using ex vivo 3 Tesla or 7 Tesla magnetic field strengths from fresh prostatectomy specimens (n1⁄415) obtained from either organ donor or PCa patients under Institutional Review Board approval. To achieve the closest correspondence between histopathological components and MRI images in terms of resolution and sectioning planes, multiple high resolution imaging protocols (ranging from few minutes to overnight) were tested. Ductograms were generated as part of image post-processing. Specimens were subsequently submitted for histopathological evaluation. RESULTS: A total of 7 imaging protocols were tested (Table 1). Ex vivo 7 Tesla MRI identified normal components of prostate glands including ducts, blood vessels, concretions, and stroma at a spatial resolution of 60 X 60 X 60 mm3 to 107 X 107 X 500 mm3. A 7 Tesla spin echo image from a whole gland at a spatial resolution of 100 X 107 X 750 mm3 shown that the visible structures clearly approached the microscopic scale highlighting the microanatomy of intraprostatic tissue (Figure 1). Malignant glands and nests of tumor cells identified at 60 X 60 X 90 mm3 were highly comparable to low magnification (x2) histopathology. Ductograms enhanced the differentiation between benign and malignant glands. CONCLUSIONS: We demonstrated that critical histopathological features of the prostate gland can be identified with high resolution ex vivo MRI examination and offer promise that MR microscopy of PCa will ultimately be possible in vivo. Source of Funding: None