Thermal Stimulation Research Articles

Thermal Effect on Heat Shock Protein 70 Family to Prevent Atherosclerotic Cardiovascular Disease.

Heat shock protein 70 (HSP70) is a chaperone protein induced by various stresses on cells and is involved in various disease mechanisms. In recent years, the expression of HSP70 in skeletal muscle has attracted attention for its use as a prevention of atherosclerotic cardiovascular disease (ASCVD) and as a disease marker. We have previously reported the effect of thermal stimulation targeted to skeletal muscles and skeletal muscle-derived cells. In this article, we reported review articles including our research results. HSP70 contributes to the improvement of insulin resistance as well as chronic inflammation which are underlying pathologies of type 2 diabetes, obesity, and atherosclerosis. Thus, induction of HSP70 expression by external stimulation such as heat and exercise may be useful for ASCVD prevention. It may be possible to induce HSP70 by thermal stimulus in those who have difficulty in exercise because of obesity or locomotive syndrome. It requires further investigation to determine whether monitoring serum HSP70 concentration is useful for ASCVD prevention.

Mild Photothermal-Stimulation Based on Injectable and Photocurable Hydrogels Orchestrates Immunomodulation and Osteogenesis for High-Performance Bone Regeneration.

A photoactivated bone scaffold integrated with minimally invasive implantation and mild thermal-stimulation capability shows great promise in the repair and regeneration of irregularly damaged bone tissues. Developing multifunctional photothermal biomaterials that can simultaneously serve as both controllable thermal stimulators and biodegradable engineering scaffolds for integrated immunomodulation, infection therapy, and impaired bone repair remains an enormous challenge. Herein, an injectable and photocurable hydrogel therapeutic platform (AMAD/MP) based on alginate methacrylate, alginate-graft-dopamine, and polydopamine (PDA)-functionalized Ti3C2 MXene (MXene@PDA) nanosheets is rationally designed for near-infrared (NIR)-mediated bone regeneration synergistic immunomodulation, osteogenesis, and bacterial elimination. The optimized AMAD/MP hydrogel exhibits favorable biocompatibility, osteogenic activity, and immunomodulatory functions in vitro. The proper immune microenvironment provided by AMAD/MP could further modulate the balance of M1/M2 phenotypes of macrophages, thereby suppressing reactive oxygen species-induced inflammatory status. Significantly, this multifunctional hydrogel platform with mild thermal stimulation efficiently attenuates local immune reactions and further promotes new bone formation without the addition of exogenous cells, cytokines, or growth factors. This work highlights the potential application of an advanced multifunctional hydrogel providing photoactivated on-demand thermal cues for bone tissue engineering and regenerative medicine.

Debonds and water-filled defects detection in honeycomb sandwich composites based on pulse infrared thermography ndt technique

Honeycomb Sandwich Composites (HSCs) have been extensively used in aerospace, automotive and shipbuilding industries due to their light weight, high temperature resistance, high strength and fatigue resistance. In this study, the infrared thermography was used to detect debonds and water-filled defects in HSCs specimens under pulsed thermal stimulation. To improve the efficiency of defects detection, dynamic thermal tomography (DTT), principal component analysis (PCA) and total harmonic distortion (THD) techniques were applied to the raw infrared image sequences. The results show that, in the inspection of HSCs, the defect identification results can be improved by using the image processing techniques mentioned above, while the signal-to-noise ratio (SNR) can be significantly improved by means of the THD technique. It is confirmed that debonds and water-filled defects in the HSCs can reliably be detected and identified by using the technique of pulse infrared thermography nondestructive testing.

Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus.

Neuropathic pain after spinal cord injury (SCI) remains a common and thorny problem, influencing the life quality severely. This study aimed to elucidate the reorganization of the primary sensory cortex (S1) and the regulatory mechanism of the lateral parabrachial nucleus (lPBN) in the presence of allodynia or hyperalgesia after left spinal cord hemisection injury (LHS). Through behavioral tests, we first identified mechanical allodynia and thermal hyperalgesia following LHS. We then applied two-photon microscopy to observe calcium activity in S1 during mechanical or thermal stimulation and long-term spontaneous calcium activity after LHS. By slice patch clamp recording, the electrophysiological characteristics of neurons in lPBN were explored. Finally, exploiting chemogenetic activation or inhibition of the neurons in lPBN, allodynia or hyperalgesia was regulated. The calcium activity in left S1 was increased during mechanical stimulation of right hind limb and thermal stimulation of tail, whereas in right S1 it was increased only with thermal stimulation of tail. The spontaneous calcium activity in right S1 changed more dramatically than that in left S1 after LHS. The lPBN was also activated after LHS, and exploiting chemogenetic activation or inhibition of the neurons in lPBN could induce or alleviate allodynia and hyperalgesia in central neuropathic pain. The neuronal activity changes in S1 are closely related to limb pain, which has accurate anatomical correspondence. After LHS, the spontaneously increased functional connectivity of calcium transient in left S1 is likely causing the mechanical allodynia in right hind limb and increased neuronal activity in bilateral S1 may induce thermal hyperalgesia in tail. This state of allodynia and hyperalgesia can be regulated by lPBN.

The effects of adrenal gland hormones depletion on thermal pain in animal model

The studies have shown that adrenal hormones may exert modulatory effects on pain perception in animals. To investigate the analgesic effects of adrenal gland hormones in response to thermal nociceptive stimulation in rats, the present study evaluated the effects of adrenalectomy on the thermal pain threshold. The rats were divided into control and adrenalectomized female and male groups. Adrenalectomy was achieved using a dorsal midline incision at the level of the first through third lumbar vertebrae and removal of adrenal gland. The tail immersion assay was used for evaluating the analgesic potential of adernalectomy. To measure thermal pain threshold, the end of the tail was placed in a 50°C water bath and the pain threshold was measured as the time required to elicit a flick of the tail. Thermal pain threshold was measured 10 and 30 days after adrenalectomy and compared between adrenalectomized and control intact rats. Thermal pain threshold did not significantly change in male and female rats 10 and 30 days after adrenalectomy compared to control group. These data indicate that depletion of adrenal hormones 10 or 30 days after adrenalectomy does not significantly modulate the pain-induced behavioral responses related to thermal nociception.

A Comparison of Functional Connectivity in the Human Brainstem and Spinal Cord Associated with Noxious and Innocuous Thermal Stimulation Identified by Means of Functional MRI.

The somatosensory system is multidimensional and processes important information for survival, including the experience of pain. The brainstem and spinal cord serve pivotal roles in both transmitting and modulating pain signals from the periphery; although, they are studied less frequently with neuroimaging when compared to the brain. In addition, imaging studies of pain often lack a sensory control condition, failing to differentiate the neural processes associated with pain versus innocuous sensations. The purpose of this study was to investigate neural connectivity between key regions involved in descending modulation of pain in response to a hot, noxious stimulus as compared to a warm, innocuous stimulus. This was achieved with functional magnetic resonance imaging (fMRI) of the brainstem and spinal cord in 20 healthy men and women. Functional connectivity was observed to vary between specific regions across painful and innocuous conditions. However, the same variations were not observed in the period of anticipation prior to the onset of stimulation. Specific connections varied with individual pain scores only during the noxious stimulation condition, indicating a significant role of individual differences in the experience of pain which are distinct from that of innocuous sensation. The results also illustrate significant differences in descending modulation before and during stimulation in both conditions. These findings contribute to a deeper understanding of the mechanisms underlying pain processing at the level of the brainstem and spinal cord, and how pain is modulated.

A Gain-of-function Mutation in the Gating Domain of ITPR1 Impairs Motor Movement and Increases Thermal and Mechanical Sensitivity

Inositol 1,4,5-trisphosphate receptor type 1 (ITPR1) is an intracellular Ca2+ release channel important for a number of fundamental cellular functions. Consistent with its critical physiological significance, mutations in ITPR1 are associated with disease. Surprisingly, nearly all the disease-associated ITPR1 mutations characterized to date are loss of function. Despite the paucity of ITPR1 gain-of-function (GOF) mutations, enhanced ITPR1 function as a result of dysregulation by ITPR1 interacting proteins is thought to be associated with ataxia, learning and memory impairments, Alzheimer’s disease (AD) progression, and chronic pain. However, direct evidence for the role of ITPR1 GOF in disease is lacking. To determine whether GOF in ITPR1 itself has pathological ramifications, we employed a newly developed mouse model expressing an ITPR1 mutation in the gating domain of the channel, D2594K, that markedly increased the channel’s sensitivity to activation by IP3. Behavioral studies showed that the ITPR1-D2594K+/- mutant mice displayed motor deficits and reduced muscle strength. However, the ITPR1-D2594K+/- mutation did not significantly alter hippocampal learning and memory and did not change learning and memory impairments when crossed with the 5xFAD AD model mice. On the other hand, ITPR1-D2594K+/- mice exhibited increased sensitivity to thermal and mechanical stimulation compared to WT. Interestingly, R-carvedilol treatment attenuated the enhanced thermal and mechanical nociception in ITPR1-D2594K+/- mice. Thus, the ITPR1-D2594K+/- mutation in the channel’s gating domain has a marked impact on motor movements and pain perception, but little effect on hippocampal learning and memory.

Nano t-Se Peninsulas Embedded in Natively Oxidized 2D TiSe2 Enable Uniform and Fast Memristive Switching.

Memristive devices, regardless of their potential applications in memory and computing scenarios, still suffer from large cycle-to-cycle and device-to-device variations due to the stochastic growth of conductive filaments (CFs). In this work, we fabricated a crossbar memristor using the 2D TiSe2 material and then oxidized it into TiO2 in the atmosphere at a moderate temperature. Such a mild oxidation approach fails to evaporate all Se into the air, and after further annealing using thermal or electrical stimulations, the remnant Se atoms gather near the interfaces and grow into nanosized crystals with relatively high conductivity. The resulting peninsula-shaped nanocrystals distort the electric field, forcing CFs to grow on them, which could largely confine the location and length of CFs. As a result, this two-terminal TiSe2/TiO2/TiSe2 device exhibits excellent resistive switching performance with a fairly low threshold voltage (Vset < 0.8 V, Vreset > 0.55 V) and high cycle-to-cycle consistency, enabling resistive switching at narrow operating variations, e.g., 500 ± 48 and 845 ± 39 mV. Our work offers a new approach to minimize the cycle-to-cycle stochasticity of the memristive device, paving the way for its applications in data storage and brain-inspired computing.

Folic acid relieves bone cancer pain by downregulating P2X2/3 receptors in rats

BackgroundBone cancer pain (BCP) remains a clinical challenge due to the limited and side effects of therapeutic methods. Folic acid has been known as an FDA approved dietary supplement and proved to have an analgesic effect in neuropathic pain. Here we investigate the role and mechanism of folic acid in bone cancer pain of a rat model. MethodsWalker 256 tumor cells were inoculated into the left tibia of rats to induce bone cancer pain model. Pain reflex were assessed by paw withdrawal threshold (PWT) response to Von Frey filaments and paw withdrawal latency (PWL) response to thermal stimulation. Folic acid was injected intraperitoneally to evaluate its analgesic effect in rats with bone cancer pain. Western blotting and qPCR were used to determine P2X2/3 receptor protein and mRNA levels in ipsilateral L4-6 dorsal root ganglion (DRG) and spinal dorsal horn (SDH). ResultsThe PWT and PWL of rats with bone cancer pain were obviously decreased compared to the naïve and sham rats. Interestingly, continuous folic acid treatment significantly increased the PWT and PWL of rats with bone cancer pain. P2X2 and P2X3 receptors were clearly upregulated at both mRNA and protein expression in L4-6 DRG and SDH of rats with bone cancer pain. P2X2 and P2X3 receptors were mainly localized with CGRP (calcitonin gene-related peptide) or IB4 (isolectin B4) positive neurons in L4-6 DRG of rats with bone cancer pain. Notably, continuous folic acid treatment significantly reduced the expression of P2X2 and P2X3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Finally, intrathecal injection of A317491 (a selective antagonist of P2X2/3 receptors) markedly elevated the PWT and PWL of rats with bone cancer pain. ConclusionThese results suggest that folic acid has an effective antinociceptive effect on bone cancer pain, which is mediated by downregulating P2X2/3 receptors in L4-6 DRG and SDH of rats with bone cancer pain. Folic acid may be a novel therapeutic strategy in cancer patients for pain relief.

Flexible photonic composites with responsive information display based on optical path control

Artificial photonic composites can exhibit the structural colors of embedded colloidal arrays and the functionality of polymeric matrix, making them suitable for emerging applications, such as display, sensing, and information security. However, their ability to integrate flexibility, color visibility and stimulus-responsive macroscopic change has not been adequately harnessed. In this study, a self-supporting photonic composite film is constructed by incorporating isotropically arranged polysulfide nanoparticles (PSNs) into polyethylene glycol diacrylate and butyl acrylate copolymers. The inherent light-absorbing properties and high refractive index of PSNs impart the photonic composites with high structural color visibility. Furthermore, the flexible copolymer network enables sustainable optical capability of photonic pattern during deformation. Due to its unique hydrophobic and hydrophilic structures, tunable optical path control can be achieved through solvent-induced microphase separation and inverse transparency changes under thermal stimulation, resulting in self-encryption and decryption. The function of switching desired stimuli into visualized optical signals allows flexible photonic composites to present great prospects in smart materials for dynamic memory devices.

Thermal Stimulation of Reverse Breakdown in CIGS Solar Cells

The underlying mechanisms of the initial stages of hot-spot and therefore defect creation due to reverse breakdown in Cu(In,Ga)S <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$\text{e}_{2}$</tex-math></inline-formula> solar cells are not well understood. We test the thesis, that permanent damage is created due to a positive feedback loop of local temperature enhancing the local current and vice versa, resulting in a thermal runaway. We present experiments on reverse stress with simultaneously introducing local heat. Depending on the temperature profile of the introduced heat, the local current density is enhanced and leads to a gain in the local temperature. This feedback loop is shown to lead to reverse breakdown, causing permanent damage.

Semi-Automated Recording of Facial Sensitivity in Rat Demonstrates Antinociceptive Effects of the Anti-CGRP Antibody Fremanezumab.

Migraine pain is frequently accompanied by cranial hyperalgesia and allodynia. Calcitonin gene-related peptide (CGRP) is implicated in migraine pathophysiology but its role in facial hypersensitivity is not entirely clear. In this study, we investigated if the anti-CGRP monoclonal antibody fremanezumab, which is therapeutically used in chronic and episodic migraines, can modify facial sensitivity recorded by a semi-automatic system. Rats of both sexes primed to drink from a sweet source had to pass a noxious mechanical or heat barrier to reach the source. Under these experimental conditions, animals of all groups tended to drink longer and more when they had received a subcutaneous injection of 30 mg/kg fremanezumab compared to control animals injected with an isotype control antibody 12-13 days prior to testing, but this was significant only for females. In conclusion, anti-CGRP antibody, fremanezumab, reduces facial sensitivity to noxious mechanical and thermal stimulation for more than one week, especially in female rats. Anti-CGRP antibodies may reduce not only headache but also cranial sensitivity in migraineurs.

Dual-Responsive Triple-Synergistic Fe-MOF for Tumor Theranostics.

The intelligent responsive drug delivery system has great application potential in cancer precision therapy. Although many antitumor methods have been developed based on drug delivery systems, most of them yet suffer from poor antitumor efficiency. In this project, a near-infrared and pH dual-response multimodal collaborative platform for diagnosis and treatment (PCN-DOX@PDA) was constructed. We used PCN-600 as a vehicle loaded with antineoplastic drugs and polydopamine (PDA). Under 633 nm laser irradiation, the ligand tetrakis(4-carboxyphenyl)porphyrin (TCPP) in PCN-600 can generate singlet oxygen (1O2) and kill tumor cells. PDA is used as photothermal agent of PTT. PCN-DOX@PDA achieves the intelligent release of antitumor drugs by responding to the weak acidity of the tumor microenvironment and thermal stimulation generated by NIR irradiation. In addition, since the central ion of PCN is Fe3+, PCN-DOX@PDA realizes the diagnosis and treatment of tumors through magnetic resonance imaging-mediated tumor chemotherapy and photothermal and photodynamic synergistic therapy. This triple synergistic strategy showed excellent biocompatibility and antitumor ability in in vivo experiments on a 4T1 tumor-bearing mouse model, indicating that PCN-DOX@PDA has a good development prospect in the field of precision cancer therapy and diversified biomedical applications.

Mitigating Lactate-Associated Immunosuppression against Intracellular Bacteria Using Thermoresponsive Nanoparticles for Septic Arthritis Therapy.

Intracellular bacteria are the major contributor to the intractability of septic arthritis, which are sequestered in macrophages to undermine the innate immune response and avoid the antibacterial effect of antibiotics due to the obstruction of the cell membrane. Herein, we report a thermoresponsive nanoparticle, which consists of a phase-change material shell (fatty acids) and an oxygen-producing core (CaO2-vancomycin). Under external thermal stimulation, the shell of the nanoparticle transforms from a solid phase to a liquid phase. Then the CaO2-Vancomycin core is exposed to the surrounding aqueous solution to release vancomycin and generate Ca(OH)2 and oxygen, thereby depleting accumulated lactate to mitigate lactate-associated immunosuppression, stabilizing hypoxia-inducible factor-1α (HIF-1α) to enhance M1-like polarization of macrophages, and increasing reactive oxygen species (ROS) and reactive nitrogen species (RNS) production. This combined effect between the controlled release of antibiotics and enhancement of host innate immunity provides a promising strategy to combat intracellular bacteria for septic arthritis therapy.

Biomimetic 4D printing of dome-shaped dynamic mechanical metamaterials

The dome shaped structures of natural organisms can provide design inspiration for impact resistant structural materials. Most of the existing biomimetic dome shaped impact resistant materials are directly formed using 3D printing technology, and the samples cannot dynamically adjust the impact resistance properties in the various applications. Herein, 4D printing of fused filament fabrication is employed to form flat structures with different structural patterns via programming the printing process parameters, which can be deformed into biomimetic dome structures with dynamic thermal stimulation treatment. The thermal stimulation treatment can drive the dynamic transformation of 2D planar structures into 3D structures, and the higher the thermal stimulation temperature, the greater the degree of eminence and the denser the structure under the same experimental conditions. The thermal stimulation temperature and the mechanical properties of the structural samples show a certain positive correlation. In addition, different design structural patterns have different compressive mechanical properties. The more the number of circular units, the more obvious the energy absorption characteristics. The impact-resistant biomimetic structures can be applied to the surface strengthening and cushioning of various weapons, vehicles, heavy equipment, and launch structure.

Characteristics of CO2 hydrate formation and dissociation at different CO2-water ratios in a porous medium

Hydrate-based CO2 sequestration (HBCS) is a new concept to store a huge amount of CO2 in geological sediments in the form of crystalline solids. This technique can address several environmental challenges of the current geologic carbon sequestration approaches. This study aims to evaluate and compare the characteristics and kinetics of CO2 hydrate formation and dissociation in the porous medium (three-phase) and bulk (two-phase) conditions at different CO2-water ratios. The experimental results indicate that the pressure drops and consequently hydrate nucleation and growth are limited in the porous medium due to the presence of solid particles, limited CO2 mass transfer, and heat transfer. Calculation of CO2 mass consumption and rate of gas uptake during hydrate formation also support the limited CO2 consumption and gas uptake rate in the porous medium, compared to the bulk condition. The calculated driving force for each test condition suggests its critical importance in the kinetics of CO2 hydrate formation. On the other hand, limited CO2 diffusion to the aqueous phase, hydrate nucleation at the solid-CO2-water interface, and better protection of newly formed hydrates are among the main reasons for the success of hydrate formation at the CO2-limited ratio only in the porous medium. Lastly, three steps during hydrate dissociation via thermal stimulation, such as heat transfer driving, kinetic driving, and fluid flow driving, are no longer discernable in the porous medium due to the limited heat and mass transfer during the process.

Effect of thermal oxidant stimulation on the alteration of shale seepage channel: Implication from the change of macromorphology and microstructure

Alteration of the seepage channel through the shale-oxidant interactions is critical for using oxidant stimulation to enhance fluid mobility in tight shale reservoirs. Although many studies reported the effect of oxidant stimulation on the macro-crack morphology of shale, little is still known about its impact on shale microstructure, especially under reservoir temperature. In this study, we explored the influence of three oxidants, including H2O2, NaClO and Na2S2O8, on the alteration of shale pores and fractures at reservoir temperature (60 °C), and illustrated the underlying mechanism based on water chemistry and solid characterization including X-ray diffraction, Fourier-transform infrared spectroscopy, low-pressure nitrogen gas adsorption, scanning electron microscopy coupled with energy dispersive spectroscopy analyses. Results showed that the reaction temperature and oxidant type both led to an inconsistent response of shale macromorphology and microstructure during oxidant stimulation. Three oxidants processed different shale matrix alteration, with the degree shown as follows: Na2S2O8 > H2O2 > NaClO. Even though gas generated from the thermal and catalytic decomposition of H2O2 and dissolution of carbonate were both favorable for the shale plug disintegration, the rapid consumption of H2O2 limited the change of shale pore structure. After being treated with NaClO, the organic nanopores were transformed into larger mesopores and macropores with a decrease in pore surface roughness and structural complexity. Therefore, other methods should be combined with NaClO simulation to generate fractures and improve the fluid fluidity of shale. The serious dissolution and precipitation of minerals by Na2S2O8 causes significant alteration in the shale matrix, resulting in a large number of pores and induced fractures. Our research confirms the distinct effects of different oxidants on shale fractures and micropores, and the most promising Na2S2O8 for improving shale permeability, but still needs to be demonstrated by core-flooding experiments in the future.

Relaxation behavior at high temperature associated with oxygen vacancy in the Sr1-x(LiCe)x/2.5Bi2Nb2O9 ceramics

Dielectric relaxation behavior and conductiviy of Sr1-x(LiCe)x/2.5Bi2Nb2O9 ceramics prepared by conventional solid-sate reaction method were investigated. With the introduction of the (LiCe), the conductivity was improved significantly and the large conductivity at high temperature (∼10−4 S·cm−1 at 530 °C) could be obtained. Phase transition from normal ferroelectric to relaxor-like ferroelectric was detected in the Sr1-x(LiCe)x/2.5Bi2Nb2O9 ceramics. Results of the complex impedance analysis indicated that the concentration of charge carriers increased gradually with the increase in (LiCe). Oxygen vacancy was confirmed to be responsible for the conductivity at high temperature by calculating activation energy of the carriers in the corresponding range of high temperature. Dielectric relaxation behavior at high temperature was attributed to the short-range conductivity of oxygen vacancies under thermal stimulation.

The pro-inflammatory cytokine IL-Iβ alteration by deer (Rusa unicolor) antler extract on osteoarthritis rat model

Osteoarthritis is a disease associated with articular cartilage degradation, intra-articular area inflammation, and subchondral bone replacement. Cytokine IL-1β has a prominent function in the inflammations process that passes in the joints. The 70% ethanol extracts of deer antler (250 and 500 mg/kg BW) and glucosamine sulfate (250 kg/BW) were evaluated for four weeks in reducing cytokine IL-1β to rat model OA-induced Monosodium iodoacetate. Measurements of joint diameter in rat’s knee and hyperalgesia were performed on weeks 0, 1, 2, 3, 4, 5, 6, and 7. The presence of a significant difference in the stimulation thermal latency (p = 0.00) and the resulting increase in swelling of joint diameter (p = 0.00) are evidence that MIA has successfully induced the rat modeling of OA. A significant decrease in cytokine IL-Iβ levels was shown on week 3 after MIA injection (p = 0.00). Both concentrations of deer extracts significantly reduced knee joint diameter (p = 0.00), latency thermal stimulation (p = 0.00), and cytokine IL-1β levels (p = 0.00). Based on the results, it can be concluded that the 70% ethanol extract of deer antler is a potential medicine for OA therapy.

Roles of montmorillonite clay on the kinetics and morphology of CO2 hydrate in hydrate-based CO2 sequestration

Hydrate-based CO2 sequestration in marine sediments has emerged as a novel sustainable technology for long-term and stable CO2 sequestration. However, the role of clay minerals in CO2 hydrate formation and dissociation in clay-rich sediments, which are widely distributed in the South China Sea, remains controversial due to a lack of experimental evidence. This study investigates the effect of a representative clay mineral, sodium montmorillonite (Na-MMT), on the nucleation and growth kinetics of CO2 hydrate in suspensions with a msass fraction below 20.0 wt%. The kinetic experiments reveal that Na-MMT significantly reduces the induction time due to the additional nucleation sites provided by the delamination of clay particles and the induced surface electric field. While the average growth rate of CO2 hydrate is reduced by ∼72 % for Na-MMT mass fraction above 5.0 wt%. Morphologically, hydrate-clay stratification and gas-tunneling behaviors were observed, providing explanations for the retarded kinetics. The mass transfer of CO2 from the gas phase to the liquid phase is impeded by the high viscosity of the suspension and the clay-induced strongly-polarized water layer, which retards the overall kinetics of CO2 hydrate formation. Upon thermal stimulation, partial CO2 hydrate dissociation was observed within the pure CO2 hydrate stability region for 10.0 wt% Na-MMT suspension, indicating a possible impact of Na-MMT on CO2 hydrate thermodynamics. Moreover, the addition of Na-MMT clay promotes CO2 hydrate dissociation kinetics significantly. This study provides fundamental insights into the interaction between Na-MMT clay and CO2 hydrate, offering new perspectives for designing effective strategies for CO2 sequestration in abundant clay-rich marine sediments.