephalogram (EEG) activities, facial expressions, several physiological indicators, and a self-report scale of subjective perception of the odor environments were carried out. The changes before and after inhalation, as well as the inter-group differences, were analyzed. Both β-caryophyllene and citral inhalation led to a significant decrease in anxiety levels, while only β-caryophyllene resulted in a notable reduction across both sub-scales of STAI. Following the odor inhalation, heart rate significantly decreased in all three groups, with the β-caryophyllene group exhibiting the most pronounced decline. While the systolic blood pressure of the linalool group demonstrated a statistically significant difference. Regarding facial expressions, β-caryophyllene significantly increased the ratio of 'Happiness' and decreased the ratio of ‘Fear'. In the non-task state, citral reduced the power of frontal alpha, delta, and theta waves while β-caryophyllene had a similar effect. All odor inhalation groups showed increased delta and theta waves after the task compared with the control group, with the β-caryophyllene group having notably lower frontal beta waves. β-Caryophyllene and citral exhibited good anti-anxiety effects. Subjects receiving different odors showed different EEG and physiological responses, indicating the differences in emotional regulation ways among the three compounds.

Ketogenic Diet as a Therapeutic Intervention for Doose Syndrome: A Case Report and Review of Current Evidence

Epilepsy with myoclonic-atonic seizures, also known as Doose syndrome or myoclonic–astatic epilepsy (MAE), represents a challenging pediatric epilepsy syndrome, initially described by Doose et al. in 1970. MAE accounts for 1%–2.2% of juvenile-onset epilepsy cases, characterized by normal early development, followed by seizure onset between 7 months and 6 years. Seizure types include myoclonic, myoclonic-atonic, generalized tonic–clonic, and atypical absences, often accompanied by nonconvulsive status epilepticus. Electroencephalogram typically shows parietal-dominant rhythmic theta activity or a posterior 4 Hz pattern. While conventional antiseizure medications (ASMs) offer some efficacy, outcomes vary widely, and up to 50% of patients may experience refractory seizures and poor intellectual outcomes. The ketogenic diet (KD), a high-fat, low-carbohydrate diet inducing ketosis, has shown promise in reducing seizure frequency and severity in refractory epilepsy, including MAE. This case report describes a successful outcome with a South Indian style KD in a 3-year-old boy with Doose syndrome presenting with 20–25 daily head drop seizures despite multiple ASMs and steroid therapy. Initiation of a home-based KD utilizing traditional household ingredients significantly reduced seizure frequency by 60% within 1 month and 80% within 2 months and was 100% seizure-free in 3rd month following KD, alongside developmental improvements. The KD was well-tolerated, with improved nutritional status. This case underscores KD’s potential as a viable complementary therapy for MAE, emphasizing the need for ongoing parental support and supervision to ensure dietary adherence and optimize outcomes. It is commonly believed that the KD is challenging for the South Indian population to follow due to the high carbohydrate content in South Indian cuisine. However, this case demonstrates that a KD can indeed be tailored around South Indian dishes, highlighting the uniqueness of this approach. When the diet is customized to consider a child’s dietary habits, culture, and cuisine, it becomes easier to follow over the long term, leading to effective seizure control. Further research is necessary to refine dietary protocols and evaluate long-term efficacy in MAE management.

An Empirical Study on the Effect of Blended Scents in Driving Environments From a Neuro-Cognitive Perspective.

An effective method that is easy to implement and widely applicable for improving driving performance and reducing driving risks remains a challenge. Although fragrances are widely used in daily driving, there is a gap between empirical research on everyday blended fragrances and functional fragrances clinical reports. In this study, a deliberately chosen blend of scent without overtly stimulating or functional proven evidence was tested for its potential to enhance performance in a driving environment. Thirty qualified young drivers were recruited to participate in the experiment. They were asked to watch a 15-min first-person perspective driving video to simulate a driving environment and then complete questionnaires and three sets of behavioral experiments while their brain activity was monitored by EEG. Participants in the scented environment exhibited statistically significant advantages in two cognitive tasks during behavioral measures. These findings were effectively supported by the EEG data, showing that beta waves exhibited more activity in the occipital and prefrontal cortex, enhanced theta waves were observed in the prefrontal cortex, and the TAB index characterizing driving fatigue was suppressed in the prefrontal cortex. This empirical evidence highlights the potential of pleasant, natural, and blended scents in enhancing driving performance, suggesting that promoting the aromatherapy while driving as an easily applicable approach in daily life seems justified and expands the application of aromatherapy in daily life.