Thermal Adaptation Research Articles

Thermal adaptation in plants: understanding the dynamics of translation factors and condensates.

Plants, as sessile organisms, face the crucial challenge of adjusting growth and development with ever-changing environmental conditions. Protein synthesis is the fundamental process that enables growth of all organisms. Since elevated temperature presents a substantial threat to protein stability and function, immediate adjustments of protein synthesis rates are necessary to circumvent accumulation of proteotoxic stress and to ensure survival. This review provides an overview of the mechanisms that control translation under high-temperature stress by the modification of components of the translation machinery in plants, and compares them to yeast and metazoa. Recent research also suggests an important role for cytoplasmic biomolecular condensates, named stress granules, in these processes. Current understanding of the role of stress granules in translational regulation and of the molecular processes associated with translation that might occur within stress granules is also discussed.

Microbial communities are thermally more sensitive in warm-climate lizards compared with their cold-climate counterparts.

Environmental temperature affects the composition, structure, and function of the gut microbial communities in host animals. To elucidate the role of gut microbiota in thermal adaptation, we designed a 2 species × 3 temperatures experiment, whereby we acclimated adult males of two agamid lizard species (warm-climate Leiolepis reevesii and cold-climate Phrynocephalus przewalskii) to 20, 28, and 36°C for 2 weeks and then collected their fecal and small-intestinal samples to analyze and compare the microbiota using 16S rRNA gene amplicon sequencing technology. The fecal microbiota displayed more pronounced interspecific differences in microbial community than the small-intestinal microbiota in the two species occurring in thermally different regions. The response of fecal and small-intestinal microbiota to temperature increase or decrease differed between the two species, with more bacterial taxa affected by acclimation temperature in L. reevesii than in P. przewalskii. Both species, the warm-climate species in particular, could cope with temperature change by adjusting the relative abundance of functional categories associated with metabolism and environmental information processing. Functional genes associated with carbohydrate metabolism were enhanced in P. przewalskii, suggesting the contribution of the fecal microbiota to cold-climate adaptation in P. przewalskii. Taken together, our results validate the two hypotheses tested, of which one suggests that the gut microbiota should help lizards adapt to thermal environments in which they live, and the other suggests that microbial communities should be thermally more sensitive in warm-climate lizards than in cold-climate lizards.

Thermal adaptability of neonatal piglets: Early life welfare crisis

Thermal adaptability of neonatal piglets: Early life welfare crisis

Genome-wide sequencing reveals geographical variations in the thermal adaptation of an aquaculture species with frequent seedling introductions

Climate change and human activity are essential factors affecting marine biodiversity and aquaculture, and understanding the impacts of human activities on the genetic structure to increasing high temperatures is crucial for sustainable aquaculture and marine biodiversity conservation. As a commercially important bivalve, the Manila clam Ruditapes philippinarum is widely distributed along the coast of China, and it has been frequently introduced from Fujian Province, China, to other regions for aquaculture. In this study, we collected four populations of Manila clams from different areas to evaluate their thermal tolerance by measuring cardiac performance and genetic variations using whole-genome resequencing. The upper thermal limits of the clams showed high variations within and among populations. Different populations displayed divergent genetic compositions, and the admixed population was partly derived from the Zhangzhou population in Fujian Province, implying a complex genomic landscape under the influence of local genetic sources and human introductions. Multiple single nucleotide polymorphisms (SNPs) were associated with the cardiac functional traits, and some of these SNPs can affect the codon usage and the structural stability of the resulting protein. This study shed light on the importance of establishing long-term ecological and genetic monitoring programs at the local level to enhance resilience to future climate change.

It is hot and cold here: the role of thermotolerance in the ability of spiders to colonize tree plantations in the southern Atlantic Forest.

Worldwide, with the decline of natural habitats, species with reduced niche breadth (specialists) are at greater risk of extinction as they cannot colonise or persist in disturbed habitat types. However, the role of thermal tolerance as a critical trait in understanding changes in species diversity in disturbed habitats, e.g., due to forest replacement by tree plantations, is still understudied. To examine the role of thermal tolerance on the responses of specialist and generalist species to habitat disturbances, we measured and compared local temperature throughout the year and thermotolerance traits [upper (CTmax) and lower (CTmin) thermal limits] of the most abundant species of spiders from different guilds inhabiting pine tree plantations and native Atlantic Forests in South America. Following the thermal adaptation hypothesis, we predicted that generalist species would show a wider thermal tolerance range (i.e., lower CTmin and higher CTmax) than forest specialist species. As expected, generalist species showed significantly higher CTmax and lower CTmin values than specialist species with wider thermal tolerance ranges than forest specialist species. These differences are more marked in orb weavers than in aerial hunter spiders. Our study supports the specialisation disturbance and thermal hypotheses. It highlights that habitat-specialist species are more vulnerable to environmental changes associated with vegetation structure and microclimatic conditions. Moreover, thermal tolerance is a key response trait to explain the Atlantic Forest spider's ability (or inability) to colonise and persist in human-productive land uses.

Fitness surfaces and local thermal adaptation in Drosophila along a latitudinal gradient.

Local adaptation is commonly cited to explain species distribution, but how fitness varies along continuous geographical gradients is not well understood. Here, we combine thermal biology and life-history theory to demonstrate that Drosophila populations along a 2500 km latitudinal cline are adapted to local conditions. We measured how heat tolerance and viability rate across eight populations varied with temperature in the laboratory and then simulated their expected cumulative Darwinian fitness employing high-resolution temperature data from their eight collection sites. Simulations indicate a trade-off between annual survival and cumulative viability, as both mortality and the recruitment of new flies are predicted to increase in warmer regions. Importantly, populations are locally adapted and exhibit the optimal combination of both traits to maximize fitness where they live. In conclusion, our method is able to reconstruct fitness surfaces employing empirical life-history estimates and reconstructs peaks representing locally adapted populations, allowing us to study geographic adaptation in silico.

Harnessing the potential of Pseudoxytenanthera bamboo rhizome for enhanced high-friction assistive solutions

Bamboo cultivation has surged globally, yet environmental concerns arise due to the invasive nature of bamboo rhizomes, often overlooked in favour of shoots. Despite being a renewable resource with rapid growth, rhizomes are underutilized in engineering due to their limited tensile strength from short fibers and hydroxyl group presence. This study explores the untapped potential of Pseudoxytenanthera stocksii (P. stocksii) [Syn. Dendrocalamus stocksii] rhizomes (PSR), enhancing their engineering properties by chemically treating and displacing hydroxyl groups to mitigate their hydrophobic nature. Utilizing the inherent compressive strength of bamboo, treated P. stocksii rhizomes are employed in fabricating composites through hot compression molding, tailored for high-friction assistive applications. Comprehensive analyses, including physical, chemical, and tribological assessments, evaluate PSR for high-friction scenarios, benchmarking them against culm composites and other natural fibers. Results indicate that benzoylated P. stocksii rhizome composites (BPSRC) exhibit properties comparable to, and in some cases surpass, many natural fibre composites. Superior thermal adaptability, revealed through thermogravimetric analysis, positions BPSRC as an eco-friendly alternative to conventional natural fibre composites. Tribological tests underscore the adaptability of PSR composites for high-friction assistive applications, presenting a promising avenue for sustainable bamboo utilization and environmentally conscious engineering solutions.

Optimizing a courtyard microclimate with adaptable shading and evaporative cooling in a hot mediterranean climate

While in recent years significant improvements have been made in the thermal adaptation of indoor building environments, less progress has been made in improving thermal comfort in transitional spaces next to buildings. This makes it imperative to investigate the thermodynamics of such outdoor urban spaces, particularly in warm locations such as southern Spain. The application of suitable passive systems for the regulation of thermal exchanges and the creation of temperate conditions is explored. In order to determine the thermal comfort of users this study focuses on the assessment of two key parameters, mean radiant temperature and relative humidity, and how these are impacted by shading and misting systems in a courtyard. Field measurements analysed the extent to which shading and misting systems enhance user comfort levels in a Seville courtyard. The results indicate a considerable decrease in temperature, with thermal mitigation of up to 11.7 °C below reference points outside the courtyard. The percentage of hours within comfort ranges during heat waves increased by 100% by optimizing the results of an additional experimental campaign and considering results from previous ones. The main novelty of this research lies in the comparative study of standalone and integrated passive cooling strategies aiming to provide optimal results in high temperature and heat wave scenarios. The study findings help understand and estimate the effectiveness of low-cost and easily applicable strategies for maintaining comfort conditions during diurnal and nocturnal cycles in both hot seasons and heat waves.

Information entropy analysis of the relation between climate and thermal adaptation: A case study in hot summer and cold winter region of China

The shift of paradigm from reductionist to holism in the field of thermal adaptation promoted a boom in thermal adaptation theory and provided a systemic perspective on this issue. The thermal adaptation theory confronts twin difficulties of scale and complexity. The complexity and entropy of occupants' thermal adaptation emerging. This paper aims to investigate the information entropy exchange in the coupled climate-building-occupants system. First, we justified that the building-occupants system is an open system and exchanges energy, matter, and entropy with its surrounding climate system. Then, correlation analysis results suggested that climate entropy could influence thermal adaptation entropy. Thermal adaptation entropy fluctuated with climate entropy and the difference varied between −0.5 and 0.5 below 31 °C. A generalized entropy balance model was proposed for quantifying the entropy exchange among coupled climate-building-occupants systems for both free-running and air-conditioning buildings. The quantitative relation between the entropy of thermal adaptation and H_DTR reveals the dynamic occupant's thermal adaptation under the process of entropy exchange with the climate system. The H_DTR and entropy relation models provide a new perspective on thermal adaptation coupled with climate beyond outdoor daily mean temperatures. Control strategies that integrate the dynamic characteristics of DTR can bring more economical, energy-efficient and comfortable effects to the operation of air conditioning or heating systems. Our findings shed some light on the dynamical and complex thermal adaptation. In further studies, more results and methods from meteorological research could be used for thermal adaptation research and interdisciplinary theories could be adopted to help us better understand occupants' thermal adaptation.

Invading new climates at what cost? Ontogenetic differences in the thermal dependence of metabolic rate in an invasive amphibian

Global warming can either promote or constrain the invasive potential of alien species. In ectotherm invaders that exhibit a complex life cycle, success is inherently dependent on the capacity of each developmental stage to cope with environmental change. This is particularly relevant for invasive anurans, which disperse on land while requiring water for reproduction. However, it remains unknown how the different life stages respond in terms of energy expenditure under different climate change scenarios. We here quantified the oxygen uptake of frogs at rest (a proxy of the standard metabolic rate) in the aquatic phase (at the tadpole and climax, i.e. during metamorphosis, stages) and in the terrestrial phase (metamorphosed stage) at three environmental temperatures. To do so, we used marsh frogs (Pelophylax ridibundus), an amphibian with the largest invasive range within the palearctic realm and for which their adaptation to global warming might be key to their invasion success. Beyond an increase of metabolic rate with temperature, our data show variation in thermal adaptation across life stages and a higher metabolic cost during metamorphosis. These results suggest that the cost to shift habitat and face changes in temperature may be a constraint on the invasive potential of species with a complex life cycle which may be particularly vulnerable during metamorphosis.

The community background alters the evolution of thermal performance.

Microbes are key drivers of global biogeochemical cycles, and their functional roles arey dependent on temperature. Large population sizes and rapid turnover rates mean that the predominant response of microbes to environmental warming is likely to be evolutionary, yet our understanding of evolutionary responses to temperature change in microbial systems is rudimentary. Natural microbial communities are diverse assemblages of interacting taxa. However, most studies investigating the evolutionary response of bacteria to temperature change are focused on monocultures. Here, we utilize high-throughput experimental evolution of bacteria in both monoculture and community contexts along a thermal gradient to determine how interspecific interactions influence the thermal adaptation of community members. We found that community-evolved isolates tended toward higher maximum growth rates across the temperature gradient compared to their monoculture-evolved counterparts. We also saw little evidence of systematic evolutionary change in the shapes of bacterial thermal tolerance curves along the thermal gradient. However, the effect of community background and selection temperature on the evolution of thermal tolerance curves was variable and highly taxon-specific,with some taxa exhibiting pronounced changes in thermal tolerance while others were less impacted. We also found that temperature acted as a strong environmental filter, resulting in the local extinction of taxa along the thermal gradient, implying that temperature-driven ecological change was a key factor shaping the community background upon which evolutionary selection can operate. These findings offer novel insight into how community background impacts thermal adaptation.

Polygenic adaptation of a cosmopolitan pest to a novel thermal environment.

The fluctuation in temperature poses a significant challenge for poikilothermic organisms, notably insects, particularly in the context of changing climatic conditions. In insects, temperature adaptation has been driven by polygenes. In addition to genes that directly affect traits (core genes), other genes (peripheral genes) may also play a role in insect temperature adaptation. This study focuses on two peripheral genes, the GRIP and coiled-coil domain containing 2 (GCC2) and karyopherin subunit beta 1 (KPNB1). These genes are differentially expressed at different temperatures in the cosmopolitan pest, Plutella xylostella. GCC2 and KPNB1 in P. xylostella were cloned, and their relative expression patterns were identified. Reduced capacity for thermal adaptation (development, reproduction and response to temperature extremes) in the GCC2-deficient and KPNB1-deficient P. xylostella strains, which were constructed by CRISPR/Cas9 technique. Deletion of the PxGCC2 or PxKPNB1 genes in P. xylostella also had a differential effect on gene expression for many traits including stress resistance, resistance to pesticides, involved in immunity, trehalose metabolism, fatty acid metabolism and so forth. The ability of the moth to adapt to temperature via different pathways is likely to be key to its ability to remain an important pest species under predicted climate change conditions.

Relative thermal pleasure model: A unified framework for understanding and predicting thermal comfort in dynamic environments

Thermal comfort within dynamic environments is shaped by a combination of factors including thermal adaptation levels, short-term thermal histories, thermal expectations, and non-thermal stimuli. However, existing thermal comfort models have fallen short in providing a unified theoretical framework capable of elucidating the combined impact of these factors on thermal comfort. Here, we introduce the Relative Thermal Pleasure (RTP) model, which offers a unified theoretical framework for both elucidating and predicting thermal pleasure. The RTP model posits that the contrast between the target thermal stimulus and its reference points, in conjunction with the distribution of attention weights among these elements, together determine the emergence and intensity of thermal pleasure. To validate the model's predictions and determine model coefficients, nineteen participants were recruited to access their thermal pleasure between two warm environments with different temperatures ranged from 32 to 45 °C. The RTP model clarifies the psychological mechanisms underlying the generation of thermal pleasure and elucidates how external environmental factors and internal psychological and physiological factors influence thermal pleasure. Through theoretical and experimental analysis, we found that improving a poor thermal environment may or may not trigger thermal pleasure, depending on the improvement percentage in the thermal environment. The critical improvement percentage to induce thermal pleasure is 48.7%. The findings of this study have the potential to advance our understanding of thermal comfort and guide us in creating more comfortable thermal environments.

Assessing Ecogeographic Rules in Two Sigmodontine Rodents along an Elevational Gradient in Central Chile.

Bergmann's and Allen's rules are two classic ecogeographic rules concerning the physiological mechanisms employed by endotherm vertebrates for heat conservation in cold environments, which correlate with adaptive morphological changes. Thus, larger body sizes (Bergmann's rule) and shorter appendages and limbs (Allen's rule) are expected in mammals inhabiting cold environments (higher latitudes). Both rules may also apply to elevational gradients, due to the decrease in external temperature as elevation increases. In this study, we evaluated whether these patterns were true in two coexisting sigmodontine rodents across an elevational gradient in central Chile. We analyzed whether the size of the skull, body, and appendages of Abrothrix olivacea (n = 70) and Phyllotis darwini (n = 58) correlated with elevation, as predicted by these rules in a range between 154 and 2560 m. Our data revealed weak support for the Bergmann and Allen predictions. Moreover, we observed opposite patterns when expectations of Bergmann's rules were evaluated, whereas Allen's rule just fitted for ear size in both rodent species. Our results suggest that morphological changes (cranial, body, and appendage sizes) may play a minor role in the thermoregulation of these two species at high elevations, although behavioral strategies could be more critical. Other ecological and environmental variables could explain the morphological trends observed in our study. These hypotheses should be assessed in future studies to consider the relative contribution of morphology, behavior, and physiological mechanisms to the thermal adaptation of these two rodent species at high elevations.

Effect of preferable wind directions on personal thermal comfort of occupants in the air-conditioned office in hot-humid climate

The utilization of a desk fan in the personal thermal comfort (PTC) method is a thermal adaptation that provides indoor comfort and energy-saving for office applications due to the high energy consumption of ACs in the HVAC sector. Researchers have conducted numerous studies on this utilization. However, the question of which wind direction provides thermal comfort for respondents remains an issue, along with the effect of different wind directions on thermal comfort and its influence on skin temperature. Field measurements were conducted inside an air-conditioned office room at a set point temperature of 29.0 °C, involving 400 questionnaire samples from 20 respondents. There were three stages of desk fan configurations; Stage 1 No-fan, Stage 2 Single fan, and Stage 3 Dual fan configurations. The result showed that most respondents preferred wind direction from front and head. Meanwhile, left and right were least favoured. Different wind directions from fans successfully lowered the heat sensation perceived by respondents up to 53% compared to the no-fan configuration. It improved the overall comfort from slightly uncomfortable to moderately comfortable, on a scale of 3.5 to 2.4, under the indoor temperature of 28.6 °C. Respondents' initial average skin temperature during no-fan configuration, ranging from 33.9 to 35.4 °C, was lowered during fan configurations, recorded at 33.2–34.5 °C. PTC setup used in the study also provides lower energy consumption, ranging from 8-20%, compared to low AC set point temperatures of 20.0 and 24.0°C commonly used in hot and humid tropical countries.

Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma.

This study focuses on optimizing the mutagenesis process for Morchella eximia (Mel-7) mycelia through atmospheric and room temperature plasma (ARTP) mutation and explores the resultant thermal adaptability and physiological responses of mutant strains. This research demonstrated a clear relationship between ARTP mutagenesis exposure duration and lethality rate, indicating that an exposure time of 40 s resulted in the optimal balance of inducing mutations without causing excessive mortality. Additionally, this study established 43 °C as the ideal screening temperature for identifying mutant strains with enhanced heat resistance, as this temperature significantly challenges the mycelia while allowing thermotolerant strains to be distinguishable. Among the screened mutants, strains L21, L23, L44, and L47 exhibited superior growth and high-temperature tolerance, with notable resilience at 30 °C, highlighting their enhanced adaptability to above-optimal temperatures. Furthermore, this research delved into biochemical responses, including lipid peroxidation and non-enzymatic antioxidant content, highlighting the diverse mechanisms, such as enhanced lipid peroxidation resistance and increased antioxidant content, employed by mutant strains to adapt to temperature fluctuations. The activities of antioxidant enzymes, including peroxidase (POD) and superoxide dismutase (SOD), were shown to be significantly influenced by temperature elevations, illustrating their critical roles in the thermal adaptation of mutant strains. These findings shed light on the importance of considering mutation duration and temperature screening in the development of thermotolerant fungal strains with potential applications in various industries. This study's breakthrough lies in its comprehensive understanding of the thermal adaptability of Mel-7 mycelia and the identification of promising mutant strains, offering valuable insights for both academic and industrial purposes.

Enhancing electrical output and thermal adaptivity in an interlocked core-sheath triboelectric yarn/fabric for intelligent fire-rescue systems

Self-powered flexible electronic textiles designed for fire-rescue functions, especially in high-temperature environments, have attracted considerable attention. However, their practical applications are hindered by limited electrical output and poor thermal adaptability. In this study, we develop an interlocked core-sheath triboelectric yarn (ICSTY) employing a well-established braiding process, which comprises an inner layer of braided conductive yarn and an outer layer of loop-piled polyimide yarn. This unique structural design resulted in the ICSTY exhibiting an enhanced electrical output performance of 16.3 V per length of 15 cm and excellent thermal adaptability across a wide temperature range of 25–250 °C. The woven ICSTY fabric demonstrates outstanding thermal insulation and biomechanical energy harvesting compared with existing firefighting uniforms. Furthermore, it can be seamlessly integrated into a self-powered fire-rescue system for motion monitoring, location acquisition, and escape warnings for trapped firefighters. This exceptional ICSTY holds the potential to significantly impact the future development of intelligent firefighting uniforms.

Detection of evolutionary conserved and accelerated genomic regions related to adaptation to thermal niches in Anolis lizards.

Understanding the genetic basis for adapting to thermal environments is important due to serious effects of global warming on ectothermic species. Various genes associated with thermal adaptation in lizards have been identified mainly focusing on changes in gene expression or the detection of positively selected genes using coding regions. Only a few comprehensive genome-wide analyses have included noncoding regions. This study aimed to identify evolutionarily conserved and accelerated genomic regions using whole genomes of eight Anolis lizard species that have repeatedly adapted to similar thermal environments in multiple lineages. Evolutionarily conserved genomic regions were extracted as regions with overall sequence conservation (regions with fewer base substitutions) across all lineages compared with the neutral model. Genomic regions that underwent accelerated evolution in the lineage of interest were identified as those with more base substitutions in the target branch than in the entire background branch. Conserved elements across all branches were relatively abundant in "intergenic" genomic regions among noncoding regions. Accelerated regions (ARs) of each lineage contained a significantly greater proportion of noncoding RNA genes than the entire multiple alignment. Common genes containing ARs within 5 kb of their vicinity in lineages with similar thermal habitats were identified. Many genes associated with circadian rhythms and behavior were found in hot-open and cool-shaded habitat lineages. These genes might play a role in contributing to thermal adaptation and assist future studies examining the function of genes involved in thermal adaptation via genome editing.

Speed of thermal adaptation of terrestrial vegetation alters Earth's long-term climate.

Earth's long-term climate is driven by the cycling of carbon between geologic reservoirs and the atmosphere-ocean system. Our understanding of carbon-climate regulation remains incomplete, with large discrepancies remaining between biogeochemical model predictions and the geologic record. Here, we evaluate the importance of the continuous biological climate adaptation of vegetation as a regulation mechanism in the geologic carbon cycle since the establishment of forest ecosystems. Using a model, we show that the vegetation's speed of adaptation to temperature changes through eco-evolutionary processes can strongly influence global rates of organic carbon burial and silicate weathering. Considering a limited thermal adaptation capacity of the vegetation results in a closer balance of reconstructed carbon fluxes into and out of the atmosphere-ocean system, which is a prerequisite to maintain habitable conditions on Earth's surface on a multimillion-year timescale. We conclude that the long-term carbon-climate system is more sensitive to biological dynamics than previously expected, which may help to explain large shifts in Phanerozoic climate.

Abstract 1480 Heat Stress, Thermal Adaptation and the Rise of Fungal Diseases

Abstract 1480 Heat Stress, Thermal Adaptation and the Rise of Fungal Diseases