Thermophilic Treatment Research Articles

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This article announces that the International Water Association (IWA) awarded the national environmental engineering firm Brown and Caldwell (BC) the 2008 Global Project Innovation Superior Achievement Award. The award, presented at the IWA World Water Congress and Exhibition in Vienna, Austria, was presented for the Columbus Biosolids Flow‐Through Thermophilic Treatment (CBFT3) and Cogeneration System Project built for the Columbus Water Works (CWW) in Columbus, Georgia.

Azo Dye Reduction by Mesophilic and Thermophilic Anaerobic Consortia

The reduction of the azo dye model compounds Reactive Red 2 (RR2) and Reactive Orange 14 (RO14) by mesophilic (30 degrees C) and thermophilic (55 degrees C) anaerobic consortia was studied in batch assays. The contribution of fermentative and methanogenic microorganisms in both temperatures was evaluated in the presence of the fermentative substrate glucose and the methanogenic substrates acetate, H2/CO2, methanol, and formate. Additionally, the effect of the redox mediator riboflavin on electron shuttling was assessed. We concluded that the application of thermophilic anaerobic treatment is an interesting option for the reductive decolorization of azo dyes compared to mesophilic conditions. The use of high temperature may decrease or even take the place of the need for continuous redox mediator dosage in bioreactors, contrarily to the evident effect of those compounds on dye reduction under mesophilic conditions. Both fermenters and methanogens may play an important role during reductive decolorization of dyes, in which mediators are important not only for allowing the different microbes to participate more effectively in this complex reductive biochemistry but also for assisting in the competition for electrons between dyes and other organic and inorganic electron acceptors.

Effect of temperature on microbial community of a glucose-degrading methanogenic consortium under hyperthermophilic chemostat cultivation

We continuously fed an anaerobic chemostat with synthetic wastewater containing glucose as the sole source of carbon and energy to study the effects of temperature on the microbial community under hyperthermophilic (65-80 degrees C) conditions. Methane was produced normally up to 77.5 degrees C at a dilution rate of 0.025 d(-1). However, the concentration of microorganisms and the rate of gas production decreased with increasing operation temperature. The microbial community in the chemostat at various temperatures was analyzed based on the 16S rRNA gene using molecular biological techniques including clone library analysis and denaturing gradient gel electrophoresis (DGGE). Aceticlastic methanogens related to Methanosarcina thermophila were detected at 65 degrees C and hydrogenotrophilic methanogens related to Methanothermobacter thermautotrophicus were the dominant methanogens between 70 degrees C to 77.5 degrees C. Bacteria related to Clostridium stercorarium and Thermoanaerobacter subterraneus comprised the dominant glucose-fermenting bacteria at temperatures of 65 degrees C and above, respectively. Bacteria related to Thermacetogenium phaeum and to Tepidiphilus margaritifer and Petrobacter succinatimandens were the dominant acetate-oxidizing bacteria at 70 degrees C and at 75-77.5 degrees C, respectively. The results suggested that, at temperatures of 70 degrees C and above, methane production via the aceticlastic pathway was negligible and indirect methanogenesis from acetate was dominant. Since acetate oxidation is a rate limiting step and a higher temperature favors the hydrolysis and acid formation, a two stage fermentation process, acidogenic and methanogenic fermentation stages operated under different temperatures, should be more suitable for the thermophilic anaerobic treatment at temperatures above 65 degrees C.

ChemInform Abstract: Offgas Recycle for Increased Heat Production from Aerobic Thermophilic Treatment of Swine Waste: Pilot Studies and Full‐Scale Design

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

EFFECT OF TEMPERATURE ON SOLIDS REDUCTIONS AND ON DEGRADATION KINETICS DURING THERMOPHILIC AEROBIC DIGESTION OF A SIMULATED SLUDGE

Laboratory‐scale experiments were conducted to determine the influence of higher thermophilic temperatures on thermophilic aerobic digestion treatment of a simulated sludge. The efficiency of the process was evaluated in respect of solids removal and degradation rate constants at four thermophilic temperatures. Batch runs were operated at a retention time of one day and temperatures of 65, 70, 72 and 75 °C. The results indicated that temperature increase did not impart any significant benefits to the digestion operation in terms of suspended solids and biochemichal oxygen demand reduction. The findings from this research also suggested that the treatment would not appear to benefit from temperatures higher than 65 °C, as classically suggested by Van’t Hoff‐Arrhenius. Therefore, increase of thermophilic temperature in the tested 65‐–75 °C range does not enhance the efficiency of thermophilic, aerobic sludge digestion treatment.

Reactive Blue 4 Decolorization under Mesophilic and Thermophilic Anaerobic Treatments

Anaerobic decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) was studied to evaluate the factors involved in dye-reducing behaviors such as dye concentration, co-substrate, treatment temperature, salt content, and dye-reducing microbial consortia. The experiment was conducted using digested sludge treated under mesophilic (35 degrees C) and thermophilic (55 degrees C) conditions. The results indicated that the thermophilic treatment gave higher potential for this dye decolorization compared with the mesophilic one. A reduced form of RB4 did not show an auto-oxidizing reaction but treated RB4 dye was shown in light yellow color, the intensity of which was related to the initial concentration of the dye used in the treatments. Starch, which showed similar decolorizing efficiency under thermophilic conditions, could be used as a co-substrate instead of glucose for the purpose of operating cost reduction. Due to the high content of salt contained in dye wastewater, the effect of salt (NaCl) was investigated. Results showed that decolorization could be accelerated with a concentration of NaCl lower than 200 mM, but the decolorization was inhibited by high concentrations of salt. The presence of RB4 inhibited methane productivity, while total organic carbon (TOC) reduction was similar to control, without dye addition. Increasing the temperature accelerated the decolorizing potential and TOC reduction. The evaluation of dye-reducing microbial consortia was done with acidogen and methanogen inhibitors which acidogenesis microorganism was dominant in RB4 decolorization.

Survival of selected pathogens in diluted sludge of a thermophilic waste treatment plant and in NaCl-solution under aerobic and anaerobic conditions

Decimal reduction times (DRT or D-value) of Campylobacter jejuni, Salmonella enterica (formerly Salmonella choleraesuis) serovar Senftenberg, Escherichia coli, and Listeria monocytogenes were determined in two different matrices, diluted fermenter sludge (DFS) and 0.95% NaCl-solution (NaCl) at 50 degrees C, both under aerobic and anaerobic conditions. Depending on aeration, matrix composition, and the respective organism, the D-values varied between 10min and more than 15h. Generally the viability of bacteria decreased faster in DFS compared to NaCl-solution and under aerobic compared to anaerobic conditions. After 24h no viable cells could be detected in DFS, both under aerobic as well as under anaerobic conditions, whereas viable cells were still found in NaCl solutions. In both matrices the detection limits determined by means of PCR-based and classical microbiological methods were compared and pointed to lower detection limits of the latter methods. Results of the present investigation show that test organisms were far from surviving several days in DFS whereas hydraulic retention times normally used for thermophilic anaerobic digestion are in the range of 2 weeks. However, an underestimation of survival rates of the test organisms seems probable when applying aerobic standard methods.

Thermophilic anaerobic digestion of source-sorted organic fraction of municipal solid waste

The influence of different organic fraction of municipal solid wastes during anaerobic thermophilic (55°C) treatment of organic matter was studied in this work: food waste (FW), organic fraction of municipal solid waste (OFMSW) and shredded OFMSW (SH_OFMSW). All digester operated at dry conditions (20% total solids content) and were inoculated with 30% (in volume) of mesophilic digested sludge. Experimental results showed important different behaviours patterns in these wastes related with the organic matter biodegradation and biogas and methane production. The FW reactor showed the smallest waste biodegradation (32.4% VS removal) with high methane production (0.18 LCH4/gVS); in contrast the SH_OFMSW showed higher waste biodegradation (73.7% VS removal) with small methane production (0.05 LCH4/g VS). Finally, OFMSW showed the highest VS removal (79.5%) and the methane yield reached 0.08 LCH4/g VS. Therefore, the nature of organic substrate has an important influence on the biodegradation process and methane yield. Pre-treatment of waste is not necessary for OFMSW.

Comparative decolorizing efficiency of textile dye by mesophilic and thermophilic anaerobic treatments

Anaerobic decolorization of two kinds of dye, viz., azo type represented by Methyl Orange (MO) and anthraquinone type represented by Reactive Blue 4 (RB4) was performed using digested sludge under mesophilic (35°C) and thermophilic (55°C) conditions. Decolorization of dye was investigated to compare the efficiency and extent of decolorization, and additionally to evaluate the effect of temperature and dye on decolorizing microorganisms. Glucose was used as an electron donor in terms of co-substrate without the addition of other nutrients. Under thermophilic treatment, high efficiency of decolourisation was shown in both of the dyes at high concentration, 1000 mg·L-1of MO and 600mg·L-1 of RB4 compared with mesophilic treatment. MO, 200mg·L-1, was decolorized 95-98% under both mesophilic and thermophilic conditions. High decolorizing efficiency of RB4, 100mg·L-1, obtained under thermophilic conditions, was 80% when compared with mesophilic conditions (70%). The reduced form of MO showed an auto-oxidizing effect with pink to violet color when exposed to air, while RB4 showed no auto-oxidizing reaction. The inhibition of MO was effected on sugar conversion to fatty acids, and CH4 productivity resulted in slow reduction of TOC. While RB4 inhibited only on methane productivity, in which TOC reduction was similarly used as control. Due to the increase of temperature, methanogenesis was inhibited and which low CH4 production, whereas an increase of decolorizing efficiency on both dye decolorization and a high rate of TOC reduction was observed.

Occurrence and levels of indicators and selected pathogens in different sludges and biosolids

Determine the occurrence and levels of pathogens and indicators in raw and treated sludges and compare their persistence after two different treatments. Helminth ova, Cryptosporidium spp., Salmonella spp., enteroviruses, and bacterial and viral indicators were determined in raw sludges and biosolids produced after mesophilic and thermophilic treatments. Except Salmonella, all of the parameters were quantified. Helminth ova were found at very low concentrations even in raw sludges. Viable Cryptosporidium oocysts were still present in most samples of treated sludges. Faecal coliforms, spores of sulphite-reducing clostridia (SSRC), and somatic coliphages were the only indicators with values above their detection limits in most of the samples. Pathogens were still detected in some treated sludge samples. SSRC were the most resistant micro-organisms to treatments and hence may be an indicator for the reduction of protozoan oocysts. Somatic coliphages constitute an alternative as viral indicators due to their detection in sludges before and after treatment. Because of the persistence of some pathogens after sludge treatments, additional indicators are needed. SSRC and somatic coliphages are good candidates. Easy and inexpensive methods for the determination of these indicators are feasible both in industrialized and developing countries.

Thermophilic treatment of acidified and partially acidified wastewater using an anaerobic submerged MBR: Factors affecting long-term operational flux

The long-term operation of two thermophilic anaerobic submerged membrane bioreactors (AnSMBRs) was studied using acidified and partially acidified synthetic wastewaters. In both reactors, cake formation was identified as the key factor governing critical flux. Even though cake formation was observed to be mostly reversible, particle deposition proceeds fast once the critical flux is exceeded. Very little irreversible fouling was observed during long-term operation, irrespective of the substrate. Critical flux values at the end of the reactors operation were 7 and 3 L/m 2 h for the AnSMBRs fed with acidified and partially acidified wastewaters, respectively, at a gas superficial velocity of 70 m/h. Small particle size was identified as the responsible parameter for the low observed critical flux values. The degree of wastewater acidification significantly affected the physical properties of the sludge, determining the attainable flux. Based on the fluxes observed in this research, the membrane costs would be in the range of 0.5 €/m 3 of treated wastewater. Gas sparging was ineffective in increasing the critical flux values. However, preliminary tests showed that cross-flow operation may be a feasible alternative to reduce particle deposition.

Isolation, identification and characterization of effective bacteria on bioremediation from the waste parts of Stevia rebaudiana Berutoni

Four thermophilic strains were isolated by thermophilic treatment (2 days at 55 °C) of the stevia-powder, and were identified by the sequence analysis of the 16S rRNA gene; Ureibacillus thermosphaericus (FERM P-20039), Bacillus thermoamylovorans-1, B. thermoamylovorans-2 and Thermoactinomyces candidus. Since all of them have nitrate-reducing and ammonium-forming ability, it is highly possible that they can first produce nitrous acid from nitrate followed by the generation of ammonium. Only U. thermosphaericus had significantly large growing ability in the medium contained 1000 ppm of “Lannate ®-45 water lenitive” (carbamate insecticide) and 400 ppm of “Ortran ®” (organophosphorus insecticide) compared with the same concentration-level in the contrast medium without adding the pesticide.

Quantification of Cryptosporidium parvum in anaerobic digesters treating manure by (reverse-transcription) quantitative real-time PCR, infectivity and excystation tests

The survival of Cryptosporidium parvum oocysts in anaerobic digesters treating manure was investigated for mesophilic, thermophilic, and a combined treatment (mesophilic-thermophilic-mesophilic) under different retention times of oocysts in the reactors. C. parvum DNA was extracted with an optimised protocol, and its amount determined by quantitative real-time PCR (qPCR). Results indicated noteworthy differences in DNA content after the different treatments. DNA was not degraded during the process. However, excystation and infectivity tests showed a reduction of viable oocyst numbers of > or = 2 and > or = 5 log units after the thermophilic treatment in two different experiments. Thus qPCR-targeting DNA can overestimate the number of oocysts that survive and remain viable after anaerobic digestion. However, targeting DNA is suitable to indicate the presence or absence of oocysts. Reverse transcription qPCR (RT-qPCR) targeting C. parvum hsp70 mRNA successfully indicated the presence of viable fraction of oocysts.

Fertilizer potential of liquid and solid effluent from thermophilic anaerobic digestion of poultry waste

Thermophilic anaerobic treatment of poultry litter produces an effluent stream of digested materials that can be separated into solid and liquid fractions for use as a crop fertilizer. The majority of the phosphorus is partitioned into the solid fraction while the majority of the nitrogen is present in the liquid fraction in the form of ammonium. These materials were tested over six years as an alternative fertilizer for the production of vegetable, fruit, and grassland crops. Application of the solids as a field crop fertilizer for vegetables and blueberries resulted in lower yields than the other fertilizer treatments, but an increase in soil phosphorus over a four-year period. Application of the digested liquids on grass and vegetable plots resulted in similar or superior yields to plots treated with commercially available nitrogen fertilizers. Hydroponic production of lettuce using liquid effluent was comparable to a commercial hydroponic fertilizer regime; however, the effluent treatment for hydroponic tomato production required supplementation and conversion of ammonium to nitrate. While not a total fertilizer solution, our research shows the effectiveness of digested effluent as part of a nutrient management program which could turn a livestock residuals problem into a crop nutrient resource.

Thermophilic aerobic wastewater treatment of waste metalworking fluids

AbstractAn investigation into aerobic biological wastewater treatment was conducted over the temperature range 25–75°C in a semi‐batch bioreactor using waste metalworking fluids (MWFs) generated from Liverpool John Moores University's engineering workshop. The best treatment performance in terms of chemical oxygen demand (COD) removal occurred at 50°C (97.27%), with an operational time of 77.5 h. Cell viability was observed throughout the operation and it was found that higher temperature did not directly correlate to low viability. Effluent turbidity reduced considerably from 35 to 50°C and increased thereafter. Even when the system encountered alkaline shock, the overall performance was not affected, thus indicating how stable the system was. The results of this study indicate that an existing industrial‐activated sludge plant could be used to treat waste MWFs under thermophilic conditions. The COD reduction was significant but further investigation into biodegradation of recalcitrant compounds is needed to achieve optimum performance.

On-site treatment of high-strength alcohol distillery wastewater by a pilot-scale thermophilic multi-staged UASB (MS-UASB) reactor

A pilot-scale multi-staged UASB (MS-UASB) reactor with a working volume of 2.5 m3 was operated for thermophilic (55 degrees C) treatment of an alcohol distillery wastewater for a period of over 600 days. The reactor steadily achieved a super-high rate COD removal, that is, 60 kgCOD m(-3) d(-1) with over 80% COD removal. However, when higher organic loading rates were further imposed upon the reactor, that is, above 90 kgCOD m(-3) d(-1) for barely-based alcohol distillery wastewater (ADWW) and above 100 kgCOD m(-3) d(-1) for sweet potato-based ADWW, the reactor performance somewhat deteriorated to 60 and 70% COD removal, respectively. Methanogenic activity (MA) of the retained sludge in the thermophilic MS-UASB reactor was assessed along the time course of continuous run by serum-vial test using different substrates as a vial sole substrate. With the elapsed time of continuous run, hydrogen-utilizing MA, acetate-utilizing MA and propionate-fed MA increased at maximum of 13.2, 1.95 and 0.263 kgCOD kgVSS(-1) d(-1), respectively, indicating that propionate-fed MA attained only 1/50 of hydrogen-utilizing MA and 1/7 of acetate-utilizing MA. Since the ADWW applied herewith is a typical seasonal campaign wastewater, the influence of shut-down upon the decline in sludge MA was also investigated. Hydrogen-utilizing MA and acetate-utilizing MA decreased slightly by 3/4, during a month of non-feeding period, whereas propionate-fed MA were decreased significantly by 1/5. Relatively low values of propionate-fed MA and its vulnerability to adverse conditions suggests that the propionate degradation step is the most critical bottleneck of overall anaerobic degradation of organic matters under thermophilic condition.

Conventional and thermophilic aerobic treatability of high strength oily pet food wastewater using membrane-coupled bioreactors

Although thermophilic treatment systems have recently gained considerable interest, limited information exists on the comparative performances of membrane-coupled bioreactors (MBR) at thermophilic and conventional conditions. In this study aerobic MBRs operating at room temperature (20 °C) and at lower thermophilic range (45 °C) were investigated for the treatment of dissolved air flotation (DAF) pretreated pet food wastewater. The particular wastewater is characterized by oil and grease (O & G) concentrations as high as 6 g/L, COD of 51 g/L, BOD of 16 g/L and volatile fatty acid (VFA) of 8.3 g/L. The performances of the two systems in terms of COD, BOD and O & G removal at varying hydraulic retention time (HRT) are compared. COD removal efficiencies in the thermophilic MBR varied from 75% to 98% and remained constant at 94% in the conventional MBR. The O & G removal efficiencies were 66–86% and 98% in the thermophilic and conventional MBR, respectively. Interestingly, high concentrations of VFA were recorded, equivalent to 50–73% of total COD, in the thermophilic MBR effluent. The observed yield in the thermophilic MBR was 40% of that observed in the conventional MBR.

Aplicação conjunta de tratamento anaeróbio termofílico por lodo granular e de mediadores redox na remoção de cor de águas residuárias têxteis

Investigou-se o efeito de diferentes mediadores redox na remoção de cor de corantes azo pelo uso de lodo granular anaeróbio sob condições mesofílicas (30ºC) e termofílicas (55ºC). Adicionalmente, estudou-se em ambas temperaturas, o efeito de diferentes doadores de elétrons nos processos de descoloração. Comprovou-se em tais processos um impacto marcante da adição de concentrações catalíticas de mediadores redox, aumentando a cinética da reação em até 1 ordem de magnitude. Comparado com tratamento mesofílico, remoções de cor sob condições termofílicas foram extremamente aceleradas, além de o impacto dos mediadores redox ser consideravelmente diminuído à 55ºC. Por exemplo, em experimento de fluxo contínuo, eficiências de remoção em torno de 95% e 56% foram obtidas à 55ºC e 30ºC, respectivamente, na ausência de qualquer mediador redox. Hidrogênio se mostrou extremamente efetivo como doador de elétrons para o processo de descoloração redutiva de corantes azo quando comparado com glicose, formiato e acetato. Os resultados obtidos nesta investigação trazem boas perspectivas para o uso conjunto de reatores anaeróbios sob condições termofílicas e de mediadores redox no pré-tratamento das águas residuárias de indústrias têxteis.

Optimization of the aerobic biological treatment of thermophilically treated refractory wastewater

A pilot scale conventional activated sludge was operated for over 600 days to study its effectiveness at further remediating the effluent of an existing industrial site's thermophilic biological treatment stage. During the course of the study, the activated sludge was able to further biodegrade the contaminants in the incoming industrial wastewater in terms of both BOD and nitrogen reductions at varying hydraulic and solids retention times, despite elevated concentrations of soluble copper being present. A limiting hydraulic retention time (HRT) for BOD removal of 1.5 days was observed as well as the loss of nitrification occurred at a solids retention time (SRT) of approximately 6 days. Biokinetic coefficients were determined with the maximum rate of substrate utilization per unit mass of microorganisms, k, of 0.14 mg VSS/(mg sBOD-d) and the Monod half velocity constant, K s, of 9.4 mg sBOD/L. Simultaneous nitrification and denitrification (SND) of the nitrogenous compounds found in this wastewater was observed throughout the majority of the experimentation while the bulk DO in the system was greater than 1 mg/L. The activated sludge was estimated to contain soluble copper on the order of 1 mg/L throughout the course of operation with no apparent detriment to nitrification. Additionally, the activated sludge was able to biologically remove the main solvents found in the influent wastewater. The removals of trace levels of N-nitrosodimethylamine (NDMA) were also observed.

Thermophilic treatment by anaerobic granular sludge as an effective approach to accelerate the electron transfer and improve the reductive decolorization of azo dyes in bioreactors

The effects of temperature, hydraulic retention time (HRT), and the redox mediator, anthraquinone-2,6-disulfonate (AQDS), on electron transfer and subsequent reductive decolorization of dyes from textile wastewater was assessed in mesophilic and thermophilic anaerobic bioreactors. The results clearly show that compared to mesophilic anaerobic treatment, thermophilic treatment at 55 degrees C is an effective approach for increasing the electron transfer capacity in bioreactors, and thus improving the decolorization rates. At an HRT of 2.5 h and in the absence of AQDS, the color removal was 5.3-fold higher at 55 degrees C compared to 30 degrees C. Furthermore, similar decolorizations were found at 55 degrees C between the AQDS-free and AQDS-supplemented reactors, whereas a significant difference (up to 3.6-fold) on dye reduction occurred at 30 degrees C.