Test System Research Articles

Novel real-time PCR methods for bovine haplotypes HH3, HH6, HH7 diagnosis

Dairy cattle breeding is aimed at improving the productivity, mainly through the use of a limited number of breeding bulls. As a result, an increase in inbreeding is observed causing accumulation of heterozygotes-carriers of recessive lethal mutations. A rise in the number of carriers reduces the profitability of dairy farms, since the frequency of embryonic and post-embryonic mortality increases, and the fertility of cows decreases. This paper presents the results of the development of test systems for rapid and inexpensive diagnostics of genetically determined cattle diseases that are significant for animal husbandry, namely for Holstein haplotypes 3, 6 and 7. The diagnostic technology is real-time PCR using TaqMan probes. Carriers of the Holstein haplotype 3 were not found in any of the studied populations. The carrier frequencies for HH6 and HH7 were 0.95 and 1.92%, respectively. Carrier frequencies are consistent with the results of studies worldwide, however, it is worth noting that only few large-scale screening studies have been carried out, since causative loci have been mapped relatively recently.

Numerical and Experimental Study on Dummy Blade with Underplatform Damper

To confirm the variation in damping ratio offered by dry friction dampers against structural vibration stress, this study developed a blade vibration response test system for capturing damping characteristic curves through both frequency sweep excitation and damping-freevibration methods. The damping-free vibration method demonstrates high efficiency, allowing for the acquisition of a complete damping ratio characteristic curve in a single experiment. Experimental findings indicate that the two contact surfaces on the triangular prism damper produce distinct damping effects, closely aligning with the predicted damping characteristic curves. The peak damping ratio was found to be independent of the centrifugal load of the damper; dampers with varying contact areas produce approximately similar damping characteristics; and the damping effect shows a positive correlation with the root extension length.

Numerical and Experimental Study on Dummy Blade with Underplatform Damper

To confirm the variation in damping ratio offered by dry friction dampers against structural vibration stress, this study developed a blade vibration response test system for capturing damping characteristic curves through both frequency sweep excitation and damping-freevibration methods. The damping-free vibration method demonstrates high efficiency, allowing for the acquisition of a complete damping ratio characteristic curve in a single experiment. Experimental findings indicate that the two contact surfaces on the triangular prism damper produce distinct damping effects, closely aligning with the predicted damping characteristic curves. The peak damping ratio was found to be independent of the centrifugal load of the damper; dampers with varying contact areas produce approximately similar damping characteristics; and the damping effect shows a positive correlation with the root extension length.

Qualitative and quantitative protease activity tests based on protein degradation in three-dimensional structures

The pattern of the activity of proteases is related to distinct physiological states of living organisms. Often activity changes of a certain protease can be assigned to a specific disease. Hence, they are useful biomarkers and a simple and fast determination method of their activity could be a valuable tool for the efficient monitoring of numerous diseases. Here, two different methods for the qualitative and quantitative determination of protease activity are demonstrated using the model system of proteinase K. The first test system is based on a protein-modified and colored 3D silica structure that changes color when exposed to the enzyme. This method has also been used for the detection of matrix metallo-protease 2 (MMP2) with gelatine as protease substrate on the plates. The second detection system uses the decrease in the voltammetric signal of a cytochrome c/DNA multilayer electrode after incubation with a protease to quantitatively determine its proteolytic activity. While activities down to 0.15 U/ml can be detected with the first method, the second one provides detection limits of about 0.03U/ml (for proteinase K.) The functionality of both systems can be demonstrated and ways for further enhancement of sensitivity have been elucidated.

Enzyme immunoassay system for serological diagnosis of hemorrhagic fever with renal syndrome based on inactivated purified Puumala virus (Hantaviridae: Orthohantavirus)

Hemorrhagic fever with renal syndrome (HFRS) is the most common zoonotic human viral disease in the Russian Federation. More than 98% of the HFRS cases are caused by Puumala orthohantavirus (PUU). Effective serological tests are required for laboratory diagnosis of HFRS. Construction of an enzyme immunoassay (ELISA) test system for detection of specific antibodies using standard antigen in the form of highly purified inactivated PUU virus as immunosorbent. Preparation of PUU virus antigen, designing the ELISA for detection of specific antibodies, developing parameters of the ELISA system, parallel titration of HFRS patients sera by fluorescent antibody technique (FAT) and the new ELISA. For the first time, ELISA based on purified inactivated PUU virus as standard antigen directly absorbed onto immunoplate was developed. Parallel titration of 50 samples from HFRS patients blood sera using FAT and the developed ELISA showed high sensitivity and specificity of this ELISA, with 100% concordance of testing results and significant level of correlation between the titers of specific antibodies in the two assays. The ELISA based on purified inactivated PUU virus as an immunosorbent can be effectively used for HFRS serological diagnosis and for mass seroepidemiological studies.

Fault classification and localization of multi-machine-based ieee benchmark test case power transmission lines using optimizable weighted extreme learning machine

Accurate fault diagnosis in transmission lines is crucial to ensure the reliability and stability of power grids. Conventional approaches often rely on expert knowledge or complex feature extraction methods, which are subjective and time-consuming. Additionally, many existing approaches use separate sub-algorithms for fault classification and localization. These are operating independently and sequentially. This research work proposes an innovative method for fault classification and localization in transmission lines using phasor measurement unit (PMU) data. The proposed method employs a Weighted Extreme Learning Machine (WELM) algorithm, which uses the variable data distribution across different fault classes through a weighted approach. The PMU data is generated by simulation using an IEEE 9-bus test system in the MATLAB simulation environment. The Maximal Overlap Discrete Wavelet Transform-based feature extraction technique is applied to derive input feature data to facilitate fault classification and localization. The WELM classifier is also optimized using the Grey Wolf Optimization (GWO) algorithm. The resulting GWO-optimized WELM (GWO-WELM) model, when trained on PMU data, achieves a remarkable fault classification accuracy of 99.83 % and fault localization accuracy of 95.48 %, respectively. These results demonstrate that the GWO-WELM model outperforms commonly used classifiers. Moreover, the proposed model shows robustness by accurately classifying the noisy data with a signal-to-noise ratio (SNR) of 10 dB (achieving 91.5 % accuracy in classification and 88.1 % accuracy in localization, respectively).

P-414 Evaluation of small non-coding RNAs in uterine fluid in the diagnosis of endometrial receptivity in assisted reproductive technology programs

Abstract Study question To analyze endometrial receptivity in patients undergoing frozen-thawed embryo transfer (FET) based on the expression of small non-coding RNAs (sncRNAs): microRNAs and piwiRNAs. Summary answer According to our studies, the expression of sncRNAs in uterine aspirate may become an additional non-invasive and effective way to determine endometrial receptivity. What is known already Today, infertility remains an important medical and social problem. According to statistics, the effectiveness of ART programs remains at the level of 30-40%. Violation of the implantation mechanism is associated with a shift in the “implantation window” period and endometrial receptivity. Therefore, the search for an ideal marker of endometrial receptivity is a priority. Studying the expression of sncRNAs in endometrial secretions or uterine aspirate may become a potential marker of endometrial receptivity. Study design, size, duration The study included 102 infertile couples with women aged from 23 to 40 years. The patients were divided into two groups depending on the preparation of the endometrium for the FET: group 1 - 42 patients with natural cycle (NC); group 2 - 60 patients with hormone replacement therapy (HRT). SncRNAs were assessed in uterine aspiration on the day of FET. Participants/materials, setting, methods Uterine fluid was carried out in a volume of 5 to 50 μl with noninvasive technique. Next, the material was sent to the laboratory of applied transcriptomics for identification of sncRNAs. Quantitative assessment of sncRNAs in the endometrial secretion on the day of FET was carried out by deep sequencing on the NextSeq 500/550 platform (Illumina). Main results and the role of chance Logistic regression models were constructed for predicting pregnancy and endometrial receptivity based on the expression profiles of microRNAs and piwiRNAs in uterine aspirate, which are with 95% sensitivity and 74% specificity in the case of a combination of miR-34c-5p, miR-363–3p, miR-1180–3p, miR-361–3p, miR-183–5p (AUC = 0.91, p < 0.001) or with 74% sensitivity and 87% specificity in the case of a combination of molecules piR_004152, piR_020541, piR_001318, piR_014923, piR_017716 (AUC = 0.81, p < 0.001) determine the readiness of the endometrium for embryo implantation in the FET. In the NC and HRT groups, statistically significant differences were established between patients who became pregnant and patients with a negative result of the ART program in terms of baseline progesterone levels (p < 0.001). In the HRT group, positive correlations were revealed between endometrial thickness on the day of embryo transfer and the baseline level of progesterone (p = 0.04, r = 0.28). Limitations, reasons for caution The study was limited in sample size of patients and requires further randomized studies. Wider implications of the findings For the first time, a test system has been developed for assessing endometrial receptivity based on the expression of sncRNAs in patients both in the NC and in the HRT during the FET. It was found that increased initial progesterone levels correlate with positive outcomes of ART programs. Trial registration number not applicable

Voltage stability analysis considering dynamic interaction for power systems integrated with multi-PMSGs

This paper presents the dynamic voltage stability index based on the voltage oscillation loop for power systems integrated with multiple permanent magnet synchronous generators (PMSGs) to assess the dynamic interaction on voltage stability. First, the model of power systems integrated with multi-PMSGs using a Q–V sensitivity matrix as feedback to connect all PMSGs is established. The transfer function model between voltage and reactive power is derived, and the voltage oscillation loop can be derived, which exists in each PMSG. Next, the dynamic voltage stability index is theoretically derived and given, the impact of dynamic interaction among multi-PMSGs on voltage stability can be quantified, and the source of voltage oscillation can be identified and determined. Then, the voltage stability is recovered by shedding the trouble-making PMSG. The parameters of PMSGs could be tuned with the guidance of the dynamic voltage stability index to reduce the risk of voltage oscillation. Finally, the example of a test system with PMSGs is used to validate the correctness of the dynamic stability index.

Mancozeb induces cytogenotoxicity in meristematic cells of Allium cepa L

Mancozeb is a fungicide of the dithiocarbamate functional group, and it is widely used in agriculture to control various fungal diseases. Thus, studies detailing its toxicological characteristics are necessary, as the population may be exposed through the consumption of food or water contaminated with mancozeb. The aim of this study was to evaluate the cytotoxic, genotoxic, and mutagenic potentials of this dithiocarbamate using the Allium cepa L. test system as well as its cytotoxicity in erythrocytes of female rats (Rattus norvegicus). The meristematic roots of A. cepa bulbs were exposed to various concentrations of mancozeb (62.5, 125, 250, and 500 mg/L) for 24, 48, and 72 h to determine cytotoxicity by evaluating the mitotic index (MI), chromosomal aberrations (CA), and nuclear anomalies (NA) for genotoxicity analysis and micronuclei (MN) for mutagenicity analysis. Distilled water and copper sulfate (0.0006 mg/L) were used as the negative control (NC) and positive control (PC), respectively. The MI and the sum of CA and NA of all the mancozeb concentrations showed a significant difference (p ≤ 0.05) in relation to the NC, indicating possible cytotoxicity and genotoxicity induced by mancozeb. Additionally, MN significantly increased with mancozeb concentration from 250 mg/L to 500 mg/L in 24 h when compared to NC. In another study model, mancozeb showed to be cytolytic at concentrations starting from 125 mg/L. Therefore, these results indicate that mancozeb causes cytogenetic alterations and mutagenicity at lower concentrations than those used in agriculture, which emphasizes the need for more care when managing this fungicide.

Mancozeb induces cytogenotoxicity in meristematic cells of Allium cepa L

Mancozeb is a fungicide of the dithiocarbamate functional group, and it is widely used in agriculture to control various fungal diseases. Thus, studies detailing its toxicological characteristics are necessary, as the population may be exposed through the consumption of food or water contaminated with mancozeb. The aim of this study was to evaluate the cytotoxic, genotoxic, and mutagenic potentials of this dithiocarbamate using the Allium cepa L. test system as well as its cytotoxicity in erythrocytes of female rats (Rattus norvegicus). The meristematic roots of A. cepa bulbs were exposed to various concentrations of mancozeb (62.5, 125, 250, and 500 mg/L) for 24, 48, and 72 h to determine cytotoxicity by evaluating the mitotic index (MI), chromosomal aberrations (CA), and nuclear anomalies (NA) for genotoxicity analysis and micronuclei (MN) for mutagenicity analysis. Distilled water and copper sulfate (0.0006 mg/L) were used as the negative control (NC) and positive control (PC), respectively. The MI and the sum of CA and NA of all the mancozeb concentrations showed a significant difference (p ≤ 0.05) in relation to the NC, indicating possible cytotoxicity and genotoxicity induced by mancozeb. Additionally, MN significantly increased with mancozeb concentration from 250 mg/L to 500 mg/L in 24 h when compared to NC. In another study model, mancozeb showed to be cytolytic at concentrations starting from 125 mg/L. Therefore, these results indicate that mancozeb causes cytogenetic alterations and mutagenicity at lower concentrations than those used in agriculture, which emphasizes the need for more care when managing this fungicide.

Research on the bearing creep characteristics and constitutive model of gangue filling body

The creep characteristics and potential deformation patterns of gangue backfill material are crucial in backfill mining operations. This study utilizes crushed gangue from the Gangue Yard in Fuxin City as the research material. An in-house designed, large-scale, triaxial gangue compaction test system was used. Triaxial compaction creep tests were conducted on gangue materials with varying particle size distributions. Analysis was performed based on different particle sizes, stresses, and confinement pressures. The study investigates the creep characteristics of the gangue under different conditions and explores the underlying causes. It reveals the relationship between the creep deformation of gangue materials and the passage of time. Mathematical methods are applied to develop a triaxial compaction creep power law model for gangue backfill materials. Finally, the creep results are fitted using an empirical formula approach.

Optimal allocation of solar photovoltaic distributed generation for performance enhancement of electrical distribution networks considering optimal volt‐var regulation under uncertainty and high load variation

AbstractThis article proposes an optimal placement and sizing of photovoltaic (PV) power systems based distributed generation (DG) in radial electrical distribution networks considering the capability of PV inverters to regulate the voltage by optimal injecting and absorbing reactive power at the point of common coupling (PCC) using honey badger algorithm (HBA), as a recent and efficient optimization algorithm to solve the complicated optimal allocation. Several objective functions are achieved for distribution system performance enhancement: minimizing the power loss and the voltage deviation index (VDI) and maximizing the voltage stability index (VSI). Based on historical data and probabilistic models, seasonal hourly solar irradiance, ambient temperature, and load variation curves have been modeled, which simultaneously consider the light, normal, and heavy load demand. The essential components of distribution power systems have been characterized. To investigate the validity of the proposed approach, IEEE 33 and IEEE 69 BUS radial distribution test systems have been considered for power flow (PF) analyses, where Newton's Raphson method has been applied to solve the PF issue. The simulation results of different numerical scenarios have shown the effectiveness and validity of the newly proposed method to solve the optimal allocation problem considering optimal volt‐var regulation control of PV inverters compared to several valid and robust optimization algorithms.

Research on a Distributed Photovoltaic Two-Level Planning Method Based on the SCMPSO Algorithm

In response to challenges such as voltage limit violations, excessive currents, and power imbalances caused by the integration of distributed photovoltaic (distributed PV) systems into the distribution network, this study proposes at two-level optimization configuration method. This method effectively balances the grid capacity and reduces the active power losses, thereby decreasing the operating costs. The upper-level optimization enhances the distribution network’s capacity by determining the siting and sizing of distributed PV devices. The lower-level aims to reduce the active power losses, improve the voltage stability margins, and minimize the voltage deviations. The upper-level planning results, which include the siting and sizing of the distributed PV, are used as initial conditions for the lower level. Subsequently, the lower level feeds back its optimization results to further refine the configuration. The model is solved using an improved second-order oscillating chaotic map particle swarm optimization algorithm (SCMPSO) combined with a second-order relaxation method. The simulation experiments on an improved IEEE 33-bus test system show that the SCMPSO algorithm can effectively reduce the voltage deviations, decrease the voltage fluctuations, lower the active power losses in the distribution network, and significantly enhance the power quality.

A Single Microfluidic Device Approach to Direct Isolation, Purification, and Amplification of cfDNA from Undiluted Plasma

Circulating cell free DNA (cfDNA) is a valuable source of biomarkers for a range of medical applications including detection and monitoring of diseases. Currently, cfDNA sequence analysis must take place in a laboratory setting, due to the multiple steps required for processing including collection, purification, amplification, and analysis. Developing a point-of-care test system that combines these steps would simplify DNA processing thereby increasing diagnostic screening accessibility and enabling real-time monitoring for individual patients. Here, we have developed a system that combines multiple cfDNA processing steps into a single microfluidic-based device. This includes cfDNA collection directly from undiluted human plasma followed by purification and on chip amplification. A microelectrode array embedded within the microfluidic chip collected cfDNA through the creation of dielectrophoretic (DEP) forces. DEP utilizes differences in dielectric properties between cfDNA and plasma to preferentially induce a force on cfDNA. We then achieved on-chip amplification of collected DNA by designing a thermal cycling system to enable polymerase chain reaction (PCR) on the chip. This successfully consolidated the most labor-intensive steps of collection, purification, and amplification into a single device. Compared to elution of cfDNA for off-chip amplification, our on-chip PCR method improved the lower limit of detection by 3-fold and improved the total DNA yield by 5-fold. Furthermore, we demonstrate its clinical diagnostic potential by detecting KRAS mutations from a pancreatic ductal adenocarcinoma patient using only 60µL of plasma. This paves the way for future development of a fully self-contained system facilitating the rapid detection of mutations in cfDNA.

Study on the fractional creep model of roadway filling paste under the coupling effect of pore water pressure and stress

Filling paste in deep roadways is significantly affected by groundwater, especially high pore water pressure, which increases the complexity and variability of the filling paste's mechanical properties. To explore the creep characteristics and long-term stability of filling paste under varying pore water pressure, the MTS815.03 test system is used to conduct creep tests under different pore water pressures and stress. Then the creep deformation of the filling paste under the complex pressure field of static pore water pressures is analyzed. Finally, through the one-to-one correspondence between the numerical simulation of the creep model and the characteristic points of the creep curve, a method for determining the creep parameters under the complex pressure field of static pore water pressures is proposed. Results show that the creep test curves of filling paste under different pore water pressures and stress are in good agreement with the model curves. This shows that the creep constitutive model in this research can better reflect the whole process of creep deformation of filling paste. The result also verifies the rationality of the proposed method to determine creep model parameters. The newly proposed creep model can effectively compensate for the traditional Nishihara model, which is inability to reflect the acceleration of creep and can more accurately describe the creep characteristics of the primary and steady-state creep stages.

UX Analysis on SpeedID Application Using Usability Testing Method and System Usability Scale

The SpeedID application is a smart city application developed by a subsidiary of PT Bamboomedia Cipta Persada, namely PT Inovasi Solusi Nusantara since 5 years ago. The SpeedID application wants to present a solution to the city's problems to become a new digital identity for the smart city community. Because it was only developed 5 years ago, the SpeedID application is classified as a new application and has never been analyzed for usability before. Usability analysis is carried out to improve user experience, so that the SpeedID application can be accepted and used more easily by users. This research uses Usability Testing method with Performance Measurement and Retrospective Think Aloud (RTA) techniques and System Usability Scale (SUS). The results obtained are the SpeedID application has a quality that cannot be said to be effective, efficient and meet user satisfaction. In addition, the average score of the System Usability Scale (SUS) Questionnaire is 70.33. The score is rated "C" with an adjective rating of "Good" with the acceptance range included in the "Marginal" category, and finally the net promotion score (NPS) is included in the "Passive" category, which explains that the use of the SpeedID application gets an assessment that is still marginalized by its users. This shows that the SpeedID application still urgently needs correction to improve quality to its users and design improvements also need to be made so that the SpeedID application is even better at meeting user expectations in the future.

Real-time hybrid test method for floating wind turbines: Focusing on the aerodynamic load identification

Since the development of floating wind turbine (FWT) shows a rapid trend towards larger capacity and larger rotor size, the traditional full-model basin test method has encountered limits. Especially the balance between the wind generation system (WGS) size and scale ratio of the model FWT system. Under such circumstances, the hybrid model test method provides the possibility to improve the FWT model test. In the hybrid model test method, the original physical model system is divided into physical subsystem and numerical subsystem, and the data acquisition and process module between the 2 subsystems plays an important role. In this paper, a framework of real-time hybrid test (RTHT) system is setup firstly, which combines physical model wind turbine and motion platform. The damping modification to the corresponding numerical code is applied to improve the motion calculation accuracy. Delay implementation is employed to avoid motion divergence. Then a simulation loop is setup in numerical environment to study the identification of aerodynamic load. The influence of identification accuracy to the RTHT result is analyzed. Lastly, the dual-accelerometer method of aerodynamic load identification is employed in the proposed RTHT system. Decay tests and irregular wave only tests are carried out to validate the aerodynamic load identification method. The capability and potential of the RTHT method of floating wind turbine model test is preliminary proved in the work of this paper.

Flow shear force destabilizes carotid plaques by affecting CHOP and GRP78 proteins

BackgroundVarious factors, including blood, inflammatory, infectious, and immune factors, can cause ischemic stroke. However, the primary cause is often the instability of cervical arteriosclerosis plaque. It is estimated that 18-25% of ischemic strokes are caused by the rupture of carotid plaque.1 Plaque stability is crucial in determining patient prognosis. Developing a highly accurate, non-invasive, or minimally invasive technique to assess carotid plaque stability is crucial for diagnosing and treating stroke.Previous research by our group has demonstrated that the expression levels of CHOP (C/EBP homologous protein) and GRP78 (glucose-regulated protein 78) are correlated with the stability of atherosclerotic plaques.2 ObjectThis research assesses changes in GRP78 and CHOP expressions in human umbilical vein endothelial cells(HUVEC) following experiments within the hemodynamic influencing factors test system. Additionally, it includes conducting an empirical study on the impact of blood flow shear force on the stability of human carotid atherosclerotic plaques. The objective is to explore the implications of blood flow shear force on the stability of carotid atherosclerotic plaques. MethodThe hemodynamic influencing factors test bench system was configured with low (Group A, 4 dyns/cm²), medium (Group B, 8 dyns/cm²), and high shear force groups (Group C, 12 dyns/cm²). Relative expression levels of GRP78 and CHOP proteins in human umbilical vein endothelial cells were measured using Western blot analysis, and quantitative analysis of GRP78 and CHOP mRNA was conducted using RT-qPCR. Meanwhile, plaques from 60 carotid artery patients, retrieved via Carotid Endarterectomy (CEA), were classified into stable (S) and unstable (U) groups based on pathological criteria. Shear force at the carotid bifurcation was measured preoperatively using ultrasound. Western blot and RT-qPCR were used to analyze the relative expression levels of GRP78 and CHOP proteins and mRNA, respectively, in the plaque specimens from both groups. ResultExpression levels of GRP78, CHOP proteins, and their mRNAs were assessed in groups A, B, and C via Western blot and RT-qPCR. Results showed that in the low-shear group, all markers were elevated in group A compared to groups B and C. Statistical analysis revealed significantly lower shear forces at the carotid bifurcation in group U compared to group S. In group U plaques, GRP78 and CHOP expressions were significantly higher in group U than in group S. ConclusionBlood flow shear forces variably affect the expression of GRP78 and CHOP proteins, as well as their mRNA levels, in vascular endothelial cells. The lower the shear force and fluid flow rate, the higher the expression of GRP78 and CHOP, potentially leading to endoplasmic reticulum stress(ERS), which may destabilize the plaque.

The Cognitive Function and Taekwondo-Specific Kick Performance of Taekwondo Athletes at Different Hydration Statuses.

Successful participation in taekwondo (TKD) requires athletes to possess quick decision-making abilities and demonstrate technical proficiency during competition. Dehydration, occurring during both training and competition, is widely recognized to have various negative effects. This study investigated the impact of different levels of dehydration on cognitive function, as measured by the Vienna Test System, and the specific performance of kicking techniques among TKD athletes. Using a randomized crossover design, 12 participants were involved in the study. Before and after 1 hour of training at 80% of maximal heart rate, participants were weighed and provided urine samples. All participants were randomly assigned to 3 different hydration conditions: the euhydrated (EUH) group had unrestricted access to fluid consumption, while the hypohydrated (HYP) and severelyHYP (S-HYP) groups experienced reductions of 2.0% and 4.0% of their initial body weight, respectively. The EUH group exhibited better reaction speed in reaction-time test-form S1 than the HYP and S-HYP groups. Notably, the EUH group demonstrated a significantly higher success rate in the front-side kick (EUH 98%, HYP 90%, S-HYP 88%; P < .05). However, the success rates of back roundhouse kick and free head kick were similar among the 3 statuses. Furthermore, postexercise heart rates were found to be significantly higher in the HYP and S-HYP groups compared with the EUH group. This study provides insight into the negative effects of dehydration on cognitive function and TKD-specific performance. It is recommended that TKD athletes maintain optimal hydration levels during training and competition to ensure optimal performance.

A Remote Intelligent Check and Discharge Test System for Substation Battery Pack Based on Raspberry Pi

Abstract Regular check and discharge test of the battery pack is an effective means to ensure the normal state of the battery and timely detect outdated batteries. However, conventional manual check and discharge test methods are costly, cumbersome, and inefficient in maintenance. This article introduces a remote intelligent check and discharge test system for a substation battery pack based on Raspberry Pi. The system monitors the battery status in real-time, allowing operators to detect battery faults on time through the front-end management interface. At the same time, remote check and discharge tests can be carried out, greatly ensuring battery safety and solving the shortcomings of conventional manual methods.