Theoretical Basis Research Articles

The P-type calcium pump Spf1 regulates immune response by maintenance of the endoplasmic reticulum-plasma membrane contacts during Candida albicans systemic infection

ABSTRACT Spf1 is an important P-type ATPase in Candida albicans, which functions as an endoplasmic reticulum calcium pump to maintain calcium homoeostasis. The deficiency of Spf1 attenuates the virulence of C. albicans. However, its impact on immune response remains to be investigated. This study discovered that deletion of SPF1 resulted in a reduction of endoplasmic reticulum-plasma membrane contacts, an important structure mediating material and information exchange. This effect was attributed to the reduced plasma membrane localisation of the crucial endoplasmic reticulum-plasma membrane tethering proteins Ist2 and Tcb1/3. The reduction of the contacts led to a decrease in secretion of the virulence factors phospholipase, secreted aspartyl protease (SAP), candidalysin, and the cell wall-anchored protein Hwp1 during infection. Immunofluorescence staining and quantitative PCR assays further showed that the SPF1 deletion led to a remarkable decrease in the levels of pro-inflammatory cytokines, suggesting the alleviation of the fungus-induced inflammatory response. Ultimately, the regulatory role of Spf1 in immune response significantly weakened the infectivity of C. albicans, and increased the survival rate of the hosts. This finding elucidated the role of fungal calcium pump-governed endoplasmic reticulum-plasma membrane contacts in regulation of immune response. It also makes it possible to regulate the host’s immune response via control of SPF1 expression and functions, providing a theoretical basis for treating fungal infections.

A Comprehensive Quality Evaluation for Gentiana Rigescens Franch. by Fingerprinting Combined with Chemometrics and Network Pharmacology.

Gentiana rigescens Franch. (G. rigescens) is a unique traditional medicinal herb from southwestern China, and its clinical mechanism for the treatment of hepatitis and the quality differences between different origins are not clear. The research aims to analyze the mechanisms for the treatment of hepatitis and differences in inter-origin differences using analytical techniques, chemometrics, and network pharmacology. Through infrared spectroscopy, spectral images, and high-performance liquid chromatography (HPLC) analysis, it was found that there were differences in absorbance intensity and significant differences in compound content among the samples' origin. G. rigescens iridoids and flavonoids exert therapeutic effects on hepatitis through multiple targets (GAPDH, EGFR, and MMP9, etc.) and multiple pathways (non-small cell lung cancer, hepatitis C, etc.). The above HPLC, chemometrics, and network pharmacology results revealed that gentiopicroside, and swertiamarine was the best quality marker among origins. The accuracy of the ResNet model train, test, and external validation sets for synchronous spectral images were 100 %, which could be utilized as an effective tool for tracing G. rigescens's origins. The R2 of the calibration and validation sets of the PLSR model was higher than 0.70. This model had excellent predictive performance in determining the content of gentiopicroside and swertiamarine, and could quickly, accurately, and effectively predict these two compounds. The research investigates the differences in G. rigescens origins from multiple perspectives, establishes image recognition models and prediction models, and provides new methods and theoretical basis for quality control of G. rigescens.

Turbulence correlation between moving trains and anemometer towers: Theoretical analysis, field measurements and simulation

A precise command of railway operations according to the measured instantaneous wind speed on an anemometer tower along a railway line is the development trend, whose challenges lie in the unknown transfer relation(s) between wind speed fluctuations on a moving train and an anemometer tower, i.e. the turbulence correlation between them. To address this issue, in the current work, the cross-correlation functions of wind speed fluctuations at the moving train and anemometer tower are derived, and an empirical formula of the coherence functions is obtained. The turbulence correlation is inversely related to the separation distance from the anemometer tower to the line, and there is little correlation when this distance is longer than double the longitudinal turbulence length scale. Field measurements of wind characteristics were carried out on an anemometer tower and a moving vehicle, and the turbulence correlation and its expression were validated. Three methods are proposed and compared to evaluate the instantaneous wind speed at the anemometer tower and moving train with this correlation. The methods based on the cross-spectral density and coherence function can accurately simulate the correlation, and the latter performance is slightly better (its simulation of the frequency domain correlation is 52.9% better than the former); the method based on solely independent and identically distributed random phases cannot fully simulate the correlation. From this, the effects of the correlation on train operations are studied and analysed in detail. Our analysis shows that neglecting this correlation leads to conservative estimates: wind speed differences between the anemometer tower and the moving train are at least 18.1% greater, and the safety and economic assessments of train operations in crosswinds are underestimated by at least 32.0%. Considering the correlation can reduce the (excess) safety risk/margin and is an inevitable development of adapting to the detailed assessment of the crosswind stability of vehicles. The quantitative description and simulation of the correlation presented in this work point to the critical importance of wind speed monitoring systems for the detailed crosswind assessment, and provide a theoretical basis for further research work on the crosswind stability of vehicles under true/realistic turbulent flow wind.

Tweeting for Influence: Analysing France and China’s Cultural Diplomacy on Social Media, a Mixed Methods Approach

Abstract How are states using cultural diplomacy on social media to expand their power? This research analyses over 67,000 tweets from the French and Chinese Ministries of Foreign Affairs (mfa s) as well as their respective state-sponsored cultural networks including Institut Français, Alliance Française, and the Confucius Institutes. Stephen Lukes’ three-dimensional power framework, where cultural authority legitimizes power over others, provides the study’s theoretical basis. In analysing the tweets, I employ a mixed methods approach including vader sentiment analysis, word frequency analysis, and thematic analysis. While both states aim to present their cultures as high-value cultures, the similarities end there. As a liberal democracy, France encourages open expression through its cultural institutions, including discourse critical of France. In contrast, China’s cultural diplomatic messaging is precisely controlled and speaks about China in invariably exalting terms. This study offers evidence that regime type has a powerful effect on cultural diplomatic messaging on social media.

Nanoparticles and their application in the diagnosis of hepatocellular carcinoma

Abstract Most patients are at advanced stages when they are diagnosed with hepatocellular carcinoma, leading to poor prognosis and a low 5-year survival rate. Serological markers, ultrasound, computed tomography, magnetic resonance imaging, positron emission tomography, and liver biopsy are the common clinical diagnostic techniques for liver cancer. Effective interventions in the early stage will be beneficial to improve the prognosis of liver cancer patients and reduce the global burden. Therefore, it is urgent to develop new diagnostic methods to improve the diagnosis and management of liver cancer. Nanotechnology has become a new frontier subject in medical detection along with the application of nanomaterials in the manufacture of drug carriers, diagnostic tools, and therapeutic devices. Many studies have shown that nanoparticles (NPs) can be applied to the clinical diagnosis of liver cancer in combination with existing technologies, providing a new method for the early diagnosis of liver cancer. In this review, we elaborate on the theoretical basis and characteristics of NPs in the diagnosis of liver cancer, and the research progress and prospects of NPs in the diagnosis of liver cancer are summarized.

Analysis and optimization of the efficient press shop operation

Abstract. In the last decade, stamping and forming manufacturing methods have been widely used in the manufacturing industry, and the stamping and forming industry has gradually shifted from the competition of scale and quantity to the competition of high quality, cost-effectiveness, and multi-category. Therefore, it is of far-reaching significance to study the efficient operation method of the press shop. In this paper, the efficient operation modeling method, digital simulation platform, and production line improvement of the press shop are reviewed. According to analysis, it can be concluded that the dynamic data-driven modeling method is perfect, but the theoretical basis involved in the practical application process is too complicated, therefore, press shop managers may be required to have a certain degree of theoretical basis so that the method can be effectively realized. Besides, the virtual-real combination of digital simulation platforms is easy to operate in the press shop, but the model assumptions analyzed in this paper are too idealized, and potential unresolved problems may arise in the practical application of the press shop with a larger scope and wider coverage. Finally, the production-based transformation of the shop floor production line is a more suitable management method for older factories that are ready for upgrading.

Excelling Green Supply Chain Management Practices Through Maturity Model: A Case Study of Fish 96 Company

This research aims to improve GSCM practices at Fish 96, an SME in tilapia aquaculture located in Bandung, West Java, Indonesia. It identifies and addresses some critical business issues regarding reducing the DOA rate, improving operational efficiency, and reducing environmental impacts. The literature review forms the theoretical basis of GSCM practices such as green procurement, manufacturing, distribution, and reverse logistics. A new GSCM maturity model is developed and validated with the case study of Fish 96, which eval_uates the company's current maturity levels and structured improvements. It assesses that Fish 96 stands at the initial stage in the journey of GSCM. Most practices are either nonexistent or at an initial point of implementation. The suggested maturity model includes five levels: Nonexistent, Aware, Intermediate, Advanced, and Sustainable, which represent a clear roadmap for Fish 96 to improve its GSCM performance. The research recommends adopting broad green practices across all supply chain dimensions, emphasising systematic strategies and enhanced supplier engagement by focusing on efficiency measures. With these recommendations, Fish 96 will contribute to reducing operational costs while reducing environmental impacts and improving social outcomes, thus improving the company's long-term sustainability and competitiveness.

ФОРМУВАННЯ КЛЮЧОВОЇ КОМПЕТЕНТНОСТІ «ПІДПРИЄМЛИВІСТЬ ТА ФІНАНСОВА ГРАМОТНІСТЬ» В ОСВІТНЬОМУ ПРОЦЕСІ З ГЕОГРАФІЇ

The article is devoted to the problem of forming the key competence “entrepreneurship and financial literacy” by means of the school geography course in basic school. The authors analyze the sequence of implementation of this key competence in the educational process and the transformation of the category wording in domestic legislative and regulatory documents. The importance of the theoretical discourse on the content of the concept of “entrepreneurial competence” and the consideration of studies of the phenomenon in the social, psychological, pedagogical and content aspects is emphasized. The authors substantiate that in order to form entrepreneurial competence and financial literacy, it is important to clarify the peculiarities of the structure of this competence and determine the characteristics of the structural components. Based on the achievements of domestic and foreign scholars, the author's vision of the structure of entrepreneurship and financial literacy competence is proposed; the motivational, personal, cognitive and activity components are distinguished. In view of the problem under study, the characteristics of each structural component in the context of the content of the school geography course are proposed. The article analyzes the potential of the content of geography curricula/model programs in basic school as a theoretical basis for the formation of the key competence “entrepreneurship and financial literacy”. Particular attention is paid to the courses of economic and social geography in the 9th grade. Emphasis is placed on the importance of ensuring the integration of geography and economics content in the educational process to promote understanding of economic processes, forming students' readiness for entrepreneurship, developing critical thinking, responsibility and initiative. The authors draw attention to the changes in the methodology of competency-based teaching of geography and the importance of introducing methods that will help motivate students to succeed, willingness to act at risk, the ability to solve actual problems using practical tasks and situations, the ability to build interpersonal relationships, and responsibility for the implementation of a common cause.

The Knob Domain of the Fiber-1 Protein Affects the Replication of Fowl Adenovirus Serotype 4

Fowl adenovirus serotype 4 (FAdV-4) outbreaks have caused significant economic losses in the Chinese poultry industry since 2015. The relationships among viral structural proteins in infected hosts are relatively unknown. To explore the role of different parts of the fiber-1 protein in FAdV-4-infected hosts, we truncated fiber-1 into fiber-1-Δ1 (73–205 aa) and fiber-1-Δ2 (211–412 aa), constructed pEF1α-HA-fiber-1-Δ1 and pEF1α-HA-fiber-1-Δ2 and then transfected them into leghorn male hepatocyte (LMH) cells. After FAdV-4 infection, the roles of fiber-1-Δ1 and fiber-1-Δ2 in the replication of FAdV-4 were investigated, and transcriptome sequencing was performed. The results showed that the fiber-1-Δ1 and fiber-1-Δ2 proteins were the shaft and knob domains, respectively, of fiber-1, with molecular weights of 21.4 kDa and 29.6 kDa, respectively. The fiber-1-Δ1 and fiber-1-Δ2 proteins were mainly localized in the cytoplasm of LMH cells. Fiber-1-Δ2 has a greater ability to inhibit FAdV-4 replication than fiber-1-Δ1, and 933 differentially expressed genes (DEGs) were detected between the fiber-1-Δ1 and fiber-1-Δ2 groups. Functional analysis revealed these DEGs in a variety of biological functions and pathways, such as the phosphoinositide 3-kinase–protein kinase b (PI3K–Akt) signaling pathway, the mitogen-activated protein kinase (MAPK) signaling pathway, cytokine–cytokine receptor interactions, Toll-like receptors (TLRs), the Janus tyrosine kinase–signal transducer and activator of transcription (Jak–STAT) signaling pathway, the nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) signaling pathway, and other innate immune pathways. The mRNA expression levels of type I interferons (IFN-α and INF-β) and proinflammatory cytokines (IL-1β, IL-6 and IL-8) were significantly increased in cells overexpressing the fiber-1-Δ2 protein. These results demonstrate the role of the knob domain of the fiber-1 (fiber-1-Δ2) protein in FAdV-4 infection and provide a theoretical basis for analyzing the function of the fiber-1 protein of FAdV-4.

Research progress on melatonin, 5-HT, and orexin in sleep disorders of children with autism spectrum disorder.

Sleep disorders are among the common comorbidities of autism spectrum disorder (ASD), which not only affect the daily life and learning ability of children but may also exacerbate other symptoms of ASD, seriously impacting the quality of life of children and their families. Given this, understanding the neurobiological mechanisms of sleep disorders in children with ASD has significant research value for developing effective intervention strategies. Melatonin, 5-HT, and orexin are key neurotransmitters that regulate the sleep-wake cycle. Through in-depth analysis of the biological functions and regulatory pathways of these neurotransmitters, new perspectives may be provided for personalized treatment of sleep disorders in children with ASD. This article reviews the research progress on melatonin, 5-HT, and orexin in sleep disorders among children with autism spectrum disorder, focusing on exploring the mechanisms of these key neurotransmitters in sleep disorders of children with ASD and how they affect the sleep-wake cycle, providing a theoretical basis for improving the sleep quality of children with ASD.

“POLITICALLY MATURE AND EXPERIENCED ONE...”: SOME MODELS OF ROTATION OF THE FIRST SECRETARIES OF THE COMMUNIST PARTY REGIONAL COMMITTEE IN THE 2ND HALF OF THE 1940S TO THE 50S (based on the materials of the Upper Volga regions)

The article analyses the models of rotation of regional leadership in the Upper Volga regions at the end of Stalin’s period and in the beginning of Khrushchev’s one. The focus is on the 3 first secretaries of the Communist Party regional committee in the 1940–50s - Pavel Alfyorov, Vladimir Luk’yanov, Fyodor Titov, who at different times were Party leaders of Vladimir, Ivanovo, and Yaroslavl regions. Attention is paid to quantitative and institutional characteristics associated with personnel rotations of late Stalinism and Khrushchev Thaw. The methodological basis of the study was the principles of historicism and systematicity, the prosopographic method, a combination of analysis of archival documents and sources of personal origin. The theoretical basis of the article is based on the ideas of Yelena Zubkova, Aleksandr Konovalov, Aleksey Fyodorov, Oleg Khlevniuk, Yoram Gorlizki. The source base is represented by documents from the Russian State Archive of Contemporary History, materials from documentary publications and reference publications, and personal sources (memoirs, interviews). The article concludes, that the biographies of the first secretaries of the regional committees of the Upper Volga regions (Pavel Alfyorov, Vladimir Luk’yanov, Fyodor Titov) demonstrate 3 different models of rotation of regional leadership personnel of Stalin and Khrushchev periods, but do not exhaust the diversity of personnel movements in the post-war USSR. It is noted that the first rotation model “From region to region”, inherent in Vladimir Luk’yanov, can be considered typical of the group of successful Stalinist promoters of the late 1930s. It is emphasised that the second model “Region-centre-region”, inherent in Pavel Alfyorov, reflects a move across the regions with a short-term return to the central apparatus of an experienced but not quite successful Party functionary. It is stated that the third model “Second-First-Second”, inherent in Fyodor Titov, was more rare, characterised by alternating the position of first secretary of the regional committee in the regions of the Russian Soviet Republic and second secretary in the republican communist parties of the USSR. The significance for career growth in the 1940-50s is noted considering the positions of second secretary of the regional committee, chairman of the regional executive committee, inspector of the Central Committee of the All-Union Communist Party of Bolsheviks, structures of the central apparatus. As the finishing point in the nomenclature career of Party functionaries of the 1950–60s, the Party Control Committee is indicated. The publication is an invitation to discussion for representatives of the scientific community regarding the typification and classification of regional Party leaders of the Soviet era.

Study on chloride ion penetration performance of recycled composite micro powder concrete under freeze-thaw environment

With the increasing generation of construction waste, the rational utilization of recycled micro powder has become crucial for the efficient development of construction waste resource recycling. This study investigates the chloride ion penetration performance of Recycled Composite Micro Powder Concrete (RCMPC) under freeze-thaw conditions. By incorporating Recycled Concrete Powder (RCP) and Recycled Brick Powder (RBP) in a ratio of 2:8 to replace various proportions of cement, specimens with different water-to-binder ratios (0.35, 0.45, 0.55) were designed and subjected to freeze-thaw cycles (FTC), pore structure analysis, rapid chloride ion migration, and diffusion tests. The results indicate that the frost resistance of the concrete decreases with the addition of Recycled Composite Micro Powder (RCMP). As the RCMP content increases, the mass loss rate and relative dynamic modulus decline, while the thickness of the damage layer increases. Moreover, the chloride ion migration coefficient and diffusion coefficient exhibit a linear increase with the number of FTC, with specimens containing higher RCMP content showing poorer chloride ion penetration performance. Based on the experimental results, this paper establishes a chloride ion diffusion model for RCMPC considering freeze-thaw damage, which demonstrates good predictive capability for the chloride ion diffusion behavior of RCMPC in freeze-thaw environments, providing a theoretical basis for its application in cold and marine regions.

Nephroprotective effect of SGLT2 inhibitors in elderly patients with type 2 diabetes mellitus and hypertension: a real-world population-based cohort study

ABSTRACT Objectives This study aimed to investigate the nephroprotective effect of sodium-glucose cotransporter 2 inhibitors (SGLT2i) in elderly patients with type 2 diabetes mellitus (T2DM) and hypertension based on real-world clinical data. The study aimed to provide a theoretical basis for evidence-based pharmacological treatment of chronic kidney disease in this population. Methods The ‘Health Cloud’ platform of the Shanghai Municipal Health Commission was employed to identify and screen elderly patients with T2DM and hypertension. The propensity score matching cohort was further constructed to estimate the effect of SGLT2i on the risk of rapid decline in renal function (∆eGFR≤-5 ml/min/1.73 m2 or ∆eGFR%≤-5%). Multiple sensitivity analyses were conducted to assess the robustness of the results. Results After propensity score matching, no significant differences of covariates were identified between the SGLT2i and non-SGLT2i groups. The results of multivariate logistic models demonstrated a consistent and inverse correlation between SGLT2i use and the risk of rapid eGFR decline, whether defined as ∆eGFR≤-5 ml/min/1.73 m2 (OR = 0.60, 95% CI:0.38-0.96) or ∆eGFR%≤-5%（OR = 0.57, 95% CI:0.37-0.89). In the stratification of renin-angiotensin system inhibitor (RASi) treatment, SGLT2i was associated with a lower risk of rapid eGFR decline in the RASi group (all ORs < 1, p < 0.05), with no interaction between SGLT2i and RASi (all P for interaction > 0.05) detected. Conclusions SGLT2i significantly reduced the risk of rapid eGFR decline in elderly patients with T2DM and hypertension, but the synergistic effect with RASi remains unclear.

Analysis of Gain Enhancement Techniques in Integrated Circuit Design

Abstract. Today, the size of semiconductor devices is still decreasing, which makes the circuit more integrated, faster computing speed, and lower power consumption. But for a single transistor, some performance degrades. One of the important properties is voltage gain, which must be compensated with some special methods in the design. This paper will focus on the discussion and analysis of four methods, which are able to improve the voltage gain. These are cascode amplifier, cascade amplifier, gain boosting, and bootstrapping. In this letter, their respective advantages and disadvantages will be discussed. Furthermore current applications and possible external development of these methods are also important part of this paper. In this part, the discussion will be further expanded and summarized on the basis of previous studies. Hopefully, these studies can help others gain a more comprehensive understanding of these technologies and provide a theoretical basis for future high-performance analog integrated operational amplifier research and design.

Mechanistic Investigation of the Pyrolysis Temperature of Reed Wood Vinegar for Maximising the Antibacterial Activity of Escherichia coli and Its Inhibitory Activity

To solve the problem of large-scale growth of wetland reeds, wood vinegar, a by-product of pyrolysed reed wood vinegar, can be used as a natural antimicrobial agent. In this study, we compare the changes in growth and bacterial morphology of Escherichia coli (E. coli) treated with reed wood vinegar at different pyrolysis temperatures (300 °C, 500 °C and 700 °C) and reveal the bacterial inhibition mechanism of reed wood vinegar by RNA-Seq. The results of bacteria inhibitory activity showed that 1/2MIC 500 °C wood vinegar had the most prominent bacteria inhibitory activity. qPCR results showed that reed wood vinegar was able to significantly inhibit the expression of E. coli biofilm and genes related to the cell membrane transporter proteins. Electron microscopy observed that the wood vinegar disrupted the cellular morphology of E. coli, resulting in the crumpling of E. coli cell membranes. RNA-Seq showed the multifaceted antimicrobial effects of wood vinegar and demonstrated that the inhibitory effect of wood vinegar on E. coli was mainly realized through the inhibition of the expression of malE, which is an ATP-binding cassette (ABC) transporter complex of E. coli. In conclusion, our study provides an effective method and a theoretical basis for the mechanism of reed wood vinegar as a natural antimicrobial agent and its pathway of bacterial inhibition.

Semantics and Syntax of Old English Motion Verbs

This is a study in the syntax and semantics of Old English verbs of motion, including verbs of neutral motion, verbs of manner of motion and verbs of path of motion. Its aim is to identify the morphosyntactic alternations in which these verbs participate. The theoretical basis of the research draws on Goddard’s semantics of motion, Levin’s model of verb classes and alternations and Talmy’s typological distinction between satellite-framed languages and verb-framed languages. The grammatical patterns of the verbs under analysis are discussed, as described in The Dictionary of Old English (Healey), with special emphasis on the study of transitivity, case, prepositional government, and reflexivity. As far as meaning components are concerned, this work concentrates on the study of polysemy. The conclusions of this study refer to the consistency of the syntactic behaviour of the verbs under analysis and the main alternations found within the verbal classes.

Empirical study of Neo-Chinese fashion consumer behaviour from the perspectives of cultural identity and emotional self-regulation mechanism

PurposeThe purpose of this paper is to explore the influential mechanism of consumer behaviour and identity towards Neo-Chinese fashion from the perspectives of cultural identity and emotional self-regulation mechanism.Design/methodology/approachThrough empirical methods such as questionnaire surveys and regression analysis, nine hypotheses were proposed and tested based on 253 valid samples.FindingsThe results indicate that perceived value has a significant positive impact on pride emotion, anxiety emotion and cultural identity, respectively. Cultural pride (anxiety) emotion also has a significant positive (negative) impact on identity. The study also finds that anxiety emotion plays a significant mediating effect in the relationship between perceived value and cultural identity, which of country-of-origin plays a significant negative moderating role.Originality/valueThis paper reveals the psychological mechanisms and implementation paths through which fashion consumption and Neo-Chinese design drive cultural identity. It provides a theoretical basis for optimizing and innovating strategies to guide the evolution of Neo-Chinese cultural identity in the new context.

Numerical and experimental study of dynamic gas–liquid separator with various viscosities

The gas–liquid separation process is important in various industries, such as electric power, aerospace, and petroleum. This study introduces an innovative, dynamic gas–liquid separator (DGLS) in which a cyclonic flow pattern is induced by blade rotation. This cyclonic flow enhances the efficiency of gas and liquid phase separation while also imparting energy to facilitate the transport of the separated fluid. Numerical simulations are used to analyze the internal flow dynamics, power requirements, and separation efficiency of this DGLS. A comparison with experimental results is conducted to validate the reliability of the numerical model. The effects of liquid-phase viscosity on the internal energy consumption and separation performance of the DGLS are explored at various flow rates. The simulation results indicate that for a given viscosity, the degassing rate of the separator decreases while the liquid removal rate increases as the inlet flow rate rises. Furthermore, it is observed that higher viscosity leads to poorer separation performance, with a decrease in turbulent kinetic energy near the rotating axis and an increase in turbulence intensity near the wall. At lower flow rates, the effectiveness of liquid-phase outlet pressurization improves with increasing viscosity. However, at higher flow rates, increasing viscosity leads to a substantial decline in energy performance and a reduction in liquid-phase outlet pressurization. The increment in turbulent kinetic energy is greater than the square of the mean velocity, indicating a positive correlation between turbulence intensity and turbulent kinetic energy. These findings not only provide a theoretical basis for the prediction of flow losses within a DGLS and the efficient design of these separators, but also provide guidance for industrial applications involving high-viscosity fluids.

Analytical study on plastic loosening zone of weak surrounding rocks tunnel in high cold areas

AbstractThe analysis of plastic loosening zone of tunnels with cold condition, high in situ stress, and weak stratum is the key scientific problem of tunnel construction in cold areas. Based on the unified strength theory and considering the coupling effect of high in situ stress and low‐temperature field, the analytical model of the plastic loosening zone of tunnel surrounding rocks was proposed, and the influence laws of physical parameters (initial ground stress, freezing temperature, excavation radius, and intermediate principal stress) was explored. The results illustrate that: (1) with the coupling of low temperature and stress fields, the in‐situ stress has a more significant effect on the radius of the plastic loosening zone of the tunnel surrounding rocks, showing a nonlinear correlation. When the in situ stress is small, the influence of the temperature field on the radius of the plastic loosening zone is more obvious. (2) The radius of the plastic loosening zone of the tunnel surrounding rocks increases synchronously with the excavation radius and gradually decreases with the increase of the intermediate principal stress. (3) The greater the temperature difference in the low‐temperature field, the tunnel stability has a more significant response to the intermediate principal stress and is positively correlated with the coefficient of the intermediate principal stress. (4) In the actual project, the proposed model can well describe the mechanical behavior and deformation characteristics of tunnel surrounding rocks in low‐temperature environment, showing applicability. The research results are expected to provide a theoretical basis for the study of tunnel stability in cold areas.

Vertical fungal community distribution patterns along a stratified soil profile in subalpine Larix principis-rupprechtii plantations on China's Luya mountain

Soil microorganisms play important roles in the biogeochemical cycling of terrestrial ecosystems. Recent studies found that soil fungal composition and diversity varied significantly with soil depth. However, little is known about the vertical distribution patterns of soil fungal communities and their associated drivers. For this study, we collected soil samples from six soil layers (i.e., litter layer (P), humus layer (P0), 0–10 cm layer (P1), 10–20 cm layer (P2), 20–40 cm layer (P3), and 40–80 cm layer (P4)) within Larix principis-rupprechtii plantations to investigate fungal community composition, diversity, and associated drivers using Illumina MiSeq high-throughput sequencing. Results showed that the top 10 dominant genera with the highest relative abundance belonged to Ascomycota, Basidiomycota, and Mortierellomycota. A decreasing α-diversity trend was observed along with soil depth. Moreover, correlation analysis showed that ammonia nitrogen (NH4+-N), pH, total carbon (TC), and total nitrogen (TN) content markedly correlated with fungal α-diversity. Significant β-diversity differences were found in soil fungal communities. Additionally, TN and total phosphorus (TP) content were the main environmental drivers that influenced the spatial distribution pattern of fungal communities. The βNTI showed a gradual increase with soil depth. In the surface layers of soil, the dominant factor of fungal community assembly was homogeneous selection, while in the deep layers of soil, it was variable selection. Co-occurrence network analysis showed that fungal community interactions in the deepest soil layer (40–80 cm) were more complex and were more positive. Results from this study provide a theoretical basis and data support to understand the mechanisms of soil fungal community assembly processes more deeply in L. principis-rupprechtii plantations.