Working mothers experience a lot of physical and mental stress due to their various roles in the home and in society. However, because of the many demands on their time, they sometimes struggle to manage these health concerns effectively on their own. To fill some of these gaps in healthcare access it is necessary to implement a program that can help manage the health of working mothers within a company. The authors of this study aim to identify quality of life and mental health problems common among working mothers and to determine the effects of an in-house health care program to manage those problems. Fourteen female participants who are working and have children were recruited and measurement of health-related quality of life, degree of depression and anxiety and pain intensity were taken. Participants were then enrolled in an in-house health care program for 8 weeks. The in-house health care program, consisted of physical therapy, psychiatrist consulting, and group education. After the program concluded, all of the dependent values were improved. Therefore, this in-hose health care program has shown to be effective for improving the mental health and quality of life of working mothers.