Tetrabromobisphenol A Research Articles

Environmentally relevant exposure to tetrabromobisphenol A induces reproductive toxicity via regulating glucose-6-phosphate 1-dehydrogenase and sperm activation in Caenorhabditis elegans

Tetrabromobisphenol A (TBBPA), a ubiquitous brominated flame-retardant environmental pollutant, has been reported to cause reproductive toxicity by chronic exposure. However, the acute reproductive risk and mechanisms of TBBPA toxicity to individuals, especially at environmentally relevant levels, remains a topic of debate. In this study, Caenorhabditis elegans was used to investigate the reproductive toxicity of acute exposure to TBBPA at environmentally relevant doses. The reproductive end points (embryonic lethality ratio and brood size), oxidative stress, sperm activation, and molecular docking were evaluated. Results showed that, after 24 h of TBBPA treatment, even at the lowest concentration (1 μg/L), the embryonic lethality ratio of C. elegans increased significantly, from 1.63 % to 3.03 %. Furthermore, TBBPA induced oxidative stress with significantly increased expression of sod-3 in C. elegans, which further raised the level of reproductive toxicity through inhibiting the activation of sperm in nematodes. In addition, molecular docking suggested TBBPA might compete for the glucose-6-phosphate-binding site of glucose-6-phosphate 1-dehydrogenase, resulting in oxidative stress generation. Accordingly, our findings indicate that even acute exposure to environmental concentrations of TBBPA may induce reproductive toxicity through reducing sperm activation in nematodes.

Physiology-informed toxicokinetic model for the zebrafish embryo test developed for bisphenols

Zebrafish embryos (ZFE) is a widely used model organism, employed in various research fields including toxicology to assess e.g., developmental toxicity and endocrine disruption. Variation in effects between chemicals are difficult to compare using nominal dose as toxicokinetic properties may vary. Toxicokinetic (TK) modeling is a means to estimate internal exposure concentration or dose at target and to enable extrapolation between experimental conditions and species, thereby improving hazard assessment of potential pollutants. In this study we advance currently existing TK models for ZFE with physiological ZFE parameters and novel experimental bisphenol data, a class of chemicals with suspected endocrine activity. We developed a five-compartment model consisting of water, plastic, chorion, yolk sack and embryo in which surface area and volume changes as well as the processes of biotransformation and blood circulation influence mass fluxes. For model training and validation, we measured internal concentrations in ZFE exposed individually to BPA, bisphenol AF (BPAF) and Z (BPZ). Bayesian inference was applied for parameter calibration based on the training data set of BPZ. The calibrated TK model predicted internal ZFE concentrations of the majority of external test data within a 5-fold error and half of the data within a 2-fold error for bisphenols A, AF, F, and tetrabromo bisphenol A (TBBPA). We used the developed model to rank the hazard of seven bisphenols based on predicted internal concentrations and measured in vitro estrogenicity. This ranking indicated a higher hazard for BPAF, BPZ, bisphenol B and C (BPB, BPC) than for BPA.

Intrinsic Flame Retardancy and Flexible Solid-Solid Phase Change Materials with Self-Healing and Recyclability.

Conventional polymeric phase change materials (PCMs) have been widely used due to their high heat storage density, small temperature variation, and nontoxicity. However, the high flammability and unrecyclable problems restrict their applications in energy storage devices (ESDs). Although it is facile to introduce a flame retardant into phase change materials to improve fire resistance, the physical blending will deteriorate the mechanical performance and thermal stability of PCMs. Herein, flame-retardant solid-solid PCMs (FRPCMs) with intrinsic flame retardancy, phase change property, self-healing, and recyclability were synthesized by simultaneously integrating tetrabromobisphenol A (TBBPA) and poly(ethylene glycol) (PEG) into polyurethane network skeletons. PEG ingredients acted as phase change materials, and TBBPA not only worked as an efficient flame retardant but also provided dynamic covalent bonds for thermally induced self-healing and recyclability. FRPCMs possess the highest latent heat of 124.7 J/g, high self-healing ability, and high thermal reliability and recyclability. Besides, with the introduction of TBBPA, the limiting oxygen index (LOI) value and char residue significantly increased, the heat release rate (HRR) and total heat release (THR) values decreased, and most of the FRPCMs reached UL94 V-2 rating as well. Hence, the synthesized FRPCMs could expand the application scope of PCMs for thermal energy storage.

TBBPA and lead co-exposure induces grass carp liver cells apoptosis via ROS/JAK2/STAT3 signaling axis

Tetrabromobisphenol A (TBBPA) and lead (Pb) are widely used in industrial field, which poses a serious threat to human and animal health. In particular, a large volume of wastewater containing TBBPA and Pb was discharged into the aquatic environment, causing a seriously negative impact on fish. Currently, whether TBBPA and Pb have a synergistic toxicity on fish remains unclear. In this study, we used the grass carp hepatocytes (L8824 cell line) exposed to either TBBPA or Pb, or both to determine their potential impacts on fish. The results showed that Pb or TBBPA induced oxidative stress and the loss of mitochondrial membrane potential in grass carp hepatocytes. In contrast to the control cells, the levels of JAK2, p-JAK2, STAT3 and p-STAT3 were significantly upregulated after exposure to TBBPA and Pb. Furthermore, the levels of Caspase3, Caspase9 and Bax were all increased while the level of Bcl2 was decreased in hepatocytes exposed to TBBPA or Pb. Results of flow cytometry and AO/EB staining reveled significant increases in the number of apoptotic cells in the TBBPA and Pb group compared to the controls. Notably, cells exposed to both TBBPA and Pb exhibited more severe damage than the single exposure, manifested by a higher number of apoptotic cells in the co-exposure group than the single exposure groups. Nevertheless, N-acetyl-l-cysteine (NAC) treatment could remarkably alleviate oxidative damage and loss of membrane potential in grass carp hepatocytes induced by TBBPA and Pb. Altogether, our study showed that combined exposure of TBBPA and Pb has a synergistic toxicity due to, inducing oxidative stress to activate JAK2/STAT3 signaling pathway, resulting in apoptosis of carp hepatocytes. This study shed a new light on the toxicological mechanism of exposure of TBBPA and Pb and provided a potential treatment of toxicity induced by TBBPA and Pb.

Prussian blue analogues derived magnetic FeCo@GC material as high-performance metallic peroxymonosulfate activators to degrade tetrabromobisphenol A over a wide pH range.

Metal-organic frame (MOF) materials can effectively degrade organic pollutants, whereas the MOF is rapidly hydrolysed in water and has poor stability and low reusability. However, in the current advanced oxidation process (AOP) system, the removal effect of pollutants under alkaline condition is not ideal. In this study, a magnetic composite material derived from MOF was synthesised and used as a new catalyst for rapid degradation of tetrabromobisphenol A (TBBPA). Compared to precarbonisation, FeCo@GC formed a conductive graphite carbon skeleton, retained the complete rhombododecahedron structure, had a larger specific surface area and provided more active sites for peroxymonosulfate (PMS) activation. The target pollutant TBBPA (20 mg/L) was completely degraded within 30 min, and the mineralisation rate reached 40.98% in the FeCo@GC (150 mg/L) and PMS (1 mM) systems, owing to the synergistic interaction between Fe, Co and graphite carbon. The reactive oxygen species (ROS) involved in the reaction were determined to be SO4•-, ·OH, 1O2 and O2•- by electron paramagnetic resonance and free radical scavenging experiments, and the 1O2 played a dominant role. Based on the results of LC-MS analysis results, the main degradation pathways of TBBPA involve three mechanisms: the debromination reaction, hydroxylation and cleavage of the benzene ring. In addition, compared with previous AOP systems, FeCo@GC/PMS overcomes the disadvantage of poor degradation effect of TBPPA under alkaline conditions, has a wide range pH (3-11) application and has the best effect on TBBPA degradation under alkaline conditions. FeCo@GC has an excellent cycle performance, with a removal rate of re-calcined material of 88.52% after five cycles. Therefore, FeCo@GC can be used as a promising and efficient catalyst for removing environmental organic pollutants.

Eight novel brominated flame retardants in indoor and outdoor dust samples from the E-waste recycling industrial park: Implications for human exposure

As alternatives for legacy brominated flame retardants, novel brominated flame retardants (NBFRs) have a wide array of applications in the electronic and electrical fields. The shift of recycling modes of electronic and electrical waste (e-waste) from informal recycling family workshop to formal recycling facilities might come with the change the chemical landscape emitted including NBFRs, however, little information is known about this topic. This study investigated the occurrence characteristics, distribution, and exposure profiles of eight common NBFRs and their derivatives in an e-waste recycling industrial park in central China and illustrated the differences in various functional zones in the recycling park. The highest level of ΣNBFRs in dust samples was found in e-waste storage area at median concentration of 27,400 ng/g, followed by e-waste dismantling workshops (23,300 ng/g), workshop outdoor area (7770 ng/g), and residential area outdoor (536 ng/g). In the e-waste dismantling associated dust samples, tetrabromobisphenol A bis(2,3-dibromopropyl ether) (TBBPA-BDBPE), tetrabromobisphenol A (TBBPA) and 2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine (TTBP-TAZ) were the predominant components. This paper presented the first evidence regarding the occurrence characteristic and distribution of tetrabromobisphenol S (TBBPS), tetrabromobisphenol A bismethyl ether (TBBPA-BME) and tetrabromobisphenol S bis(2,3-dibromopropyl ether) (TBBPS-BDBPE) in the e-waste associated dust samples. By comparing with previous studies performed in China, this paper also noticed the significant decrease of TBBPA concentrations in the dust probably due to the shift of e-wastes sources and recycling modes. We further assessed the risk of occupational workers exposure to NBFRs. The median EDI (estimated daily intake) value of ΣNBFRs among e-waste dismantling workers was 9.71 ng/kg BW/d with the maximum EDI value being 19.6 ng/kg BW/d, hundreds of times higher than those exposed by general population. The study raises great concern for the health risk of occupational exposure to NBFRs in the e-waste recycling industrial park.

Efficient removal of tetrabromobisphenol A through persulfate activation by α-MnO2 nanofiber coated with graphene oxide

The development of efficient and environmentally friendly catalysts for activating persulfate is the focus of advanced oxidation treatment of organic contaminants. Manganese dioxide (MnO2), which has a multivalent nature and is a low-toxic natural stock, has the ability to immediately oxidize pollutants. In order to further improve its catalytic performance, the α-MnO2 nanofiber coated with graphene oxide (α-MnO2@GO) was prepared by one-step hydrothermal method, and worked as a peroxmonsulfate (PMS) activator for efficient degradation of tetrabromobisphenol A (TBBPA). The structure characterization results indicate that α-MnO2 nanofibers are successfully encapsulated by graphene and assembled into a three-dimensional layered structure. The factors affecting the catalytic degradation of TBBPA by this catalyst are investigated in detail. Under optimal conditions, the highest removal ratio of TBBPA could reach 100% after treatment of 20 min by α-MnO2@GO/PMS system, and a good mineralization efficiency of up to 79.50% is obtained. Electron paramagnetic resonance detection combined with quenching experiments showed that sulfate radical and hydroxyl radical played a role in the degradation of TBBPA. Based on X-ray photoelectron spectroscopy and free radical scavenging experiments, the peroxmonsulfate activation mechanism by α-MnO2@GO was proposed. Finally, based on the discovered intermediates, two potential TBBPA degradation routes using this catalyst were suggested. This study not only provides a new strategy for improving the catalytic activity of MnO2 but also sheds new insight into the application of eco-friendly natural stock for environmental remediation.

Efficient degradation of tetrabromobisphenol A using peroxymonosulfate oxidation activated by a novel nano-CuFe2O4@coconut shell biochar catalyst

In this study, a novel bimetallic complexation–curing nucleation–anaerobic calcination method was developed to synthesize a nano-CuFe2O4@coconut shell biochar (CuFe2O4@CSBC) catalyst to activate peroxymonosulfate for degradation of tetrabromobisphenol A (TBBPA). The reaction processes of the TBBPA on CuFe2O4@CSBC have been investigated using in situ characterization and metal leaching. The effects of initial reaction conditions and degradation mechanism were investigated. Greater than 99% degradation of TBBPA at 10 mg L−1 was achieved in 30 min under the condition of pH 11, a total organic carbon removal rate of up to 70.67% was achieved and the degradation efficiency was 90% after 5 cycles of CuFe2O4@CSBC use. The degradation was in a second-order reaction at a constant of 0.797 M−1 min−1 (R2 = 0.993). The degradation was attributed to the main active species (SO4·−≈·OH < 1O2), and the surface active site of CuFe2O4@CSBC was the key role. The degradation process involved three main degradation pathways. Path A: ·OH attacked the C–Br bonds (TBBPA→TriBBPA→DBBPA→MBBPA→BPA); Path B: Hydroxylation and decarboxylation; Path C: Dehydrocoupling of TBBPA. What's more, the practical application of the system was very positive, achieved >77% degradation in sewage and industrial wastewater.

Novel Electrochemical Sensor Based on MnO2 Nanowire Modified Carbon Paper Electrode for Sensitive Determination of Tetrabromobisphenol A

In this paper, a MnO2 nanowire (MnO2-NW) modified carbon paper electrode (CP) was developed as a novel electrochemical sensor for the sensitive determination of tetrabromobisphenol A (TBBPA). The MnO2 nanowire was prepared by a hydrothermal synthesis method, and the morphology and structure of MnO2 were characterized using scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The electrochemical performance of TBBPA on MnO2-NW/CP was investigated by cyclic voltammetry, and the result confirmed that MnO2-NW/CP exhibited excellent sensitivity for the determination of TBBPA due to the high specific surface area and good electrical conductivity of the nanowire-like MnO2. Moreover, the important electrochemical factors such as pH value, incubation time and modified material proportion were systematically studied to improve the determination sensitivity. The interferences from similar structure compounds on TBBPA have also been investigated. Under the optimal conditions, MnO2-NW/CP displayed a linear range of 70~500 nM for TBBPA with a detection limit of 3.1 nM. This was superior to some electrochemical methods in reference. The work presents a novel and simple method for the determination of TBBPA.

Halogenated bisphenol A derivatives potently inhibit human, rat, and mouse gonadal 3β-hydroxysteroid dehydrogenases: Structure-activity relationship and in silico molecular docking analysis

Bisphenol A (BPA) is a widely used plastic material, and halogenated BPA derivatives are formed either by synthesis or environmental processes. However, the effect of halogenated bisphenols on steroidogenesis remains unclear. The aim of this study was to compare inhibition of 6 BPA derivatives on gonadal 3β-hydroxysteroid dehydrogenases (3β-HSDs) in three species (human, rat, and mouse). The inhibition on human 3β-HSD2 was tetrabromo BPA (TBBPA, IC50, 1.01 μM)>trichloro BPA (TrCBPA, 3.95 μM)>tetrachloro BPA (TCBPA, 4.14 μM)>monochloro BPA (MCBPA, 4.74 μM)>others with TrCBPA of competitive, TBBPA of noncompetitive and MCBPA/TCBPA of mixed inhibition. The inhibition on rat 3β-HSD1 was TCBPA (1.68 μM)>TrCBPA (1.72 μM)>MCBPA (2.80 μM)>BPA>others with mixed inhibition. The inhibition on mouse 3β-HSD6 was TrCBPA (1.59 μM) >MCBPA (3.36 μM)>TCBPA (3.72 μM)>others with mixed inhibition. Molecular docking analysis showed that TBBPA, TrCBPA, and TCBPA bind to steroid active sites, contacting with catalytic residue Tyr154 of human 3β-HSD2. MCBPA, TrCBPA, and TCBPA bind to steroid active site of rat 3β-HSD1. MCBPA and TrCBPA bind to active site of mouse 3β-HSD6. Regression of lowest binding energy values with Ki values revealed a significant negative linear regression (P < 0.05). In conclusion, halogenated BPA derivatives are more potent inhibitors of three 3β-HSDs than BPA and there is structure-dependent inhibition. SynopsisChlorinated bisphenol derivatives after water chlorination process and other halogenated bisphenols effectively inhibit human and rat 3β-HSD activity, thereby leading to steroid hormone deficiency.

TBBPA causes apoptosis in grass carp hepatocytes involving destroyed ER-mitochondrial function

Tetrabromobisphenol A (TBBPA) is the most-produced brominated flame retardant, which can be found in various industrial and household products. Studies have shown that TBBPA has hepatotoxicity, and could pose a risk to aquatic animals. The endoplasmic reticulum (ER) and mitochondria are two important organelles that are highly dynamic in cells, the homeostasis and orchestrated interactions of which are crucial to maintaining cellular function. The aim of this study was to explore the involvement of ER-mitochondria crosstalk in TBBPA-induced toxicity in aquatic animals' hepatocytes. Herein, we exposed grass carp hepatocytes (L8824 cells) to different concentrations of TBBPA. Our experimental results suggested that TBBPA exposure suppressed cell viability and caused apoptosis of L8824 cells. TBBPA treatment upregulated expressions of ER stress markers, increased reactive oxygen species (ROS) and mitochondrial Ca2+ levels, and reduced mitochondrial membrane potential (MMP) in L8824 cells. However, the pretreatment of 2-aminoethoxydiphenyl borate (2-APB) could alleviate TBBPA-induced cell apoptosis, ER stress, and mitochondrial dysfunction. Additionally, 2-APB pretreat relieved ER-mitochondrial contact and the expression of ER-mitochondrial function-related genes induced by high-dose TBBPA. Taken together, these results indicated that TBBPA caused grass carp hepatocyte apoptosis by destroying ER-mitochondrial crosstalk.

Toxicological evaluation of TBBPA by common carp (Cyprinus carpio) about the in vivo/vitro disturbance of the AHR pathway

Tetrabromobisphenol A (TBBPA) is a widely used plastic additive with high bioaccumulation potential and toxicity on both humans and wildlife. Currently, research on its ecotoxicity and the underlying mechanism is limited. Using common carp (Cyprinus carpio), we evaluated the toxicity of TBBPA, especially focusing on its alteration of a key metabolism-related pathway aryl hydrocarbon receptor (AHR), using in vivo/vitro assays and in silico simulation. The 96 h LC50 of TBBPA of common carp was 4.2 mg/L and belonged to the acute toxic level II. The bioaccumulation potential of TBBPA follows the role of liver > gill > brain and varies between 3- and 14-day exposure. On the AHR pathway respect, as expected, the metabolism-related cyp1a1 and cyp1b1 were upregulated in the liver and brain. Ahr2, the receptor, was also upregulated in the brain under TBBPA exposure. The alteration of gene expression was tissue-specific while the difference between 3- or 14-day exposure was minor. AHR inhibition assay indicated the 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD)-induced AHR transactivation can be inhibited by TBBPA suggesting it is not a potent agonist but a competitive antagonist. In silico analysis indicated TBBPA can be successfully docked into the binding cavity with similar poses but still have AHR-form-specific interactions. Molecular dynamics simulation proved TBBPA can be more flexible than the coplanar ligand TCDD, especially in ccaAHR1b with greater root-mean-square deviation (RMSD), of which TCDD-induced transactivation seemed not to be blocked by TBBPA. This research increased the understanding of TBBPA toxicity and alteration of the AHR pathway, and pointed out the need to perform additional toxicology evaluation of emerging contaminants, especially on non-model species.

Impacts of Halogen Substitutions on Bisphenol A Compounds Interaction with Human Serum Albumin: Exploring from Spectroscopic Techniques and Computer Simulations.

Bisphenol A (BPA) is an endocrine-disrupting compound, and the binding mechanism of BPA with carrier proteins has drawn widespread attention. Halogen substitutions can significantly impact the properties of BPA, resulting in various effects for human health. Here, we selected tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) to investigate the interaction between different halogen-substituted BPAs and human serum albumin (HSA). TBBPA/TCBPA spontaneously occupied site I and formed stable binary complexes with HSA. Compared to TCBPA, TBBPA has higher binding affinity to HSA. The effect of different halogen substituents on the negatively charged surface area of BPA was an important reason for the higher binding affinity of TBBPA to HSA compared to TCBPA. Hydrogen bonds and van der Waals forces were crucial in the TCBPA-HSA complex, while the main driving factor for the formation of the TBBPA-HSA complex was hydrophobic interactions. Moreover, the presence of TBBPA/TCBPA changed the secondary structure of HSA. Amino acid residues such as Lys199, Lys195, Phe211, Arg218, His242, Leu481, and Trp214 were found to play crucial roles in the binding process between BPA compounds and HSA. Furthermore, the presence of halogen substituents facilitated the binding of BPA compounds with HSA.

Tetrabromobisphenol a and its alternative tetrachlorobisphenol a induce oxidative stress, lipometabolism disturbance, and autophagy in the liver of male Pelophylax nigromaculatus

Tetrabromobisphenol A (TBBPA) and tetrachlorobisphenol A (TCBPA) have been widely used as flame retardants. However, their potential health risks to organisms have raised concerns, particularly for liver toxicity. Present study aimed to explore the toxic effects of TCBPA and TBBPA on black-spotted frogs (Pelophylax nigromaculatus) liver oxidative stress, autophagy, and lipid accumulation. After exposure to 0.001, 0.01, 0.1, and 1 mg/L TBBPA and TCBPA for 14 days, the content of cholesterol and triglyceride were significantly elevated. In addition, the malondialdehyde level rose greatly in dose dependent. However, the glutathione level declined in high TBBPA groups (0.01 and 0.1 mg/L). Furthermore, expressions of Beclin1, Atg5, and Atg7 were significantly increased, while p62 was markedly declined, respectively. Results obstained suggested that TBBPA and TCBPA exposure induced liver toxicity in black-spotted frog. This study provided insights into the toxicity mechanism of bisphenol flame retardants in amphibians and will aid in the ecological risk assessment of flame retardants.

Heterogeneous catalytic reactions of in-situ generated bromide ions via hydrodehalogenation of tetrabromobisphenol A in advanced oxidation processes over palladium nanoparticles

Herein, we report a novel and efficient approach for degrading tetrabromobisphenol A (TBBPA) via catalytic hydrodehalogenation (HDH) and advanced oxidation processes (AOP). Using heterogeneous catalytic HDH of TBBPA over palladium nanoparticles (Pd NPs) achieves the in-situ generation of bromide ions (Br-), which effectively accelerate and reinforce the degradation of debromination intermediates during peroxymonosulfate-based AOP. The conversion of TBBPA to bisphenol A (BPA) by the HDH process reaches up to 99.9% debromination efficiency in 1 h under near-neutral condition, along with the removal of 99.4% BPA in 15 min during the AOP. Further studies show that free bromine and 1O2 produced by in-situ generated Br- reacting with peroxymonosulfate are the most effective reactive species for promoting the BPA degradation. Sustainability footprint analysis reveals considerable stability and high sustainable footprint of the integrated process during long-term operation. This work provides a promising strategy to elimination of bromine organic pollutants in water treatment.

Alkali-thermal activated persulfate treatment of tetrabromobisphenol A in soil: Parameter optimization, mechanism, degradation pathway and toxicity evaluation

The continued accumulation of halogenated organic pollutants in soil posed a potential threat to ecosystems and human health. In this study, tetrabromobisphenol A (TBBPA) was used as a typical representative of halogenated organic pollutants in soil, for alkali-thermal activated persulfate (PS) treatment. The results of response surface methodology (RSM) showed a optimal debromination efficiency of TBBPA was 88.99 % under the optimum reaction conditions. Quenching experiments and electron paramagnetic resonance (EPR) confirmed that SO4−•, HO•, O2−• and 1O2 existed simultaneously in the oxidation process. SO4−• played a major role in the initial stage of the reaction, and O2−• played a major role in the the last stage. Based on density functional theory (DFT) and intermediate products, two degradation pathways were proposed, including debromination reaction and β bond scission. Moreover, the basic physical and chemical properties of the soil were affected to a certain extent, while the soil surface structure, elements and functional group composition rarely changed. In addition, the T.E.S.T. analysis and biotoxicity tests proved that alkali-thermal activated PS can effectively reduce the toxicity of TBBPA-contaminated soil, which is conducive to the subsequent safe secondary utilization of soil.

Brominated flame retardants in surface sediment from Western Guangdong, South China: Occurrence, distribution and toxicity in Caenorhabditis elegans

Sediment is the ultimate sink of environmental pollutants. A total of 128 surface sediment samples were collected from 8 rivers and 3 reservoirs in Maoming City, Guangdong Province. This study assessed the content and distribution of brominated flame retardants in sediments. The acute toxicity effects of tetrabromobisphenol A (TBBPA) and hexabromocyclododecane (HBCDs) in sediments were evaluated using Caenorhabditis elegans as model organisms. The concentration of TBBPA in sediments ranged from not detected (ND) to 12.59 µg/kg and was mainly distributed in the central area, which was affected by the emission of TBBPA from residential and factory. The concentration of HBCDs ranged from ND to 6.31 µg/kg, and the diastereoisomer distribution was consistent, showing a trend close to the South China Sea. The composition pattern of HBCDs in the surface sediments from rivers were 41.73%–62.33%, 7.89%–25.54%, and 18.76%–40.65% for α-, β-, and γ-HBCD, respectively, and in the sediments from reservoirs were 26.15%–45.52%, 7.44%–19.23%, and 47.04%–61.89% for α-, β-, and γ-HBCD, respectively. When the sum of concentrations of TBBPA and HBCD in sediments were above high levels, reactive oxygen species in nematodes significantly increased, resulting in an oxidative stress response. Intestinal permeability was also enhanced, causing intestinal damage. In addition, in terms of this study, TBBPA had a greater impact on biotoxicity compared to HBCDs, and more attention should be paid to the toxic effects of the river ecosystem organisms in Maoming City, Guangdong Province. This study can complement the pollution database in the study area and provide basic data for pollution control.

Unveiling the occurrence, distribution, removal, and environmental impacts of 65 emerging contaminants in neglected fresh leachate from municipal solid waste incineration plants

Emerging contaminants (ECs) are commonly found in environmental media. Yet leachate from municipal solid waste incineration plants (MSWIPs), which can serve as a reservoir for various contaminants, including ECs, has received little investigation. To address this gap, 65 ECs were analyzed in the fresh leachate and biological effluent from three major MSWIPs in Shanghai. Results indicated that over half (56%) of the 65 ECs were detected in fresh leachate. Different ECs would be removed to varying degrees after biological treatment, including polycyclic aromatic hydrocarbons (PAHs) (65%), polybrominated diphenyl ethers (PBDEs) (51%), phthalate esters (PAEs) (36%), and organophosphorus pesticides (OPPs) (34%). Notably, for tetrabromobisphenol A (TBBPA), a PBDE substitute, only 2% was removed after biological treatment, while polychlorinated biphenyls (PCBs) were effectively removed at 83%. Water solubility and the octanol-water partition coefficient are key factors influencing the distribution and removal of ECs in leachate. the effluent will still contain refractory ECs even after the biological treatment. These residual ECs discharged to sewers can impact wastewater treatment plants or contaminate surface water and groundwater. These findings provide insights into the leachate contamination by ECs, their environmental fate, factors affecting their behavior, and potential environmental impacts.

Two halogenated flame retardants and cadmium in the soil-rice system: sorption, root uptake, and translocation.

The migration and transformation of Tetrabromobisphenol A (TBBPA), DechloranePlus (DP), and cadmium in soil-rice system was investigated, and the influence on the quality of two varieties of rice was studied. The degradation half-lives of TBBPA, BBPAs, syn-DP, and anti-DP were 23.18 ~ 26.36days, 30.14 ~ 36.10days, 72.96-81.55days, and 169.06-198.04days in the soil. TBBPA was gradually degraded to tri-BBPA, di-BBPA, mono-BBPA, and bisphenol A by the debromination. TBBPA and its bromide metabolites could be bioaccumulated in different tissues of rice; mono-BBPA and bisphenol A was easy to accumulate in the stems, and bisphenol A was easy to bioaccumulate in the grain. Comparing with single and compound pollution, there was no significant difference in bioaccumulation factors of two rice species. The grain of NO7 had stronger bioaccumulation ability to mono-BBPA and BPA than NO1, and there is no significant difference in TBBPA. Residual level of DP in the rice: roots > stems > grain; there was no significant difference in bioaccumulation of two varieties of rice. Cadmium was easily bioaccumulated in the roots of rice and translocated to the rice stems and grains. NO7 rice had stronger bioaccumulation and transport capacity than NO1. The effects of the three pollutants on the quality of two varieties of rice varied significantly; cadmium had the greatest effect on the iodine blue value (BV) and amylase activity of the grain. This study proved that selecting rice varieties with low bioaccumulation to polluters can effectively reduce the risk of the food chain harming human health.

Effect of tetrabromobisphenol A (TBBPA) on early implantation using the three-dimensional spheroid model with human endometrial cell line, Ishikawa

BackgroundTetrabromobisphenol A (TBBPA) can be characterized as an endocrine-disrupting chemical (EDCs). It has been widely used as a brominated flame retardant in industrial products. EDCs have effects on female reproduction leading to issues, such as infertility, hormone imbalance, and endometriosis. In Korea, the problems of infertility and decreasing birth rate are of significant concern. Exposure to EDCs might have a harmful effect on female fertility by mediating a decrease endometrial receptivity. This study aimed to investigate the effects of TBBPA on infertility, particularly on early implantation events in the uterine endometrium. Human endometrial adenocarcinoma and trophoblastic cell lines were used in this study. The cytotoxicity of TBBPA on Ishikawa cells and Jeg-3 cells was measured using the Cell Counting Kit-8 assay. The mRNA expression was analyzed by reverse transcription-quantitative polymerase chain reaction, and protein levels were measured by western blotting. The attachment rate was analyzed using an attachment assay, and the outgrowth area was measured using an outgrowth assay.ResultsThe mRNA expression of interleukin (IL)-6, IL-1β, tumor necrosis factor-α, and leukemia inhibitory factor was significantly increased upon treatment of Ishikawa cells by TBBPA. Moreover, the outgrowth area in the TBBPA group was significantly decreased compared to that in the control. In contrast, TBBPA had a minor effect on protein levels and attachment rates.ConclusionsIn this study, TBBPA induced an inflammatory milieu in mRNA expression. An increase in inflammation-related cytokines in the endometrium can disrupt embryo implantation. TBBPA disrupted the outgrowth of spheroids in the endometrium; however, the protein levels and attachment rate were comparable to those in the control group. The effect of TBBPA on implantation events should be elucidated further.