In this paper, some of the basic information on Ground-Penetrating Radar (GPR), its applications (especially in the field of civil engineering) and limitations are presented. As a non-destructive technique, GPR is a powerful tool for the investigation of structures and structural members, roads, geological layers, archaeological sites and many more. The technology is based on electromagnetic radiation in the UHF/VHF range (10 MHz to 3 GHz). The choice of the frequency depends on the intended use, depth and size of the target and medium where the target is located. Joined with other testing methods (ultrasound method, dynamic methods with forced or ambient vibrations, electrical conductivity testing, etc.), GPR can provide a deep insight into the investigated object. However, like many other non-destructive methods, the choice of input parameters may affect the results. In this regard, a case study presented in this paper demonstrates not only different applications of GPR in civil engineering but also the determination (calibration) of one of those input parameters: the dielectric constant of glued laminated timber. The challenge here was not only to investigate the influence of the direction of measurements with regards to the direction of the fibers but also to acknowledge the contribution of the test antenna used during testing and dielectric constant calibration.