Terrestrial Environments Research Articles

Georeferencing Strategies in Very Shallow Waters: A Novel GCPs Survey Approach for UCH Photogrammetric Documentation

The growing interest of the scientific community in surveying and monitoring submerged assets is motivated by the increasing demand for high-resolution products with certified accuracies. While many instrumental and methodological solutions for documenting, monitoring, and studying archaeological and cultural heritage through geomatics techniques are already available for the terrestrial environment, the challenge remains open to the underwater context. High-resolution capability and accurate positioning are still difficult to achieve in these environments. This paper discusses the limitations of positioning and georeferencing techniques in the underwater environment. It explores how existing methods and new instruments can be used to perform accurate topographic surveys of ground control points (GCPs) in very shallow waters (within 5 m depths), which can support the photogrammetric reconstruction of underwater assets. This research presents two innovative prototypes: a self-built plastic marker for topographic use in the underwater environment and a self-built aluminum pole for topographic use in the marine environment. The prototypes are tested and validated with a tilt-compensating smart antenna to reduce planar and altimetric errors when the pole is not perfectly level and to work independently of the shore proximity required when using a total station to perform said measurements.

Optimizing terrestrial locomotion of undulating-fin amphibious robots: Asynchronous control and phase-difference optimization

This paper presents an asynchronous motion-control approach for undulating-fin amphibious robots, with emphasis on optimizing the phase differences of bilateral fin-surface waves to mitigate the pitch-angle instability typically observed in synchronous control. A comprehensive ground dynamics model is used in this study, which facilitates the meticulous analysis of pitch-angle variations. Using simulation software, a virtual prototype is developed to validate the control stability. This approach is further substantiated via experimental tests conducted on a physical prototype in diverse terrestrial environments. Simulation and experimental results indicate that asynchronous motion control methods significantly enhancing postural stability undulating-fin robots during terrestrial locomotion. Experimental findings demonstrate that adopting asynchronous motion control reduces the maximum range of pitch angles by 38.9%–42.3%. This method significantly enhancing postural stability of robot terrestrial locomotion. This study provides new insights for the design of undulating-fin amphibious robots.

Functional characterization of plant specific Indeterminate Domain (IDD) transcription factors in tomato (Solanum lycopersicum L.)

Plant-specific transcription factors (TFs) are responsible for regulating the genes involved in the development of plant-specific organs and response systems for adaptation to terrestrial environments. This includes the development of efficient water transport systems, efficient reproductive organs, and the ability to withstand the effects of terrestrial factors, such as UV radiation, temperature fluctuations, and soil-related stress factors, and evolutionary advantages over land predators. In rice and Arabidopsis, INDETERMINATE DOMAIN (IDD) TFs are plant-specific TFs with crucial functions, such as development, reproduction, and stress response. However, in tomatoes, IDD TFs remain uncharacterized. Here, we examined the presence, distribution, structure, characteristics, and expression patterns of SlIDDs. Database searches, multiple alignments, and motif alignments suggested that 24 TFs were related to Arabidopsis IDDs. 18 IDDs had two characteristic C2H2 domains and two C2HC domains in their coding regions. Expression analyses suggest that some IDDs exhibit multi-stress responsive properties and can respond to specific stress conditions, while others can respond to multiple stress conditions in shoots and roots, either in a tissue-specific or universal manner. Moreover, co-expression database analyses suggested potential interaction partners within IDD family and other proteins. This study functionally characterized SlIDDs, which can be studied using molecular and bioinformatics methods for crop improvement.

A detailed 3D MRI brain atlas of the African lungfish Protopterus annectens

The study of the brain by magnetic resonance imaging (MRI) in evolutionary analyses is still in its incipient stage, however, it is particularly useful as it allows us to analyze detailed anatomical images and compare brains of rare or otherwise inaccessible species, evolutionarily contextualizing possible differences, while at the same time being non-invasive. A good example is the lungfishes, sarcopterygians that are the closest living relatives of tetrapods and thus have an interesting phylogenetic position in the evolutionary conquest of the terrestrial environment. In the present study, we have developed a three-dimensional representation of the brain of the lungfish Protopterus annectens together with a rostrocaudal anatomical atlas. This methodological approach provides a clear delineation of the major brain subdivisions of this model and allows to measure both brain and ventricular volumes. Our results confirm that lungfish show neuroanatomical patterns reminiscent of those of extant basal sarcopterygians, with an evaginated telencephalon, and distinctive characters like a small optic tectum. These and additional characters uncover lungfish as a remarkable model to understand the origins of tetrapod diversity, indicating that their brain may contain significant clues to the characters of the brain of ancestral tetrapods.

Designing payload and spaceflight operations for plants from extreme terrestrial environments

Terrestrial plants from the very limits of life are likely to harbor genes that confer an advantage in human space exploration. These plants are seemingly capable of performing mission critical functions in spaceflight and on extraterrestrial farms while informing directed gene manipulation in target plant species. However, their adaptations to physiologically extreme habitats may hinder the efficacy of routine laboratory techniques for model plants. We here present the development of Antarctic moss Ceratodon purpureus payload and flight operations for the ANT1 Radiation Tolerance Experiment with Moss in Orbit on the Space Station (ARTEMOSS) experiment to the International Space Station (ISS) given limited physical space and crew time. We demonstrate that the hydrophobic surface of Antarctic moss impedes chemical tissue fixation and precludes the use of RNAlater coupled with payload hardware deployed in standard plant spaceflight experiments. We show that deep-freezing the moss tissue on Petri plates provides adequate tissue fixation and allows the extraction of high-quality RNA suitable for gene expression profiling. We replaced hardware with stacks of Petri plates housing Antarctic moss and chemical fixation with deep-freezing in a cryogenic GLACIER freezer. Our design can be translated to other plant species to expand current experimentation techniques with plants from extreme terrestrial environments in order to advance human space exploration.

EXPERIMENTAL RESEARCH ON THE PRESENCE OF HYDROCARBONS IN THE SOIL OF THE NORTH CONSTANTA ZONE

This experimental research engages in an investigation of sediment quality, with a focus on the identification and quantification of hydrocarbons and other contaminants that may adversely affect the health of the marine and terrestrial ecosystem in the Black Sea region. The purpose of this experimental research is to contribute to the understanding of the dynamics of hydrocarbon pollution and its impact on the marine and terrestrial environment in the Black Sea area.

Clumped isotopes reveal relationship between mussel growth and river discharge

Freshwater mussels preserve valuable information about hydrology, climate, and population dynamics, but developing seasonal chronologies can be problematic. Using clumped isotope thermometry, we produced high-resolution reconstructions of modern and historic (~ 1900) temperatures and δ18Owater from mussel shells collected from an impounded river, the Brazos in Texas, before and after damming. We also performed high-resolution growth band analyses to investigate relationships between mussel growth rate, rainfall, and seasonal temperature. Reconstructed δ18Owater and temperature vary little between the modern (3R5) and historic shell (H3R). However, a positive relationship between reconstructed δ18Owater and growth rate in H3R indicates that aside from diminished growth in winter, precipitation and flow rate are the strongest controls on mussel growth in both modern and pre-dam times. Overall, our results demonstrate (1) the impact, both positive and negative, of environmental factors such as flow alteration and temperature on mussel growth and (2) the potential for clumped isotopes in freshwater mussels as a paleohydrology and paleoclimate proxies in terrestrial environments.

Genome-resolved metagenomics identifies novel active microbes in biogeochemical cycling within methanol-enriched soil.

Metagenome assembled genomes (MAGs), generated from sequenced 13C-labelled DNA from 13C-methanol enriched soils, were binned using an ensemble approach. This method produced a significantly larger number of higher-quality MAGs compared to direct binning approaches. These MAGs represent both the primary methanol utilizers and the secondary utilizers labelled via cross-feeding and predation on the labelled methylotrophs, including numerous uncultivated taxa. Analysis of these MAGs enabled the identification of multiple metabolic pathways within these active taxa that have climatic relevance relating to nitrogen, sulfur and trace gas metabolism. This includes denitrification, dissimilatory nitrate reduction to ammonium, ammonia oxidation and metabolism of organic sulfur species. The binning of viral sequence data also yielded extensive viral MAGs, identifying active viral replication by both lytic and lysogenic phages within the methanol-enriched soils. These MAGs represent a valuable resource for characterizing biogeochemical cycling within terrestrial environments.

Microplastic pollution in terrestrial environment: Identification, characterization, and risk assessment in Indore, Central India

AbstractWidespread use of plastics and improper management of plastic waste have resulted in generation of microplastic particles in the environment. These microplastic particles are pervasive contaminants having potential to invade almost all the environmental matrices and biotic species including humans. Therefore, immediate concern is necessary to investigate the level of contamination and probable risk imposed because of microplastics. Present investigation has been carried out for estimating the presence of microplastics in terrestrial environment of Indore, which is considered as commercial capital of the state of Madhya Pradesh (India). Soil samples (30 in no.) were collected from agricultural and recreational areas of the city and analysed for the presence of microplastics. Approximately 11 particles/kg and 68.25 particles/kg were found in the agricultural and recreational site soil samples, respectively. Chemical composition analysis revealed that most of the microplastic particles belonged to polyethylene terephthalate, polypropylene, polyethylene, polyester, and polyamide. Significant presence of various fibres made up of polyester, polyacrylic, and cellulose acetate was also seen, which could be attributed to the presence of textile industries in and around Indore. Ecological risk assessment aided in concluding that study area soil is under the ‘very low’ risk category; however, considering the long‐term impacts of microplastics, suitable control measures for plastic waste are needed to be adopted.

An inexpensive and lightweight dynamic cone penetrometer for the characterization of shallow submerged sediments

Abstract Sediment compaction is an indicator of the health of benthic communities. At the same time, many of the extractive or cultivation activities on the coast take place in areas of special environmental interest and remove or compact the sediment by their own activity or simply by access on foot. Measurements of compaction or penetration resistance of marine or river sediments are not common, especially in submerged environments. Although there are devices, such as vane testers, that can be used in non‐submerged sediments, lightweight and inexpensive devices for measuring compaction in submerged sediments are rare. An apparatus for measuring sediment penetration resistance based on the dynamic cone penetrometers used in terrestrial environments is described and tested. The device is lightweight and inexpensive, and although it is intended for use in submerged sediments, it can also be used manually in sediments accessible by foot. In the process of testing the apparatus, the penetration resistance values of surfaced sediments have been compared with those obtained with a vane tester to estimate compaction. This new lightweight penetrometer provides inexpensive penetration resistance values as a measure of compaction in the sediment layer of greatest interest for the management of habitats and communities and makes it possible to establish criteria for action and to measure its effectiveness.

The Coprecipitation of Akaganeite and Jarosite and Its Implications for the Late‐Stage Diagenetic Fluids in the Vera Rubin Ridge, Gale Crater, Mars

AbstractAkaganeite and jarosite were detected in two mudstone drill samples from Vera Rubin ridge (VRR), Gale crater by Chemistry & Mineralogy X‐Ray Diffraction (CheMin). The co‐occurrence of these two minerals is quite rare in both terrestrial and Martian aqueous environments. In order to confine the chemical conditions of paragenetic akaganeite and jarosite, and provide insight into late‐stage diagenetic alterations at VRR, we synthesized akaganeite and jarosite with varying SO42− concentrations and initial pH levels. Synthetic samples were characterized using Field Emission Scanning Electron Microscopy, X‐ray powder diffraction and Raman spectroscopy. Our study reveals that akaganeite and jarosite exist in equilibrium in the solution with 0.011–0.028 M SO42− with respect to 0.6 M Cl− and an initial pH of 1.3–2.2. In combination with the CheMin detection results, the chemistry and pH values of the fluids at VRR can be further constrained. Considering the absence of goethite and the relative higher portion of akaganeite than jarosite in the drill samples, the pH values should be 1.4–2 and the S/Cl molar ratio should be within the range of 0.018–0.042. Based on our laboratory results, we hypothesize that the presence of akaganeite and jarosite at VRR represents an individual episode of acidic groundwater activity. During the late‐stage diagenetic process at VRR, upwelled acidic groundwater dissolved the local chlorides to form the Cl−‐dominated fluids. Subsequent evaporation further concentrated the acid saline fluids and therefore resulted in an extremely acidic environment (1.4 ≤ pH＜2 with S/Cl molar ratio of 0.018–0.042), which produced akaganeite and jarosite.

Phyto-Morphological and Phytochemical Insights: A Comprehensive Study of Hygrophila triflora (Roxb.) Fosb. and Sachet Leaves

Hygrophila triflora (Roxb.) Fosb. and Sachet, a notable member of the Acanthaceae family, is a herbaceous plant indigenous to South Asia, commonly found along watercourses. This study delves into the taxonomic history, morphological diversity, ecological relevance, and physicochemical properties of Hygrophila triflora. Morphologically, the plant displays dimorphism in its leaves, with adaptations suited for both aquatic and terrestrial environments. Leaves were collected from Baruipur, West Bengal, and authenticated, followed by a series of processing and microscopic analyses to explore the plant's anatomical features. The physicochemical evaluations highlighted properties such as moisture content, ash values, and extractive values, establishing a foundation for the plant's quality assessment. High-Performance Thin Layer Chromatography (HPTLC) was employed to derive a comprehensive phytochemical profile, revealing distinct Rf values when observed at two wavelengths, 254 nm and 366 nm. Additionally, a qualitative analysis showcased the presence of various secondary metabolites, such as alkaloids and flavonoids, in different extracts of the plant. These findings contribute valuable insights into the anatomical, chemical, and potential therapeutic attributes of Hygrophila triflora, paving the way for deeper pharmacological investigations and potential applications in traditional and modern medicine. This research underscores the importance of continued exploration of indigenous plant species, elucidating their roles in ecosystems and potential contributions to scientific and therapeutic domains.

Heavy metal accumulation on microplastics in compost - The role of biofilm

Microplastics, small plastic pieces ( microplastics washed with distilled water > compost free from microplastics > chemically separated microplastic > new macro-plastic, with some exceptions. Heavy metal concentrations were significantly higher in samples from Kochi, due to a larger bio-film formation on the plastics as determined by TOC and SEM analyses. The findings of the study highlight the importance of source segregation and prevention of mixing organic waste with plastic waste in MSW, to avoid heavy metal transport in terrestrial environment through microplastics.

Sulfate-reducing bacteria unearthed: ecological functions of the diverse prokaryotic group in terrestrial environments.

Sulfate-reducing prokaryotes (SRPs) are essential microorganisms that play crucial roles in various ecological processes. Even though SRPs have been studied for over a century, there are still gaps in our understanding of their biology. In the past two decades, a significant amount of data on SRP ecology has been accumulated. This review aims to consolidate that information, focusing on SRPs in soils, their relation to the rare biosphere, uncultured sulfate reducers, and their interactions with other organisms in terrestrial ecosystems. SRPs in soils form part of the rare biosphere and contribute to various processes as a low-density population. The data reveal a diverse range of sulfate-reducing taxa intricately involved in terrestrial carbon and sulfur cycles. While some taxa like Desulfitobacterium and Desulfosporosinus are well studied, others are more enigmatic. For example, members of the Acidobacteriota phylum appear to hold significant importance for the terrestrial sulfur cycle. Many aspects of SRP ecology remain mysterious, including sulfate reduction in different bacterial phyla, interactions with bacteria and fungi in soils, and the existence of soil sulfate-reducing archaea. Utilizing metagenomic, metatranscriptomic, and culture-dependent approaches will help uncover the diversity, functional potential, and adaptations of SRPs in the global environment.

Ancient DNA and osteological analyses of a unique paleo-archive reveal Early Holocene faunal expansion into the Scandinavian Arctic.

Paleo-archives are essential for our understanding of species responses to climate warming, yet such archives are extremely rare in the Arctic. Here, we combine morphological analyses and bulk-bone metabarcoding to investigate a unique chronology of bone deposits sealed in the high-latitude Storsteinhola cave system (68°50' N 16°22' E) in Norway. This deposit dates to a period of climate warming from the end of the Late Glacial [~13 thousand calibrated years before the present (ka cal B.P.)] to the Holocene thermal maximum (~5.6 ka cal B.P.). Paleogenetic analyses allow us to exploit the 1000s of morphologically unidentifiable bone fragments resulting in a high-resolution sequence with 40 different taxa, including species not previously found here. Our record reveals borealization in both the marine and terrestrial environments above the Arctic Circle as a naturally recurring phenomenon in past periods of warming, providing fundamental insights into the ecosystem-wide responses that are ongoing today.

Manganese and soil organic carbon stability on a Hawaiian grassland rainfall gradient

Manganese (Mn) is a possibly critical yet poorly understood element controlling soil carbon (C) stocks. In temperate forests, Mn availability correlates strongly with organic C decay, but we know little about its role in soil organic matter decomposition in most terrestrial environments. In this study, we evaluate Mn in grassland C dynamics along a rainfall gradient in Hawaii. We measured Mn, organic matter, and microbial enzyme activities along the rainfall gradient to evaluate relationships among Mn oxidation state, chemical/biological reactivity, and soil C turnover. Neither Mn abundance nor its oxidation state are strong predictors of organic C instability along the grassland gradient. We also used an incubation experiment to investigate how dissolved organic C and CO2 release from the grassland soil respond to increased Mn bioavailability. We found that Mn availability did not correlate with soil C instability; Mn additions corresponded with lower dissolved organic C and CO2 fluxes from soils than did additions of deionized water. Mn availability may not predict soil C stability as well as previously thought.

Prey capture kinematics of horned frogs (Anura: Ceratophryidae).

Horned frogs, members of the Ceratophryidae family, encompass a group of anurans varying in size and behavior, yet unified by morphological and behavioral traits enabling them to adopt a megalophagous diet (i.e., large prey feeding). Although the group has been the focus of numerous studies, our understanding of its feeding behavior remains limited. In this study, we characterize the feeding mechanism in five species representing the three extant genera of ceratophryid anurans, both in terrestrial and aquatic environments. We also explore the ability of Chacophrys pierottii to adjust feeding behavior based on prey type. Our findings show that all species are capable of widemouth opening, displaying an asymmetric feeding cycle. While tongue usage is the primary method for capturing prey on land, ceratophryids may use their forelimbs to manipulate prey into their mouths, exhibiting different behavioral patterns. C. pierottii shows modulation of its feeding kinematics and is also capable of some modulation of its feeding in response to prey properties.

Seasonality, rather than estuarine gradient or particle suspension/sinking dynamics, determines estuarine carbon distributions

Estuaries are important components of the global carbon cycle; exchanging carbon between aquatic, atmospheric, and terrestrial environments, representing important loci for blue carbon storage and greenhouse gas emissions. However, how estuarine gradients affect sinking/suspended particles, and dissolved organic matter dynamic interactions remains unexplored. We fractionated suspended/sinking particles to assess and characterise carbon fate differences. We investigated bacterial colonisation (SYBR Green I) and exopolymer concentrations (TEP/CSP) with microscopy staining techniques. C/H/N and dry weight analysis identified particle composition differences. Meanwhile, nutrient and carbon analysis, and excitation and emission matrix evaluations with a subsequent parallel factor (PARAFAC) analysis characterised dissolved organic matter.The lack of clear salinity driven patterns in our study are presumably due to strong mixing forces and high particle heterogeneity along the estuary, with only density differences between suspended and sinking particles. Elbe estuary particles' organic portion is made up of marine-like (sinking) and terrestrial-like (suspended) signatures. Salinity did not have a significant role in microbial degradation and carbon composition, although brackish estuary portions were more biologically active. Indicative of increased degradation rates, leading to decreased greenhouse gas emissions, which are especially relevant for estuaries, with their disproportionate greenhouse gas emissions. Bacterial colonisation decreased seawards, indicative of decreased degradation, and shifts in microbial community composition and functions.Our findings span diverse strands of research, concerning steady carbon contributions from both marine and terrestrial sources, carbon aromaticity, humification index, and bioavailability. Their integration highlights the importance of the Elbe estuary as a model system, providing robust information for future policy decisions affecting dissolved and particulate matter dynamics within the Elbe Estuary.

Impact of permafrost degradation on the extreme increase of dissolved iron concentration in the Amur river during 1995–1997

Primary production in the Sea of Okhotsk is largely supported by dissolved iron (dFe) transported by the Amur river, indicating the importance of dFe discharge from terrestrial environments. However, little is known about the mechanisms of dFe discharge into the Amur river, especially in terms of long-term change in dFe concentration. In the Amur river, extreme increase in dFe concentration was observed between 1995 and 1997, the cause of which remains unclear. As a cause of this iron anomaly, we considered the impact of permafrost degradation. To link the permafrost degradation to long-term variation in dFe concentration, we examined the changes in annual air temperature (Ta), accumulated temperature (AT), and net precipitation for three regions (northeast, south, and northwest) of the basin between 1960 and 2006. Ta and AT were relatively high in one out of every few years, and were especially high during 1988–1990 continuously. Net precipitation in late summer (July to September) has increased since 1977 and has stayed positive until 2006 throughout the basin. Most importantly, we found significant correlations between Ta and late summer dFe concentration with a 7-year lag (r = 0.54–0.69, p < 0.01), which indicate a close relationship between high Ta in year Y and increased late summer dFe concentration in year Y + 7. This correlation was the strongest in northeastern Amur basin where permafrost coverage is the highest. Similar 7-year lag correlation was also found between AT in the northeastern basin and late summer dFe concentration (r = 0.51, p < 0.01). Based on our findings, we propose the following hypothesis as a cause of iron anomaly. (1) Increased net precipitation since 1977 has increased soil moisture, which created suitable conditions for microbial dFe generation; (2) permafrost degradation during the warm years of 1988–1990 promoted iron bioavailability and led to the intensive dFe generation in the deeper part of the active layer; and (3) dFe took approximately 7 years to reach the rivers and extremely increased dFe concentration during 1995–1997. This is the first study to suggest the time-lagged impact of permafrost degradation on iron biogeochemistry in the Amur river basin.

Assessment and Sustainable Management Strategies for Plastic Waste in Can Tho City, Vietnam: A Circular Economy Approach

This study presents a comprehensive analysis of plastic waste accumulation in both terrestrial and aquatic environments in Can Tho city, Vietnam, a nation with high per capita plastic consumption and significant plastic waste discharge. Focusing on urban residential areas, riverside communities, suburbs, and rural regions, the research investigates the extent and impact of plastic waste in these diverse settings. Additionally, the study examines the accumulation of plastics at barriers, under bridges, and in the Hau River, identifying the predominance of single-use plastics and their environmental implications. The key findings indicate that the plastic waste leakage at land-based-source emission sites is substantial, with waste persisting for extended periods without effective clean-up. The study reveals a significant accumulation of plastics at barriers and bridge bases in aquatic environments, including along the river. The pollution level was observed to be more influenced by the quantity of waste rather than its mass per unit area, emphasizing the need for targeted waste reduction strategies. The study also identifies seven types of plastic, each associated with different sources of accumulation or settlement. This variety presents both challenges and opportunities for waste management and recycling. Significantly, the research underscores the potential of repurposing plastic waste into recycled products, aligning with the circular economy model. This approach not only extends the lifecycle of plastic products but also contributes to reducing plastic waste generation and minimizing the environmental impact. Overall, the findings highlight the urgent need for improved waste management practices in Vietnam, particularly in urban and riverside areas, and advocate for innovative recycling solutions to mitigate the environmental challenges posed by plastic pollution.