Test Reagents Research Articles

Evaluation of efficiency and sensitivity of 1D and 2D sample pooling strategies for SARS-CoV-2 RT-qPCR screening purposes

To increase the throughput, lower the cost, and save scarce test reagents, laboratories can pool patient samples before SARS-CoV-2 RT-qPCR testing. While different sample pooling methods have been proposed and effectively implemented in some laboratories, no systematic and large-scale evaluations exist using real-life quantitative data gathered throughout the different epidemiological stages. Here, we use anonymous data from 9673 positive cases to model, simulate and compare 1D and 2D pooling strategies. We show that the optimal choice of pooling method and pool size is an intricate decision with a testing population-dependent efficiency-sensitivity trade-off and present an online tool to provide the reader with custom real-time 1D pooling strategy recommendations.

Meta-analysis of the robustness of COVID-19 diagnostic kit performance during the early pandemic

BackgroundAccurate detection of SARS-CoV-2 is necessary to mitigate the COVID-19 pandemic. However, the test reagents and assay platforms are varied and may not be sufficiently robust to diagnose COVID-19.MethodsWe reviewed...

Brucellosis testing patterns at health facilities in Arusha region, northern Tanzania.

Brucellosis is listed as one of six priority zoonoses in Tanzania's One Health strategic plan which highlights gaps in data needed for the surveillance and estimation of human brucellosis burdens. This study collected data on current testing practices and test results for human brucellosis in Arusha region, northern Tanzania. Retrospective data were extracted from records at 24 health facilities in Arusha region for the period January 2012 to May 2018. Data were captured on: the test reagents used for brucellosis, procurement and testing protocols, the monthly number of patients tested for brucellosis and the monthly number testing positive. Generalised linear mixed models were used to evaluate relationships between health facility characteristics and the probability that brucellosis testing was conducted in a given month, and the proportion of individuals testing positive. Four febrile Brucella agglutination tests were used widely. The probability of testing for brucellosis in a given month was significantly associated with an interaction between year of testing and facility ownership. Test probability increased over time with more pronounced increases in privately owned as compared to government facilities. The proportion of individuals testing positive for brucellosis was significantly associated with facility type and district, with individuals tested in hospitals in Meru, Monduli and Ngorongoro districts more likely to test positive. Febrile Brucella agglutination tests, known for their poor performance, were the mainstay of brucellosis testing at health facilities in northern Tanzania. The study indicates that historical data on human brucellosis in Arusha and other regions are likely to provide an inaccurate measure of true disease burden due to poor performance of the tests used and variation in testing practices. Measures to address these identified shortcomings could greatly improve quality of testing and surveillance data on brucellosis and ultimately inform prevention and control of this priority disease.

IDENTIFICATION OF ORGANIC COMPOUNDS AS CARBOHYDRATES AND CHEMICALS CONTAINED IN THEM

This study aims to observe the structure of some carbohydrates through their reaction properties with multiple test reagents and know and understand various carbohydrate tests. Research methods used Research methods used are analytics with experimental design (pre experimental), Object of research is milk, honey, sago, flour and amylum. Then to five samples each added with NaOH, HCL, Aquades to find out if the five samples contain carbohirat or not.
 The results showed that Carbohydrates are nutrients in the form of organic compounds consisting of carbon atoms, hydrogen, and oxygen used as energy-forming materials. In this carbohydrate study, an iodine test was conducted to prove the presence of carbohydrate content in 5 samples of foodstuffs, namely iodine test on sago, honey, amylum, flour and milk. In the first experiment milk added NaOH, HCL, aquades the result did not change the layer and color, then the second experiment on honey in the pond NaOH, HCL, Aquades the result is two layers and the change of color to blackish yellow, then the third experiment on flour added NaOH, HCL, Aquades the result there are two layers and discoloration in each organic compound mixed in the sample, next experiment to the fourth on sago added NaOH, HCL,

Assessment of Potential Benefits of Functional Food Characteristics of Beetroot Energy Drink and Flavored Milk.

Objective This study was designed to determine the antioxidant activity of the extracts of beetroot by using beetroot energy drink and flavored milk (products). Material & Methods. This experimental trial was conducted at Jinnah University for Women, Pakistan, under the approval of local institutional review board number JUW/DFST/RCB010/2020. All the materials such as beetroot, carrot, cucumber, and lemon were obtained commercially from which two products were formulated: beetroot energy drink (sample1) and flavored milk (sample 2). These formulated products were evaluated for quality analysis (pH and brix), phytochemical screening using the Keller-Kiliani test, Salkowski's test, Alkaline reagent test, lead acetate test, ferric chloride test, protein test, quantitative test of phenol, antioxidant activity, sensory analysis, and shelf life study. The paired t-test was applied to detect significant differences between two samples. Results The phytochemical analysis revealed that cardiac glycosides, phytosterol, flavonoids, and terpenoids were found in both energy booster drink (EBD) and flavored milk (FM) except phenolic compounds that were found only in EBD. The antioxidant capacity of beetroot juice was far greater than FM. The statistical sensorial analysis of FM and EBD reported a significant mean difference between most of the groups with p < 0.0001. Conclusion This study concludes that energy drinks having beetroot indicated higher antioxidant capacity than flavored milk. The nutraceutical products (energy booster drink and flavored milk) containing beetroot are enriched with optimum quantities of proteins and fats and low carbohydrates at a stable pH with an adequate total energy content.

Mechanism of interaction between mixed collectors and low-rank coal: An experimental and simulation study

ABSTRACT In this paper, Ordos low-rank coal was taken as the research object, methyl oleate and diethyl phthalate were used as test reagents, and the differences of coal slime flotation indexes under single collector and mixed collector conditions were compared and analyzed by the batch flotation testing of fine coal. Through DFT calculation, molecular dynamics simulation and Fourier transform infrared spectroscopy (FTIR) test, the mechanism of mixed collector improving the flotation index of low-rank coal was investigated. The results show that the flotation ability of single ester collector for low-rank coal is better than that of traditional oil collector, and the collector can further improve the flotation index of coal slime after mixing. There is a certain internal relationship between the frontier orbital energy and adsorption energy of collector molecules and their harvesting performance. When the single collector reaches adsorption equilibrium on the coal surface, the spatial orientation of the collector molecule is ‘horizontal’ on the coal surface, and the mixed collector is ‘vertical’ on the coal surface.

China's NMPAperspective on the clinical performance of SARS-CoV-2 antigen test reagents.

The COVID-19 pandemic continues to spread all over the world. In the process of emergency use authorization, the Center for Medical Device Evaluation of the China National Medical Products Administration issued ‘Key Points of Technical Review for the Registration of SARS-CoV-2 Antigen/Antibody Detection Reagents’ as the guidance of registration of antigen and antibody test reagents for the industry. In this document, clinical evaluation requirements of antigen detection reagents are elaborated. Based on the Key Points document and the authors’ review practice, this article explains the evaluation methods and requirements of clinical performance of SARS-CoV-2 antigen-detecting rapid diagnostic tests, then analyzes the application scenarios and intended use of antigen detection reagents.

Validation of rapid antibody (IgG-IgM) test kit for SARS-CoV-2 infection in Qatar.

Background: In response to the growing coronavirus disease 2019 (COVID-19) pandemic and the shortage of laboratory based molecular testing capacity and reagents, multiple diagnostic test manufacturers have developed rapid and easy to use devices to facilitate testing outside laboratory settings. These kits are either based on detection of proteins from SARS-CoV-2 virus or detection of antigen or human antibodies generated in response to the infection. However, it is important to understand their performance characteristics and they must be validated in the local population setting.Design and methods: The objective is to assess the validity of the rapid test for IgG and IgM immunoglobulins compared to the current gold standard reverse transcription polymerase chain reaction (RT-PCR) test. A total of 16951 asymptomatic individuals were tested by the Ministry of Public Health track-and-trace team using both rapid immunodiagnostic test and RT-PCR as part of screening across various random settings with potential risk of community interaction prior to gradual lifting of restrictions in Qatar. Rapid test was considered to be posiive if both IgG and IgM are positive, while only IgG/IgM positive was considered as rapid test negative. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated.Results: The sensitivity of rapid test kit was found to be 0.9%, whereas the specificity was found to be 97.8%. the PPV was found to be 0.3% whereas the NPV was found to be 99.4%.Conclusions: Based on the outcome and results of the study, it appears that the sensitivity and PPV of the rapid antibody test are low. As such, this test is not recommended for use to assist in taking clinic-based decisions or decisions related to quarantine/isolation. Significance for public health It is important to understand the performance characteristics of the Rapid Antibody (IgG-IgM) kits and they must be validated in the local population setting before they can be recommended for use.

Could pooled samples method affect SARS-CoV-2 diagnosis accuracy using BGI and Sansure-Biotech RT-PCR kits used in Gabon, Central Africa?

This study aims at establishing specimens pooling approach for the detection of SARS-CoV-2 using the RT-PCR BGI and Sansure-Biotech kits used in Gabon. To validate this approach, 14 positive samples, stored at -20°C for three to five weeks were analyzed individually (as gold standard) and in pools of five, eight and ten in the same plate. We created 14 pools of 5, 8 and 10 samples using 40 μL from each of the selected positive samples mixed with 4, 7 and 9 confirmed negative counterparts in a total volume of 200 μL, 320 μL and 400 μL for the pools of 5, 8 and 10 respectively. Both individual and pooled samples testing was conducted according to the BGI and Sansure-Biotech RT-PCR protocols used at the Professor Daniel Gahouma Laboratory (PDGL). Furthermore, the pooling method was also tested by comparing results of 470 unselected samples tested in 94 pools and individually. Results of our experiment showed that using a BGI single positive sample with cycle threshold (Ct) value of 28.42, confirmed by individual testing, detection occurred in all the pools. On the contrary samples with Ct >31 were not detected in pools of 10 and for these samples (Ct value as high as 37.17) their detection was possible in pool of 8. Regarding the Sansure-Biotech kit, positive samples were detected in all the pool sizes tested, irrespective of their Ct values. The specificity of the pooling method was 100% for the BGI and Sansure-Biotech RT-PCR assays. The present study found an increase in the Ct values with pool size for the BGI and Sansure-Biotech assays. This trend was statistically significant (Pearson’s r = 0.978; p = 0,022) using the BGI method where the mean Ct values were 24.04±1.1, 26.74±1.3, 27.91±1.1 and 28.32±1.1 for the individual, pool of 5, 8 and 10 respectively. The testing of the 470 samples showed that one of the 94 pools had a positive test similar to the individual test using the BGI and Sansure-Biotech kits. The saving of time and economizing test reagents by using the pooling method were demonstrated in this study. Ultimately, the pooling method could be used for the diagnosis of SARS-CoV-2 without modifying the accuracy of results in Gabon. We recommend a maximum pool size of 8 for the BGI kit. For the Sansure-Biotech kit, a maximum pool size of 10 can be used without affecting its accuracy compared to the individual testing.

A method validation for the hemoglobin chemical reagent test strip with the addition of ethylenediaminetetraacetic acid to increase the selectivity of this presumptive test for residual blood

Tetramethylbenzidine based chemical reagent test strips are often used in forensic science as a presumptive test for blood. These tests are designed as urinalysis test strips and include brands such as Combur®, HENSOTest®, Hemastix®, MultiStix® and Chemstrip®. They are used because they are simple to apply, stable, temperature tolerant and cost effective. The addition of a chelating agent, ethylenediaminetetraacetic acid increases the selectivity of this presumptive test for blood. This is a method validation for the hemoglobin chemical reagent test strip with EDTA. A range of substances, metal compounds, chemical solutions, blood and mixtures were tested in this method validation. The chelation with EDTA successfully prevented non-blood (false) positive results from all the substances tested and consistently produced a positive result for blood on a variety of surfaces. This study has shown that this method is capable of discriminating a blood stain on copper metal surfaces and eliminate the positive results generated by clean-up solutions such as hydrogen peroxide, which usually produce a positive result for most other presumptive tests for blood. This modified method is a simple, effective and reliable test for blood stains. A variety of variations were evaluated in this study. The simplest method of application was spraying the surface of the stain with a 0.5 M EDTA solution and testing the surface of the stain, and only requires a spray bottle of 0.5 M EDTA and the chemical reagent test strip. This spray approach is rugged and can be applied to horizontal, vertical and underside surfaces and requires little additional training. Overall, this study provides the forensic science community with an improved method more easily used, stored, transported and selective for blood, than luminol and safer than TMB.

Ascorbic acid specifically reduces the misclassification of nonirritating reactive chemicals in the OptiSafe™ macromolecular eye irritation test

Recently, we showed that the addition of physiological concentrations of ascorbic acid, a tear antioxidant, to the OptiSafe™ macromolecular eye irritation test reduced the false-positive (FP) rate for chemicals that had reactive chemistries, leading to the formation of reactive oxygen species (ROS) and molecular crosslinking. The purpose of the current study was to 1) increase the number of chemicals tested to comprehensibly determine whether the antioxidant-associated reduction in OD is specific to FP chemicals associated with ROS chemistries and 2) determine whether the addition of antioxidants interferes with the detection of true positive (TP) and true negative (TN) ocular irritants. We report that when ascorbic acid is added to the test reagents, retesting of FP chemicals with reactive chemistries show significantly reduced OD values (P < 0.05). Importantly, ascorbic acid had no significant effect on the OD values of TP or TN chemicals regardless of chemical reactivity. These findings suggest that supplementation of ascorbic acid in alternative ocular irritation tests may help improve the detection of TN for those commonly misclassified reactive chemicals.

Evaluation of Peronema canescens Leaves Extract: Fourier Transform Infrared Analysis, Total Phenolic and Flavonoid Content, Antioxidant Capacity, and Radical Scavenger Activity

BACKGROUND: Peronema canescens (Sungkai) leaves have been popular in Indonesia which contain various bioactive compounds with empirical therapeutic efficacy in dealing with COVID-19 and various other diseases. Total phenolic and flavonoid content and antioxidant activity using the DPPH method from P. canescens leaf extract have not been studied much. AIM: This research has several objectives. The first is to compare the results of qualitative phytochemical analysis of the ethanol extract of the leaves of P. canescens (EEPL). The second is to measure the total phenol and flavonoid content. The third is to test the FTIR and antioxidant activity of the ethanol extract of P. canescens leaves in vitro using the DPPH method. METHODS: Fresh plant material and simplicia, ethanol extract extracted by maceration method using 96% ethanol as solvent from P. canescens. The Dragendorff’s and Mayer test carried out the qualitative phytochemical analysis, FeCl3 test, Salkowski method, Liebermann–Burchard method, foam test, and NaOH reagent. The total phenolic and flavonoid levels were tested using the Folin–Ciocalteu method. In vitro antioxidant activity was carried out using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. RESULTS: The results of qualitative phytochemical screening showed that alkaloids, flavonoids, saponins, tannins, and steroids were detected in the extract of P. canescens. The spectra from the FTIR test results showed various absorbance peak values indicating the bonding of specific functional groups, namely: 418.12, 599.94, 666.67, 1036.39, 1159.52, 1224.16, 1348.95, 1454.19, 1600.87, 1732.00, 2923.13, and 3353.01 cm-1. In the test results, total phenolic content was as much as 5.64% (mgEAG/g) and total flavonoid content of 142,247 mgEQ/g in a sample of 1 mg extract, which was equivalent to 1 mg quercetin. EEPL has antioxidant activity with the DPPH IC50 method of 116.7865 ppm. CONCLUSION: The data obtained at this time can contribute to the exploitation of P. canescens leaves in the future as one of the nutraceutical products, supplements, and herbal medicines by specific industries related to improving the health status of the world community. The higher the bioactive substance in preparation, the more significant the effect of the pharmacological efficacy response. P. canescens ethanol extract has good total phenolic content, total flavonoid content, and antioxidant action.

Ratiometric fluorescence and colorimetric dual-mode sensing platform based on carbon dots for detecting copper(II) ions and D-penicillamine.

A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions (Cu2+) andD-penicillamine (D-pen) was constructed based on carbon dots (CDs). o-Phenylenediamine (OPD) was employed as a chromogenic development reagent for reaction with Cu2+to generate the oxidation product 2,3-diaminophenazine (oxOPD), which not only emits green fluorescence at 555nm, but also quenches the blue fluorescence of CDs at 443nm via the inner filter effect (IFE) and Förster resonance energy transfer (FRET). Additionally, oxOPD exhibits obvious absorption at 420nm. Since the intense chelation affinity of D-pen to Cu2+ greatly inhibits the oxidation of OPD, the intensity ratio of fluorescence at 443nm to that at 555nm (F443/F555) and the absorbance at 420nm (A420) were conveniently employed as spectral response signals to represent the amount of D-pen introduced into the testing system. This dual-signal sensing platform exhibits excellent selectivity and sensitivity towards both Cu2+ and D-pen, with low detection limits of 0.019μM and 0.092μM, respectively. In addition, the low cytotoxicity of the testing reagents involved in the proposed sensing platform facilitates its application for live cell imaging.

Leave No Preschool-Aged Children Behind: Urogenital Schistosomiasis in Four Communities of a Metropolitan City in Central Nigeria

There have been several demands for preschool-aged children to get prophylactic chemotherapy, such as praziquantel, first due to a presumed schistosomiasis among this age group. In order to determine the prevalence and risk variables related to urogenital schistosomiasis infection among preschool-aged children, this study conducted surveys in four areas inside a major city in Central Nigeria. A total of 240 preschool-aged children were randomly selected from the four areas surveyed. Demographic data and their knowledge on the transmission of urogenital schistosomiasis were obtained by administering questionnaire to mothers and caregivers, urine samples of the recruited subjects were collected using a sterile sampling bottles and were tested for hematuria using Urino-Combi-11 reagent test strip (ACON laboratories). Subsequently, samples were screened in the laboratory for Schistosoma haematobium infection. Of the 235 preschool-aged children that returned their sample bottles, only 8.51% (20/235) were infected with urogenital schistosomiasis. Four years old subjects were the most infected 13.95% (6/43) followed by age two 10.56% (2/19) then age five with 8.93% (10/112), age three 3.70% (2/54) while no age one child was infected 0% (0/7), although variations in prevalence across the ages showed no significant difference (P˃0.05). The overall geometric mean intensity (GMI) was 1.03526 eggs/10ml of urine. The prevalence of the infection among preschool-aged children in relation to locations as well as sex showed no significant difference (P˃0.05). About 52% (122) of the preschool-aged children bath in water bodies, but only 51.1% (120) are aware that some freshwater snails are harmful. In conclusion, preschool-aged children in the metropolitan city of Lafia should not bath, wash or swim in water bodies around. More awareness should be created on the risk factors associated with the disease. Also, the government should provide sufficient potable water in the city, and prioritization of treatment of preschool-aged children with preventive chemotherapy to curb urogenital schistosomiasis prevalence among the age group is very necessary and cannot be overemphasized.

Pharmacognostical and phytochemical standardization on stem bark of Mahonia nepalensis DC.: An extrapharmacopoeial plant

Introduction: The tribal people cure hepatic-related disorders with the stem bark of Mahonia nepalensis DC. Aim: The aim of the study was to do Investigation of the Microscopic and Macroscopic Characteristics, Pharmacognostical, Physiochemical Parameters, and Thin Layer Chromatography of Stem Bark of Mahonia nepalensis DC. Materials and Methods: Mahonia nepalensis DC. were collected from natural habitat and authenticated by the proper plant authentication centers. The cell composition of TS slices was examined after they had been dyed and mounted. The powders of the sample drug were studied organoleptically. The Extractive values and Ash values were represented in Pie-chart form designed by using Microsoft Excel. Results: The Bark of the Mahonia nepalensis DC. is brown in color externally but the inner side is yellow with features of general anatomy of dicot stem on transverse section. The pharmacognosy and phytochemical investigations of the stem bark of Mahonia nepalensis DC shows presence of starch grain, intermedullary rays as diagnostic characters. Mayer’s reagent test and Dragon Droff’s reagent test are positive in the test signifies that the present of Alkaloids and TLC values were evaluated. Conclusion: The key diagnostic characteristics of Mahonia nepalensis DC’s stem were identified from its pharmacognosy. The stem of Mahonia nepalensis DC was authenticated and subjected to quality control using phytochemical tests and TLC, which were derived and described.

The influence of nano clay content on the strength of cement concrete

In order to clearly study the influence of nano clay content on the strength of cement concrete, superplasticiser (SP), naphthalene superplasticiser (FDN), polycarboxylic superplasticiser (PC) and aliphatic superplasticiser (SAF) are selected as test reagents, and nano clay with content of 0%, 0.5%, 1% and 1.5% and different kinds of superplasticisers are added to the water cement concrete mixture with water binder ratio of 0.36, The change of concrete cement paste mixed with nano clay is characterised by SEM test method from the micro scale, and the influence law of nano clay content on the compressive strength and flexural tensile strength of cement concrete is obtained. The results show that when the content of nano clay is 1.0%, the compressive strength of concrete reaches the maximum, and when the content of nano clay is 1.5%, the flexural tensile strength of concrete reaches the maximum.

African Swine Fever Virus (ASFV) Indirect ELISA Test Based on the Use of the Soluble Cytoplasmic Semi- purified Antigen (ASFV CP-Ag).

The present chapter describes a simple and economic indirect enzyme immunoassay (ELISA ) for African swine fever virus (ASFV) antibody detection based on the use of the soluble cytoplasmic fraction of ASFV-infected monkey stable cells (MS). The soluble antigen proteins of ASFV-infected cells are separated by sucrose precipitation centrifugation, and the supernatant above the sucrose layer is used as an ELISA antigen. The test serum sample reacts with the cytoplasmic soluble fraction, and antibodies are detected using a protein A-peroxidase conjugate. This crude antigen is currently recommended as a test reagent in screening and diagnostic tests by the World Organization for Animal Health (OIE).

Diagnostic value of occult haemolacria

To study tears for occult (latent) haemolacria in women and compare the representativeness of the main methods of tear examination. Tear samples from 72 women aged between 18 and 46 years were evaluated using two methods: microscopic and chemical with reagent test strips. In the follicular phase of the menstrual cycle, erythrocytes in tears were detected in 59.1% (13) subjects, which was significantly higher (χ2=4.2, p=0.041) compared to the luteal phase - erythrocytes were detected in 40.9% (9) women. The proportion of women in the luteal phase of the cycle who had single epithelial cells in the tear (76.9%) was significantly lower (χ2=5.2, p=0.022) than in women in the follicular phase (83.9%). The predictive value of the chemical method was 0.53 of that of the microscopic one. Microscopically, blood was detected in 30.6% of tear samples, while chemically - in 16.7%. An associative relationship was found between occult haemolacria in women and a diagnosis of endometriosis (K=0.75, p≤0.05). The proportion of women with erythrocytes in tears determined with the chemical method was 41.7% in patients with endometriosis, which was significantly higher (χ2=6.5, p=0.011) than 11.7% in women without endometriosis. Fluctuations of hormones during the menstrual cycle are reflected in tear composition, manifested as an increase in the number of epithelial cells in the luteal phase, and erythrocytes in the follicular phase. Erythrocytes found in a tear amid accumulations of epithelial cells, their glandular atypia and neutrophilic infiltration in patients with endometriosis may indicate the manifestation of extragenital endometriosis. The microscopic method turned out to be more sensitive in detecting occult haemolacria, and additionally it provided information about other cells. At the same time, the chemical method, which was relatively diagnostically informative, faster and technically simpler, can be used for screening.

CapsNet-COVID19: Lung CT image classification method based on CapsNet model.

The outbreak of the Corona Virus Disease 2019 (COVID-19) has posed a serious threat to human health and life around the world. As the number of COVID-19 cases continues to increase, many countries are facing problems such as errors in nucleic acid testing (RT-PCR), shortage of testing reagents, and lack of testing personnel. In order to solve such problems, it is necessary to propose a more accurate and efficient method as a supplement to the detection and diagnosis of COVID-19. This research uses a deep network model to classify some of the COVID-19, general pneumonia, and normal lung CT images in the 2019 Novel Coronavirus Information Database. The first level of the model uses convolutional neural networks to locate lung regions in lung CT images. The second level of the model uses the capsule network to classify and predict the segmented images. The accuracy of our method is 84.291% on the test set and 100% on the training set. Experiment shows that our classification method is suitable for medical image classification with complex background, low recognition rate, blurred boundaries and large image noise. We believe that this classification method is of great value for monitoring and controlling the growth of patients in COVID-19 infected areas.

Quality Control in the Clinical Medical Laboratory Based on Mobile Medical Edge Computing.

Today, the IT departments of many healthcare organizations are suffering from data storage and network speed. Doctors diagnose patients to help them out, and this treatment process needs to be well managed. Clinical trial data are the main basis for judging the patient's condition in the medical process. With the rapid development of clinical laboratory technology in China, important achievements have been made in medicine. Clinical hospitals use a large number of different types of testing reagents and equipment, and the accuracy of testing data has become a key issue for testing results. The accuracy of testing data comes from the zero error of clinical testing, derived from the rigor and operational rigor of the testers in clinical testing. This article is dedicated to the quality control analysis and research based on mobile medical edge computing in clinical trials. This article introduces the relevant theories of mobile medical edge computing technology and quality control methods, with reference to the study on the general medical students' clinical competency in problem-solving, communication skills, procedure, history, and physical examination and critical and non-critical indicators in objective structured clinical examination (OSCE). In addition, a routine testing group and a quality control group for strengthening important aspects of medical testing were designed to conduct comparative experiments. Experiments have proved that the quality control group for strengthening the important links of medical testing has a higher index than the routine testing group in all aspects. Among them, the detection accuracy rate can reach up to 99.48%, which is of great significance for the improvement of the detection link of the patient's condition and the follow-up diagnosis and treatment link.