Aloe viscose fiber is rich in a variety of active ingredients, with excellent moisturizing and antibacterial properties, and gradually widely used in clothing production. Therefore, this paper focuses on the thermal comfort of aloe viscose seamless knitted clothing, 15 sets of seam knitting sets with different yarn blending ratios and knitted structures were designed. thermal manikin was used in artificial climate to test the thermal resistance and the voting rate of whole body thermal sensation. SPSS two-factor variance analysis and Duncan multiple comparison method were used to analyze the experimental data. The results show that the difference of veil material and knitted structure has an impact on clothing thermal comfort. In all the samples, the veil is made of 100:0 aloe viscose/viscose fiber, and the thermal comfort of the seam knitting garment w Abstract: In order to study the thermal comfort performance of aloe viscose fiber seamless knitted clothing, 15 sets of seam knitting sets with different yarn blending ratios and knitted structures were designed. Thermal manikin was used in artificial climate to test the thermal resistance and the voting rate of whole body thermal sensation. SPSS two-factor variance analysis and Duncan multiple comparison method were used to analyze the experimental data. The results show that the difference of veil material and knitted structure has an impact on clothing thermal comfort. In all the samples, the veil is made of 100:0 aloe viscose/viscose fiber, and the thermal comfort of the seam knitting garment with the knitted structure of 1 + 3 false rib fabric is the best, with the sets thermal resistance of 0.289 m2 K/W and the sets PMV value of −1.75. It provides theoretical basis and guiding significance for the future production of aloe seamless knitted clothing with excellent thermal resistance.