The growth responses of 10 Rhizoctonia zeae isolates, obtained from turfgrasses in Florida and Ohio, to four temperatures (20, 25, 30, and 35 °C) and seven fungicides at four concentrations (0, 1, 10 and 100 μg·mL-1 a.i.) were compared. Greenhouse pathogenicity tests were conducted using hybrid bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy]. Optimal temperature for growth for all isolates was 30 °C. Growth of R. zeae isolates from both geographic locations was severely limited (>75%) at 20 °C. All R. zeae isolates were insensitive to the benzimidazole fungicides, benomyl and thiophanate methyl. Their sensitivities to iprodione, mancozeb, and quinotzene fungicides were similar. The Florida isolates were more sensitive to chlorothalonil, and the Ohio isolates to thiram. All isolates were pathogenic to hybrid bermudagrass. Chemical names used: methyl 1-(butylcarbamoly)-2-benzimidazolecarbamate (benomyl); dimethyl 4,4′-O-phenylene bis(3-thioallophanate) (thiophanate methyl); pentachloronitrobenzene (quintozene); 3-(3,5-dichlorophenyl)-N-(1-methylethyl)-2,4-dioxo-1-imidazolidinecarboxamide (iprodione); tetrachloroisophtalonitrile (chlorothalonil); tetramethylthiuram disulfide (thiram); manganese ethylenebisdithiocarbamate (mancozeb).