TPACK is knowledge that needs to be mastered by teachers to be able to integrate technology in the learning process. The success of integrating ICT can be influenced by teacher gender. Differences in character between male and female teachers can influence teacher’s steps and ways to teach to integrate technology in learning. This study aimed to know TPACK mastery of biology teacher and how it differs between male and female biology teachers. This study involved 29 biology teacher respondents who were taken using the convenience sampling of the hypothetical population. Data collection is conducted using a test instrument consisting of 40 items. Instruments meet the logical validity test based on expert judgment. The empirical validity showed infit MNSQ 1.00 invitations and standard deviation of 0.16 which means all items were valid. Test instrument reliability results of 0.57 means that test was good enough to provide consistent measurement results. The data were analysed descriptively and inferentially through the independent sample t-test. The results of this study showed teacher TPACK mastery was good with a score of 59.15 but TK domain with score of 46.67 and TPACK domain with score of 45.83 were still fair. TPACK mastery of biology teacher was not differentiated by teacher gender with p>0.05.
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