In infection experiments with clover rot (Sclerotinia trifoliorumErikss.) on 9 pairs of diploid and corresponding autotetraploid families arisen from chimaera plants the tetraploid families proved to be more resistant than their corresponding diploid families. Significant interaction between family pairs and ploidy level was demonstrated and this is an indication that there may be differences between different genotypes with respect to the effect of autotetraploidy on resistance to clover rot in red clover.