Test Of Independence Research Articles

HIV, HCV and HIV-HCV Coinfections in the General Population versus Inmates from Romania.

The objective of this study was to analyze the epidemiological links of the human immunodeficiency virus (HIV), hepatitis C virus (HCV) and HIV-HCV coinfections to less studied types of transmission in certain populations. We performed an observational, prospective study on 903 patients aged between 15-87 years who took part in the Open Test Project. They were divided in two subgroups: general population vs. individuals from prisons who were questioned about multiple risk factors. A chi-square independence test was used to establish correlations between risk factors and results of screening tests. Logistic regression was used to calculate the probability of a reactive screening test based on each independent risk factor and age. HIV was very strongly associated with unprotected sexual intercourse with HIV-positive partners (the strongest association), unprotected sexual intercourse with sex workers, newly diagnosed sexually transmitted diseases (STDs), intravenous drug users (IDUs) and sharing injecting materials. In the case of HCV reactive tests, very strong associations have been established with IDUs (the strongest association), unprotected sex with IDUs and sharing injecting materials. Our study indicates the need for implementing targeted public health programs, tailored to the local epidemiology that can ultimately lead to micro-elimination of hepatitis and HIV infections in this area.

Influence of religio-cultural beliefs on whole-body donation: A quantitative analysis of a predominantly South African Pedi Community

BackgroundBody donation is a predominant mode of body sourcing in many countries including South Africa; however, it continues to be limited among the different ethnic groups in the Black African population of South Africa which have been observed to be largely unwilling to provide consent to donate their bodies. Religious and cultural beliefs have been suggested to influence willingness to donate one’s body to science, with most religions regarded as accepting of body donation globally. In contrast, cultural beliefs and practices in the African continent impede body donation. Therefore, it is crucial to understand the factors that influence willingness to participate in body donation among the various ethnic groups of the Black African population of South Africa, particularly the Pedi ethnic group. PurposeThis study sought to investigate factors that determine the disposition to participate in body donation and the influence of religious and cultural beliefs on willingness to consent to donate one’s body among a predominantly Pedi community of the Black African population of South Africa in the Limpopo province. MethodsIn this cross-sectional study, 300 four-page questionnaires were distributed to conveniently sampled individuals from the GaMaphale (Pipa) village of the Limpopo province of South Africa. Descriptive statistics (frequency tables and Pearson’s chi-square test for independence) followed by a stepwise backward regression were conducted to obtain key factors determining willingness to donate one’s body, the influence of religious and cultural beliefs, and also the likelihood of willingness to participate in body donation in this community. A p-value of < 0.05 was considered statistically significant. ResultsA total of 190 (63 %) responses were obtained. The level of awareness about body donation was 30 % (n= 57/190), and 41 % (n= 78/190) of respondents were willing to donate their bodies. Age (p = 0.001), educational level (p = 0.037), belief in a higher entity (p = 0.007), ancestral (p < 0.001), and religious (p < 0.001) beliefs allowing for body donation were statistically associated with willingness to donate one’s body. The elderly respondents were less likely to agree to donate their bodies compared to younger adults. Respondents who understood that religion allowed them to self-donate were 50 times more likely to agree to donate their bodies than those who did not. Discussion and conclusionFindings reflect that a high percentage of respondents were unaware of body donation while a considerable number of respondents were willing to donate their bodies to science based on their religious and cultural beliefs allowing them to do so. Potential body donors from this community will likely be younger and belong to religions that allow body donation. The need to increase awareness about body donation programs through awareness campaigns, especially among religious organizations that may allow for body donation, is warranted in this community.This work was supported by the National Research Foundation (TTK220214660416).

Seasonal Patterns of Injury Characteristics Among Farmers and Ranchers in the U.S. Central States

ABSTRACT Objective In high-risk agricultural environments, tasks, weather, and work conditions vary throughout the year. Also, injuries peak during certain periods. The primary objective of this study was to examine operator- and farm-level characteristics as risk factors for injuries within each of the four seasons. The secondary objective was to examine seasonal differences in the incident location and primary cause of these injuries. Method We analyzed data from the 2018 and 2020 Farm and Ranch Health and Safety Surveys (FRHSS), conducted in seven U.S. states by the Central States Center for Agricultural Safety and Health (CS-CASH), which were collected using a stratified random sampling approach to ensure representativeness. The survey data were merged with operation-level data from the Farm Market iD database. We employed Generalized Estimating Equations (GEE) to examine the association of seasonal injuries with individual and operation-level characteristics. The chi-square test of independence was used to assess the association between injury incident location and season, as well as injury cause and season. Pairwise Z-tests of proportions were conducted to evaluate the differences in the proportions of injuries due to specific combinations of injury location and cause across each pair of seasons. Results Surveys conducted in 2018 and 2020 yielded a combined response rate of 15.9%, with 5,428 responses and 7,915 unique operators. Of these, 903 operators reported at least one injury during the past 12 months. Seasonally, most injuries occurred in spring (34.2%), followed by summer (24.7%). Male operators had higher injury odds in the spring (adjusted OR = 1.42) and summer (aOR = 2.41). Those managing both a farm and a ranch reported increased injury risks in winter (aOR = 1.73) and spring (aOR = 1.48). Operators in cow-calf operations faced higher springtime injury risks (aOR = 1.45). High stress and exhaustion were consistent risk factors across all seasons. The highest proportion of injury incidents occurred in the farmyard (43.6%), and livestock were the most common cause of injury (24.9%). Conclusion Results highlight the need for season-specific prevention and intervention strategies, considering farmers’ and ranchers’ risk characteristics, injury locations, and causes. These findings can inform targeted measures for high-risk populations at optimal times and locations.

Credibility Judgments in Higher Education: A Mixed-Methods Approach to Detecting Misinformation from University Instructors

Given the convenience with which information can now be acquired, it is crucial to analyze cases of potential misinformation and disinformation in postsecondary education. Instructor credibility judgments were measured using descriptive survey research, and the main objective was to investigate trends related to misinformation, credibility, trust, bias, and others in graduate students and on a graduate program basis. Participants were surveyed from a land grant institution in the southeast United States where 186 graduate students completed an electronic survey on the detection of misinformation and similar experiences. Graduate students were divided based on graduate program into STEM (sciences, technology, engineering, and mathematics) and non-STEM groups. Quantitative methodologies included validated questionnaires developed by researchers containing Likert-type scale questions. Chi-square tests of independence and frequencies served as primary analyses. Participants in both STEM and non-STEM groups detected the following: misinformation, bias, challenges, intimidation, risk of measurable consequences, pressure to conform, and skepticism from post-secondary instructors. There were significant differences between the type of student for trust in claims (p &lt; 0.05), while the perception of potential consequences tended to be different between the types of graduate students (0.05 &lt; p &lt; 0.10). Participants in both STEM and non-STEM groups reported perception bias in science material presentation, with STEM students reporting less bias. Qualitative methodologies included optional open response boxes to provide supporting details or narratives. Reliable and validated thematic coding following served as the primary analysis. Students disciplined in STEM and non-STEM faced misinformation, bias, challenges, intimidation, risk of measurable consequences, pressure to conform, and skepticism from post-secondary instructors. Graduate students reported consistent instances of misinformation and bias about science and agriculture topics in both science and non-science-focused classrooms.

Evaluating Bereavement Response Plans in Daycare: A Comparative Analysis of Support Systems in Denmark and Norway

This study examines the implementation and use of bereavement response plans (bereavement plans) in Danish and Norwegian daycare institutions. Two large-scale surveys were conducted to explore the implementation rates, satisfaction levels, and source of the plans. The data collected was analyzed using frequency analyses and Chi-square tests of independence. The findings suggest that 87% of Danish institutions have bereavement plans compared to 98% in Norway. Furthermore, the study highlights the differences in the development of bereavement plans between the two countries, as Danish institutions trust their individually tailored plans more than their Norwegian counterparts, while the Norwegian plans seem to be of higher quality. Despite these differences, bereavement plans provide a sense of safety for staff when supporting bereaved children in both countries. This study contributes to the ongoing efforts to improve bereavement support in daycare institutions and emphasizes the importance of continued research and development in this area.

Enhanced event-based surveillance: Epidemic Intelligence from Open Sources (EIOS) during FIFA World Cup 2022 Qatar

BackgroundPublic health threats can significantly impact mass gatherings and enhancing surveillance systems would thus be crucial. Epidemic Intelligence from Open Sources (EIOS) was introduced to Qatar to complement the existing surveillance measures in preparation to the FIFA World Cup Qatar 2022 (FWC22). This study estimated the empirical probability of EIOS detecting signals of public health relevance. It also looked at the factors responsible for discerning a moderate-high risk signal during a mass gathering event. MethodsThis cross-sectional descriptive study used data collected between November 8th and December 25th, 2022, through an EIOS dashboard that filtered open-source articles using specific keywords. Triage criteria and scoring scheme were developed to capture signals and these were maintained in MS Excel. EIOS’ contribution to epidemic intelligence was assessed by the empirical probability estimation of relevant public health signals. Chi-squared tests of independence were performed to check for associations between various hazard categories and other independent variables. A multivariate logistic regression evaluated the predictors of moderate-high risk signals that required prompt action. ResultsThe probability of EIOS capturing a signal relevant to public health was estimated at 0.85 % (95 % confidence interval (CI) [0.82 %−0.88 %]) with three signals requiring a national response. The hazard category of the signal had significant association to the region of occurrence (χ2 (5, N = 2543) = 1021.6, p < .001). The hazard category also showed significant association to its detection during matchdays of the tournament (χ2 (5, N = 2543) = 11.2, p < .05). The triage criteria developed was able to discern between low and moderate-high risk signals with an acceptable discrimination (Area Under the Curve=0.79). ConclusionEIOS proved useful in the early warning of public health threats.

Postvasectomy Semen Analysis Compliance With Utilization of a Mail-In Semen Analysis Kit.

The AUA recommends postvasectomy semen analysis (PVSA) to verify successful vasectomy. However there remains poor patient compliance. We sought to assess whether mail-in PVSA improves patient compliance across a wide range of practice types. Prospective data were collected on all men who received a Fellow PVSA kit between April 2021 and August 2023 in a nationwide cohort. Date of kit activation, practice type, clinic zip code, and date of kit accession/processing at the lab was collected. Compliance rates for each practice area were reported. χ2 tests of independence, logistic regression models, and multivariable logistic analysis were performed to assess the impact of relevant variables. Overall compliance across all practice areas was 69% following an 18-week period of observation (n = 16,105) and 82% (n = 6687) following a 40-week period. Compliance rates were highest and similar for small urology practices (<5 providers), including Veterans Affairs practices, ranging from 76% to 82% at 18 weeks to 85% to 87% at 40 weeks. Large urology practices had slightly lower compliance rates with 66% at 18 weeks to 80% at 40 weeks. The univariable logistic regression model demonstrated that patients in small urology practices have a 63% greater odds of 26-week compliance, on average, compared to those who receive care in large urology practices (odds ratio 1.63, 95% CI 1.48-1.79). Fellow's mail-in PVSA offers improved PVSA compliance over previously published data. Improved compliance is seen across all practice types. Despite these successes, there is significant room for improvement to achieve 100% compliance.

Can industry 4.0 work together with lean? An investigation from Malaysian lean manufacturing sector

An examination of Industry 4.0 (I4.0) and its impact on lean manufacturing (LM) is not yet detailed, properly evaluated or validated. Notably, there is a lack of recent studies that evaluated I4.0 technology mapping and prioritization in the LM industrial context in Malaysia. The study aims to evaluate how I4.0 technology enablers influence the use of lean tools. Moreover, this study evaluates the correlation between company type and I4.0 implementation maturity, familiarity, and sources exhibiting an inclination to advance skills. The study evaluated 208 respondents belonging to several LM enterprises in Malaysia. Self-administered questionnaires were used and association analysis, based on the independence test, was used to assess the consensus based on the devised hypothesis. The outcomes were obtained using correspondence assessment of the asymptotic significant correlation coefficient (p) in which to draw conclusions using the p determined during hypothesis evaluation, a 0.05 significance level indicates all null hypotheses must be accepted. Moreover, the three elements; I4.0, LMP, and LMSs are significant with 0.897, 0.980, and 0.876 values, respectively. The outcomes indicate a strongly positive correlation between I4.0 and LM system (LMS). This observation is expected to help Malaysian LM practitioners to anticipate the future lean landscape based on the boundless innovation provided by these Technologies as the anticipated outcome is a strong positive correlation between I4.0 and LM systems (LMS). This finding would empower Malaysian LM practitioners to anticipate the future of lean manufacturing, shaped by the boundless innovation potential of I4.0 technologies. By embracing this convergence, companies can unlock a future of enhanced productivity driven by autonomous operations and data-driven decision making. Therefore, using lean in the context of I4.0 is a promising combination for improving future productivity through autonomous operations.

Risk factors of children's low birth weight and infant mortality in Bangladesh: Evidence from binary logistic regression and Cox PH models.

Low birth weight is recognized as a pivotal risk factor affecting child survival and growth. Although Bangladesh has made commendable progress in public health, an infant mortality rate of 38 per 1000 live births and a 16% prevalence of low birth weight remain significant concerns compared to other developing countries. This situation poses a significant challenge for the formulation of future health policies in Bangladesh. As a result, this study aims to identify potential risk factors contributing to low birth weight and infant mortality among children in Bangladesh. The data is extracted from the 2014 Bangladesh Demographic and Health Survey. The response variables are infant mortality and low birth weight. In the bivariate analysis, Log-rank tests and Chi-square tests of independence were conducted. Cox proportional hazards and binary logistic regression models were utilized to determine the impact of risk factors on infant mortality and low birth weight. This study identified several significant factors associated with children's low birth weight, including wealth index, parental education, birth order, twin births, mother's body mass index, and child sex. Additionally, wealth index, parental education, twin status, media exposure, birth order, antenatal care visits, prenatal care assistance, and low birth weight were identified as potential risk factors for infant mortality in Bangladesh. This study revealed that maternal and child characteristics, along with knowledge about child health care during pregnancy, can potentially reduce the risk of low birth weight and infant mortality among children in Bangladesh. To improve child health and survival, policymakers should prioritize community-based health education programs, and encourage parents to seek healthcare information from institutional medical facilities during pregnancy and after birth.

The Influence of Realistic Mathematics Education on the Mathematical Problem-Solving Ability and Learning Independence of Class V Students at Jarakan State Elementary Schools

&lt;p&gt;This research aims to describe the influence of the realistic mathematic education approach model on students' mathematical problem-solving abilities and define the impact of the realistic mathematic education approach on students' learning independence. The sample used in this research was the VA and VB classes of Jarakan State Elementary School, with 35 students in each class. This type of research is quasi-experimental research. The instruments used in this research were test questions on students' mathematical problem-solving abilities and learning independence questionnaires. The research test carried out by the researcher used a significance level of 5% so that if the significance value is less than 0.05, it can be concluded that the realistic mathematic education approach positively affects students' mathematical problem-solving abilities. The learning independence test can be seen by comparing the total score, mean value, and percentage of the level of student response contained in the descriptive statistical analysis. If the score, mean, and percentage of student response level percentage of the experimental class are higher than the control class, it can be concluded that the realistic mathematic education approach has a positive influence on student learning independence.&lt;/p&gt;

Are we all anxious about the same thing? A comparison of lay definitions of music making and performance in the context of music participation

Adverse experiences toward making music can be found in both musicians (music performance anxiety) and non-musicians (avoidance of participating in musical experiences). Both anxiety and avoidance are driven by perceptions and attributions about particular stimuli, based on schemas developed via direct and indirect experiences. Most research within music psychology focuses on the views of professional or student musicians. However, little is known about music avoidance in the general population, or their perceptions of music and performance. This article proposes to address this gap by comparing Australian adults’ perceptions toward music making and performance. A total of 116 participants self-identified as musical/non-musical, a musician/non-musician, and a performer/non-performer, then defined various terms and phrases related to music making and performance. Logistic regressions indicated that those with exposure to the music education system were significantly more likely to self-identify as musical, a musician, and a performer. Tests of independence reveal a relationship between self-identification and perceptions of music making, performance, anxiety, and willingness to engage. Possible mechanisms underlying the results of this study are discussed and a conceptual expansion related to music performance anxiety (MPA) and music making is proposed.

Digital News Audiences in Mexico: Age Segment Analysis

This study aims to determine whether news consumption, media trust, interest in news, and political affinity are correlated with the generational segment of the market, measured by the age of the digital audience. Furthermore, this study seeks to verify whether age is associated with digital audiences’ paying attention to journalists or news brands. This research is based on a survey of 2,005 people in Mexico by YouGov in 2022, commissioned by the Reuters Institute for the Study of Journalism at the University of Oxford as part of its annual Digital News Report. The statistical method used was the chi-square test of independence between qualitative variables. The results showed that age correlates with consumption habits, media trust, interest in news, and citizens’ political affinity for digital audiences in Mexico. Moreover, age relates to whether digital audiences focus on journalists or news brands in Latin America. This study delves into the characteristics of digital audiences in Mexico and contributes from a media economics, media management, and media marketing perspective. It also helps media organizations assess their market strategy, market segmentation, and target markets based on the characteristics of digital news audiences in Mexico.

The impact of pregnancy on sexual functioning in Polish women

IntroductionSexual life of pregnant women alters during pregnancy due to the physiologic,’ anatomic and hormonal changes in her body. Therefore, the aim of this study was to evaluate female sexual functioning after becoming pregnant.Patients and methodsA prospective survey study including 148 pregnant women. An anonymous questionnaire including 60 inquiries concerning intimate relationship before and during pregnancy was performed. The following statistical test were used: Chi-square test of independence, Spearman’s rank correlation coefficient and Wilcoxon test. The significance level of p = 0.05 was assumed.ResultsMost of the respondents were between 31 and 40 years old (55%). Majority of them were married (86%). During pregnancy, slightly more than half of women had a moderate need for sexual intercourse (51%), a large percentage of them had a low need (32%), a high need for intercourse was declared by 17% of women. The correlation analysis showed a statistically significant relationship between women’s education and the need for sex before pregnancy (p = 0.049). Respondents with higher degrees of education more often felt the need for intercourse before pregnancy. No correlation was found between education and the need for intercourse after pregnancy (p = 0.107). After becoming pregnant, 51% of women had less need for intercourse, 7% more, and 42% the same as before pregnancy, and these differences were statistically significant (p < 0.001). Also, a decreased satisfaction with sexual intercourse was reported during pregnancy (p < 0.001). After getting pregnant, the average number of intercourses decreased in majority (71%) of respondents, and differences in the number of intercourses before and during pregnancy were statistically significant (p < 0.001).ConclusionPregnancy has significant impact on woman’s sexuality. After becoming pregnant majority of women declare less need for sexual intercourses, decreased number of intercourses with less satisfaction.

Determinants of comprehensive knowledge of HIV transmission and prevention among women of reproductive age 15-49 years in Nigeria.

Comprehensive knowledge of HIV transmission and prevention has been reported as a necessary factor for ending the HIV epidemic. Women of reproductive age identified as a vulnerable group to HIV infection require this knowledge to prevent contracting HIV infection. Therefore, this study aimed to identify those factors that impact these women's comprehensive knowledge of HIV transmission and prevention. This study used secondary data from the 2018 Nigerian Demographic and Health Survey. A weighted sample of women of reproductive age with complete data on the determinants and comprehensive knowledge of HIV was included in each analysis. The Chi-square test of Independence was used to determine the association between the determinants and the dependent variable. Bivariable and multivariable logistic regression analysis was done to predict the effects of the determinants on the comprehensive knowledge of women of reproductive age. Variables with a p-value of ≤ .01 were considered statistically significant determinants of comprehensive knowledge of HIV transmission and prevention. The findings showed that women of reproductive age with no or lower level of education, living in rural areas, poor wealth index, do not listen or listen less frequently to radio, and watch television less frequently were more likely to have comprehensive knowledge of HIV transmission and prevention at Alpha = .01. The number of women with comprehensive knowledge of HIV was low compared with those with no comprehensive knowledge. This is a concern that needs to be addressed. Further studies using primary data to validate the findings of this study that individuals with no or lower level of education, living in rural areas, poorer or middle wealth index, do not listen or listen less frequently to radio and do not watch or watch less frequently television were more likely to have comprehensive knowledge of HIV transmission and prevention are recommended.

Etiology of bloodstream infections among pediatric patients in Bauchi metropolis, north-east Nigeria

Background: The prevalence of bacterial infections in children is increasing by the day, this gives a cause for concern regarding the high rate of bloodstream infection (BSI) and associated mortality in developing countries. Physical signs and symptoms may be useful but have limited specificity which would be addressed with bacteriologic blood culture. This study was aimed at determining the etiologic agents of bacteremia and the prevalence in pediatric patients aged 0-5years. Methods: Two hundred and sixty- two (262) blood samples were analyzed from children aged 0-5years, attending hospitals within Bauchi metropolis with features suggestive of septicemia. Samples were collected under aseptic conditions and automated blood culture was done on BacT/ALERT 3D Biomerieux, Germany. Positive blood culture was sub-cultured on blood agar and MacConkey agar and identified on Vitek2 (bioMѐrieux, France) automated system after gram staining. Changes in pathogen distribution according to age and gender were determined by the chi-square test and statistical significance confirmed if a two-tailed p-value was not more than 0.01. Results: Bacteria was isolated in 119 (45.4) of samples with highest rate among neonates 54(45.3). Staphylococcus aureus 21 (17.6) was the most predominant organism isolated across all age groups with a higher occurrence in patients&lt;1year old. A chi-square test of independence for culture positivity and age/gender at p&lt;.01 was not significant Conclusion: In this study both Gram-positive and Gram-negative bacteria are implicated in pediatric bloodstream infections. Staphylococcus aureus was the most predominant organism followed by members of the Enterobacterales (Enterobacter spp, Klebsiella spp and Escherichia coli).

Diet quality in medical trainees: a cross-sectional assessment comparing medical students and primary care residents using the Rapid Eating Assessment for Participants—shortened version

BackgroundThe diet quality of the US population is significantly unhealthy, with critical long-term implications for the nation’s health. A few studies have explored diet quality in the future primary care workforce. This cross-sectional study quantifies the diet quality of medical students and primary care residents at a Midwestern college of medicine in the United States.Methods250 medical students and 148 primary care residents were surveyed electronically utilizing the Rapid Eating Assessment for Participants—Shortened Version (REAP-S). The survey consists of 13 questions that can be scored on a scale from 1 to 3, along with 3 questions that are not scored. The average REAP-S score for a US omnivorous diet is 32 (range 13 to 39) with higher scores indicating a higher quality diet. We obtained average REAP-S scores for all respondents and descriptive summary statistics for individual REAP-S items. Students’ REAP-S total scores were compared to those of residents and the interaction between student/resident status and BMI category on REAP-S total scores were analyzed using analyses of variance. Differences between students and residents on BMI categories and other outcomes (individual REAP-S items, pandemic dietary and weight changes) were analyzed using Chi-Square Tests of Independence or Fisher’s Exact Tests.ResultsMedical students (n = 99; 39.6% response rate) had a significantly higher mean REAP-S score (30.5, SD = 3.9) than primary care residents (n = 72; 49% response rate) (mean = 28.6, SD = 3.9; p = 0.006). Total mean REAP-S scores were significantly higher for those with BMI < 25 (mean = 30.8, SD = 3.7) than for those with BMI > = 25 (mean = 28.3, SD = 4.0; p < 0.001). There was not a statistically significant interaction between role (student vs. resident) and BMI category on total REAP-S scores (p = 0.39). Most respondents (89.3%) indicated that they were willing to improve their diet.ConclusionOur data suggest that the diet quality of surveyed medical students and primary care residents, as quantified by the REAP-S, is suboptimal. Early detection and improvement of diet quality may be necessary for our medical profession trainees to avert potential long-term adverse cardiometabolic health outcomes, and to optimize the health of our future primary care workforce.

Computational Test for Conditional Independence

Conditional Independence (CI) testing is fundamental in statistical analysis. For example, CI testing helps validate causal graphs or longitudinal data analysis with repeated measures in causal inference. CI testing is difficult, especially when testing involves categorical variables conditioned on a mixture of continuous and categorical variables. Current parametric and non-parametric testing methods are designed for continuous variables and can quickly fall short in the categorical case. This paper presents a computational approach for CI testing suited for categorical data types, which we call computational conditional independence (CCI) testing. The test procedure is based on permutation and combines machine learning prediction algorithms and Monte Carlo cross-validation. We evaluated the approach through simulation studies and assessed the performance against alternative methods: the generalized covariance measure test, the kernel conditional independence test, and testing with multinomial regression. We find that the computational approach to testing has utility over the alternative methods, achieving better control over type I error rates. We hope this work can expand the toolkit for CI testing for practitioners and researchers.

Prevalence, distribution, and inequitable co-occurrence of mental ill-health and substance use among gender and sexuality diverse young people in Australia: epidemiological findings from a population-based cohort study

PurposeTo estimate the prevalence, distribution, and co-occurrence of mental ill-health and substance use among gender and sexuality diverse young people relative to their cisgender and heterosexual peers in Australia using population-level, nationally representative data.MethodsWe utilised Wave 8 (2018) data from the Longitudinal Study of Australian Children (N = 3037, Mage = 18.4) collected via an assessment protocol comprising interviews, direct observations, and assessments (on average 60 min per survey occasion). Weighted prevalence ratios and logistic regression models adjusted for demographic confounders were used to estimate the prevalence and distribution of mental ill-health (psychological distress, past 12-month self-harm thoughts and behaviours, past 12-month suicidal ideation, planning, attempt/s) and substance use outcomes (past 12-month cigarette, alcohol, and marijuana use) across gender identity (trans vs. cisgender), sexuality (gay/lesbian, bisexual, queer [those identifying with an ‘other’ sexuality identity that is not ‘gay’, ‘lesbian’, ‘bisexual’, or ‘heterosexual’] vs. heterosexual) and sexuality diversity status (sexuality diverse vs heterosexual) subgroups. Sex-stratified prevalence rates and accompanying adjusted logistic regression models were also used to assess mental ill-health and substance use disparities by sexuality diversity status. Adjusted multinominal logistic regression models were used to test disparities in co-occurring outcomes by sexuality identity) sexuality status sub-groups, and Fisher’s Exact Test of Independence for co-occurring disparities by gender identity (due to small sample size). All analyses used Wave 8 sample weights and adjusted for postcode-level clustering.ResultsAmong gender and sexuality diverse participants, 59 − 64% reported high or very high levels of psychological distress, 28 − 46% reported past 12-month self-harm ideation or attempts, and 26 − 46% reported past 12-month suicidal ideation, planning, or behaviour. We found significant disparities in high/very high levels of psychological distress, self-harm behaviours and suicidal behaviours among trans participants (adjusted odds ratios (aORs) ranged from 3.5 to 5.5) and sexuality diverse participants (aORs ranged from 3.5 to 3.9), compared with cisgender and heterosexual participants, respectively. Highest disparities in any past 12-month self-harm and suicidal behaviours appeared most pronounced among trans participants and queer participants compared with their cisgender, heterosexual counterparts. Minor differences by sex among sexuality diverse participants were observed for select mental ill-health outcomes. Sexuality diverse participants, and particularly sexuality diverse females, were significantly more likely to report past 12-month cigarette use and past 12-month marijuana use (adjusted odds ratio (aORs) ranging 1.4–1.6). Trans young people were at significantly elevated risk of mental ill-health in co-occurrence with cigarette and marijuana use compared with their cisgender peers (Fisher’s Exact Test of Independence p < 0.05 for all), whereas sexuality diverse young people were at greater risk of co-occurring mental ill-health and cigarette co-use and marijuana co-use, compared with their non-sexuality diverse peers (adjusted multinomial odds ratios (aMORs) ranging 2.2-6.0).ConclusionMental ill-health, substance use, and their co-occurrence disproportionately affects gender and sexuality diverse young people in Australia. Further research should study the longitudinal development of these disparities through adolescence, with close attention to the social, embodied contexts of substance use among LGBTQ + young people with the view to building LGBTQ + affirming models of harm reduction.

Construction of pan-cancer regulatory networks based on causal inference

The pan-cancer initiative aims to study the origin patterns of cancer cell, the processes of carcinogenesis, and the signaling pathways from a perspective that spans across different types of cancer. The construction of the pan-cancer related gene regulatory network is helpful to excavate the commonalities in regulatory relationships among different types of cancers. It also aids in understanding the mechanisms behind cancer occurrence and development, which is of great scientific significance for cancer prevention and treatment. In light of the high dimension and large sample size of pan-cancer omics data, a causal pan-cancer gene regulation network inference algorithm based on stochastic complexity is proposed. With the network construction strategy of local first and then global, the stochastic complexity is used in the conditional independence test and causal direction inference for the candidate adjacent node set of the target nodes. This approach aims to decrease the time complexity and error rate of causal network learning. By applying this algorithm to the sample data of seven types of cancers in the TCGA database, including breast cancer, lung adenocarcinoma, and so on, the pan-cancer related causal regulatory networks are constructed, and their biological significance is verified. The experimental results show that this algorithm can eliminate the redundant regulatory relationships effectively and infer the pan-cancer regulatory network more accurately (https://github.com/LindeEugen/CNI-SC).

Understanding Antimicrobial Prescription Practices: Insights from Small Animal Veterinarians in North Macedonia

Abstract Antimicrobial resistance (AMR) is an increasing global concern in small animal veterinary practices. The overuse and misuse of antimicrobials in companion animals can contribute to the spread of AMR between animals and humans. This study aimed to assess small animal veterinarians’ knowledge, attitudes, and behaviors towards antimicrobial use (AMU) and AMR in North Macedonia. A web-based questionnaire was developed, including questions addressing demographics, owner influence on antibiotic prescription and prescribing practices. The response rate of the small animal veterinarians was 47.50% (57/120), with most of them (84.20%) having 1-15 years of work experience. Pearson’s chi-square test of independence was used for contingency tables that met the chi-square assumption, and Fisher’s exact test was conducted for contingency tables that did not. Most veterinarians responded that they had perceived pressure and direct demands from pet owners to prescribe antimicrobials (35.09% frequently, 45.61% occasionally). In the survey, 50.77% of the veterinarians reported frequently administering antibiotics without conducting bacteriological or antimicrobial susceptibility testing. They typically resorted to these tests in cases with recurrent infections, such as skin infections (19.63%), or when empirical therapy is ineffective (14.72%). Based on the findings, it can be concluded that veterinarians play a crucial role in tackling the AMR in North Macedonia. The lack of stewardship programs or guidelines for responsible antimicrobial use in companion animals is a critical issue that requires urgent attention, emphasizing the vital role of veterinarians in combating AMR.