Exposure to the herbicide atrazine has been shown to have deleterious effects on human and animal reproduction. To determine whether atrazine influences the brain-pituitary-testicular axis directly or indirectly, the present study examined the toxic effects of atrazine on fertility potential by assessing gonadal hormones, testicular function indices, sperm quality, and oxido-inflammatory markers in rats. Twelve animals were grouped into two groups; control and atrazine. The control group received oral administration of olive oil (2 mL/kg), while the atrazine group received 120 mg/kg of atrazine. Treatments were daily and lasted for 7 days. Upon treatment cessation, rats were necropsied for biochemical and histopathological analyses. The biochemical function indices in the rat brain, testis, and epididymis decreased significantly in the atrazine group. Atrazine exposure led to decreases in gonadal hormonal concentrations, semen quality parameters, and testicular function indices compared with the control. Furthermore, there was a marked increase in oxidative stress and inflammatory markers as well as degeneration of the histo-architecture in atrazine-treated rats. Overall, atrazine exposure impaired sperm quality, led to increased inflammation and oxidative stress, and decreased the activity of the brain-pituitary-testicular axis via endocrine disruption.