Rheological tests performed under conditions relevant to those experienced during proof and oven rise are necessary for understanding the mechanisms of dextran addition on wheat dough baking performance. This study evaluated the effect of a high molecular weight ( Mw ) dextran, produced in situ by Weissella confusa A16 or externally added, on wheat dough rheological properties including, (i) proofing behavior using a maturograph; (ii) bi-axial extensional profile using a dough inflation system; and (iii) viscoelastic characters (proof) and thermo-mechanical properties (simulated baking) by dynamic mechanical thermal analysis (DMA). The externally-added dextran increased dough elasticity, tenacity, and viscoelastic characters, but reduced dough extensibility at bubble rupture. DMA tests of doughs under dynamic heating conditions showed a sharp increase of elastic and loss moduli until maximum between 75 and 95 °C, accompanied by a drastic decrease of Tan δ (dough stiffening). Dextran addition exhibited a weakening effect on the dough thermal properties i.e., decreased peak moduli during heating. On the other hand, the mild acidic conditions during sourdough fermentation favored the activity of in situ produced dextran, conferring significantly improved thermal-mechanical properties and dough extensibility. This may explain the superior ability of in situ produced dextran to improve bread volume and crumb softness compared to the external-added dextran. By analyzing rheological parameters, we showed that the maximum proofing moduli in DMA, fermentation stability, dough level, and elasticity in maturogram were predictors of good baking quality. Overall, our study provides the mechanistic underpinning and optimum of dextran as a natural improver of bread quality. • Rheological properties of doughs are strongly associated with baking performance. • Dextran increases dough elasticity and tenacity but reduces dough extensibility. • Dextran added externally exhibits a thermal weakening effect on wheat dough. • Dextran produced in situ is preferred in improving dough rheology and bread quality. • Favorable effects of in situ -produced dextran are mediated by mild acidification.