Thermoreversible Gels Research Articles

Feasibility of injectable thermoreversible gels for use in intramuscular injection of parathyroid autotransplantation

Surgical transplantation of parathyroid tissue into the forearm muscle is one of the most commonly used surgical techniques. While simple, the procedure suffers from drawbacks. This study evaluated the feasibility of thermoreversible gel as an injectable carrier for parathyroid autotransplantation. Polyethyleneglycol-polyalanine-co-phenylalanine (PEG-PAF) thermoreversible gel (sol form at 4°C, gel form at 37°C) were manufactured. Thirty-eight Sprague-Dawley rats were divided into two groups (19 control, C group; 19 experimental, P group). The parathyroid glands of rats were excised. Parathyroid tissues were transplanted into the muscle pocket in sternocleidomastoid muscle in the C group. In the P group, the tissues were injected into the same muscle mixed with 0.3ml thermoreversible gel. The serum levels of parathyroid hormone (PTH), ionized calcium, and phosphorous were measured before surgical procedure, on 7, 21, 56, and 70days after surgery. Histology and immunohistochemistry were performed. Preoperative median PTH level of the C and the P group were 60.80 and 43.85pg/ml, respectively (p=0.641). Seventydays after surgery, median PTH level was 32.8 and 25.61pg/ml, respectively. On day 70, the PTH level was restored by 54% in the C group and 56% in the P group compared to the preoperative value (p=0.620). There were no significant intergroup differences in the ionized calcium/phosphorous level. Histology and immunohistochemistry revealed the successful transplantation of parathyroid tissues into the muscles in both groups. In conclusion, the PEG-PAF-based thermoreversible gel is a good candidate carrier material for intramuscular parathyroid autotransplantation.

Formation of Multicompartment Ion Gels by Stepwise Self-Assembly of a Thermoresponsive ABC Triblock Terpolymer in an Ionic Liquid

Ion gels are materials composed of an ionic liquid immobilized by a network-forming polymer, resulting in a soft solid with high ionic conductivity and tunable mechanical strength. Here, we demonstrate the thermoreversible gelation of a triblock terpolymer poly(ethylene-alt-propylene)-block-poly(ethylene oxide)-block-poly(N-isopropylacrylamide) (PON) in the ionic liquid 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMI][TFSI]). At high temperature, the polymers self-assemble into micelles with P cores and ON coronas. Below the upper critical solution temperature of PNIPAm in this ionic liquid, the N blocks aggregate, resulting in macroscopic gelation. The ion gels were characterized at concentrations of 1, 2, 5, and 10 wt %. Rheological measurements demonstrated that the PON ion gels had a greater percentage of elastically effective strands, leading to significantly higher mechanical strength than for the corresponding NON ion gels. This is consistent with the expectation that an ABC ter...

Structural and physical properties of sanxan polysaccharide from Sphingomonas sanxanigenens

Sphingomonas sanxanigenens, a new species of the genus Sphingomonas, synthesizes extracellular biopolymer termed sanxan. Sanxan polysaccharide was purified from the fermentation broth by Sephacryl S-400 column chromatography. The molecular weight of sanxan polysaccharide was 408kDa by the method of size-exclusion chromatography combined with laser light scattering. Based on FT-IR, periodate oxidation, Smith degradation, composition analysis and nuclear magnetic resonance experiments, the structure of sanxan polysaccharide was elucidated as follows: [Display omitted] The solution of sanxan polysaccharide showed properties of high viscosity and shear-thinning. By cooling hot solutions, sanxan polysaccharide could form elastic thermoreversible gel.

Gelation of wheat arabinoxylans in the presence of Cu+2 and in aqueous mixtures with cereal β-glucans

The effect of copper ions (0.63–0.16mM) on the rheological behavior of arabinoxylan (AX) aqueous solutions was investigated. Moreover, the influence of β-glucan addition (BG, 0.5–3% w/v) on the gelation of mixed AX/BG solutions with and without addition of the peroxidase/H2O2 was examined. Generally, gels formed with inclusion of transition metal-ions differed from those obtained by adding peroxidase/H2O2. Copper ions induced viscosity increase of the AX-solutions and form stronger thermoreversible gels with increasing ion-concentration; optimal gelation was at 15°C. For added β-glucan at levels >1%, the lower the concentration and the higher the molecular weight of β-glucan, the weaker the gelling ability of the mixed AX/BG system treated with peroxidase/H2O2. The polysaccharide-ratio affected both the gelling rate and the network melting temperature, with the β-glucan itself giving the strongest network. Calorimetry provided evidence for existence of β-glucan ordered domains in the mixed gel structures of AX/BG1, indicative of phase separation events.

Poloxamer 407-based intranasal thermoreversible gel of zolmitriptan-loaded nanoethosomes: formulation, optimization, evaluation and permeation studies

Zolmitriptan is the drug of choice for migraine, but low oral bioavailability (<50%) and recurrence of migraine lead to frequent dosing and increase in associated side effects. Increase in the residence time of drug at the site of drug absorption along with direct nose to brain targeting of zolmitriptan can be a solution to the existing problems. Hence, in the present investigation, thermoreversible intranasal gel of zolmitriptan-loaded nanoethosomes was formulated by using mucoadhesive polymers to increase the residence of the drug into the nasal cavity. The preparation of ethosomes was optimized by using 32 factorial design for percent drug entrapment efficiency, vesicle size, zeta potential, and polydispersity index. Optimized formulation E6 showed the vesicle size (171.67 nm) and entrapment efficiency (66%) when compared with the other formulations. Thermoreversible gels prepared by using poloxamer 407 showed the phase transition temperature at 32–33 °C which was in line with the nasal physiological temperature. The optimized ethosomes were loaded into the thermoreversible mucoadhesive gel optimized by varying concentrations of poloxamer 407, carbopol 934, HPMC K100, and evaluated for gel strength, gelation temperature, mucoadhesive strength, in vitro drug release, and ex vivo drug permeation, where G3 and G6 were found to be optimized formulations. In vitro drug release was studied by different kinetic models suggested that G3 (n = 0.582) and G6 (n = 0.648) showed Korsemeyer–Peppas (KKP) model indicating non-Fickian release profiles. A permeation coefficient of 5.92 and 5.9 µg/cm2 for G3 and G6, respectively, revealed very little difference in release rate after 24 h between both the formulations. Non-toxic nature of the gels on columnar epithelial cells was confirmed by histopathological evaluation.

Quasi-Equilibrium Gelation Temperature of Aqueous Methylcellulose

Aqueous methylcellulose (MC) forms hydrogel upon heating and the gel dissolves upon cooling i.e. thermo-reversible gelation. However, the values of the gelation temperature differ depending on the sources of MC samples and on the methods of determination as well. In this study, we have obtained a lower limit of the gelation temperature for a specific MC below which aqueous MC does not form the gel, by dually extrapolating the holding time for gelation to infinite and the mechanical probing force to zero. The gelation temperature obtained in this manner decreased with increasing concentration of the MC; to cite a case, it was located at ca. 21 ̊C for 10 wt%, which is ca. 10 ̊C lower than a conditional value estimated after holding 1 h without these dual extrapolations.

Usefulness of the Behavior of Fibroblast Attachment to Coils in Thermoreversible Gelation Polymer for Aneurysmal Coil Treatment

Background: Post embolization aneurysm reccurence is still a drawback in endovascular coiling for cerebral aneuryms. That stimulated reaearch into new promising embolic materials, as the Thermoreversible Gelation Polymer (TGP) when used as cell culture medium with its temperature related properties. We evaluated proliferative properties of fibroblast cell cultures in association with metalic coils. Methods: Intial assessment of cell viability was done for 3T3 (mouse fibroblast) and A172 (glioma cell) lines for 6 and 16 days in TGP solid phase and media. After that TGP was liquified and cell attachment confirmed. Next, fibroblasts were cultivated under the same conditions adding Guglielmi detachable coils for 24 h and 3 days with TGP, evaluating cell attachment and proliferation on coils. Last, the time course of cell proliferation was evaluated, comparing cell numbers on equal coil surface areas for 1,3,5,7,10,14 and 21 days in TGP. Electron microscopy was used for cell assessment on the coils. Results: Fibroblasts survive and show satisfactory viability in TGP. In liquefied TGP after 6 days and 16days, both cell types temporally extended processes and attached on flask`s bottom. Next after 24 h and 3 days fibroblast cultivation with coils in TGP, cells extended processes and attached to coils, indicating some proliferation and clustering. Last, the day 1 attached cell numbers on coils cultivated in TGP gradually decreased until day 10 and later increased again to just below day 1 level. However, both fibroblast and glioma cells in TGP did not extend processes, move or proliferate, and had spherical shape while in solid TGP. Conclusion: Fibroblasts attachment and long survival on coils in TGP may facilitate aneurysm treatment to achieve better volume embolization rate and improve intra-aneurysmal thrombus organization.

Thermoreversible gel for intrapocket delivery of green tea catechin as a local drug delivery system: An original research.

The periodontal therapies along with systemic antibiotic therapy aim at eliminating the subgingival microbiota to arrest the progression of periodontal diseases. The complete elimination is often difficult, and thus the probability of repopulation after periodontal therapy is also high. The objectives of the study are to develop in situ thermoreversible gelling system of green tea catechins suitable for periodontal pocket administration, which would act as an adjunct to mechanical periodontal therapy. Gel is prepared on a weight basis using a cold process. In vitro drug release pattern is observed through spectrophotometer analysis at 277 nm. The gel is subjected to serial dilution analysis to determine the minimum inhibitory concentration (MIC) and disc diffusion analysis to determine the in vitro antibacterial effectiveness. Release pattern studies showed a complete release of drug from gel occurred by 36 h. A volume of 1.25 mg/ml was determined as MIC required against the periodontal pathogens. Disc diffusion analysis showed a 14 mm zone of inhibition is present around the 75 µl well for all the four species and 12 mm zone of inhibition around the 50 µl well. The advantage of F-127 is its thermoreversible nature that used for in situ gel formulation. Pluronic gel proved to be a promising carrier for prolong and effective release of green tea catechin.

Metallogel formation in aqueous DMSO by perfluoroalkyl decorated terpyridine ligands.

Terpyridine based ligands 1 and 2, decorated with a C8F17 perfluorinated tag, are able to form stable thermoreversible gels in the presence of several d-block metal chloride salts. The gel systems obtained have been characterized by NMR, X-ray diffraction, electron microscopies and Tgel experiments in order to gain insights into the observed different behaviour of the two similar ligands, also in terms of the effect of additional common anionic species.

Controlling thermal gelation properties of novel Tetronic hydrogel-based tissue adhesive

Event Abstract Back to Event Controlling thermal gelation properties of novel Tetronic hydrogel-based tissue adhesive Martin F. Alejos1, Ken Webb1, Olin T. Mefford2 and Jiro Nagatomi1 1 Clemson University, Department of Bioengineering, United States 2 Clemson University, Department of Materials Science and Engineering, United States Introduction: With the recent advancement of laparoscopic and robotic surgeries, increasing demand exists for tissue adhesives and sealants as an alternative to suturing. We and other groups have been investigating synthetic adhesives to overcome limitations shown by those of biological origin (e.g., poor mechanical strength, potential viral transmission and hypersensitive reactions). Tetronic® is a family of 4-arm, PPO-PEO block copolymers, that can form thermoreversible gels in aqueous solutions. We previously reported that Tetronic 1107 was modified to incorporate acrylate (ACR) for chemical crosslinking and N-hydroxysuccinamide (NHS) to enhance tissue bonding; modifications that showed promising adhesive capabilities. However, handling these hydrogels is challenging since they gel rapidly at room temperature and show a relatively high degradation rate[1]. Our goal is to assess the impact of blending bifunctional T1107 with another acrylated Tetronic (T304-ACR) of lower molecular weight and different hydrophilic-lipophilic balance (HLB) on gelation temperature (GT) and degradation rates. Materials and Methods: Hydrogels that consist of 30wt% blends of T1107-ACR-NHS and T304-ACR in PBS were prepared as previously reported[1],[2]. GTs were determined by rheometry (under constant dynamic strain of 1%, f=0.1 Hz, and temperature sweep, 4-40 ˚C) using 40-mm parallel stainless steel plate geometry and 150-µm gap. The values were defined as the crossing point between storage modulus (G’) and loss modulus (G”). Degradation profiles of Tetronic adhesives were defined as the percentage mass loss of crosslinked discs incubated in PBS at pH=7.4 and 37 ˚C[3]. Mass loss is defined as initial weight minus final weight over initial weight. Adhesive properties were evaluated through lap shear test. Specimens (4x1 cm) were prepared by firmly attaching a collagen sheet to a metal strip, and applying the adhesive sample to create a 1 cm2 contact area. Specimens were subjected to uniaxial load at 10 mm/min crosshead speed until failure[1]. Results and Discussion: Thermal gelation of the 100:0 (=T1107-ACR-NHS : T304-ACR) blend occurred at a mean value of 20.0 ˚C. Addition of increasing amounts of T304-ACR led to an increase in GTs; 23.7±2.0˚C, 26.0±1.0˚C and 36.0±2.8˚C for 75:25, 50:50, and 25:75 blends, respectively. The storage modulus G’ determined at 37˚C exhibited a drastic decrease with increasing T304-ACR contents, 49.01±10.34 kPa, 7.94±0.69 kPa, 1.72±0.05 kPa and 0.07±0.09 kPa for 100:0, 75:25, 50:50, and 25:75, respectively. Blending of T304-ACR with T1107-ACT-NHS resulted in higher GTs, which could be expected from the properties of these polymers. T304 and T1107 are of different molecular sizes and HLB, due to different EO to PO ratios, leading to the dissimilar thermogelation behavior. The increase in GT will provide a longer handling time for the adhesive at room temperature prior to application. However, rapid thermal gelation upon contact with warm tissues and organs will be an ideal feature as it will remain in place while chemical crosslinking reaction takes place. Conclusion: Blending different Tetronics has proven to be a possible route to control gelation temperature in Tetronic based adhesives. It must be noted that the temperature control comes at the expense of a drastic change in G’. Modifying polymer concentration may help identifying an ideal combination of GT and mechanical behavior for these Tetronic blends.

Preformulation and characterization of a lidocaine hydrochloride and dexamethasone sodium phosphate thermo-reversible and bioadhesive long-acting gel for intraperitoneal administration

The search for new formulations of anaesthetic agents that allow a localized administration and provide a prolonged effect is of great interest in the multimodal management of postoperative pain. The pre-formulation and characterization of a lidocaine and dexamethasone thermosensitive and bioadhesive long-acting gel for intraperitoneal administration was done as a tool in the management of pain in abdominal surgeries. The pre-formulation process was conducted by a systematic variation of the concentration of the different polymers, until setting it, in a suitable concentration that allowed an adequate gelation temperature. The poloxamer 407 (P407) was used as the main polymer; hydroxypropyl methylcellulose (HPMC) as the bioadhesive agent and polyvinyl pyrrolidone (PVP) to adjust the gelation temperature and physicochemical properties. The formulations were characterized by gelation temperature, pH, viscosity at 25°C and 37°C, gelation time, density and osmolality. Gelation temperature was decreased when increasing the concentration of hydroxypropyl methylcellulose and poloxamer 407, this effect was also observed when adding lidocaine hydrochloride and dexamethasone sodium phosphate to the formulations. The gelation temperature did not have statistically significant relation with the PVP concentration (P-value of 0.6797), even though, there is a tendency in the gelation temperature by varying it. Between the developed formulations, the 12.5/3.3/0.4% (P407/HPMC/PVP) formulation presents an appropriate gelation temperature, a suitable viscosity for administration by syringe, an adequate and stable pH and osmolality to prevent tissue damage and a correct gelation time that allowed the formation of a prolonged release implant.

A Supramolecular Gel Approach to Minimize the Neural Cell Damage during Cryopreservation Process.

The storage method for living cells is one of the major challenges in cell-based applications. Here, a novel supramolecular gel cryopreservation system (BDTC gel system) is introduced, which can observably increase the neural cell viability during cryopreservation process because this system can (1) confine the ice crystal growth in the porous of BDTC gel system, (2) decrease the amount of ice crystallization and cryopreservation system's freezing point, and (3) reduce the change rates of cell volumes and osmotic shock. In addition, thermoreversible BDTC supramolecular gel is easy to be removed after thawing so it does not hinder the adherence, growth, and proliferation of cells. The results of functionality assessments indicate that BDTC gel system can minimize the neural cell damage during cryopreservation process. This method will be potentially applied in cryopreservation of other cell types, tissues, or organs and will benefit cell therapy, tissue engineering, and organs transplantation.

Polymer aerogels for efficient removal of airborne nanoparticles

Abstract This study evaluates the potential of polymer aerogel monoliths in removing airborne nanoparticles. Macroporous monolithic aerogels of δ-form syndiotactic polystyrene (sPS) are synthesized for this purpose via thermoreversible gelation of a solution of sPS in a good solvent followed by supercritical drying. The air permeability and airborne nanoparticle removal efficiency are determined as function of the bulk density of aerogels. The data reveal a power-law dependence of particle removal efficiency and air permeability on bulk density. The data also reveal that efficiency greater than 99.95% can be achieved if the bulk density is kept at 0.042 g/cm3 or higher. These materials show air permeability of the order of 10−10 m2. The gradient density aerogels produced via sequential injections of sPS solutions show improvements in filtration efficiency attributed to additional skin layers. This idea is exploited in designing gradient density aerogel filters that offer higher efficiency and higher air permeability at a much lower bulk density than the single density monoliths of the same thickness.

A rheological investigation of sol–gel transition of hydroxypropyl cellulose with nonionic surfactant sorbitan monopalmitate: Modulation of gel strength by UV irradiation

Abstract The interaction of hydrophobically modified biocompatible polymer hydroxypropyl cellulose (HPC) with nonionic surfactant sorbitan monopalmitate (Span 40) in aqueous solutions have been studied using rheology. The associated physicochemical properties of the HPC+ Span 40 mixed system such as cac, psp and cmc were studied as a function of Span 40 concentration. Steady shear rheological experiments indicate that the HPC as well as HPC+ Span 40 systems possesses two distinct regions in the rheogram Newtonian followed by Pseudoplastic behavior, and behave as plastic systems with the value of m parameter in the empirical Cross model equation η 0 − η α / η − η α = 1 / 1 + C ( γ ) m varying between 0.725–0.675 and 2.24–1.58 in the first and second shear thinning regions. Dynamic rheological results suggest that the microstructural changes that occur during thermo reversible gelation of aqueous solutions of HPC and HPC+ Span 40 systems follow a two step mechanism i.e., gelation followed by precipitation. The thermal stability of the HPC hydrogel decreases as the concentration of Span 40 increases. Moreover, increase in the UV radiation dose on 10% HPC increases its gel strength probably due to the formation of active radicals in the polymer which on further reaction results in formation of highly cross linked gel of high viscoelasticity. However, this phenomena was not observed in 10% HPC solution after addition of Span 40.

Development of rectal delivered thermo-reversible gelling film encapsulating a 5-fluorouracil hydroxypropyl-β-cyclodextrin complex

We have developed a novel 5-Fluorouracil (5FU) formulation for rectal application to improve its therapeutic efficiency in colorectal cancer. The results indicated that 5FU formed an inclusion complex with Hydroxypropyl-β-Cyclodextrin (HP-β-CD). The stoichiometry of the complex was 1:1, with apparent stability constant of 100.4M−1. After investigating physicochemical properties of the 5FU-HP-β-CD complex encapsulated with thermo-reversible gelling film, the optimized formulation P407/P188/HPMC/5FU-HP-β-CD (18.5/2.5/0.2/15%) was selected and evaluated. The result showed that the 5FU-HP-β-CD complex increased the solubility of 5FU, prolonged and enhanced its releasing. As compared to the raw drug, the transport efficiency of the 5FU-HP-β-CD complex itself or entrapped in thermo-reversible gelling film were respectively 7.3- and 6.8-fold increased, and the cellular uptake of 5-FU 4.9- and 5.4-fold elevated. There was no irritation or damage to rectal sites in the 10h treatment period. Therefore, this HP-β-CD based formulation might improve the therapeutic effect of 5FU on colon-rectal cancer.

Mebiolgel, a thermoreversible polymer as a scaffold for three dimensional culture of Huh7 cell line with improved hepatocyte differentiation marker expression and HCV replication

Purpose: A novel three dimensional (3D) culture system purely synthesised from co-polymer which is free from biological contamination for Huh7 cell cultivation and hepatitis C virus (HCV) replication has been attempted. Materials and Methods: Mebiolgel, a thermo-reversible gelation polymer was used as a 3D scaffold for culturing Huh7, a liver carcinoma cell line used in our study. The 3D culture of the cells were infected with cell culture derived HCV. Result: The scaffold supported the cell growth as 3D spheroids for up to 63 days. Moreover mebiolgel was found to be improving the hepatocyte differentiation of Huh7 cells at the transcript level. Three dimensional culture was susceptible for HCV infection, and this was confirmed by detecting the HCV replication intermediate viral core antigen. Conclusion: Mebiolgel based culture system was proven to be suited for 3D culture of Huh7 cells by improvising liver specific genotypic expression and was susceptible for HCV replication. Since mebiolgel based Huh 7 express better hepatocyte differentiation markers genotypically, this can be implemented as an alternate for primary hepatocytes in studies such as viral isolation from patient serum.

Complexation of chitosan and gelatin: From soluble complexes to colloidal gel

ABSTRACTThe formation of soluble and insoluble complexes between chitosan (CH) and gelatin type B (GB) was investigated as a function of pH (3.0–6.5), sodium chloride (NaCl) concentration (0–100 mM), and storage time (up to 40 days). The turbidity of the CH/GB complexes achieved a maximum value at pH 5.5 and increased with time. The increase of ionic strength first intensified the complex formation but then decreased it at higher salt content. After phase separation, the main component of the separated dense phase was water, from 95.5 to 97.8 wt%, depending on NaCl concentration. With increasing storage time, the insoluble phase changed from a liquid-like system to a thermoreversible colloidal gel, as supported by confocal laser scanning microscopy as well as rheological data. The formation of this colloidal gel is explained in terms of electrostatic complexation and hydrogen bonding.

Characteristics and functional properties of gelatin extracted from squid (Loligo vulgaris) skin

The characteristics and functional properties of gelatin extracted from squid skin (Loligo vulgaris), using commercial pepsin at a level of 15 Units/g skin, were investigated. The gelatin extraction yield was 6.82 g/100 g wet basis, showing high protein content and low fat and ash contents. SDS-PAGE of squid skin gelatin showed high band intensity for the major protein components, especially, α- and β-components. The amino acid profile of squid skin gelatin showed a high percentage of imino acids, essential amino acids and hydrophobic amino acids. Fourier transformed infrared spectroscopy (FT-IR) spectra showed helical structure. Squid skin gelatin was able to form a completely thermo-reversible gel. Gelatin possessed interesting functional properties, which were governed by gelatin concentration.

Formulation and evaluation of thermoreversible mucoadhesive in-situ gel for intranasal delivery of naratriptan hydrochloride

Intractable migraine presents a significant treatment challenge due to associated throbbing or pulsating headache, which affects one half of the head and lasts from 2 to 72 h. Naratriptan hydrochloride is an approved drug molecule for migraine headaches, but its use is limited due to its poor bioavailability, reoccurrence of migraine and lesser half-life requiring frequent dosing. Therefore, the objective of present study was to formulate a thermoreversible intranasal gel for drug targeting directly to brain via olfactory lobe pathway thereby improving bioavailability. Gels were formulated by using poloxamer 407 as thermoreversible polymer and carbopol 934 as mucoadhesive polymer. Formulated gels were characterized for gelation temperature, gel strength, mucoadhesive strength, viscosity, in-vitro drug release and ex-vivo permeation study using sheep nasal mucosa. In-vitro and ex-vivo drug release studies suggested that the release rate was directly proportional to the concentration of carbopol 934, whereas poloxamer 407 reduced the rate of drug release. Histopathology study of sheep nasal mucosa showed no signs of damage to columnar epithelial cells, confirming non-toxic nature of the formulated gel. Stability studies performed as per ICH guidelines [Q1A (R2)] suggested that the gels were stable.

Nanostructured Cubosomes in a Thermoresponsive Depot System: An Alternative Approach for the Controlled Delivery of Docetaxel.

The aim of the present study was to develop and evaluate a thermoresponsive depot system comprising of docetaxel-loaded cubosomes. The cubosomes were dispersed within a thermoreversible gelling system for controlled drug delivery. The cubosome dispersion was prepared by dilution method, followed by homogenization using glyceryl monooleate, ethanol and Pluronic® F127 in distilled water. The cubosome dispersion was then incorporated into a gelling system prepared with Pluronic® F127 and Pluronic® F68 in various ratios to formulate a thermoresponsive depot system. The thermoresponsive depot formulations undergo a thermoreversible gelation process i.e., they exists as free flowing liquids at room temperature, and transforms into gels at higher temperatures e.g., body temperature, to form a stable depot in aqueous environment. The mean particle size of the cubosomes in the dispersion prepared with Pluronic® F127, with and without the drug was found to be 170 and 280 nm, respectively. The prepared thermoresponsive depot system was evaluated by assessing various parameters like time for gelation, injectability, gel erosion, and in-vitro drug release. The drug-release studies of the cubosome dispersion before incorporation into the gelling system revealed that a majority (∼97%) of the drug was released within 12 h. This formulation also showed a short lag time (∼3 min). However, when incorporated into a thermoresponsive depot system, the formulation exhibited an initial burst release of ∼21%, and released only ∼39% drug over a period of 12 h, thus indicating its potential as a controlled drug delivery system.