With the advancement of technology, dry vacuum pumps are widely used in aerospace, semiconductor, chemical and pharmaceutical and scientific equipment due to their clean and oil-free advantages. Usually the pumped medium contains different types of gases, dry Roots vacuum pumps are prone to unstable performance, which can adversely affect process efficiency. To investigate the influence of gas types on the pumping performance of Roots vacuum pump, experimental research was conducted on self-designed dry vacuum pump theoretical verification platform and test pump. The gas mixture of 70 % nitrogen and 30 % helium was tested using 2-lobe, 3-lobe and 5-lobe Roots while numerical simulations were conducted on air, nitrogen, helium and hydrogen. The experimental results indicate that the gas type affects the pumping speed of the Roots pump. In addition, the numerical simulation results show that hydrogen has the greatest influence on the pumping performance of the Roots; the pumping speed shows ‘W’ type fluctuation in one cycle when the 3-lobe Roots pumps hydrogen. The pumping speed of the 5-lobe Roots is most affected by changes in gas types. This study is of guiding significance for the application and design of dry Roots vacuum pumps in different process environments.