In waterfronts, thermal comfort is affected by climate and urban morphology. Existing studies on waterfront thermal comfort have mainly focused on warm and hot regions, focusing on the cooling effects of water and vegetation during the summer. The research methods are mostly questionnaire surveys and simulations, whereas the urban form and climate characteristics of cold regions are rarely considered, and the results of these studies are seldom applied to design practices. This study focused on the thermal comfort of waterfronts in cold regions. Computational Fluid Dynamics, Mean Radiant Temperature, and the Universal Thermal Climatic Index were used to examine the effects of tree–water features (winter river ice and tree defoliation) on thermal comfort in cold regions, and correlation analyses were combined to screen for relevant urban morphology factors for waterfront thermal comfort. Regression analyses were conducted to understand the influence of six factors, namely, building orientation, floor area ratio, open space ratio, building height to distance between river and building ratio, standard deviation of the first building row, and vegetation concentration, on waterfront thermal comfort. After training and comparing the four thermal comfort prediction models, a genetic algorithm combined with an artificial neural network was applied to optimize the design of the urban morphology of the six waterfront blocks. The optimization improved the waterfront thermal comfort performance by 22%, 7%, 77%, 106%, 3%, and 3%, respectively.
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