Due to the excess release of hazardous pollutants to the environment, the quest for the synthesis of effective nanomaterials for wastewater treatment is never-ending. Present study reports the polyol synthesis of Ag NWs of ~ 85nm diameter and average length of 4.08µm using PVP and ethylene glycol. The experimental data on the methylene blue dye degradation substantiated the photocatalytic efficiency of Ag NWs (88% degradation in 120min). Furthermore, the Ag NWs exhibited microbial load reducing property in air conditioner condensate water (ACW) within a time period of 60min. Also, the anti-bacterial effect of Ag NWs was estimated using two human pathogenic bacterial strains, namely Staphylococcus aureus and Bacillus cereus. The antibacterial potential of Ag NWs against Staphylococcus aureus and Bacillus cereus was revealed significant with an inhibition zone size of 14 ± 0.1mm and 9 ± 0.1mm, respectively. Hence, the present work validates the potential efficiency of Ag NWs in the degradation of textile dyes and reduction of microbial population.