Their Total Phenolic Content Research Articles

Phenolic Profile of Peanut By‐products: Antioxidant Potential and Inhibition of Alpha‐Glucosidase and Lipase Activities

AbstractFree, esterified, etherified and insoluble‐bound phenolics from three samples, namely meal from whole peanuts (MWP), meal from dry‐blanched peanuts (MDBP) and peanut skin (PS) were evaluated for their total phenolic content (TPC), and phenolic profile using HPLC–DAD–ESI–MSn. The TPC was in the order of PS > MWP > MDBP. Among 37 compounds identified 10 were reported for peanuts for the first time. Proanthocyanidins were detected in peanut skin and MWP, but not in MDBP. The presence of peanut skin in MWP rendered up to 9.8‐fold higher scavenging activity towards the DPPH radical as compared with MDBP. Phenolics from all samples and fractions showed inhibition of alpha‐glucosidase and lipase activities, which are key enzymes involved in the absorption of glucose and triacylglycerols, respectively. Therefore, their antioxidant properties as well as their potential role in the management of diabetes and obesity makes them worthy of further consideration as functional food ingredients.

Total Phenolic Contents and Antioxidant Activities of Selenium-Rich Black Tea Versus Regular Black Tea

Two black aqueous tea extracts of Camellia sinensis, (selenium-rich black tea (Se-BTE) and regular black tea (R-BTE)) were assessed for their total phenolic content (TPC) and antioxidant properties. Aqueous tea extracts were prepared using different extraction temperature and time. TPC was measured using the Folin-Ciocalteu method, while antioxidant activity was measured by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The R-BTE showed significantly higher TPC (P < 0.05) and antioxidant activity (P < 0.05) than the Se-BTE, which may be due to these being different cultivars, or differences in growing conditions. Our findings demonstrate that both extraction temperature and time are important determinants of extracted TPC and antioxidant activities of these teas. In addition and in contrary to our expectations, the high level of organic selenium in black tea did not enhance the antioxidant activity when compared with black tea containing very low level of organic selenium. Other elements and minerals may contribute to the high antioxidant activity detected in the regular black tea.

Optimization of microwave vacuum drying parameters for germinated lentils based on starch digestibility, antioxidant activity and total phenolic content

The aim of this study was to optimize the processing parameters of pulse mode microwave-vacuum drying of germinated green and red lentils (CDC Greenland and CDC Maxim) and investigate the changes in their total phenolic content (TPC), total antioxidant activity (TAA) and in-vitro starch digestibility (SD). The lentils were germinated for 5 days and dried by a pulse mode microwave-vacuum method, using 2 s to 8 s out of 10 s pulsed mode at 2000W microwave power and varying the vacuum pressure level between 15 and 45 kPa. In-vitro starch digestibility increased significantly with increased microwave power level. The TPC and TAA appeared to vary distinctively in the two varieties of selected lentils. Vacuum pressure levels did not significantly (p>0.05) affect any responses. Green lentils could be dried at 8 s microwave power and 45 kPa vacuum pressure and red lentils could be dried at 5.5 s microwave power and 42.19 kPa vacuum pressure. The microwave-vacuum drying showed great potential for the drying of germinated lentils.

Optimization of microwave vacuum drying parameters for germinated lentils based on starch digestibility, antioxidant activity and total phenolic content

The aim of this study was to optimize the processing parameters of pulse mode microwave-vacuum drying of germinated green and red lentils (CDC Greenland and CDC Maxim) and investigate the changes in their total phenolic content (TPC), total antioxidant activity (TAA) and in-vitro starch digestibility (SD). The lentils were germinated for 5 days and dried by a pulse mode microwave-vacuum method, using 2 s to 8 s out of 10 s pulsed mode at 2000W microwave power and varying the vacuum pressure level between 15 and 45 kPa. In-vitro starch digestibility increased significantly with increased microwave power level. The TPC and TAA appeared to vary distinctively in the two varieties of selected lentils. Vacuum pressure levels did not significantly (p>0.05) affect any responses. Green lentils could be dried at 8 s microwave power and 45 kPa vacuum pressure and red lentils could be dried at 5.5 s microwave power and 42.19 kPa vacuum pressure. The microwave-vacuum drying showed great potential for the drying of germinated lentils.

Extractable and non-extractable bound phenolic compositions and their antioxidant properties in seed coat and cotyledon of black soybean (Glycinemax (L.) merr)

Both extractable (EPE) and non-extractable bound phenolic (BPE) in the seed coat and cotyledon of black bean were investigated in the present study. Their total phenolic contents (TPC) and antioxidant activities (DPPH, ABTS and FRAP) were evaluated, and the phytochemical compositions of EPE and BPE in both seed coat and cotyledon of black soybean were identified and quantified (Glycinemax(L.) merr) by UPLC-ESI-QTOF-MS/MS and UPLC-ESI-QqQ-MS/MS. In the present study, a total of 112 and 78 compounds were identified or tentatively characterized in the black soybean EPE and BPE, respectively, with the majority being phenolic acids and flavonoids. Seed coat contains more abundant phenolics than that of cotyledon in all fractions. In addition, our results indicated that acid hydrolysis is more thorough than alkaline hydrolysis in the extraction of bound phenolics of black soybean. Our study provides available information for non-extractable bound phenolic constituents and their distribution in the seed coat and cotyledon of black soybean.

Comparison of Different Extraction Methods for the Determination of the Antioxidant and Antifungal Activity ofCynara ScolymusandC. CardunculusExtracts and Infusions

Extracts and infusions of wild artichoke (Cynara cardunculus L.) and globe artichoke (C. scolymus L.) (heads, bracts and stems) were examined for their total phenolic content (TPC) and their antioxidant activity after performing Classical Extraction (CE) and Ultrasound-Assisted Extraction (UAE). UAE proved to be more effective, since extracts exhibited higher antioxidant activity and TPC values than CE extracts and infusions. Moreover C. cardunculus heads extract using UAE, displayed the maximum TPC values (1.57 mg gallic acid equivalents (GAE) g⁻¹ fresh weight (fw)), the highest DPPH· scavenging activity (IC₅₀; 0.91mg mL⁻¹) and the highest ABTS·+ radical scavenging capacity (2.08 mg Trolox Equivalents (TE) g⁻¹ fw). Moreover, the effect of different concentrations of C. cardunculus head extracts (showing the highest TPC and antioxidant activity) on Aspergillus parasiticus growth was estimated in AFPA medium. The maximum inhibition was found to be 42.1% in comparison with the control.

ANTI-CANCER ACTIVITY OF ASTER TATARICUS ON SCC-9 HUMAN ORAL SQUAMOUS CARCINOMA.

Background:Ephedra is among Palestinian medicinal plants that are traditionally used in folkloric medicine for treating many diseases. Ephedra is known to have antibacterial and antioxidant effects. The goal of this study is to evaluate the antioxidant activity of different extracts from the Ephedra alata plant growing wild in Palestine, and to analyze their phenolic and flavonoid constituents by HPLC/PDA and HPLC/MS.Materials and Methods:Samples of the Ephedra alata plant grown wild in Palestine were extracted with three different solvents namely, 100% water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (AA), as well as phenolic and flavonoids content by HPLC/PDA/MS.Results:The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA of extracts. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by 100% ethanol, and finally with 100% water. TFC however was highest in the following order: 100% ethanol > 80% ethanol > water. Pearson correlation indicated that there is a significant correlation between AA and TPC, but there is no correlation between AA and TFC. Simultaneous HPLC-PDA and UHPLC-MS analysis of the ethanolic plant extracts revealed the presence of Luteolin-7-O-glucuronide flavone, Myricetin 3-rhamnoside and some other major polyphenolic compounds that share myricetin skeleton.Conclusion:Ephedra alata extract is rich in potent falvonoid glycosidic compounds as revealed by their similar overlaid UV-Vis spectra and UHPLC-MS results. On the basis of these findings, it is concluded that Ephedra alata constitutes a natural source of potent antioxidants that may prevent many diseases and could be potentially used in food, cosmetics, and pharmaceutical products.

Maturational effects on phenolic constituents, antioxidant activities and LC-MS / MS profiles of lemon ( Citrus limon ) peels

Methanol extracts from peels of lemon ( Citrus limon (L.) Burm) fruits collected at three maturity stages (immature, half-mature and mature) were analyzed for their total phenolic contents (TPC) and antioxidant activities. Irrespective to peel parts analyzed (albedo or flavedo), a declining trend was observed for TPC during maturation. By using DPPH, ABTS and FRAP assays, it was found that all extracts had strong antioxidant activities. As maturation progress, the antioxidant activity of both peel parts decreased. Significant relationships between antioxidant capacity and TPC were found, indicating on the one hand, that phenolic compounds are the major contributors to the antioxidant properties, and that the three methods have similar predictive capacity for antioxidant activity of lemon peel, on the other hand. Analysis by HPLC-DAD-MS/MS revealed that flavedo and albedo extracts haves similar phenolic profiles and allowed the identification of hesperidin, rutin, eriocitrin, diosmin and hyperoside, as the main compounds.

In vitro antioxidant, antimicrobial and cytotoxic activities and green biosynthesis of silver & gold nanoparticles using Callistemon citrinus leaf extract

The current study was aimed to synthesis and characterization of silver (AgNPs) & gold (AuNPs) nanoparticles using Callistemon citrinus leaf extract, and to evaluate their in vitro antioxidant, antimicrobial, cytotoxic activities as well as their total phenolic content (TPC). Silver and gold were synthesized and characterized via UV-vis absorbance spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction (XRD) analyses. The antioxidant activity was evaluated using dot-blot and DPPH staining, and via phosphomolybdenum assays. Also, the in vitro antimicrobial activity was evaluated via disc agar plate method. The cytotoxic activity was evaluated via brine shrimp lethality test (BSLT), and TPC was estimated via Folin-Ciocalteu’s assay. The transmission electron microscopy (TEM) analysis showed that the sizes of the synthesized AgNps ranged from 8-14 nm with maximum UV/vis absorbance at 450nm. Also, the synthesized AuNPs exhibited an average size of 5.8 to 8.84nm with maximum UV/vis absorbance at 535nm. Moreover, the results revealed that TPC of the tested extracts was ranged from 548.85 to 123.30 mg gallic acid equivalent (GAE)/g dry extract. The total antioxidant capacity (TAC) was ranged from 643.90 to 147.96 mg ascorbic acid equivalent/g dry extract. Furthermore, there is a promising antimicrobial activity against four strains with inhibition zones ranged from 8.5 to 15.5mm, Penicillin G was used as positive control at concentration of 100 μg/disc. In terms of LC50 the n-butanol extract (63.09 μg/ml) was the most potent cytotoxic, followed by EtOAc (100.0 μg/ml). In, conclusion the leaves of Callistemon citrinus showed a noticeable antioxidant, antimicrobial & cytotoxic activities and the ability to produce AgNPs and AuNPs.

Influence of pretreatment and modifiers on subcritical water liquefaction of spent coffee grounds: A green waste valorization approach

Spent coffee ground (SCG) is the main residue of the coffee industry with a worldwide annual generation of 6 million ton. SCG contains large amounts of organic compounds (i.e., polyphenols, lignin, cellulose, hemicellulose, and other polysaccharides) that can be exploited as a source of value-added products. Effective valorization of SCG depends on the extraction methods. Therefore, the aim of this study was to investigate the influence of pretreatments such as microwave and ultrasonic and modifiers such as nitrogen and carbon dioxide on subcritical water (SCW) liquefaction of SCG at different temperatures (180–240 °C) and pressures (20–60 bar). The hydrolysate was studied for their total phenolic content (TPC), total flavonoids content (TFC), reducing sugars (RSs), protein content antioxidants, and antimicrobial activities. The extracts showed that the TPC value ranged from 33.1 ± 1.89 to 51.2 ± 1.87 and the TFC value ranged from 15.13 ± 1.73 to 25.51 ± 1.55 mg/g of GA and CE respectively. The RSs and protein content ranged from 8.88 ± 1.19 to 37.9 ± 4.21 g/100 g and 20.05 ± 2.33 to 47.38 ± 4.41 respectively. DPPH and ABTS+ radical scavenging antioxidant activities ranged from 0.36 ± 0.03 to 0.51 ± 0.08 and from 0.39 ± 0.04 to 0.76 ± 0.01 mmol TE/g, respectively. The extracts also showed very good antimicrobial activity against common foodborne pathogens with Staphylococcus aureus being the most sensitive bacteria having an MIC value of 5 mg/ml lyophilized extracts. This study showed that pretreatment and the use of modifiers could increase the liquefaction of SCG and composition of the extracts by reducing the severity index.

Phytochemical profiles and biological activities of Curcuma species subjected to different drying methods and solvent systems: NMR-based metabolomics approach

Curcuma species (Zingiberaceae) have been used in traditional medicine in India and Southeast Asia to treat many human ailments and are believed to possess many biological activities. In this study, four Curcuma species (C. zedoaria, C. xanthorrhiza, C. aeruginosa and C. mangga) were compared in terms of their total phenolic contents (TPC), and bioactivities including free radical scavenging, α-glucosidase and nitric oxide (NO) inhibition. Their phytochemical constituents were also determined by a proton nuclear magnetic resonance (1H NMR) based metabolomics approach. The species were dried using three drying methods (air, freeze and oven) and extracted with two ethanol ratios (50 and 100%). The correlations between TPC and bioactivities, with phytochemical constituents were obtained using partial least square (PLS) regression. The results showed that C. xanthorrhiza had the highest TPC, antioxidant and NO inhibitory activities, whereas C. mangga showed the highest α-glucosidase inhibitory activity. Notable and clear separations among the four species and the three drying methods for C. xanthorrhiza and C. mangga were revealed by principal component analysis (PCA). The PCA scores plot showed close relationship between C. xanthorrhiza and C. mangga, and were separated from C. zedoaria and C. aeruginosa. The PLS model indicated that curcumin, xanthorrhizol, 1-(4-hydroxy-3,5-dimethoxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-(lE,6E)-1,6-heptadiene-3,4-dione and demethoxycurcumin were significantly correlated with the higher antioxidant and NO inhibitory activities as demonstrated by C. xanthorrhiza. Meanwhile, zerumin B, dehydrocurdione, (E)-labda-8(17),12-diene-15,16-dial, calcaratarins A and curcuzederone were present in C. mangga contributed to the α-glucosidase inhibitory activity. The freeze dried samples of all the absolute ethanol extracts of Curcuma species exhibited significant bioactivities with the highest contents of metabolites.

Angiotensin I-converting enzyme (ACE) inhibitory activity of Fucus spiralis macroalgae and influence of the extracts storage temperature—A short report

Recently, increasing attention has been paid to the marine algae as a natural source of novel angiotensin-I converting enzyme (ACE) inhibitors, such as the phlorotannins that are the predominant polyphenols in brown algae. This study reports, for the first time, the ACE inhibition of methanol extract/fractions from Azorean brown algae Fucus spiralis (Fs) determined by HPLC-UV method, their total phenolic content (TPC) quantified as phloroglucinol equivalents (PE) and the effect of the Fs dry powder methanol extracts (Fs-DME) storage temperature on ACE inhibition. The results indicate that the ACE inhibition of Fs-DME decreased by 28.8% and 78.2% when stored during 15days at −80°C and −13°C, respectively, as compared with the activity of Fs-DME at a refrigerated temperature of 6°C and assayed immediately after extraction that showed a value of 80.1±2.1%. This Fs-DME sample was fractionated by ultrafiltration membranes into three molecular weight ranges (<1kDa, 1–3kDa and >3kDa), presenting the fraction>3kDa remarkably high ACE inhibition (88.8±2.4%), TPC value (156.6±1.4mg PE/g of dry weight fraction) and yield. Furthermore, chromatographic and spectrophotometric analyses corroborate that phenolic compounds were present in Fs methanol extract/fractions, and also revealed that phloroglucinol occurs in Fs. The results seem to suggest that Azorean Fs can be a source of powerful ACE-inhibitory phlorotannins with potential impact on public health, particularly on hypertensive patients.

Comparison of phenolic content and antioxidant activities of millet varieties grown in different locations in Sri Lanka.

Soluble and bound phenolic compounds were extracted from different varieties of millet types namely, finger millet, foxtail, and proso millet cultivated at dry and intermediate climatic zones in Sri Lanka. The extracts were examined for their total phenolic content (TPC), total flavonoid content (TFC), and proanthocyanidin content (PC). The antioxidant activities were meassured by reducing power (RP), trolox equivalent antioxidant capacity (TEAC), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activity, ferrous ion chelating ability (FICA), and using a β carotene linoleate model system. The ferulic acid content of extracts were determined using high‐performance liquid chromatoghraphy (HPLC). Finger millet showed the highest phenolic content and antioxidant activities compared to proso and foxtail millets. The phenolic content as well as antioxidant activites of soluble and bound phenolic extracts of millets were affected by variety and cultivated location. The highest phenolic content and antioxidant activites were reported for millet samples cultivated in areas belonging to the dry zone in Sri Lanka.

Αntioxidant activity of Cynara scolymus L. and Cynara cardunculus L. extracts obtained by different extraction techniques

Extracts of different parts (heads, bracts and stems) of Cynara cardunculus L. (cardoon) and Cynara scolymus L. (globe artichoke), obtained by two different extraction techniques (Ultrasound-Assisted Extraction (UAE) and classical extraction (CE)) were examined and compared for their total phenolic content (TPC) and their antioxidant activity. Moreover, infusions of the plant’s parts were also analysed and compared to aforementioned samples. Results showed that cardoon’s heads extract (obtained by Ultrasound-Assisted Extraction) displayed the highest TPC values (1.57 mg Gallic Acid Equivalents (GAE) g−1 fresh weight (fw)), the highest DPPH• scavenging activity (IC50; 0.91 mg ml−1) and the highest ABTS•+ radical scavenging capacity (2.08 mg Trolox Equivalents (TE) g−1 fw) compared to infusions and other extracts studied. Moreover, Ultrasound-Assisted Extraction technique proved to be more appropriate and effective for the extraction of antiradical and phenolic compounds.

Physicochemical, functional and antioxidant properties of flour from pearl millet varieties grown in India

Indian pearl millet varieties were selected, milled to flour and studied for their proximate composition, hunter color, functional, and antioxidant properties. Proximate composition and hunter color values differed significantly (p < 0.05) among varieties. Hunter L* value was found the highest for HHB-223 flour whereas a*, b* and ΔE was the highest for W-445 flour. Flours also showed significant variations in their total phenolic content (TPC), total flavonoids content (TFC), antioxidant activity (DPPH) and metal chelating activity. Variety GHB-732 showed the highest values for TPC (3137 µg GAE/g) and DPPH (46.7 %) activity. TFC was, however, found the highest for HC-10 (2484 µg CE/g) variety. Several significant correlations were observed among different flour properties as revealed by both pearson correlations and principal component analysis. Formulation of chapatti resulted in decrease in TPC, TFC, DPPH values when compared to its flour. However, reverse was observed for metal chelating activity.

Comparative Study of the Antioxidant Activity of Two Popular Green Tea Beverages Available in the Local Market of Saudi Arabia

Antioxidants have numerous applications due to their multiple roles in diminishing harmful effects of oxidative stress. The objective of this work was to highlight the importance of green tea by evaluating the antioxidant activity of the most popular green tea brands in Saudi Arabia, Lipton and Rabea. To our knowledge, no studies have so far been done to estimate the antioxidant activity of these brands. To determine the antioxidant activities of these two brands, 10 mg/ml of each brand was extracted and their total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide scavenging activity, ferric reducing power and ferrous ion chelating effect were measured. The TPC of Lipton tea was 678.7 υg of gallic acid equivalents (GAE)/10mg, whereas in Rabea tea, the TPC was 647.1 υg GAE/10mg. The presence study indicated that there were no significant differences in total phenolic contents and the percentage inhibition as shown in DPPH and H2O2 assays among Lipton and Rabea green teas. Moreover, it was found that all assays have exhibited high antioxidant activity in both green teas. In conclusion, our study showed evidence for evenness and stability of the antioxidant activity of the two commercial green teas available in the markets of Saudi Arabia. Continued researches are needed to further the current knowledge on the health-promoting effects of this popular beverage using different supplements by different mechanisms.

Comparative Analysis of Nutritional and Bioactive Properties of Aerial Parts of Snake Gourd (Trichosanthes cucumerina Linn.).

The present investigation was carried out to determine the nutritional and functional properties of T. cucumerina. Water extracts of freeze dried flowers, fruits, and leaves of T. cucumerina were evaluated for their total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, α-amylase inhibitory activity, and fiber and mineral contents. Antioxidant activity, TPC, and TFC were significantly higher (P ≤ 0.05) in leaves than in flowers and fruits. A significant linear correlation was observed between the TPC, TFC, and antioxidant activities of plant extracts. Although, leaves and flower samples showed a significantly higher (P ≤ 0.05) amylase inhibitory activity than the fruit samples, the overall amylase inhibition was low in all three parts of T. cucumerina. Soluble and insoluble dietary fiber contents were significantly higher (P ≤ 0.05) in fruits than in flowers and leaves. Ca and K contents were significantly higher (P ≤ 0.05) in leaf followed by fruit and flower and Mg, Fe, and Zn contents were significantly higher (P ≤ 0.05) in leaves followed by flowers and fruits. In conclusion, T. cucumerina can be considered as a nourishing food commodity which possesses high nutritional and functional benefits for human health.

Anticancer Activity, Antioxidant Activity, and Phenolic and Flavonoids Content of Wild Tragopogon porrifolius Plant Extracts.

Tragopogon porrifolius, commonly referred to as white salsify, is an edible herb used in folk medicine to treat cancer. Samples of Tragopogon porrifolius plant grown wild in Palestine were extracted with different solvents: water, 80% ethanol, and 100% ethanol. The extracts were analyzed for their total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AA). Four different antioxidant assays were used to evaluate AA of the extracts: two measures the reducing power of the extracts (ferric reducing antioxidant power (FRAP) and cupric reducing antioxidant power (CUPRAC)), while two other assays measure the scavenging ability of the extracts (2,2-azino-di-(3-ethylbenzothialozine-sulphonic acid (ABTS)) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)). Anticancer activity of the plant extracts were also tested on HOS and KHOS osteosarcoma cell lines. The results revealed that the polarity of the extraction solvent affects the TPC, TFC, and AA. It was found that both TPC and AA are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. TFC however was the highest in the following order: 80% ethanol > 100% ethanol > water. The plant extracts showed anticancer activities against KHOS cancer cell lines; they reduced total cell count and induced cell death in a drastic manner.

Biofunctional Activities of Sanguisorbae officinalis L. Leaves Ethanol Extract

This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic compounds, and antioxidative activities using various in vitro assay such as DPPH, ABTS radical scavenging activity, FRAP activity, and reducing power. The TPC and TFC were found to be 119.3±1.54 mg gallic acid and 59.6±1.43 mg rutin at mg of 70% ethanol extracts, respectively. Catechin was the major material among the phenolic compounds in SO extracts. The DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP activity, and reducing power of SO extracts were increased in a dose-dependent manner. These results suggest that SO extracts could be considered as a good source of natural ntioxidants and functional food ingredient.

Phenolic content distribution and antioxidant activities of &lt;i&gt;Terminalia sericea&lt;/i&gt; Burch

Background: Terminalia sericea has been used traditionally for the treatment of diseases associated with oxidative stress. This study was aimed at determining the distribution of phenols in the leaves, stem bark and root bark of Terminalia sericea and their antioxidant activity.Materials and methods: Hot and cold water, methanol/acetone extracts were evaluated for their total phenolic content (TPC), flavone/flavonol content (FFC), flavonone/dihydroflavone content (FDFC), hydroxycinnamic acid derivative content (HCAC) and tannin content (TC). DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals and reducing power assays were used to assess the antioxidant activity.Results: The leaves had the highest average TPC (440) expressed in milligram Gallic Acid Equivalent (mgCAE) /gram of the extract. The leaves also had the highest average TC (7.14) expressed in milligram Quercetin Equivalent (mgQE) /gram of the extract. The stem had the highest average FDFC (19.23 mgQE/g) while the root had the highest average FFC (74.76 mgQE/g) and HCAC (214.57) expressed in milligram Caffeic Acid Equivalent mgCAE/ gram of the extract. The stem exhibited the highest average DPPH free radical scavenging (9.85 μg/mL) and reducing power (6.01 μg/mL) activities. Water was a better extracting solvent for TPC and FDFC while methanol/acetone was a better extracting solvent for FFC and HCAC. The correlation between TC and reducing power activity (r=0.668) at P &lt; 0.05 suggests that tannins were responsible for the antioxidant activity.Conclusion: This study has shown that the distribution of phenolics differs in the organs of T. sericea, and could affect the quality of medicinal products sold.Key words: Phenolic contents; Terminalia sericea; Antioxidant activity; Distribution studies.