Theoretical Basis Research Articles

Barriers and facilitators to implementing imaging-based diagnostic artificial intelligence-assisted decision-making software in hospitals in China: a qualitative study using the updated Consolidated Framework for Implementation Research

ObjectivesTo identify the barriers and facilitators to the successful implementation of imaging-based diagnostic artificial intelligence (AI)-assisted decision-making software in China, using the updated Consolidated Framework for Implementation Research (CFIR) as...

A Quantitative Method of Assessing the Hazard Perception of Drivers in Human–Machine Codriving Vehicles

Hazard perception, as a dynamic indicator, intuitively characterizes drivers’ ability to detect potential hazard events in complex driving environments. However, current quantitative research on the hazard perceptions of drivers in the human–machine codriving phase is lacking. A total of 30 drivers were recruited to carry out a driving simulator experiment, and a total of 8 risky driving scenarios were experienced. The physiological characteristics and eye movement characteristics of the drivers were collected. An expert evaluation method was selected to quantify the situational hazard degree (SHD). The questionnaire was used to collect the drivers’ subjective hazard evaluations (SHEs) of the scenarios. Paired samples t test and correlation analysis were adopted to screen the evaluation indexes of the drivers’ hazard perceptions. A gray near-optimal comprehensive evaluation model was established to quantify the human–machine codrivers’ hazard perceptions in various risky driving scenarios. The results show that the drivers’ SHEs increased with the SHD. The standard deviation of heart rate, mean pupil diameter, and mean fixation duration can be used as evaluation indexes for the human–machine codrivers’ hazard perceptions. The proposed quantitative method is expected to shift the development of human–machine codrivers’ hazard perception from qualitative analysis to quantitative calculation. The research results can provide a theoretical basis for increasing safety in the human–machine codriving phase, improving road safety and reducing the incidence of accidents.

Propagation characteristics of show sea seismic wave in different underwater media

The propagation characteristics of sea seismic wave (SSSW) in different underwater media are different. This study mainly focuses on the propagation characteristics of SSSW in different underwater media to improve the detection ability of SSSW mines. The propagation of SSSW in different underwater media, including clay, sandy clay, sandstone, mudstone, hard stone, and dolomite, is simulated using the staggered grid finite difference method. The dispersive energy maps of different underwater media are extracted using the f-k dispersive energy extraction method. Compared with the results of Scholte wave dispersion characteristic equations, the simulation results are consistent with the theoretical results. At the same time, the attenuation law of the SSSW train is obtained. The results provide a theoretical basis for developing SSSW mine fuses.

Phylogeography and Population Variation in Prunus discoidea (Prunus subg. Cerasus) in China.

Prunus discoidea is a unique cherry blossom germplasm resource native to China. It is widely distributed across the provinces of Anhui, Zhejiang, Jiangxi, Jiangsu, and Henan, with significant variation. We employed phylogeographic analysis to reveal the evolutionary history of P. discoidea to better understand its genetic diversity and structure. This study provides more accurate molecular insights for the effective conservation and utilization of this germplasm resource. We conducted a phylogeographic analysis of 348 individual plants from 13 natural populations using three fragments (rpoB, rps16, and trnD-E) of chloroplast DNA (cpDNA) and one fragment (ITS) of ribosomal DNA. The results revealed that P. discoidea demonstrates a significant level of genetic diversity (Hd = 0.782; Rd = 0.478). Gene flow among populations was limited, and the variation within populations was the main source of genetic diversity in P. discoidea (among populations: 34.26%, within populations: 65.74%). Regarding genetic differences among populations, Nst (0.401) showed greater differences than Gst (0.308; p < 0.05), demonstrating that there was a significant geographical structure of lineage. One lineage was the central region of Anhui and the western region of Hubei. The other lineage was the Jiangsu region and the Zhejiang region. P. discoidea diverged from Prunus campanulata approximately 1.5 million years ago, during the Pleistocene epoch. This study provides a scientific theoretical basis for the conservation and utilization of germplasm resources of P. discoidea.

ON THE QUESTION ABOUT THE FEATURES OF THE CONDITION OF THE FETUS IN PREGNANT WOMEN WITH THYROID DISEASES

The purpose of the study is to assess the functional state of the fetus in pregnant women with certain thyroid diseases. Material and methods.The results of instrumental studies in 118 pregnant women, who were divided into three clinical groups, were analyzed. Group 1 (44 people) consisted of patients with autoimmune thyroiditis (AIT). Group 2 (52 people) consisted of patients with diffuse toxic goiter (DTG). The 3rd group, which was control in relation to the other two, consisted of 22 pregnant women who had no somatic pathology and gestational complications. The diagnosis of thyroid diseases was made by an endocrinologist during a consultation in the third trimester of pregnancy on the basis of laboratory and instrumental data. The functional state of the fetus was assessed based on the total result of cardiotocographic and ultrasound studies, for which Partecust (Siemens, Germany) and Aloka-1700 (Hitachi, Japan) devices were used. Statistical processing of clinical material was carried out using StatSoft software, Russia. Research results. A comprehensive assessment of the adaptive capabilities of the fetus showed changes in its functional state in pregnant women with thyroid pathology. Prognostically, the most important thing in pregnant women with autoimmune thyroiditis and diffuse toxic goiter is a decrease to 0-1 points in indicators that determine motor activity and tone, respiratory movements, as well as the reaction to a non-stress test. Among patients with autoimmune thyroiditis and diffuse toxic goiter, a high assessment of the biophysical profile of the fetus occurred 4 and 3.4 times, respectively, less often than among women with a normal pregnancy (among pregnant women without somatic pathology and gestational complications). Conclusion. Disturbances in the function of the thyroid gland during the gestational period can have an adverse effect on the development of the fetus, which is a theoretical basis for the development of treatment and preventive methods aimed at improving perinatal outcomes in this group of patients

Study on Soil Stabilization and Slope Protection Effects of Different Plants on Fully Weathered Granite Backfill Slopes

The slope erosion in the distribution area of completely weathered granite is often relatively severe, causing serious ecological damage and property loss. Ecological restoration is the most effective means of soil erosion control. Taking completely weathered granite backfill soil as the research object, two types of slope protection plants, Vetiver grass and Pennisetum hydridum, were selected. We analyzed these two herbaceous plants’ soil reinforcement and slope protection effects through artificial planting experiments, indoor simulated rainfall experiments, and direct shear tests. The test results showed that the runoff and sediment production rates of the two herbaceous plant slopes were significantly lower than those of the bare slope, with the order of bare slope &gt; Vetiver grass slope &gt; Pennisetum hydridum slope. Compared with the bare slope, the cumulative sediment production of the Vetiver grass slope at 60 min decreased by 56.73–60.09%, and the Pennisetum hydridum slope decreased by 75.97–78.45%. The indoor direct shear test results showed that soil cohesion decreases with increasing water content. As the root content of Vetiver grass roots increases, soil cohesion first increases and then decreases, reaching a maximum value when the root content is 1.44%. As the root content of Pennisetum hydridum increases, soil cohesion increases. The internal friction angle increases slightly with increasing water content, while the root content does not significantly affect the internal friction angle. Therefore, the shear strength of soil decreases when the water content increases. The shear strength of the Vetiver grass root-soil composite reaches a peak at a root content of 1.44%, while the shear strength of the giant king grass root-soil composite increases as the root content increases. At the same root content, the shear strength of the Vetiver grass root-soil composite is slightly higher than that of giant king grass. The reinforcement effect of roots on shallow soil is better than on deep soil. Both herbaceous plants have an excellent soil-fixing and slope-protecting impact on the fully weathered granite backfill slope. Pennisetum hydridum’s soil and water conservation effect is significantly better than that of the Vetiver grass. In contrast, Vetiver grass roots slightly outperform Pennisetum hydridum in enhancing the shear strength of the soil. The research results can provide a theoretical basis for the vegetation slope protection treatment of fully weathered granite backfill slopes.

Treating the Symptoms as Well as the Root Causes: How the Digital Economy Can Mitigate the Negative Impacts of Land Resource Mismatches on Urban Ecological Resilience

In the era of the digital economy (DE), the traditional economic growth paradigm is no longer applicable. To explore whether the DE can improve the urban ecological problems left behind by rough economic growth, this study examines the effects of land resource mismatch (LRM) on urban ecological resilience (UER) and evaluates the mitigating influence of the DE. This analysis utilizes data from 280 prefectural-level cities in China over the period from 2007 to 2021 and reveals that LRM significantly undermines UER, with this conclusion remaining robust across a series of tests. Additionally, the detrimental impact of LRM on UER is more pronounced in megacities, cities with high levels of economic development, and those with a lower degree of advanced industrial structure. In further analysis, this study finds that the digital economy can optimize the allocation of land resources, thereby enhancing urban ecological resilience, which has the effect of “treating the root causes”. In addition, digital government and digital infrastructure, as key elements of the digital economy, also mitigate the negative impacts of land resource misallocation on urban ecological resilience, having the effect of “treating the symptoms”. Finally, this study proposes policy suggestions such as optimizing ecological layout, deepening land reform, and promoting digital government and infrastructure construction to provide a theoretical basis and practical guidance for local governments to enhance UER and help build a new model of greener, more resilient, and sustainable urban development.

Comparative Analysis of the Mitochondrial Genome of Eggplant (Solanum melongena L.) to Identify Cytoplasmic Male Sterility Candidate Genes.

Cytoplasmic male sterility (CMS) is important for commercial hybrid seed production. However, it is still not used in eggplant (Solanum melongena L.), and corresponding regulatory genes and mechanisms of action have not been reported. We report CMS line 327A, which was derived from the hybridization between cultivated and wild eggplants. By looking at different stages of anther development under a microscope, we saw that the 327A anther's tapetum layer vacuolized during meiosis, which caused abortion. To investigate the 327A CMS regulatory genes, the mitochondrial genomes of 327A and its maintainer line 327B were assembled de novo. It was found that 15 unique ORFs (Open Reading Frame) were identified in 327A. RT-PCR and RT-QPCAR tests confirmed that orf312a and orf172a, 327A-specific ORFs with a transmembrane domain, were strongly expressed in sterile anthers of 327A. In addition, orf312a has a chimeric structure with the ribosomal protein subunit rpl16. Therefore, orf312a and orf172a can be considered strong candidate genes for CMS. Concurrently, we analyzed the characteristics of CMS to develop a functional molecular marker, CMS312, targeting a future theoretical basis for eggplant CMS three-line molecular breeding.

Winter forage crops influence soil properties through establishing different arbuscular mycorrhizal fungi communities in paddy field

Winter planting is promising for improving the utilization rate of fallow paddy fields in southern China by establishing arbuscular mycorrhizal fungi (AMF) communities. However, the effects of different winter forage crops on AMF community construction remain unknown. The AMF community establishment of different winter planting forage crops were conducted in oat, rye, Chinese milk vetch, and ryegrass, with winter fallow as a control. The AMF colonization rate, soil AMF spore density, community structure and diversity, and soil physicochemical properties were determined. The results showed that the total nitrogen and available nitrogen in winter Chinese milk vetch were 11.11% and 16.92% higher than those in winter fallow (P < 0.05). After planting winter forage crops, the AMF spore density in winter oat, rye, Chinese milk vetch, and ryegrass soil were 127.90%, 64.37%, 59.91%, and 73.62% higher than that before planting, respectively (P < 0.05). Claroideoglomus was the dominant AMF genus in the soil of winter planting oat, rye, and ryegrass. The average membership function value of winter Chinese milk vetch was the highest, indicating that it had the best comprehensive effect on soil physicochemical properties, AMF community structure and diversity, and fresh forage yield. Winter forage crops could increase the spore pool of soil AMF and improve the soil AMF community structure and diversity. Winter Chinese milk vetch in paddy field had the best comprehensive effect on soil physicochemical properties and soil AMF community according to the comprehensive evaluation. These findings provide a theoretical basis for sustainable development and utilization of the southern rice paddy ecosystem.

Comparison of ultra-low lattice thermal conductivity of the full-Heusler compound Li2Rb(Cs)Bi after considering strong quartic anharmonicity

This paper conducts a detailed study on the thermal transport and thermoelectric properties of Li2Rb(Cs)Bi and analyzes the optical phonon frequency shift caused by considering anharmonicity. We mainly focus on studying the microscopic mechanism of the difference in lattice thermal conductivity (κL) of the two materials. By calculating the group velocity, scattering rate, scattering phase space and scattering sub-process, it is concluded that κL is mainly dominated by the acoustic branch. Due to its small group velocity and large scattering rate, Li2CsBi has a low κL, which is 0.60 W m−1K−1 at 300 K. Research results show that n-type Li2CsBi has a higher ZT value of about 2.1 at T = 900 K, while p-type Li2RbBi has a higher ZT value of about 1.5 at the same temperature. These results provide an important theoretical basis for the application of Li2Rb(Cs)Bi in the field of thermoelectric conversion.

A scallop IκB protein involved in innate immunity acts as a key regulator of NF-κB

Chlamys farreri, a commercially important bivalve mollusk, is extensively cultivated in China. In recent years, the frequent occurrence of diseases has led to significant mortality in scallop farms. Despite this, our understanding of scallop's innate immune mechanisms remains limited. The NF-κB signaling pathway plays a crucial role in various biological processes, including cellular, developmental, and immune defense mechanisms. Inhibitors of NF-κB (IκB) proteins block the nuclear localization and DNA binding of NF-κB, thereby inhibiting its activity. However, the role of these proteins in invertebrates is not well understood. In this study, we identified a new homolog of the IκB gene in C. farreri, named CfIκB1. The open reading frame of CfIκB1 spans 1,089 bp, encoding 362 amino acids. Through sequence comparison and phylogenetic analysis, CfIκB1 was classified as a member of the invertebrate IκB family. Quantitative real-time PCR revealed that CfIκB1 transcripts are present in all examined tissues, with the highest expression observed in hemocytes. Expression levels were significantly upregulated following exposure to lipopolysaccharide, peptidoglycan, and polyinosinic:polycytidylic acid. Co-immunoprecipitation studies confirmed that CfIκB1 interacts with NF-κB family proteins CfRel-1 and CfRel. Dual-luciferase reporter assays demonstrated that CfIκB1 inhibits CfRel-dependent activation of NF-κB, ISRE, IFNβ, and AP-1. These findings suggest that CfIκB1 plays a crucial role in regulating NF-κB activity, which is integral to the innate immunity of C. farreri. This research enhances our understanding of the innate immune system in invertebrates and provides a theoretical basis for developing disease-resistant scallops at the molecular level.

How the Smart Energy Can Contribute towards Achieving the Sustainable Development Goal 7

Against the backdrop of the digital technology revolution, smart energy offers significant opportunities to achieve the Energy Sustainable Development Goals (SDG7). However, the theoretical and empirical exploration of how smart energy can achieve SDG7 remains incomplete. This study investigates deeply into the influence and mechanism of smart energy on SDG7. Utilizing China’s smart energy pilot policy, it adopts the synthetic difference-in-difference method and China’s provincial data to estimate the influence of smart energy on SDG7. The results show the following: (1) Smart energy is beneficial to achieving SDG7, and smart energy promotes the realization of SDG7-1 by enhancing energy service accessibility, affordability, and reliability; SDG7-2 by enhancing the renewable energy absorption level and consumption ratio; and SDG7-3 by enabling real-time monitoring, accurate matching, and highly intelligent coordination to improve energy efficiency. (2) Among the quantitative findings, smart energy has notable implications for the SDG7, SDG7-1, and SDG7-3 indexes but has a more limited influence on the SDG7-2 index. (3) The single pilot tests indicate certain differences in effects due to the project scale and program design. Finally, this study provides a theoretical basis and empirical evidence for the region to search for a digital approach to realize SDG7 and smart energy construction planning.

Study on the Influence of Chord Length and Frequency of Hydrofoil Device on the Discharge Characteristics of Floating Matter in Raceway Aquaculture

To investigate the influence of the chord length and frequency of an oscillating hydrofoil device on the discharge characteristics of floating particulate matter, in this study, we take raceway aquaculture as an example and systematically compare and analyze the flow field characteristics of the hydrofoil device with different chord lengths and frequencies, as well as the sewage discharge performance of the raceway based on Computational Fluid Dynamics (CFD). The results indicate that in the particulate matter discharge process of raceway aquaculture, when the chord length and motion frequency of the hydrofoil device are 0.1 W (W is the width of the raceway) and 1.0 Hz, respectively, the anti-Karman vortex streets produced by the hydrofoil device are less affected by the wall, the flow field is the most uniform, the particulate matter discharge performance is the best, and the final floating particulate matter discharge rate reaches up to 99.09%. Adjusting the chord length of the hydrofoil can effectively ameliorate flow field reflux issues, enhancing the uniformity and flow performance of the flow field. When the chord length is 0.1 W, the uniformity of the flow field is optimal. When the chord length is 0.2 W, the flow performance of the flow field is superior. Increasing the frequency enhances the flow performance of the flow field, with an average increase of 0.1 Hz in motion frequency leading to a 19.42% improvement in the average velocity at the outlet. Based on this, we recommend the use of a hydrofoil device with a chord length of 0.1 W and a motion frequency of 1.0 Hz in the raceway aquaculture system to achieve optimal particulate matter discharge performance, providing a theoretical basis and practical guidance for using hydrofoil devices to improve the efficiency of floating particulate matter treatment in raceway aquaculture environments.

Comparative analysis of the nutritional composition and volatile compounds in male and female adults, nymphs, and molts of Eupolyphaga sinensis Walker.

Female adult Eupolyphaga sinensis Walker (FAESW) has traditionally been a food source in Southeast Asian countries such as China and India, due to its rich nutritional content. However, the nutritional value of male adults (MAESW) and its molts (MESW) has hardly been reported. Therefore, this study aims to explore the potential application of MAESW and MESW in food by investigating and comparing their nutritional composition (i.e., protein, amino acids, fatty acids, and essential elements) with traditional sources of nutrition. The protein content of MAESW and MESW was 66.10±0.49% and 59.86±6.07%, respectively, and the highest energy content (462.26±1.28kcal/100g) was observed for MAESW. Eight essential amino acids were determined, of which the males and MESW were found to have higher contents than those of FAESW (p<0.05). Oleic and linoleic acid contents were higher in the adults than nymphs. Moreover, MESW was predominant in calcium (6770.84mg/kg), whereas MAESW was rich in iron (556.12mg/kg). Likened to chicken, the protein, amino acid, fatty acid, and mineral contents of ESW were higher. The volatiles of ESW were related to hexaldehyde, benzaldehyde, acetic acid, and butyric acid. This study provides a better understanding of the chemical composition of ESWs during their growth cycle and helps optimize information on edible insects, promoting their use as a potential food source for humans. As a kind of edible insect, the utilization of adult male Eupolyphaga sinensis Walker (ESW) and its molt is very low at present. Therefore, this study examined the nutrients and volatile substances of ESW (at different growth stages) and molt, which provided a theoretical basis for the subsequent development and utilization of ESW.

A Stepwise Multifactor Regression Analysis of the Interactive Effects of Multiple Climate Factors on the Response of Vegetation Recovery to Drought

In this study, a stepwise multifactor vegetation regression analysis (SMVRA) approach was proposed to investigate the interaction of multiple climate factors on vegetative growth in the study area from 2000 to 2020. It was developed by integrating the stepwise linear regression method, Standardized Precipitation Evapotranspiration Index (SPEI), Normalized Difference Vegetation Index (NDVI), and Pearson correlation coefficient. SMVRA can be used to intuitively understand the interactive effects of multiple correlated factors (e.g., temperature, precipitation, potential evapotranspiration, and the drought index) upon vegetation. The results show that the resilience of vegetation in the BLR basin is influenced by the severity of drought. Annual changes in SPEI over the BLR basin show an increasing trend, with rates of 3.12 × 10−2. Precipitation and NDVI had a strong positive correlation (p &lt; 0.05), found for 34.93% of the total pixels in the study area. In the BLR basin, vegetation growth is inhibited in the 4 years following a drought event. The area near 800 m is most sensitive to drought events. It provides a theoretical basis for future drought response and effective vegetation restoration in the region.

Performance simulation and energy efficiency analysis of multi-energy complementary HVAC system based on TRNSYS

The failure rate and operating cost of the C-B (chiller-gas boiler) system, which has been in use for a long time, are increasing year by year, but there is a lack of reasonable programs and effective measures on how to retrofit the C-B system. In order to explore the prospect of ground-source heat pumps applied to the retrofit of C-B systems in HSCW (hot summer and cold winter) zone, this study designed a G-C-B (GSHP-Chiller-Boiler) system in a partial retrofit context and constructed a TRNSYS numerical simulation model of the retrofit system. Secondly, the performance and energy efficiency of the system is analyzed as well as different control strategies for the ground source heat pump cooling tower are discussed. Finally, the energy conversion efficiency of the chiller is compared based on the energy flow of the system. Studies have shown that the G-C-B system can achieve a seasonal performance factor of 5.3 during the cooling season and 4.1 during the heating season. Compared to the existing C-B system, year-round electricity consumption was reduced by 21 %, gas consumption by 92 %, and combined energy demand by 49.6 %. With the addition of GSHP, the energy conversion efficiency of the chiller has also increased by 21 %. The soil temperature of the G-C-B system increased by less than 0.5 °C after ten years of continuous operation. This paper can provide a valuable theoretical basis for the energy form modification of the C-B system in the HSCW zone.

Carbon Emission Accounting and Reduction Evaluation in Sponge City Residential Areas

This paper aims to establish a more standardized and regulated carbon emission accounting model for sponge cities by unifying the accounting content for carbon emissions and clarifying the relationships between carbon reduction benefits, carbon reduction effects, and carbon sequestration, in order to evaluate the carbon reduction outcomes and mechanisms of sponge city construction. Based on a Life Cycle Assessment (LCA) carbon emission accounting model using the carbon emission factor method, a newly constructed residential area in Tianshui City, Gansu Province, was selected as a case study, and the carbon emission reduction effect of sponge city construction was then investigated. Results indicated that the 30-year full life cycle carbon emissions for sponge city construction in the newly constructed residential area amounted to 828.98 tons, compared to 744.28 tons of CO2 reduction in traditional construction, representing a 47.31% reduction in carbon emissions. Over a 30-year life cycle, this equated to a total carbon emission reduction effect of 1460.31 tons. Additionally, under various rainfall scenarios in a typical year, the carbon emission reduction effect of sponge city construction exceeded the carbon emissions, achieving carbon neutrality within 22 to 30 years of operation. This demonstrates that the carbon emission reduction effect of sponge city communities is significant. The findings of this study provide data and a theoretical basis for the low-carbon construction of sponge cities in China.

Evaluation Method for Voltage Regulation Range of Medium-Voltage Substations Based on OLTC Pre-Dispatch

A new energy industry represented by photovoltaic and wind power has been developing rapidly in recent years, and its randomness and volatility will impact the stable operation of the power system. At present, it is proposed to enrich the regulation of the power grid by tapping the regulation potential of load-side resources. This paper evaluates the overall voltage regulation capability of substations under the premise of considering the impact on network voltage security and providing a theoretical basis for the participation of load-side resources of distribution networks in the regulation of the power grid. This paper proposes a Zbus linear power flow model based on Fixed-Point Power Iteration (FFPI) to enhance power flow analysis efficiency and resolve voltage sensitivity expression. Establishing the linear relationship between the voltages of PQ nodes, the voltage of the reference node, and the load power, this paper clarifies the impact of reactive power compensation devices and OLTC (on-load tap changer) tap changes on the voltages of various nodes along the feeder. It provides theoretical support for evaluating the voltage regulation range for substations. The day-ahead focus is on minimizing network losses by pre-optimizing OLTC tap positions, calculating the substation voltage regulation boundaries within the day, and simultaneously optimizing the total reactive power compensation across the entire network. By analyzing the calculated examples, it was found that a pre-scheduled OLTC (on-load tap changer) can effectively reduce network losses in the distribution grid. Compared with traditional methods, the voltage regulation range assessment method proposed in this paper can optimize the adjustment of reactive power compensation devices while ensuring the voltage safety of all nodes in the network.

Histological observations and transcriptome analyses reveal the dynamic changes in the gonads of the blotched snakehead (Channa maculata) during sex differentiation and gametogenesis

BackgroundBlotched snakehead (Channa maculata) displays significant sexual dimorphism, with males exhibiting faster growth rates and larger body sizes compared to females. The cultivation of the all-male population of snakeheads holds substantial economic and ecological value. Nonetheless, the intricate processes governing the development of bipotential gonads into either testis or ovary in C. maculata remain inadequately elucidated. Therefore, it is necessary to determine the critical time window of sex differentiation in C. maculata, providing a theoretical basis for sex control in production practices.MethodsThe body length and weight of male and female C. maculata were measured at different developmental stages to reveal when sexual dimorphism in growth initially appears. Histological observations and spatiotemporal comparative transcriptome analyses were performed on ovaries and testes across various developmental stages to determine the crucial time windows for sex differentiation in each sex and the sex-related genes. Additionally, qPCR and MG2C were utilized to validate and locate sex-related genes, and levels of E2 and T were quantified to understand sex steroid synthesis.ResultsSexual dimorphism in growth became evident starting from 90 dpf. Histological observations revealed that morphological sex differentiation in females and males occurred between 20 and 25 dpf or earlier and 30–35 dpf or earlier, respectively, corresponding to the appearance of the ovarian cavity or efferent duct anlage. Transcriptome analyses revealed divergent gene expression patterns in testes and ovaries after 30 dpf. The periods of 40–60 dpf and 60–90 dpf marked the initiation of molecular sex differentiation in females and males, respectively. Male-biased genes (Sox11a, Dmrt1, Amh, Amhr2, Gsdf, Ar, Cyp17a2) likely play crucial roles in male sex differentiation and spermatogenesis, while female-biased genes (Foxl2, Cyp19a1a, Bmp15, Figla, Er) could be pivotal in ovarian differentiation and development. Numerous biological pathways linked to sex differentiation and gametogenesis were also identified. Additionally, E2 and T exhibited sexual dimorphism during sex differentiation and gonadal development. Based on these results, it is hypothesized that in C. maculata, the potential male sex differentiation pathway, Sox11a–Dmrt1–Sox9b, activates downstream sex-related genes (Amh, Amhr2, Gsdf, Ar, Cyp17a2) for testicular development, while the antagonistic pathway, Foxl2/Cyp19a1a, activates downstream sex-related genes (Bmp15, Figla, Er) for ovarian development.ConclusionsThis study provides a comprehensive overview of gonadal dynamic changes during sex differentiation and gametogenesis in C. maculata, establishing a scientific foundation for sex control in this species.

The Role of Strategic Human Resources Management in Developing Competitive Advantage: a Systematic Literature Review, synthesis and framework for Future Research

Purpose - Research examining the role of Strategic Human Resources Management (SHRM) in increasing Competitive Advantage (CA). There are many studies of SHRM and CA, but the role of SHRM is still in doubt and there are various modifications of research variations that test mediating variables and or moderating variables that affect CA, and there are differences in the theoretical basis used in various studies. To address this gap, a literature review of the role of SHRM on CA is presented. Design/methodology/approach – Systematic review protocols and content analysis was used to analyze and synthesize insights from 50 empirical studies obtained from two academic databases: Scopus, Google Scholar. Findings – Significant diversity in previous research on the role of SHRM on CA is summarized in several themes. There are significant limitations in the conceptualization of the influence of SHRM on CA, including along with a narrow focus on geographic, methodological and contextual foci of prior studies. The authors propose a comprehensive framework and extensive gap-specific research directions to aid future research. Research limitations/implications – The SLR is limited in its consideration of empirical studies published in academic journal articles obtained from Scopus, and Google Scholar databases. Social implications – The authors reveal the foundation variables, theories that can be used in future research on the influence of SHRM on SCA. Originality/value – This study enhances and extends the research on SHRM's influence on CA by utilizing the perspectives of various modal and multidisciplinary variables for further investigation. The use of perspectives can further ascertain the role of SHRM on CA.