In view of the distinctive characteristics of satellite communication, the physical random access signals used in the terrestrial mobile communication system have to be modified or redesigned for the satellite communication system. In this paper, we boost the random access signal energy by repeating the short Zadoff–Chu (ZC) sequence based preamble signal used in the terrestrial system. Different long ZC sequences are used to scramble this cascaded sequence to distinguish different random access signals for multiple random access user equipments. For correlation performance optimization, properties of the roots for both the short and long ZC sequences are mathematically analyzed and derived. Finally, we illustrate how to construct a root set for these different long ZC sequences based on the obtained propositions in a practical way. This analytical framework provides a useful insight into ZC sequence-based random access signal design and performance analysis in mobile satellite communication systems.