In order to obtain the mechanical behavior of H-shaped high-strength Q960 steel column under axial compression in fire, the bearing capacity tests of two Q960 steel columns at ambient temperature and fire resistance tests of six Q960 steel columns at elevated temperature were carried out. By considering different slenderness ratios, load ratios, and heating rates of the test columns, the furnace temperature, column temperature, lateral displacement, and axial displacement were measured and recorded. Then, critical temperature, fire resistance, and failure mode of the columns were obtained. The thermal-mechanical coupling analysis model for the Q960 steel column was established by using ABAQUS finite element analysis software. After verifying the accuracy of the model, parameter analysis was carried out to investigate the effects of load ratio, slenderness ratio, geometric imperfection, thermal creep, residual stress, and steel strength on the fire resistance of steel columns. Based on the test data and analysis results, a fire resistance design method for high-strength Q960 steel columns under axial compression is proposed.