Antioxidant Activity Test Research Articles

Physicochemical Characteristics, Fatty Acid Profile, and In Vitro Antioxidant Activity Evaluation of Sacha Inchi Seed Oil from Indonesia

Free radicals can cause damage to the structure of the dermis layer, which makes skin lose its elasticity and leads to the formation of wrinkles. A strategy to prevent this problem is by using antioxidants. A plant that has been reported to contain good antioxidant activity is sacha inchi seed (Plukenetia volubilis L.); apart from that, its oil has quite a high omega-3 content and potentially can act as an anti-aging agent stimulating the skin-cell-regeneration process, maintaining skin moisture and elasticity and stimulating collagen production. This research aims to analyze the physicochemical characteristics and determine the fatty acid profile, the levels of vitamins A, D, and E, and the antioxidant activity of sacha inchi seed oil. This research was conducted through eight main stages: sacha inchi seed extraction, quality parameters checking, phytochemical screening, determining fatty acid profiles, vitamin analysis, antioxidant activity tests, microbiological contamination tests, and heavy-metal contamination tests. In this study, quality inspection results were obtained: organoleptic form (liquid), color (yellow), odor (typical), relative density value (0.91 g/cm3), acid number (0.38 ± 0.02 mg/g), peroxide value (11.01 mEq/Kg), iodine value (179.32 g/100 g), and refractive index (1.479). The phytochemical screening results of sacha inchi seed oil were positive for containing flavonoids, triterpenoids, and steroids. The results of the fatty acid profile were omega-3 (48.5%), omega-6 (34.8%), and omega-9 (7.7%). The results of the vitamin contents analysis were vitamin A (123.42 mg/100 g), vitamin D (899.46 mg/100 g), and vitamin E (145.06 mg/100 g). The antioxidant activity test showed an IC50 value of 8.859 ppm (very strong), and the microbial and heavy-metal contamination tests were negative.

Pengaruh variasi konsentrasi tempe kedelai dan kulit pisang ambon (Musa paradisiaca) terhadap aktivitas antioksidan dan daya terima steak tempe

Background: Tempe contains bioactive compounds in the form of isoflavones, scientifically proven to have antioxidant abilities. In addition, banana peel antioxidants are quite high compared to the fruit flesh. Food products that are high in antioxidants have the potential to reduce various degenerative diseases. The combination of antioxidants from soybean tempeh and Ambon banana peel is expected to be one of the efforts to maintain health by minimizing the risk factors for chronic disease due to hypercholesterolemia.Objective: The study aimed to analyze the effect of varying soy tempeh and Ambon banana peel concentrations on tempeh steak's antioxidant activity and acceptability.Method: Research using design Quasi-Experiment, with one group posttest-only design. This research was conducted in January-March 2023. The research procedure began with making tempeh steak with the formulation of the comparison of soybean tempe: Ambon banana skin, namely F1 (30%: 70%), F2 (40%: 60%), F3 (50%: 50%), F4 (60%:40%). There were 4 experimental groups and 3 repetitions. Then, the antioxidant test was carried out using the DPPH method at the Jember State Polytechnic Bioscience Laboratory. The data was analyzed using this method by Kruskal Wallis. Acceptance test was carried out on 30 untrained panelists using the hedonic method at the Integrated Laboratory of FKM University of Jember and data analysis using the test Friedman, at a significance level of 95% CI.Results: The results of the antioxidant activity test showed no significant difference in the average antioxidant activity of tempeh steak (p= 0,951). The average results of antioxidant activity were F1 (70,61%), F2 (71.77%,) F3 (70,64%), F4 (71,44%). Acceptability test results showed significant differences in terms of aroma (p= 0,004), taste (p= 0,026), and overall (p= 0,004), but not significantly different in terms of color (p= 0,055) and texture (p= 0,157). The most preferred experimental group is F4[A2] (p= 0,004).Conclusion: The content of soy tempeh and Ambon banana skin contribute to each other in increasing the percentage of antioxidant inhibition so that it affects the increase in the antioxidant activity of tempeh steak. The higher the concentration of soy tempeh used, the higher the level of preference for the acceptance of tempeh steak. The product recommended in this study was the F4 sample with an average antioxidant activity yield of 71,44%, and the panelists preferred the F4 sample.

Phytochemical screening and antioxidant analysis on algae of the Phaeophyta Division (Sargassum sp. and Padina sp.)

Algae Division of Phaeophyta (brown algae) is a group of macroalgae that have important benefits for life because they contain bioactive compounds such as antioxidants. This study aims to identify bioactive compounds and analyze the antioxidant activity of algae of the Phaeophyta Division in the genus Sargassum sp. and Padina sp. The methodology in this study included extract preparation by maceration method using 70% ethanol, phytochemical tests using several reagents, phenolic total was carried out quantitatively, and antioxidant activity testing using the 1,1-Diphenyl-2-Pycrihydracyl (DPPH) method. The results showed that Sargassum sp. contains tannins, saponins, alkaloids, steroids, and triterpenoids. Meanwhile, Padina sp. contains only tannins. The yield of Sargassum sp. extract was 0.086% while that of Padina sp. extract was 0.065%. The total phenolic content of the Sargassum sp. extract was 419.133% while that of the Padina sp. extract was 248.467%. Antioxidant activity of Sargassum sp. extracts with IC50 10.93 mg/L and Padina sp. of 10 mg/L. The higher the total phenolic content, the higher the antioxidant activity, in which phenolic compounds are known to play a role in neutralizing free radicals, inhibiting oxidation in body cells, and can prevent cell damage.

Antioxidant Face Cream Formulation of Ethanol Extract from Soursop leaves (Annona muricata L)

Face cream is a treatment cream used to treat facial skin. One of the natural plant ingredients that can be used as an active substance in cream preparations is soursop leaves (Annona muricata L.) which have antioxidant activity. The purpose of this study was to formulate cream preparations from soursop leaf ethanol extract as well as the antioxidant activity of face cream preparations. Soursop leaves were extracted by maceration using a 96% ethanol solvent. Cream preparations in physical quality evaluation include organoleptic, pH, homogeneity, viscosity, spread power, sticking power, centrifugation, determination of emulsion type, and antioxidant activity test with DPPH method at 𝜆 511,5 nm using UV-Vis spectrophotometry. The results that organoleptic cream preparations had color differences. The pH of FI, FII, and FIII creams had an average yield of 6,0-6,6 and were homogeneous. All creams had a viscosity value of 49950 cPs and scatter power at 5,2-5,7 cm. FI, FII, and FIII creams fixed power test with an average value of 26,5-46,5 and physically stable at centrifugation tests with cream emulsion type of O/W. Based on the antioxidant activity, the facial creams of ethanol extract from soursop leaves of FI (3%), FII (5%), and FIII (7%) had IC50 values of 80,64, 46,06, and 10,91 ppm respectively. So this study concluded that the facial cream of ethanol extract from soursop leaves at FI, FII, and FIII meet the physical stability requirements based on organoleptic, pH, homogeneity, viscosity, scatter power, clinging power, centrifugation, and emulsion type tests. FI cream had strong antioxidant activity (IC50 value of 80,64 ppm), then FII and FIII had very strong antioxidant activity (IC50 value of 46,06 and 10,91 ppm. Keywords: Cream, Formulation, Antioxidant, Ethanol extract, Soursop leaves

SENSORY QUALITY AND ANTIOXIDANT ACTIVITY OF SHALLOTS CRISP ENRICHED RED BEETROOT PASTE (Beta vulgaris L.)

Shallots crisp is one type of traditional Indonesian food that is favoured by the wider community. Currently, public awareness of healthy food is increasing, including in choosing snacks. Red beetroot is a tuber plant that has the advantage of being a natural coloring agent and a source of antioxidants. Aims of this study were to determine the antioxidant activity and sensory test of shallots crips with the addition of beetroot paste at various concentrations. This study used a non-factorial Completely Randomized Design. The treatment used was making shallots crips using 200 g of flour added with a concentration of beetroot paste with a concentration of F1=20%, F2=30%, F3=40%, F4=50%, and F5=60%. Repetition was carried out 3 times to obtain 15 trials. The analysis carried out was the hedonic test, the description test on organoleptic including color, aroma, taste and texture as well as testing the antioxidant activity and water content of the Shallots cripss added with red beetroot paste. The results showed that the addition of red beetroot paste had a significant effect (p<0.05) on antioxidant activity, water content, description test and hedonic test for color, aroma, taste and texture. This research recommends F3 treatment as the best treatment alternative considering its antioxidant activity (58.26%); moisture content (3.40%); color description 4.44 (purplish red), onion and beetroot cake aroma description 3.52 (normal/typical), savory taste description 3.27 (normal/typical), texture description 3.29 (normal/typical) and hedonic with a color score of 3.99 (likes), aroma 3.81 (likes), taste 4.27 (likes) and texture 4.10 (likes).

Effect of Carboxyl Methyl Cellulose Addition and Heating Time on The Production of Keribang Barbant Jam (Dioscorea alata L.)

Keribang tubers are a prospective local food and can be used as food for claims and food diversification. Anthocyanins from keribang tubers can be processed into food products, one of them is made into jam. Jam is a food product with a gel consistency, therefore a thickening agent such as Carboxyl Methyl Cellulose (CMC) is needed. The purpose of this study was to determine the effect of adding CMC and heating time as well as the best combination to the physicochemical characteristics of keribang tuber jam. This research design used a Randomized Block Design (RAK) with two treatment factors, consisting of 3 treatments of CMC (0.5%; 0.75%; and 1%) and 2 treatments of heating time (15 minutes and 20 minutes), with 4 replications. Parameters observed were water content, total polyphenols, total dissolved solids, antioxidant activity, smearing power, and organoleptic tests. The best treatment was tested using the effectiveness index test. The results showed that CMC concentration treatment and heating time did not significantly affect the water content, total dissolved solids content, antioxidant activity, and spread ability, but significant on the total polyphenol content value in keribang tubers jam. The organoleptic test showed that the panelists preferred the keribang tubers jam with a CMC concentration of 0.5% and a heating time of 15 minutes in terms of color and texture. Based on the effectiveness index test, the best treatment for keribang tubers jam was 1% CMC concentration and 20 minutes of heating time

Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry

Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).

IDENTIFIKASI METABOLIT SEKUNDER DAN UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN SAMBUNG NYAWA (Gynura procumbens (Lour.) Merr).

Sambung nyawa (Gynura procumbens (Lour.) Merr) is one of the plants used in traditional medicine. This has life-saving properties such as preventing polyps, dysentery, hypertension, diabetes, and cancer. In this study, the secondary metabolite content and antioxidant activity of sambung nyawa leaf extract were tested and its antioxidant activity. The extraction process was carried out by multilevel maceration using three different solvents, starting with hexane, ethyl acetate, and methanol. Secondary metabolite content test results showed that ironwood leaves contain flavonoids, phenolics, steroids, terpenoids, and alkaloids. The result of the antioxidant activity test using the DPPH method (1,1-Difenil-2- pikrilhidrazil) showed that the methanol extract showed very strong activity with an IC50 value of 15,01 mg/L, while the ethyl acetate showed moderate antioxidant activity with an IC50 value of 147,04 mg/L, and the hexane extract showed antioxidant activity with an IC50 value of 361,66 mg/L.

Hippobroma longiflora L Leaves as a Natural Inhibitor of Cataract Progression: A Comprehensive Study Integrating Ethanol Extract, HPLC, and Molecular Docking Approaches

Cataract remains the highest preventable cause of blindness in the world. The H. longiflora plant (Hippobroma longiflora L) is a plant that has empirically been widely used to treat a variety of eye diseases including cataract. This plant has high antioxidant activity due to its high phenolic and flavonoid content. The H. longiflora plant was extracted using an ethanol solvent and its compound content was analyzed using GCMS and phytochemistry analysis. The extracts obtained were tested with TPC, TFC, antioxidants and quercetine compound with HPLC. From phytochemical and GCMS analysis, it was found that triterpenoid compounds were not found in the sample, and the compound 1-Isobutyl-2,5-dimethyl-4-phenyl-piperidin-4-ol was the most abundant (37.77%), as well as 4-Penten-1-ol, 5-phenyl-3-pperidino (1,30%) were the least abundant in the ethanol extract of the H. longiflora leaves extract. The total phenolic, flavonoid and HPLC results of quercetin were 139.21 mg GAE/g; 11.48 mg QE/G and 0.009 mg/G. Meanwhile, in an antioxidant activity test, it was found that the ethanol extract of the H. longiflora leaves has very strong antioxidants. This finding revealed the potential of ethanol extracts of H. longiflora leaves as a cataract inhibitor through its process as an antioxidant.

Profiling of Single Garlic Extract Microencapsulation: Characterization, Antioxidant Activity, and Release Kinetic

Single garlic is known to have many benefits as an alternative therapy for various types of metabolic syndrome. The bioactive compounds, allicin and alliin, in garlic are unstable and easily degraded in digestion. Chitosan-alginate microencapsulation is thought to increase stability and protect active compound so its therapeutic effect is more optimal. This study aimed to characterize the microencapsulation chitosan-alginate of single garlic extract (MCA- SGE), as well as to examine the antioxidant activity and kinetic release of MCA-SGE in vitro. The research procedure includes the steps of single garlic extraction, preparation of MCA-SGE, characterization of MCA-SGE (PSA, SEM, and FTIR) as well as biological testing of MCA-SGE through antioxidant activity and kinetic release tests. PSA results showed the mean particle size of MCA-SGE was 439.0 ± 1.9 nm or 0.4 m with a polydispersity index (PDI) value of 0.579 ± 0.046 and a zeta potential value of 15.4 ± 0.3 mV. The SEM results showed that the morphology of MCA-SGE was spherical with a smooth surface and a micrometre size of 0.4 - 0.7 µm. The FTIR results describe a shift in absorption and addition of SGE functional groups after encapsulation. The results of the antioxidant activity test showed the antioxidant activity of MCA-SGE was 65%, while SGE was 55%. The results of the kinetic release showed that more allicin and alliin were released by SGE than MCA-SGE during the 4-hour kinetic release simulation. MCA-SGE has the potential to be used as a drug delivery system with controlled release.

Effect of Low Concentration of Nitroxides on SH-SY5Y Cells Transfected with the Tau Protein

Nitroxides, stable synthetic free radicals, are promising antioxidants, showing many beneficial effects both at the cellular level and in animal studies. However, the cells are usually treated with high millimolar concentrations of nitroxides which are not relevant to the concentrations that could be attained in vivo. This paper aimed to examine the effects of low (≤10 μM) concentrations of three nitroxides, 2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO), 4-hydroxy-TEMPO (TEMPOL) and 4-amino-TEMPO (TEMPAMINE), in pure chemical systems and on SH-SY5Y cells transfected with the human tau protein (TAU cells), a model of chronic cellular oxidative stress, and transfected with the empty plasmid (EP cells). All nitroxides were active in antioxidant-activity tests except for the 2,2′-azinobis-(3-ethylbenzthiazolin-6-sulfonate) radical (ABTS•) decolorization assay and reduced Fe3+, inhibited autoxidation of adrenalin and pyrogallol and oxidation of dihydrorhodamine123 by 3-morpholino-sydnonimine SIN-1. TEMPO protected against fluorescein bleaching from hypochlorite, but TEMPAMINE enhanced the bleaching. Nitroxides showed no cytotoxicity and were reduced by the cells to non-paramagnetic derivatives. They decreased the level of reactive oxygen species, depleted glutathione, and increased mitochondrial-membrane potential in both types of cells, and increased lipid peroxidation in TAU cells. These results demonstrate that even at low micromolar concentrations nitroxides can affect the cellular redox equilibrium and other biochemical parameters.

Pinobanksin from peony seed husk: A flavonoid with the potential to inhibit the proliferation of SH-SY5Y.

Pinobanksin, as one of the flavonoids, has powerful biological activities but has been under-recognized. In this study, we optimized the extraction method of phragmites from peony seed shells by using organic solvent extraction. The yield of PSMS was 10.54 ± 0.13% under the conditions of ethanol volume fraction 70%, extraction temperature 70°C, material-liquid ratio 1:25 g/mL, and extraction time 60 min; the optimized PSMS could be effectively separated in S-8 macroporous resin coupled with C18. The relative content of PSMS was increased from 0.42% in PSMS to 92.53% after C18 purification; the antioxidant activity test revealed that pinobanksin could exert antioxidant ability by binding catalase (CAT) enzyme. Second, it was found that pinobanksin could effectively inhibit the proliferation of SH-SY5Y cells, mainly by binding to BCL2-associated X (BAX), B-cell lymphoma-2 (BCL-2), and cyclin-dependent Kinase 4/6 (CDK4/6) to produce more hydrogen bonds to inhibit their activities. This study confirms the medicinal potential of pinobanksin and provides the basis for the proper understanding of pinobanksin and the development of related products.

Antioxidant Activity of Mahogany Parasite Leaves (Dendrophthu pentandra (L.) Miq.) Assessed Using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) Method

Synthetic antioxidants, such as butyl hydroxyanisole (BHA), have carcinogenic (cancer-causing) adverse effects because they can cause type I alveolar damage and damage to lung endothelial genes. The objective of this study was to use the DPPH (2,2 diphenyl-1-picrylhydrazyl) method to investigate the antioxidant activity of natural substances found in the leaves of the mahogany parasite (Dendrophthu pentandra (L.) Miq.). Extraction, chromatography, and spectrophotometry are the techniques used. Maceration in stages utilizing n-hexane, ethyl acetate, and ethanol as solvents yielded mahogany parasite leaf extract. Phytochemical screening, thin layer chromatography testing, antioxidant activity testing utilizing a Visible Spectrophotometer, and estimating the IC50 were all performed on mahogany parasite leaf extract. The phytochemical screening results revealed that the samples included four types of secondary metabolites: flavonoids, tannins, saponins, and steroids. A thin layer chromatographic test revealed four well-separated stains using n-hexane and ethyl acetate eluent in a volume ratio of 5:5, showing the Rf as the value of the four secondary metabolites mentioned above. Based on the results of the experiments, it is possible to conclude that mahogany parasite leaves have strong antioxidant activity, with an IC50 of 8.47 ppm.

AKTIVITAS ANTIOKSIDAN DAN KEMAMPUAN ANTIBAKTERI EKSTRAK KULIT BUAH KENITU (Chrysophyllum cainito L) TERHADAP Staphylococcus aureus

Kenitu (Chrysophyllum cainito L) has strong antioxidant properties both in vitro and in vivo. The presence of this high antioxidant can be used as a natural agent to prevent and treat diseases associated with an imbalance of antioxidant levels in the body, such as diabetes. Some research results state that kenitu can be used as an antidiabetic. One type of complication in people with diabetes mellitus is the occurrence of bacterial infections of the skin. One type of bacteria that infects the most is Staphylococcus aureus. So far, the skin of the kenitu fruit has only been thrown away and has not been used. The purpose of this study was to determine the antioxidant activity of kenitu peel extract and to determine the ability of kenitu peel extract as an antibacterial against Staphylococcus aureus. Research methods included extracting kenitu peel by maceration using 70% ethanol, testing antioxidant activity using the DPPH method, and testing the antibacterial activity of kenitu peel extract against Staphylococcus aureus. The results of the antioxidant activity test showed an IC50 value of 126.96 ppm. Antibacterial activity testing showed a MIC value of 250 ppm and a MBC value of 500 ppm. The conclusion of this study is that kenitu peel extract has moderate antioxidant activity and kenitu peel extract is able to inhibit the growth and kill Staphylococcus aureus bacteria.

Identification of antioxidant compounds in fig leaves (Ficus carica L) fractions using LCMS-MS

The fig plant (Ficus carica L.) is one of the most popular ficus genera and is spread in tropical and sub-tropical regions throughout the world. Fig leaves have potential as antioxidants. The purpose of this study was to identify antioxidant compounds in the ethanol extract of fig leaves of the Iraqi variety. The research involved preparing extracts by maceration using 96% ethanol, 70% ethanol, and 50% ethanol, testing antioxidant activity and identifying their chemical structures using LC-MS/MS. Based on the results of the antioxidant activity test using the DPPH free radical scavenging method, it was shown that the 50% ethanol extract of fig leaves had a very strong antioxidant activity with an IC50 value of 23.36 µg/mL. The results of identification with LC-MS/MS, it is estimated that the compound that has the potential as an antioxidant in the fig leaves of the Iraqi variety is the kaempferol compound.

Compound Characterization and Evaluation of Antioxidant Potential of Ethanol Extract Spatholobus littoralis Hassk in Kolaka, Southeast Sulawesi, Indonesia

The bajakah tampala plant (Spahtolobus littoralis Hassk) has been traditionally believed by the public to cure cancer, tumors, heart disease, stroke, and diabetes. However, empirically the public does not yet know the chemical compound content and there have been no studies on biological activity tests, especially the antioxidants of bajakah tampala from Kolaka, Southeast Sulawesi. The aim of this research is a qualitative analysis of the chemical compounds of the ethanol extract of bajakah tampala roots and testing of antioxidant activity. Analysis of the chemical compounds of the ethanol extract of bajakah tampala roots using an FT-IR spectrophotometer and LC-MS/MS. The antioxidant activity of the bajakah tampala root ethanol extract was tested using the DPPH immersion method and the absorption was measured using a UV-Viss spectrophotometer and using the positive control quercetin. The results of the research showed that the ethanol extract of bajakah tampala roots had 8 chemical components that were detected, namely compound 1 apocynoside I, compound 2 quercetin, compound 3 hexosylphingosine, compound 4 3-hydroxy-7-methoxybaicalein, compound 5 momor-cerebroside I, compound 6 ambronal, compound 7 stigmastan-3,6-dione, and compound 8 trilaurin. The ethanol extract of bajakah tampala roots has an antioxidant activity of 21.75 µg/mL which is lower, compared to the antioxidant activity of quercetin of 2.91 µg/mL as a positive control, however the antioxidant activity of the ethanol extract of bajakah tampala roots is still included in the very strong category.

Technological parameters of cassava starch/carboxymethyl cellulose blend-based films added of soy lecithin and tocopherol mix

The aim was to evaluate the technological properties of films based on cassava starch/carboxymethyl cellulose added to soy lecithin and tocopherol mix. The films were prepared by casting from a cassava starch/carboxymethyl cellulose mixture and identified as control B (no soy lecithin and no tocopherol mix), L (with soy lecithin and no tocopherol mix) and LT and LT2 (with soy lecithin and tocopherol mix). Thickness, water solubility, water content, film weight, contact angle, permeability test, mechanical tests, antioxidant activity, biodegradability, color, thermogravimetry, and FTIR tests were performed. The results showed that the films containing soy lecithin (L, LT, and LT2) were more hydrophilic and had a denser structure than film B, but the LT and LT2 films were slightly yellow-green, malleable and showed few bubbles or cracks. In addition, all the films developed are biodegradable. It was also found that the antioxidant remained in the films to which it was added (LT and LT2) and that the LT2 film showed the highest antioxidant activity. It is strongly recommended for application in active food packaging.

ORGANOLEPTIC TESTS AND ANTIOXIDANT ACTIVITY OF BLACK SOYBEAN (GLYCINE SOJA L) SUPPLEMENTED TAMARILLO(SOLANUM BETACEUM CAV)

Degenerative disease refers to metabolic syndrome which occurs as a result of an unhealthy lifestyle. An unhealthy diet results in the emergence of large amounts of free radicals. High free radical content can disrupt cells and their genetic material which can result in degenerative diseases. Antioxidants are compounds that function to protect body cells from damage caused by free radicals that cause degenerative diseases. Soybean juice added with tamarillo is a food ingredient that is a source of antioxidants. The aim of the research was to obtain the best Dutch eggplant formula added to soy milk. The research method used was a Completely Randomized Design (CRD) which involved four additional treatments of Dutch eggplant, namely P1 (50 mL), P2 (55 mL), P3 (60 mL) and P4 (65 mL). Research activities were initially carried out by testing the organoleptic products using the hedonic method by 70 untrained panelists. Product testing is carried out using Then the best product testing results are continued to be tested for antioxidant content. Antioxidant testing The results of the organoleptic test research obtained the highest scores for the color attribute 5.69 (P3), aroma 5.23 (P2), taste 6.09 (P1), texture 4.76 (P1), and overall 5.81 (P1). Based on organoleptic tests, the best formula is 60 mL (P3) then the antioxidant activity test is 0.3374 ppm. The conclusion in this study shows that the best formula in terms of color, taste and overall black soybean juice added with Dutch eggplant has significant antioxidants.

Total Lactic Acid Bacteria (LAB) and Antioxidant Activity of Goat's Milk Yoghurt with The Addition Of Red Bran During Refrigeration Storage

Background: Goat milk yogurt is a functional food made from processed food of animal origin with the help of LAB fermentation, which is healthy for the body. The utilization of red rice bran flour as an additional ingredient in making yogurt can increase the level of antioxidant activity and become a food fiber content in yogurt. Purpose: This study aims to determine the effect of refrigerated storage temperatures (4℃) of goat milk yogurt with the addition of red rice bran flour on total LAB and antioxidant activity. Methods: Using a CRD with five treatments and four replications. The treatment included 0, 1, 2, 3, and 4 weeks of storage. Each treatment group will be calculated for total LAB using the TPC method and antioxidant activity testing using the DPPH method. The data obtained were analyzed using the One Way ANOVA Test followed by the Duncan Test to determine the effect between treatment groups. Results: The results showed that storage time significantly affected (p<0.01) total LAB and antioxidant activity. The results of total LAB successively from 0 to 4 weeks of storage were 8,0 x 108 CFU/ml, 7,8 x 107 CFU/ml, 7,6 x 107 CFU/ml, 6,4 x 106 CFU/ml, and 6,3 x 106 CFU/ml. The results of antioxidant activity successively from 0 to 4 weeks of storage were 534,29 mg/ml, 443,96 mg/ml, 358,61 mg/ml, 282,32 mg/ml, and 230,88 mg/ml. Conclusion: Goat’s milk yogurt with the addition of brown rice bran flour is recommended to be consumed for up to 2 weeks of storage.

The Bioactivity Potential of Acmella Paniculata Plant Extract in Antioxidant Activity by Two Different Extraction Methods

Widely used synthetic chemicals like heavy metals act as active compounds in personal care products such as shampoo, soap and cosmetics. These hazardous chemicals cause harmful threats to the environment and unintentionally lead to dangerous exposure towards human health. Thus, demands on natural organic compounds have increased nowadays. Bioactive compounds from plant extracts are well known to have intrinsic biological values that are relevant in promoting human health. There are numerous ways to extract these unique and important compounds involving techniques that are simple, environmentally friendly and efficient. The aim of this study is to highlight the biological potential of compounds from the local plant, Acmella paniculata in antioxidant activity. This small flowering shrub species is also known locally as Subang nenek. The first phase of this study involved the leaf extraction of A. paniculata performed by a common method known as Soxhlet extraction using methanol solvent and a simple centrifuged method using deep eutectic solvent (DES) which is a green solvent. Soxhlet method is a conventional procedure that takes longer time and involves specific equipments. However, this method has been used for many years as standard method due to its consistent results. Meanwhile, DES based centrifugation method is a new, innovative method for plant extraction with a fast procedure and minimal used of equipment. The second phase involved phytochemical screening and antioxidant activity testing. The phytochemical screening for A. paniculata leaf extract showed noticeable secondary metabolites such as alkaloids, tannins, terpenoids and steroids. This plant showed good pharmacological values in antioxidant activity using DPPH free radical scavenging assay. The antioxidant percentage of DES extract showed higher potential results (56.11±3.54) compared to the Soxhlet extract (39.15±23.99) in leaf. Overall, the DES extraction is promising method in increasing the yield of antioxidant compounds as well as provided a simple and rapid technique in plant extraction.