Thermal Extraction Method Research Articles

A solvent-free thermal desorption-gas chromatography-tandem mass spectrometry method (TD-GC-MS/MS) for the determination of polycyclic aromatic hydrocarbons in gas phase airborne samples.

A solvent-free, thermal extraction method for analysis of polycyclic aromatic hydrocarbons (PAHs) in gas phase airborne samples was developed. A fully automated thermal desorber (TD) coupled with highly selective and sensitive gas chromatography-tandem mass spectrometry (GC-MS/MS) was used to determine the concentration of trace level PAHs. Air sampling was conducted to tune the sampling and analytical conditions. Various instrument operating parameters such as sorbent tube desorption temperature/time, cold trap desorption temperature/time, outlet split ratio, tube storage stability, as well as air sampling flow rate and time were tested to optimize the analytical conditions. Method performance showed linearity in broad range (0.01 to 10 ng) with regression coefficients of external calibration curves (R2) >0.998 for all targeted PAHs. Method detection limit (MDL) was between 0.01-0.05 ng per tube. The method precision (<20 %) and accuracy were also satisfactory, obtaining quantitative recoveries (mean values between 80 and 120 %). The method has been applied for both outdoor and indoor air analysis. Small volume of air sample (<144 L) was sufficient for the PAH analysis. Naphthalene, 1-methylnaphthalene, 2-methylnaphthalene, anthracene, fluoranthene, acenaphthene, fluorene, pyrene, and acenaphthylene are the primary PAHs in the gas phase for both indoor and outdoor air. Naphthalene and its two methylated compounds: 1-methylnaphthalene and 2-methylnaphthalene accounted for 47.7 % and 81.7 % of the total gas phase PAHs for outdoor and indoor, respectively. Using thermal extraction instead of organic solvent extraction for sample pretreatment makes the method sustainable and in consonance with the principles of green chemistry. No solvent and time-consuming extract step are needed. The method greatly improved the analytical process with a fully automated TD-GC-MS/MS instrument.

Revealing the off-flavors in hydro-distilled essential oils of sweet orange (Citrus sinensis) by flavoromics strategy and computational simulation

Citrus essential oils (EOs) are renowned for their aroma, but thermal extraction methods can introduce undesirable cooking and pungent odors. The specific compounds responsible for these off-flavors and their perception mechanisms remain unclear. This study investigated the flavor differences between cold pressing (CP) and hydro-distilled (HD) extracted essential oils from sweet oranges (SEOs) using flavoromics strategy. The results indicated that increased levels of alcohol compounds, particularly terpinen-4-ol, β-citronellol, geraniol, trans-carveol, and α-terpineol, were primarily responsible for the off-flavors in HD-extracted SEOs. Furthermore, molecular docking and dynamics simulations revealed that off-flavor compounds bind to olfactory receptors through hydrogen bonding and hydrophobic interactions, inducing slight structural and conformational changes that facilitate odor recognition and activate downstream signaling events. This study offers crucial insights into the mechanisms underlying off-flavor development in citrus EOs, providing valuable knowledge for optimizing extraction methods, controlling SEOs quality, and advancing flavor science.

Investigation of tribological behavior of poly(methyl methacrylate) biocomposite containing hydroxyapatite

Poly(methyl methacrylate) (PMMA), is widely used in biomedical applications such as bone cement. However, it is known to have low wear resistance. To enhance its tribological behavior, a reinforcement phase is necessary. For this purpose, in this study, homemade hydroxyapatite (HAp) was utilized to enhance the mechanical properties and wear resistance of PMMA while also increasing its biocompatibility. Using the thermal extraction method, natural raw materials were used to synthesize homemade HAp powder successfully. The d 0.5 of HAp powders in particle size analysis was 7.464 μm. Hot isostatic pressing (HIP) was used to create PMMA–HAp biocomposites with various HAp ratios (5, 10 and 15 wt%) in PMMA. The wear morphology was characterized by scanning electron microscopy (SEM). The SEM images showed a severe abrasive wear mechanism, but a relatively stable wear surface was observed that indicated slight abrasion during the wear tests with the increase in HAp ratio. The highest wear rate was obtained in 15% HAp-reinforced PMMA at a sliding speed of 1.0 m/s and had value of 4.72 × 10−4 mm3/(N m) under a 20 N load. The HIP’ed densities of the samples increased from 1.15 to 1.31 g/cm3 with increasing amount of HAp. Similarly, the Vickers test results showed that the hardness values increased from 14.76 to 22.03 HV.

Evaluation of cold extracted bioactive compounds from tomato pomace and its use in food fortification

ABSTRACT The purpose of this study was to find a method for the cold extraction of biologically active compounds from tomato pomace, as there are no acceptable, affordable and environmentally friendly extraction methods, and its ability to maintain their vital functions and properties. This method was then evaluated as compared to the traditional thermal extraction methods. The limits of traditional extraction techniques have been overcome by the advent of numerous non-thermal new extraction techniques over the past 20 years. Three methods were used to help extract these compounds, which are blending the frozen (BF), microwave-assisted (MA) and Ohmic heating (OH). The results showed that the BF method was the best extraction method, and the amount of lycopene 487.4 µg/g, β-carotene 115.8 µg/g, total phenolics 1297.4 µg GAE/g, total flavonoids 462.5 µg QE/g and antioxidant activity 89.1%. It was found that the most abundant phenolic compounds in tomato pomace were gallic acid and ellagic nacid, as it contains seven essential amino acids, and that glutamic acid was the dominant acid. Fatty acid estimation showed that linoleic cis and oleic cis were the dominant acids, as they were 585.50 and 164.67 µg/g total fatty acids, respectively. It also contained on unsaturated fatty acids 820.44 µg/g.

Influence of High-Power Ultrasound on Yield of Proteins and Specialized Plant Metabolites from Sugar Beet Leaves (Beta vulgaris subsp. vulgaris var. altissima)

Ultrasound with water as a green solvent is an effective strategy for reducing losses and increasing the utilization of by-products. The extraction of proteins and specialized plant metabolites from sugar beet leaves (Beta vulgaris subsp. vulgaris var. altissima) promotes sustainability in the agro-food chain. Guided by sustainability, samples treated with ultrasound showed lower energy consumption and lower CO2 emissions. Furthermore, the spectrophotometric determination revealed higher protein and phenol yields in ultrasonically treated samples compared to thermally treated ones. The highest yield of total proteins, 147.91 ± 4.58 mg (gd.m.)−1, was observed during ultrasound treatment (amplitude 100%, treatment time 9 min). Under the same extraction conditions, the same trend was observed in the yield of total phenols 17.89 ± 0.38 mg (gd.m.)−1. High-power ultrasound, compared to the thermal extraction method, has increased the yield of proteins and specialized plant metabolites with significantly lower energy consumption and CO2 emissions. The obtained results are in accordance with the foundations of sustainable development. From an economic and environmental point of view, ultrasound with the use of green solvents would be an excellent replacement for conventional extraction methods.

Determination of in situ hydrocarbon contents in shale oil plays. Part 2: Two-dimensional nuclear magnetic resonance (2D NMR) as a potential approach to characterize preserved cores

Determination of the in situ hydrocarbon content and occurrence characteristics of shale oil reservoirs is crucial for reserve prediction and efficient development. Although many scholars have studied the fluid saturation of conventional coring shale using solvent and thermal extraction methods, with time consuming and only volumes or mass of oil and water were given, which could not reveal the occurrence characteristics of oil and water in samples. This study proposes a two-dimensional (2D) nuclear magnetic resonance (NMR) T1–T2 mapping method to systematically assess the in situ water and oil content, distribution, and their evaporative loss laws of preserved shales. Sixty preserved shales with different maturities were selected from the first member of the Qingshankou Formation in the northern Songliao Basin, China. Each preserved sample was divided into two parts and analyzed using a high-frequency (22 MHz) NMR and Dean–Stark extraction respectively. Furthermore, a time-elapsed NMR T1–T2 mapping was performed on a preserved shale core sample after varying the exposure times in an open environment. Results show that T1–T2 mapping can be used to determine the porosity, water and oil contents, and saturations of preserved shales. Oil content measured using T1–T2 mapping was consistent with that obtained by Dean–Stark extraction, with a correlation coefficient (R2) as high as 90% when crude oils of varying maturities were used for NMR calibration.The T1/T2 of oil inpreserved shale showed a negative trend with maturity, and the T2 of oil was often higher than that of water, indicating oil is enriched in the relative macropores. After exposing the preserved shale to open air for 150–200 h, 1) the total loss ratio of oil and water was approximately 80%; 2)the fluid evaporation loss rate follows the orders: oil > water and fluids in larger pores > smaller pores; 3)the free oil content decreased by approximately 92%, whereas the absorbed oil content varied slightly. As a nondestructive, rapid, and minimal sample preparation method, 2D NMR can efficiently assess both fluids content and distribution of a core sample in only 5 min, thus displaying its great advantages and applications for preserved shales with fast fluid evaporation to determine the in situ fluid content.

Optimized structural parameters and heat extraction capacity of a mixing device for constant pressure CO2 mineralization using alkaline waste.

Alkaline waste such as calcium carbide slag is an ideal material for mineralizing CO2 and promoting atmospheric carbon reduction. In this study, the structural parameters of a mixing device and a thermal extraction method for the high-efficiency mineralization of CO2 using alkaline waste were optimized. First, the influence of structural parameters was studied by means of numerical simulation, and it was found that when the length-diameter ratio, blade angle, spacing, and diameter of the mixing device were 3, 15, 6 cm, and 14 cm respectively, 2.14 t CO2 can be mineralized within 1 h. The amount of heat extracted from mineralization of 1 t CO2 reached 189.60 MJ. In addition, the winding configuration of the heat pipe, which is beneficial for extracting more reaction heat, was optimal, and a model of the relationship between the heat pipe outlet water temperature and flow velocity at the outlet of the heat pipe was established. This study provides theoretical guidance for the field application of alkaline waste for high-efficiency mineralization of CO2, which can accelerate the realization of peak CO2 emissions and carbon neutrality.

Transformation Mechanism of Rare Ginsenosides in American Ginseng by Different Processing Methods and Antitumour Effects

The mechanism by which ginsenosides from Panax quinquefolium L. transform into rare saponins by different processing methods and their antitumour effects have yet to be fully elucidated. Our study aimed to detect the effect of amino acids and processing methods on the conversion of ginsenosides in American ginseng to rare ginsenosides, using 8 monomeric ginsenosides as substrates to discuss the reaction pathway and mechanism. S180 tumour-bearing mice were established to study the antitumour effects of American ginseng total saponins (AGS-Q) or American ginseng total saponins after transformation (AGS-H) synergistic CTX. The results showed that aspartic acid was the best catalyst, and the thermal extraction method had the best effect. Under the optimal conditions, including a reaction temperature of 110°C, an aspartic acid concentration of 5%, a reaction time of 2.5 h and a liquid-solid ratio of 30 mL/g, the highest conversion of Rk1 and Rg5 was 6.58 ± 0.11 mg/g and 3.74 ± 0.05 mg/g, respectively. In the reaction pathway, the diol group saponins participated in the transformation process, and the triol group saponins basically did not participate in the transformation process. AGS-Q or AGS-H synergistic CTX, or AGS-H synergistic CTX/2 could significantly increase the tumour inhibition rate, spleen index and white blood cell count, had a significant upregulation effect on IL-2 and IL-10 immune cytokines; significantly restored the ratio of CD4+/CD8+; and significantly inhibited the level of CD4+CD25+. AGS-Q or AGS-H synergistic with CTX or CTX/2 can significantly upregulate the expression of Bax and cleaved-Caspase-3 and inhibit the expression of antiapoptotic protein Bcl-2. AGS synergistic CTX in the treatment of S180 tumour-bearing mice can improve the efficacy and reduce toxicity.

Alteration of Physico-mechanical Properties of Black Tilapia Scale Gelatins using UVA and UVC Irradiation

Fish gelatin is abundant, relatively low cost and biodegradable. However, their inferior mechanical and rheological properties make them less competitive compared to mammalian gelatins. Hence, the effects of Ultraviolet (UV) irradiation on the properties of scale gelatins were analyzed. Gelatins were extracted from black tilapia scale via thermal extraction method. The gelatins were then subjected to Ultraviolet-A (UVA) and Ultraviolet-C (UVC) irradiation for 0.5 to 2.5 h and the changes in gel strength, viscosity, and melting temperature were observed. Results obtained show a marked increase in the gel strength and viscosity of the gelatins. However, the effects on the melting temperature are minimal. Treatment with UVA and UVC improved the gel strength of the gelatins up to 5.12 ± 0.22 N and 4.75 ± 0.09 N, respectively. Further analysis using Fourier Transform Infrared (FTIR) Spectroscopy showed crosslinking formation in the polypeptide chains induced by UV irradiation. UVA was found to be more effective in enhancing the properties of scale gelatins compared to UVC. In general, UV-irradiated scale gelatins showed excellent properties compared to the commercial bovine gelatin. Results indicated the prospects of employing UV treatment in enhancing the properties of fish gelatin.

Preparation of hot-pressed coal briquette with the extract from direct coal liquefaction residue

Direct coal liquefaction residue (DCLR) is an industrial by-product from the direct coal liquefaction process. It is harmful to the environment and the human body when directly charged or used due to the high content of sulfur, ash, and polycyclic aromatic hydrocarbons (PAHs). Therefore, it is of great importance to treat and utilize DCLR. In this paper, the thermal extraction method was applied to extract DCLR with N-methylpyrrolidone (NMP), and the hot-pressed coal briquette (HPCB) was prepared with DCLR extract and anthracite powder. DCLR and its extract and residue were characterized by TG, FTIR, and GC-MS. The ash content was decreased from 11.72% to 0.72% and the sulfur content was decreased from 1.72% to 0.09% after thermal extraction. TG analysis shows that the weight loss of the extract was about 30.4% higher than that of the residue during the pyrolysis process, indicating that the extract contained more light components. FTIR analysis shows that the thermal extraction process had strong extraction capacity for aliphatic hydrocarbons and aromatic hydrocarbons, but weak extraction capacity for carboxyl, phenol, or alcohol groups. GCMS shows that 60.6% of the volatile substances in the extract were PAHs, while the residue had only 6.8% of PAHS. It is proven that the residue has been rendered harmless to humans and the environment. Moreover, the HPCB with a compressive strength exceeding 2.5 MPa was prepared by using the DCLR extract as a binder and anthracite powder as a raw material. It has the potential and advantages of being used as a metallurgical raw material.

Optimization of Microwave-Assisted Extraction of Palm Kernel Cake Protein

Palm kernel cake (PKC) is an abundant by-product of the palm oil industry. It is used as an ingredient in feed due to the high amount of protein and fiber content. In order to increase the value of PKC, the PKC protein can be extracted and may be able to be used as an alternative protein for plant-based food. This study aims to optimize the PKC protein extraction using the microwave-assisted extraction (MAE) method with a response surface methodology (RSM). MAE is a green extraction method due to less chemicals needed, less time and less energy consumption when compared to the traditional thermal extraction method. The experiment was designed by the Box-Behnken method with 3 factors; microwave power (A), extraction time (B) and solid-liquid ratio (C). The optimum condition was at the microwave power of 700.16 W, extraction time of 543.08 s and the solid-liquid ratio of 1:7.73 g PKC/ ml water resulting in a theoretical yield of protein extraction of 32.46%.

Emergent Technologies for the Extraction of Antioxidants from Prickly Pear Peel and Their Antimicrobial Activity.

Phenolic compounds are important bioactive compounds identified in prickly pear peel that have important antioxidant and antimicrobial properties. However, conventional thermal extraction methods may reduce their bioactivity, and technologies such as high pressure (HP) and ohmic heating (OH) may help preserve them. In this study, both technologies were analyzed, individually and combined (250/500 MPa; 40/70 °C; ethanol concentration 30/70%), and compared with Soxhlet with regard to total phenolics, flavonoids, and carotenoids as well as antioxidant (ABTS, DPPH, ORAC), DNA pro-oxidant, and antimicrobial (inhibition halos, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curves, and viable cells) activities of prickly pear peel extracts. Total phenolics extracted by each technology increased 103% (OH) and 98% (HP) with regard to Soxhlet, but the contents of total flavonoids and carotenoids were similar. Antioxidant activity increased with HP and OH (between 35% and 63%), and OH (70 °C) did not induce DNA degradation. The phenolic compound present in higher amounts was piscidic acid, followed by eucomic acid and citrate. In general, their extraction was significantly favored by HP and OH. Antimicrobial activity against 7 types of bacteria showed effective results only against S. aureus, S. enteritidis, and B. cereus. No synergetic or additive effect was observed for HP/OH.

Effect of ultrasound-assisted cold plasma pretreatment to obtain sea asparagus extract and its application in Italian salami

The effect of the glow discharge cold plasma pretreatment (CPT) was investigated on the ultrasound-assisted extraction (UAE) of phenolic compounds from sea asparagus Salicornia neei. The extract was applied in dry fermented sausage (Italian salami). Thermal extraction methods demand a long processing time, are highly energy-consuming and cause irreversible nutrient losses. It was found that CPT (discharge power of 14 W for 5 min) prior to UAE increased the antioxidant activity by 22% and 19% measured by the DPPH and ABTS assays, respectively. The S. neei extract showed high antioxidant activity, low antimicrobial activity, and was added to salami formulations with reduced-sodium nitrite content and no addition of sodium erythorbate. Despite a slight color change, lipid oxidation and texture parameters were similar to the control at the end of ripening. Furthermore, higher antioxidant activity was observed in S. neei extract supplemented salami with no impact on its sensory overall acceptability, indicating its potential as a natural alternative to synthetic additives.

ATR-MIR Spectroscopy Predicts Total Phenolics and Colour for Extracts Produced by Microwave-Assisted or Conventional Thermal Extraction Methods Applied Separately to Mixtures of Grape Skins from White or Red Commercial Cultivars

Attenuated total reflectance mid-infrared (ATR-MIR) spectra were collected from 480 grape skin extracts produced in a wide range of conditions. The conditions were created at different phases in parallel response surface RS-optimisation experiments aiming to maximise the total phenolics in red and white grape skin extractions by microwave-assisted (MAE) or conventional thermal (CTE) methods. Skins mixtures for six white and six red Australian commercial cultivars were prepared from grapes collected at veraison and harvest. The total phenolics were measured for individual extracts with the Folin-Ciocalteu method. Partial least squares (PLS1) regression was employed on the spectra to predict the total phenolics (TP) and colour (CIELAB chroma C*) of individual extracts. Models based on the raw spectra and their second derivative (Savitzky-Golay, 20 points) were examined using the full range of spectra 4000–400 cm−1, and sub-ranges: 4000–1100 cm−1, 1500–400 cm−1, 1500–1100 cm−1 and 1457–1168 cm−1. The PLS1 models based on the second derivative of the range of 4000–1100 cm−1 provided the best results for both TP and C*, for each skin colour. Overall, the residual predictive deviation (RPD) exceeded 3.0, indicating the PLS1 models’ ability for fair classification, suitable for screening. A comparative analysis was performed for the range of 4000–1100 cm−1 between the spectra of white and red skin mixture extracts: the peaks at 3840 cm−1, 2940 cm−1, 2358 cm−1, 2197 cm−1, 2136 cm−1, 1725 cm−1, 1100 cm−1 and 1148 cm−1 are flagged to be investigated in further research. ATR-MIR spectroscopy can reduce the time and costs of TP and C* analyses in grape skin extracts.

Evaluating the total oil yield using a single routine Rock-Eval experiment on as-received shales

Shales in the early maturity and oil window stages contain a considerable amount of heavy hydrocarbons (S2oil) having strong interactions with kerogen/rock that make the accurate measurement of total oil (total extractable organic matter) more difficult from the routine Rock-Eval experiment. In this study, a fast method for evaluating the total oil yield using a single routine Rock-Eval experiment on as-received shales is proposed. First, the temperature threshold (TOK) of the S2oil and cracking hydrocarbons were determined by combining the pyrograms of the as-received shale with a solvent-extracted replicate. Then, the total oil yield was directly derived from the hydrocarbons evaporate at a temperature below than TOK in a routine Rock-Eval experiment. The results show that the TOK value is controlled by the sample’s maturity and pore structure. The higher the maturity, the larger the specific surface area and the smaller the pore size, the greater the TOK. A prediction model of TOK was proposed based on the sample’s production index (PI). The total oil yields estimated by the two methods of both the TOK prediction model and the average TOK value (465 °C) are consistent with those obtained by Jarvie (2012) using the thermal-extraction method with correlation coefficients of 0.983 and 0.9548, respectively. Compared with the previous methods, the single routine Rock-Eval experiment method proposed in this study is convenient and not requires an extraction experiment. In addition, there are archived routine pyrolysis data available that can be used to directly calculate the total oil yield based on the temperature threshold.

1-13 逐次的抽出法を利用した褐炭のフラクショネーション((5)化学構造,溶剤抽出,灰,Session 1 石炭・重質油等,研究発表(口頭発表))

1-13 逐次的抽出法を利用した褐炭のフラクショネーション((5)化学構造,溶剤抽出,灰,Session 1 石炭・重質油等,研究発表(口頭発表))

Extraction of pectin from the peels of pomelo by high-speed shearing homogenization and its characteristics

High-speed shearing homogenization extraction (HSHE) was applied to extract pectin from the peels of pomelo using a high-speed shearing extractor for the first time and the extraction conditions were optimized using response surface methodology with Box-Behnken design. The optimal extraction conditions were determined: pH of extraction solvent 1.24, extraction voltage 156 V, extraction time 240 s, with the predicted extraction yield was 209 g/kg, which was very consistent with the experimental value (209 ± 2 g/kg) and significantly higher than traditional thermal extraction method (175 ± 6 g/kg at 85 °C for 80 min). Furthermore, pectin extracted by HSHE has a higher viscosity, intrinsic viscosity and viscosity-average molecular weight than traditional thermal extracted pectins. The results demonstrated that HSHE has a great potential for the extraction of pectin with higher viscosity efficiently.

Recovery of protein from green leaves: Overview of crucial steps for utilisation

Plant leaves are a major potential source of novel food proteins. Till now, leaf protein extraction methods mainly focus on the extraction of soluble proteins, like rubisco protein, leaving more than half of all protein unextracted. Here, we report on the total protein extraction from sugar beet leaves (Beta vulgaris L.) by a traditional thermal extraction method consisting of mechanical pressing, heating to 50°C and centrifugation. The resulting streams (i.e. supernatant, green-protein pellet and fibrous pulp) were characterised in terms of composition, physical structure and processing options. The protein distributed almost equally over the supernatant, pellet and pulp. This shows that thermal precipitation is an unselective process with respect to fractionation between soluble (rubisco) and insoluble (other) proteins. About 6% of the total protein could be extracted as pure rubisco (90% purity) from the supernatant. Surfactants commonly used for protein solubilisation could hardly re-dissolve the precipitated proteins in the pellet phase, which suggested that irreversible association was induced between the co-precipitated proteins and cell debris. Thus, the extraction of this protein will require prevention of their co-precipitation, and should take place in the original juice solution.

Evaluation of the extracellular polymeric substances by confocal laser scanning microscopy in conventional activated sludge and advanced membrane bioreactors treating hospital wastewater.

Confocal laser scanning microscopy (CLSM) combined with fluorescent viability indicators, was used in this study to investigate the impact of hospital wastewaters on floc structure and composition. In this work, three pilot-scale projects, two membrane bioreactors (MBRs) with a submerged or external membrane bioreactor and a conventional activated sludge, were installed and operated for 65 days. They were fed with an influent sampled directly from the hospital drainage system, which contained micropollutant concentrations ranging from ng/L to mg/L. Samples of flocs were observed using CLSM to characterize the extracellular polymeric substances (EPS) stained with concanavalin A-tetra methylrhodamine and fluorescein isothiocyanate solution and combined with a fluorescent viability indicator (Baclight(®) Bacterial Viability Kit, Molecular Probes), allowing visualization of isolated stained cells in the three-dimensional structure of flocs (damaged or not). The results of CLSM of the sludge composition were compared with classical biochemical analysis of EPS made through a thermal extraction method. The results showed a good relation between these analyses and the statistical treatment of microscopic pictures.

Inhibition by Yeast-Derived Mannoproteins of Adherence to and Invasion of Caco-2 Cells by Campylobacter jejuni

The main objective of the present work was to study the influence of yeast-derived mannoproteins on the adherence to and invasion of Caco-2 cells by Campylobacter jejuni. Mannoprotein fractions were prepared by enzymatic and thermal extraction methods. The method used to prepare the mannoprotein extracts influenced their composition and determined the efficacy of the extract against C. jejuni adherence and/or invasion. The availability of mannose in the mannoprotein fraction seemed to be important for inhibiting effective adherence and invasion of Caco-2-cells by C. jejuni, although protein moieties also played a role in the process. The study of the mechanisms involved in the inhibition of C. jejuni adherence and invasion by mannoproteins may have further implications in the control of this foodborne pathogen.