Sixteen borate glass compositions, modified with K2O and SrO, were established using a design of mixtures approach and were subjected to chemical and physical characterization (11B MAS NMR, density, and glass transition temperature (Tg)). The analysis intended to establish the influence of K2O and SrO on the composition-structure-property relationships for borate glass networks. The effects of γ-radiation on the glass networks were also examined to determine the impact that sterilization may have on the glass properties. Structural analysis showed that both K2O and SrO influenced the fraction of three- and four-fold coordinated boron groups independent of the cation. Thermal analysis of the glasses revealed that SrO significantly increases the glass transition temperature while both SrO and K2O have comparable impacts on glass stability. These data suggest that Sr2+ cations cross-link the networks. Contrastingly, K+ appears to stabilize B4 structures. All sixteen compositions retained their chemical and physical characteristics following sterilization.