Thawing Stage Research Articles

The variation characteristics of micro-pore structures of underground rocks in cold regions subject to freezing and thawing cycles

In order to investigate the evolution characteristics of the microscopic pore structures of the rocks in the underground cavities subject to freezing and thawing cycles, in this article, we conducted several rounds of freezing and thawing cyclic tests and detected the rock samples after each round of freezing and thawing cycles by means of nuclear magnetic resonance (NMR) and CT scanning. Based on the acquired NMR T2 relaxation time spectra, porosity variations, pore distributions, and imaging results of the rock samples at different freezing and thawing stages, the effects of the number of freezing and thawing cycles on the rock’s NMR T2 spectra, porosity, and microscopic pore structures were analyzed. Results show that, for the rock sample collected from the underground cavities, the micro-pore structures were significantly affected by the number of freezing and thawing cycles. More freezing and thawing cycles resulted in larger porosity and higher damage degree of the rock samples. The present study helps to unveil the evolution characteristics of the micro-pore structures of the rock samples in underground cavities after freezing and thawing cycles, and thus provides theoretical basis for the damage mechanisms and stability analysis of underground rocks in cold regions.

Isotopic tracing of hydrologic drivers including permafrost thaw status for lakes across Northeastern Alberta, Canada: A 16-year, 50-lake assessment

Study regionSurveys of stable isotopes of water in 50 boreal lakes were conducted during 2002–2017 as a component of Alberta’s Oil Sands acid sensitivity program in northeastern Alberta. Study focusUsing an isotope mass balance approach, watershed, climatic and isotopic data were applied to estimate evaporation losses and residence time of lakes, as well as to estimate water yield from watersheds. New hydrological insights for the regionSite-specific differences in water yield to 50 lakes over 16 years were found to be controlled by latitudinal gradients in climate, wetland type, lake/watershed configuration and permafrost. 19 plateau watersheds located northeast of Fort McMurray and in the Birch and Caribou Mountains which contained significant permafrost were found to have similar water yield to permafrost-poor watersheds if fen-dominated (159 mm/yr: n = 5 vs. 166 mm/yr; n = 31), and enhanced water yield (405 mm/yr; n = 14) if bog-dominated. Water yield was found to be systematically dependent on permafrost extent, yielding up to several hundred millimetres of additional runoff in bog-dominated systems. Temporal trend analysis indicates systemic momentum of change in hydrologic drivers over the 16-year period, although few are statistically significant. A new conceptual framework is proposed for classification of site-to-site permafrost thaw stage to improve water yield prediction, which is expected to influence lake water quality including observed pH increases noted previously for many lakes in the region.

Physiological response of Secale cereale L. seedlings under freezing-thawing and alkaline salt stress.

Freezing-thawing and saline-alkaline are the major abiotic stress for the pasture in most high-latitude areas, which are serious threats to the yield of pasture. In this study, the osmotic adjustment substances, membrane lipid peroxidation, and antioxidant enzymes activities of rye (Secale cereale L., cv. Dongmu-70) seedlings under different treatments: CK (no treatment), SC (Na2CO3 treatment), FT (freezing-thawing treatment), and FT+SC (combined Na2CO3 and freezing-thawing treatments), were investigated. At the freezing stage, the content of MDA and proline, the activity of APX, SOD, and POD increased with the decrease of the temperature in the leaves of rye seedlings in FT and FT+SC treatments and reached the maximum value at - 5 °C. In addition, the content of protein and H2O2, CAT activity reached the maximum value at 0 °C; the damage is larger under low temperature stress at 0 °C and - 5 °C in rye seedling. At the thawing stage, the content of MDA and H2O2 in seedling leaves decreased in FT and FT + SC treatments. These results demonstrated that proline content and antioxidant enzymes activities could play an important role in protecting cytomembrane and scavenging ROS respectively in rye under alkaline salt stress and freezing-thawing stress. The result also indicated rye seedlings were subjected to a freezing-thawing stress which resulted in a reversible (recoverable) injury.

Contribution of Peatland Permafrost to Dissolved Organic Matter along a Thaw Gradient in North Siberia.

Permafrost peatlands are important carbon stocks currently experiencing rapid evolution after permafrost thaw. Following thaw, dissolved organic matter (DOM) is a potentially important pathway for the release of permafrost carbon. This study investigates the origin and composition of DOM across sites at different stages of thaw in a discontinuous permafrost area of North Siberia. We determine the optical properties, molecular composition, and stable isotopic (δ13C) and radiocarbon (14C) contents of DOM. Early stages of thaw are characterized by high DOC concentrations, high aromaticity, contribution of vegetation-derived DOM, and a high contribution of permafrost carbon. In contrast, in later stages, the microbial contribution to DOM increases, and only modern carbon is detected. This work links DOM composition with its radiocarbon content in permafrost peatlands. It shows that DOM originating from previously frozen permafrost peatlands is highly aromatic and previously processed. It highlights the variability of post-thaw carbon dynamics in boreal and arctic ecosystems.

Biochemical analyses of Dendrobium Sabin Blue PLBs during cryopreservation by vitrification.

Dendrobium Sabin Blue is an important orchid hybrid that has been grown extensively as cut flower, potted plant and isalso popular for its deep purplish blue flowers. The most efficient long term conservation method of this hybrid is through cryopreservation. Cryopreservation involving the vitrification method consists of explants exposure to highly concentrated cryoprotective solution followed by freezing rapidly in liquid nitrogen. However, these treatments involved highly concentrated cryoprotectant that could incur toxicity to the explants. Hence, cryopreservation protocol requires biochemical analyses in understanding the damages or injuries occurred duringcryopreservation treatments. In this study, biochemical analyses revealed a general reduction in chlorophyll, carotenoid and porphyrin content to 0.40µg/gFW (thawing stage), 31.50µg/gFW unloading stage and 2230.41µg/gFW (thawing stage), respectively in comparison to the control treatments. In addition, increased level in proline content were obtained at different cryopreservation stages with highest level (5.42 µmole/g F W)recorded at the PVS2 dehydration stage. Fluctuated outcomes were obtained in catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) enzyme activities in PLBs exposed to different cryopreservation stages. Lowest values recorded for CAT enzyme activity were obtained at the dehydration stage (3.94U/g). Lowest POX enzyme activities were obtained at the dehydration (122.36U/g) and growth recovery (106.40U/g) stages. Additionally, lowest APX enzyme activities values were recorded at the thawing (7.47U/g) and unloading (7.28U/g) stages. These have contributed to low regenerationofDendrobium Sabin Blue protocorm like bodies (PLBs) following cryopreservation. Hence, in the future experimental design,exogenous antioxidant could be includedin the cryopreservation procedures to improve the existing protocol.

Hierarchical classification of snowmelt episodes in the Pyrenees using seismic data.

In recent years the analysis of the variations of seismic background signal recorded in temporal deployments of seismic stations near river channels has proved to be a useful tool to monitor river flow, even for modest discharges. The objective of this work is to apply seismic methods to the characterization of the snowmelt process in the Pyrenees, by developing an innovative approach based on the hierarchical classification of the daily spectrograms. The CANF seismic broad-band station, part of the Geodyn facility in the Laboratorio Subterráneo de Canfranc (LSC), is located in an underground tunnel in the Central Pyrenees, at about 400 m of the Aragón River channel, hence providing an excellent opportunity to explore the possibilities of the seismic monitoring of hydrological events at long term scale. We focus here on the identification and analysis of seismic signals generated by variations in river discharge due to snow melting during a period of six years (2011–2016). During snowmelt episodes, the temporal variations of the discharge at the drainage river result in seismic signals with specific characteristics allowing their discrimination from other sources of background vibrations. We have developed a methodology that use seismic data to monitor the time occurrence and properties of the thawing stages. The proposed method is based on the use of hierarchical clustering techniques to classify the daily seismic spectra according to their similarity. This allows us to discriminate up to four different types of episodes, evidencing changes in the duration and intensity of the melting process which in turn depends on variations in the meteorological and hydrological conditions. The analysis of six years of continuous seismic data from this innovative procedure shows that seismic data can be used to monitor snowmelt on long-term time scale and hence contribute to climate change studies.

Short- and Long-Term Effects of Snow-Depth on Korean Pine and Mongolian Oak Litter Decomposition in Northeastern China

Changes in snow-depth induced by climate change can affect litter decomposition, but it remains unclear whether the effects of snow-depth on litter decomposition vary among seasons. Our aim was to assess the possible short- and long-term effects of snow-depth on foliar litter decomposition for Korean pine (Pinus koraiensis) and Mongolian oak (Quercus mongolica). A snow-depth manipulation experiment that included snow-removal, control and snow-addition treatments was conducted in northeastern China. All the snowfall was intercepted by transparent plexiglass in the snow-removal treatment and then transferred to the snow-addition treatment. Three replicate litterbags per species were buried in each treatment and sampled in the freezing onset, deep freezing and thawing stages and in the early, middle and late snow-free seasons from November 2014 to October 2015. Our results showed that the oak litter decomposed faster than the pine litter, but the magnitude of the effect of snow-depth was greater for the latter because of differences in litter chemical traits. Litter mass loss decreased with decreasing snow-depth in the snow-covered season but increased in the following snow-free season. The short-term effect resulting from the changes in soil environments affected litter decomposition during the snow-covered season, while the long-term effect due to litter chemical traits and the microbial biomass carbon concentration controlled the decomposition in the snow-free season. These results indicated that snow-depth significantly influences the foliar litter decomposition of the two tree species with contrasting initial qualities, but the effects differed between the snow-covered and snow-free seasons. These results deepen our understanding of biogeochemical cycling in cold regions.

Effect of Straw Mulch on Soil Evaporation during Freeze–Thaw Periods

Reducing soil evaporation is important to alleviate water shortages in arid and semi-arid regions. The objective of this work was to reveal the effect of straw mulch on soil evaporation based on field experiments during a freeze–thaw period in Northern China. Four soil surface mulch treatment modes were investigated: Bare soil (BS), 1 cm thick straw mulch with 100% coverage rate (J1), 2 cm thick straw mulch with 100% coverage rate (J2), and 2 cm thick straw mulch with 50% coverage rate (J3). Principal component analysis was used to analyze the major factors influencing soil evaporation in three freeze–thaw stages. The results show that cumulative soil evaporation decreased with increased straw mulch thickness and coverage rate. The effect of straw mulching on soil evaporation was obvious during the stable freezing period, and soil evaporation with straw mulch treatments was reduced by 49.0% to 58.8% compared to BS treatment, while there was little difference for straw mulch treatments in the thawing stage. The relationship between cumulative soil evaporation under different straw mulch modes and time was well fitted by the power function. In the unstable freezing stage, the major factors for all treatments influencing soil evaporation were surface soil temperature and water surface evaporation; in the stable stage, they were solar radiation and relative humidity, and in the thawing stage, they were solar radiation and air temperature. The research results can provide a basis for addressing soil water storage and moisture conservation and restraining ineffective soil evaporation in arid and semi-arid areas.

The freeze/thaw process and the surface energy budget of the seasonally frozen ground in the source region of the Yellow River

The freeze/thaw process and the surface energy budget of the seasonally frozen ground in the source region of the Yellow River were investigated by using observed soil temperature and moisture and the energy flux from May 12, 2014, to May 11, 2015. Compared with the Maduo site, the starting date of the freezing process was later and the freezing depth was shallower at Maqu site. The maximum frozen depth was about 320 cm at Maduo site and 90 cm at Maqu site. The soil temperature of Maqu site was higher than of Maduo site due to lower latitude and altitude. The soil was the driest under the depth of 40 cm and 80 cm at Maduo and Maqu sites, respectively. The diurnal amplitudes of soil temperature of Maduo site were larger than of Maqu site at four freeze/thaw stages; the amplitudes were the largest in the completely thawed stage (9.19 °C and 4.35 °C) and minimal in the freezing stage (1.23 °C and 0.47 °C). The diurnal amplitudes of soil moisture of Maqu site were greater than of Maduo site at all stages. The net radiation Rn’s seasonal change was hardly influenced by the freeze/thaw process. The mean ground heat flux (G0) was negative during the freezing and completely frozen stage and positive during the thawing and completely thawed stage. During the completely thawed and frozen stages, the latent heat flux (LE) and sensible heat flux (H) predominated in the surface energy distribution, respectively. Overall, the variations of fluxes were affected by both the monsoon and freeze/thaw process of the soil layer in seasonally frozen region. The freeze/thaw process had a significant effect on the diurnal change of G0 during the freezing stage. The annual energy closure status of Maduo and Maqu sites was 0.77 and 0.58, respectively. The energy closure status was the highest during the completely thawed stage at Maduo site and during the thawing stage at Maqu site and lowest during the freezing stage among the four stages, due to the snow cover’s impact. Overall, the freeze/thaw process and the high albedo caused by snow cover had effects on the energy closure status.

Trajectory of Topsoil Nitrogen Transformations Along a Thermo‐Erosion Gully on the Tibetan Plateau

AbstractPermafrost thaw, especially thermokarst formation, that is, ground collapse due to thawing of ice‐rich permafrost, is expected to alter soil gross nitrogen (N) transformations, which can regulate plant productivity and ecosystem carbon cycle. However, it remains unclear how thermokarst formation modifies soil N processes in permafrost ecosystems. Here 15N pool dilution techniques were used to evaluate changes in topsoil gross N transformations during various thaw stages (early, middle, and late stages) along a thermo‐erosion gully on the Tibetan Plateau. Structural equation modeling was then conducted to explore the relative importance of biotic and abiotic factors in affecting soil gross N transformations. The results showed that topsoil gross N mineralization (GNM) decreased at the three stages, reflecting declined inorganic N production after permafrost collapse. In contrast, topsoil gross nitrification increased only during the early stage. Additionally, the ratio of microbial N immobilization to GNM was enhanced during the middle and late stages, indicating a stronger microbial N limitation after thermokarst formation. The structural equation modeling analysis revealed that soil moisture played an important role in modulating gross N transformations. For GNM, decreased soil moisture had inhibiting effects via regulating the microbial biomass, microbial community, and enzyme activities along the thaw sequence. For gross nitrification, declined soil moisture exerted facilitating effects directly by improving oxygen availability and indirectly by modulating the abundances of ammonia‐oxidizing archaea and bacteria during the early stage. Overall, these results demonstrated that thermokarst formation altered soil N processes, potentially triggering interactions between ecosystem N and carbon cycles after permafrost thaw.

Large carbon cycle sensitivities to climate across a permafrost thaw gradient in subarctic Sweden

Abstract. Permafrost peatlands store large amounts of carbon potentially vulnerable to decomposition. However, the fate of that carbon in a changing climate remains uncertain in models due to complex interactions among hydrological, biogeochemical, microbial, and plant processes. In this study, we estimated effects of climate forcing biases present in global climate reanalysis products on carbon cycle predictions at a thawing permafrost peatland in subarctic Sweden. The analysis was conducted with a comprehensive biogeochemical model (ecosys) across a permafrost thaw gradient encompassing intact permafrost palsa with an ice core and a shallow active layer, partly thawed bog with a deeper active layer and a variable water table, and fen with a water table close to the surface, each with distinct vegetation and microbiota. Using in situ observations to correct local cold and wet biases found in the Global Soil Wetness Project Phase 3 (GSWP3) climate reanalysis forcing, we demonstrate good model performance by comparing predicted and observed carbon dioxide (CO2) and methane (CH4) exchanges, thaw depth, and water table depth. The simulations driven by the bias-corrected climate suggest that the three peatland types currently accumulate carbon from the atmosphere, although the bog and fen sites can have annual positive radiative forcing impacts due to their higher CH4 emissions. Our simulations indicate that projected precipitation increases could accelerate CH4 emissions from the palsa area, even without further degradation of palsa permafrost. The GSWP3 cold and wet biases for this site significantly alter simulation results and lead to erroneous active layer depth (ALD) and carbon budget estimates. Biases in simulated CO2 and CH4 exchanges from biased climate forcing are as large as those among the thaw stages themselves at a landscape scale across the examined permafrost thaw gradient. Future studies should thus not only focus on changes in carbon budget associated with morphological changes in thawing permafrost, but also recognize the effects of climate forcing uncertainty on carbon cycling.

Potentials and pitfalls of permafrost active layer monitoring using the HVSR method: a case study in Svalbard

Abstract. Time-lapse monitoring of the subsurface using ambient seismic noise is a popular method in environmental seismology. We assess the reliability of the horizontal-to-vertical spectral ratio (HVSR) method for monitoring seasonal permafrost active layer variability in northwest Svalbard. We observe complex HVSR variability between 1 and 50 Hz in the record of a temporary seismic deployment covering frozen and thawed soil conditions between April and August 2016. While strong variations are due to changing noise conditions, mainly affected by wind speed and degrading coupling of instruments during melt season, a seasonal trend is observed at some stations that has most likely a subsurface structural cause. A HVSR peak emerges close to the Nyquist frequency (50 Hz) in beginning of June which is then gradually gliding down, reaching frequencies of about 15–25 Hz in the end of August. This observation is consistent with HVSR forward modeling for a set of structural models that simulate different stages of active layer thawing. Our results reveal a number of potential pitfalls when interpreting HVSRs and suggest a careful analysis of temporal variations since HVSR seasonality is not necessarily related to changes in the subsurface. In addition, we investigate if effects of changing noise sources on HVSRs can be avoided by utilizing a directional, narrowband (4.5 Hz) repeating seismic tremor which is observed at the permanent seismic broadband station in the study area. A significant change of the radial component HVSR shape during summer months is observed for all tremors. We show that a thawed active layer with very low seismic velocities would affect Rayleigh wave ellipticities in the tremor frequency band. We compile a list of recommendations for future experiments, including comments on network layouts suitable for array beamforming and waveform correlation methods that can provide essential information on noise source variability.

Simulation of land surface heat fluxes in permafrost regions on the Qinghai-Tibetan Plateau using CMIP5 models

The variations in land surface heat fluxes affect the ecological environment, hydrological processes and the stability of surface engineering structures in permafrost regions of the Qinghai-Tibetan Plateau (QTP). Based on observation data from a meteorological station in the Tanggula site in 2005, which is located in a permafrost region on the QTP, the performances of seventeen selected the phase 5 of the Coupled Model Intercomparison Project (CMIP5) were evaluated. The results showed that these simulations did not perform well using sensible heat flux, downward shortwave radiation or upward shortwave radiation, and differences exist among the models. The average multimodel ensemble results were similar to the observed land surface heat fluxes. The results revealed that the monthly average latent heat flux and the net radiation were small in December and January and large in May, June and July. The fluctuation in the soil heat flux was well correlated with the net radiation, and the sensible heat flux was negative in January and December in northwest of the Plateau. The latent heat flux was the strongest over the southeastern QTP from May to August, and it decreased over the northwestern QTP. In contrast, the sensible heat flux was the weakest over the southeastern QTP, and it gradually increased and became dominant over the northwestern QTP. The results also indicated that there was a good correlation between the surface heating field intensity and the net radiation, with a correlation coefficient of 0.99; this indicates stronger heating over the eastern QTP than over the western QTP and stronger heating over the southern QTP than over the northern QTP. Furthermore, the Bowen ratio was higher during the freezing and thawing stages than that during the completely thawed stage. This ratio was larger over the central and northeastern QTP and smaller along the northwest edge of the QTP, which was lower (range from −0.81 to 4.86) due to the overestimation of precipitation, a smaller difference between the simulated monthly average surface temperature and the observed air temperature, and a decrease in wind speed when using the CMIP5 models in the permafrost region of the QTP. This research provides a foundation for understanding land surface heat flux characteristics in the permafrost regions on the QTP under climate change.

Impact of amphiphilic compounds on post-hypertonic shock of human erythrocytes

When the cryopreserved erythrocytes are thawed, with the ice melting the extracellular hypertonic medium changes to isotonic one, resulting in post-hypertonic cell lysis development. Under experimental conditions, the post-hypertonic shock of erythrocytes simulates the influence of cryodamage factors, acting at the erythrocyte thawing stage, as well as when the cells, cryopreserved under protection of penetrating cryoprotectant are transferred into bloodstream. Post-hypertonic shock of erythrocytes was carried out by transferring the cells from a hypertonic solution contained 1.65 mol/l NaCl (dehydration medium) into an isotonic one with 0.15 mol/l NaCl (rehydration medium) at 0ºС. The effect of specimens of various classes of amphiphilic compounds (anionic sodium decyl sulfate, non-ionic decyl-β,D-glucopyranoside, and cationic chlorpromazine) on the human erythrocyte sensitivity to post-hypertonic shock, was studied. Amphiphilic substances were supplemented into rehydration medium prior to cell introduction into it. It was shown that under post-hypertonic shock of erythrocytes, all the studied amphiphilic substances, when used in efficient concentrations, manifested a high anti-hemolytic activity (at the level of 70%). A comparative study of the efficiency of amphiphilic substances under post-hypertonic shock of erythrocytes showed differences in size of the plateau (the concentration range of amphiphilic compound, within the limits of which the minimum level of erythrocyte hemolysis was observed). Thus, it was found that for non-ionic decyl-β,D-glucopyranoside the plateau was 3 times more than for anionic sodium decyl sulfate and cationic chlorpromazine. The minimum efficient concentration for sodium decyl sulfate and the maximum one for decyl-β,D-glucopyranoside under post-hypertonic shock of erythrocytes were revealed. It is assumed that the revealed protective effect of amphiphilic compounds under post-hypertonic shock of erythrocytes is associated with their capability to integrate into membrane. This entails an increase in the surface area of the membrane and, therefore, the critical hemolytic volume of cell, which allows it to swell to a larger volume.

Large-scale permafrost degradation as a primary factor in Larix sibirica forest dieback in the Khentii massif, northern Mongolia

The objective of this study is to investigate the potential causes of widespread Larix sibirica Ledeb. mortality observed in the Khentii massif of northern Mongolia. The ratio of deadwood to living trees in affected stands in the Goricho region, the southernmost study site situated close to the Gobi Desert, was as high as 3.6:1. Moisture fluctuations monitored over 2 years using electrical impedance spectrometry revealed that the Goricho study site had higher soil moisture levels than the two less affected sites Barun Bayan and Dzun Bayan. High soil moisture was recorded in an area characterized by highly skeletal soils, ones with more than 35% by volume of rock fragments, and comparatively shallow soil horizons, from valley to mountains. The layer of permafrost influencing hydrogeological processes is much deeper in the Goricho region compared to the undisturbed study sites. Redundancy analysis confirmed a significant number of dead L. sibirica on sites with developed soils. Live forest stands, however damaged, grow in this region on well-drained scree slopes or on rocky bastions. The mass mortality observed for L. sibirica may be directly linked to accelerated permafrost thaw in the area bordered by the Tuul and the Terelj Rivers. Our assumption is that L. sibirica root system necrosis occurred as a result of long-term waterlogging of developed soils with high spatial heterogeneity, normally able to absorb high quantities of groundwater. The areas unaffected were scree fields and rocky bastions characterized by adequate drainage. All of our findings support the primary stages of large-scale permafrost thaw, i.e., correlating increases in soil moisture with increasing permafrost active layer thickness.

A Study of Soil Thermal and Hydraulic Properties and Parameterizations for CLM in the SRYR

AbstractLocated in the northeast part of the Qinghai‐Tibet Plateau, the source region of the Yellow River is a crucial water conservation area. Laboratory tests and observations are used to determine the soil thermal and hydraulic properties in this key area. Furthermore, their physical processes in the Community Land Model (CLM) are revised to better apply to the plateau. The main results are as follows. (1) The thermal conductivity in frozen soil is mainly controlled by the content of air and is positively correlated with subzero temperatures (−5 to −20 °C); however, it slightly increases with increasing temperature (5 to 20 °C) in thawed soil, and obvious layering exists. The influence of temperature can be ignored for dry soil. (2) The thermal conductivity of thawed soil is positively correlated with water content and shows a nonlinear variation. The rate of increase is rapid during the early stage of thawing and then slows. (3) The saturated hydraulic conductivity decreases with depth. Soil matric potential exhibits large fluctuations and is spatially heterogeneous due to the feedback to soil moisture. (4) The simulation differences of soil temperature and moisture are narrowed to approximately 1 °C and by half, respectively, by the revised schemes in CLM4.5. The simulation of energy fluxes and ground temperature also improved, but the latent heat flux performed less well due to the deficiencies in the hydraulic schemes. This study is of great significance for research on land surface processes and the application of the CLM in the Qinghai‐Tibet Plateau.

N2O emission from a temperate forest soil during the freeze-thaw period: A mesocosm study

Nitrous oxide (N2O) is an important greenhouse gas and is involved in the destruction of ozone layer. However, the underlying mechanisms of the high soil N2O emission during the freeze-thaw (FT) period are still unclear. Here, we conducted a mesocosm study with high frequency in situ measurements to explore the responses of soil microbes to the FT cycles and their influences on soil N2O emission. We found the high N2O emission rate during the FT period was mainly due to the release of substrates, the maintenance of high enzyme activities at the freezing stage, and the fast recovery of microbial biomass nitrogen (MBN) and high microbial activities at the thawing stage. Physical isolation of previously produced N2O was an important mechanism for the higher N2O flux at the thawing stage. With increasing numbers of the FT cycles, MBN at the thawing stage remained stable and potential dehydrogenase activities at the thawing stage also remained stable after the first eight FT cycles and only declined during the last two cycles, suggesting the sustainability of the biological mechanisms. Our study suggests that although MBN declined, microbial enzymes could maintain high activities at a few degrees Celsius below zero in this temperate forest soil and produce high N2O fluxes even at the freezing stage, which were trapped under the ice layer and released at the thawing stage, resulting in high soil N2O emission during the FT period.

Effect of Sand Mulches of Different Particle Sizes on Soil Evaporation during the Freeze–Thaw Period

Reducing ineffective evaporation is a feasible means to improve water use efficiency in agriculture, especially in arid and semi-arid areas. A field experiment assessed the impact of sand mulches (1-cm thickness) with a particle size of 0.5–1.5 mm (XS) and 1.5–2.0 mm (CS) on soil evaporation during winter in Northern China. Results showed that CS and XS increased by at least 11.93% and 14.92% compared to bare soil (LD), respectively. However, the sand mulches showed significant distinctions in soil evaporation at different stages. Soil evaporation was the highest in LD, while CS evaporated the least in the unstable freezing stage (P1) and stable freezing stage (P2); in the thawing stage (P3), XS evaporated the most, while LD evaporated the least. Ten factors affecting evaporation were analyzed using the principal component analysis method to elaborate the mechanisms of soil evaporation. Mean soil moisture at depths from 0 to 15 cm was a factor that affected the evaporation of XS and CS in the test. Soil moisture was evaporated by vapor when the frost penetration was dense and the frost impeded the vapor movement. The evaporation rates were steady and weak in this period, and soil moisture had slight impact on soil evaporation, especially XS and CS treatment with higher water content at the surface. The research is significant for preventing evaporation and the improvement of water-use efficiency.

Freeze desalination by the integration of falling film and block freeze-concentration techniques

Block and falling film freeze concentration are two technologies that separate water by freezing, with the potential to desalinate seawater. In this study, the integration of two freeze concentration techniques as an alternative to obtain potable water was analysed. Water with 0.5%–8% NaCl was freeze-concentrated by the falling film technique. The ice from each stage was fractionally thawed to recover the solids retained in the ice. The diluted fractions of the thawing stage were freeze-concentrated using the block technique to increase water purity. Falling film freeze concentration was effective to separate the salt from the solution, even at high salt concentrations. Block freeze concentration was effective to increase the water purity until drinkable water was obtained. A multistage process with the integration of these techniques was proposed to obtain 74% of the amount of the initial solution at 0.05% of salt, and 26% at 13.4% of salt. With this process, a salt removal efficiency of 98.5% was achieved. The energy consumption was analysed. The integration of these techniques results in water that meets the requirements for drinkable water and demonstrates the technical feasibility of the process.

Changes in Methane Flux along a Permafrost Thaw Sequence on the Tibetan Plateau.

Permafrost thaw alters the physical and environmental conditions of soil and may thus cause a positive feedback to climate warming through increased methane emissions. However, the current knowledge of methane emissions following thermokarst development is primarily based on expanding lakes and wetlands, with upland thermokarst being studied less often. In this study, we monitored the methane emissions during the peak growing seasons of two consecutive years along a thaw sequence within a thermo-erosion gully in a Tibetan swamp meadow. Both years had consistent results, with the early and midthaw stages (3 to 12 years since thaw) exhibiting low methane emissions that were similar to those in the undisturbed meadow, while the emissions from the late thaw stage (20 years since thaw) were 3.5 times higher. Our results also showed that the soil water-filled pore space, rather than the soil moisture per se, in combination with the sand content, were the main factors that caused increased methane emissions. These findings differ from the traditional view that upland thermokarst could reduce methane emissions owing to the improvement of drainage conditions, suggesting that upland thermokarst development does not always result in a decrease in methane emissions.