Terminator Region Research Articles

Plasma sheath around sunlit moon: monotonic and non-monotonic structures

ABSTRACTAn analytical investigation of the sheath formation over sunlit moon under the influence of observed solar ultraviolet/extreme ultraviolet radiation and solar wind plasma has been presented. Poisson’s equation is coupled with the latitude-dependent Fermionic photoelectrons, non-Maxwellian solar wind electrons and cold ions to derive the electric potential, electric field, and population density profiles within the photoelectron sheath. We notice that the high-energy photons of the solar spectra in addition to Lyman- α, significantly contribute in determining the surface charge and subsequent sheath structure. A traditional Debye (Type C) sheath forms around the terminator due to dominant plasma electron accretion and marginal photoemission. While moving towards the equator, the photoelectron contribution increases, yielding monotonic (Type B) and non-monotonic (Type A) sheath solutions over sunlit locations. The calculations show that the non-monotonic potential structures are more stable near the terminator region, while both types of potential structures are probable near the equator region for the nominal solar wind plasma. A vertical sheath extension of ∼60 m and ∼12 m is predicted for typical solar wind plasma at the equator corresponding to Type A and Type B sheath, respectively. Under exotic plasma conditions (e.g. Earth’s magnetospheric tail lobe, plasma sheet), non-monotonic solutions become stable for the photoemission-dominated region, and the entire sunlit lunar surface may acquire negative potential.

The landscape of Chlamydomonas histone H3 lysine 4 methylation reveals both constant features and dynamic changes during the diurnal cycle.

Chromatin modifications are epigenetic regulatory features with major roles in various cellular events, yet they remain understudied in algae. We interrogated the genome-wide distribution pattern of mono- and trimethylated histone H3 lysine 4 (H3K4) using chromatin-immunoprecipitation followed by deep-sequencing (ChIP-seq) during key phases of the Chlamydomonas cell cycle: early G1 phase, Zeitgeber Time 1 (ZT1), when cells initiate biomass accumulation, S/M phase (ZT13) when cells are replicating DNA and undergoing mitosis, and late G0 phase (ZT23) when they are quiescent. Tri-methylated H3K4 was predominantly enriched at transcription start sites of the majority of protein coding genes (85%). The likelihood of a gene being marked by H3K4me3 correlated with it being transcribed at some point during the life cycle but not necessarily by continuous active transcription, as exemplified by early zygotic genes, which may remain transcriptionally dormant for thousands of generations between sexual cycles. The exceptions to this rule were around 120 loci, some of which encode non-poly-adenylated transcripts, such as small nuclear RNAs and replication-dependent histones that had H3K4me3 peaks only when they were being transcribed. Mono-methylated H3K4 was the default state for the vast majority of histones that were bound outside of transcription start sites and terminator regions of genes. A small fraction of the genome that was depleted of any H3 lysine 4 methylation was enriched for DNA cytosine methylation and the genes within these DNA methylation islands were poorly expressed. Besides marking protein coding genes, H3K4me3 ChIP-seq data served also as a annotation tool for validation of hundreds of long non-coding RNA genes.

Aura-3D: A Three-dimensional Atmospheric Retrieval Framework for Exoplanet Transmission Spectra

Atmospheric retrievals of exoplanet transmission spectra allow constraints on the composition and structure of the day–night terminator region. Such retrievals in the past have typically assumed one-dimensional (1D) temperature structures which were adequate to explain extant observations. However, the increasing data quality expected from exoplanet spectroscopy with the James Webb Space Telescope (JWST) motivates considerations of multidimensional atmospheric retrievals. We present Aura-3D, a three-dimensional atmospheric retrieval framework for exoplanet transmission spectra. Aura-3D includes a forward model that enables rapid computation of transmission spectra in 3D geometry for a given atmospheric structure and can, therefore, be used for atmospheric retrievals as well as for computing spectra from general circulation models (GCMs). In order to efficiently explore the space of possible 3D temperature structures in retrievals, we develop a parametric 3D pressure–temperature profile which can accurately represent azimuthally averaged temperature structures of a range of hot Jupiter GCMs. We apply our retrieval framework to simulated JWST observations of hot Jupiter transmission spectra, obtaining accurate estimates of the day–night temperature variation across the terminator as well as the abundances of chemical species. We demonstrate an example of a model hot Jupiter transmission spectrum for which a traditional 1D retrieval of JWST-quality data returns biased abundance estimates, whereas a retrieval including a day–night temperature gradient can accurately retrieve the true abundances. Our forward model also has the capability to include inhomogeneous chemistry as well as variable clouds/hazes. This new retrieval framework opens the field to detailed multidimensional atmospheric characterization using transmission spectra of exoplanets in the JWST era.

Optimized Hemolysin Type 1 Secretion System in Escherichia coli by Directed Evolution of the Hly Enhancer Fragment and Including a Terminator Region.

Type 1 secretion systems (T1SS) have a relatively simple architecture compared to other classes of secretion systems and therefore, are attractive to be optimized by protein engineering. Here, we report a KnowVolution campaign for the hemolysin (Hly) enhancer fragment, an untranslated region upstream of the hlyA gene, of the hemolysin T1SS of Escherichia coli to enhance its secretion efficiency. The best performing variant of the Hly enhancer fragment contained five nucleotide mutations at five positions (A30U, A36U, A54G, A81U, and A116U) resulted in a 2‐fold increase in the secretion level of a model lipase fused to the secretion carrier HlyA1. Computational analysis suggested that altered affinity to the generated enhancer fragment towards the S1 ribosomal protein contributes to the enhanced secretion levels. Furthermore, we demonstrate that involving a native terminator region along with the generated Hly enhancer fragment increased the secretion levels of the Hly system up to 5‐fold.

Toward a multidimensional analysis of transmission spectroscopy

Considering the relatively high precision that will be reached by future observatories, it has recently become clear that one dimensional (1D) atmospheric models, in which the atmospheric temperature and composition of a planet are considered to vary only in the vertical, will be unable to represent exoplanetary transmission spectra with a sufficient accuracy. This is particularly true for warm to (ultra-) hot exoplanets because the atmosphere is unable to redistribute all the energy deposited on the dayside, creating a strong thermal and often compositional dichotomy on the planet. This situation is exacerbated by transmission spectroscopy, which probes the terminator region. This is the most heterogeneous region of the atmosphere. However, if being able to compute transmission spectra from 3D atmospheric structures (from a global climate model, e.g.) is necessary to predict realistic observables, it is too computationally expensive to be used in a data inversion framework. For this reason, there is a need for a medium-complexity 2D approach that captures the most salient features of the 3D model in a sufficiently fast implementation. With this in mind, we present a new open-source documented version of Pytmosph3R that handles the computation of transmission spectra for atmospheres with up to three spatial dimensions and can account for time variability. Taking the example of an ultrahot Jupiter, we illustrate how the changing orientation of the planet during the transit can allow us to probe the horizontal variations in the atmosphere. We further implement our algorithm in TauREx to allow the community to perform 2D retrievals. We describe our extensive cross-validation benchmarks and discuss the accuracy and numerical performance of each model.

Evidence for stellar contamination in the transmission spectra of HAT-P-12b

Context. Transmission spectroscopy characterizes the wavelength dependence of transit depth, revealing atmospheric absorption features in planetary terminator regions. In this context, different optical transmission spectra of HAT-P-12b reported in previous studies exhibited discrepant atmospheric features (e.g., Rayleigh scattering and alkali absorption). Aims. We aim to understand the atmosphere of HAT-P-12b using two transit spectroscopic observations by the Gran Telescopio Canarias (GTC) and to search for evidence of stellar activity contaminating the transmission spectra, which might be the reason behind the discrepancies. Methods. We used Gaussian processes to account for systematic noise in the transit light curves and used nested sampling for Bayesian inferences. We performed joint atmospheric retrievals using the two transmission spectra obtained by GTC OSIRIS, as well as previously published results, coupled with stellar contamination corrections for different observations. Results. The retrieved atmospheric model exhibits no alkali absorption signatures, but shows tentative molecular absorption features including H2O, CH4, and NH3. The joint retrieval of the combined additional public data analysis retrieves similar results, but with a higher metallicity. Conclusions. Based on Bayesian model comparison, the discrepancies of the transmission spectra of HAT-P-12b can be explained by the effect of different levels of unocculted stellar spots and faculae. In addition, we did not find strong evidence for a cloudy or hazy atmosphere from the joint analysis, which is inconsistent with prior studies based on the observations of the Hubble Space Telescope.

Constraining Low‐Altitude Lunar Dust Using the LADEE‐UVS Data

AbstractStudying lunar dust is vital to the exploration of the Moon and other airless planetary bodies. The Ultraviolet and Visible Spectrometer on board the Lunar Atmosphere and Dust Environment Explorer spacecraft conducted a series of Almost Limb activities to look for dust near the dawn terminator region. During these activities the instrument stared at a fixed point in the zodiacal background off the Moon's limb while the spacecraft moved in retrograde orbit from the sunlit to the unlit side of the Moon. The spectra obtained from these activities probe altitudes within a few kilometers of the Moon's surface, a region whose dust populations were not well constrained by previous remote‐sensing observations from orbiting spacecraft. Filtering these spectra to remove a varying instrumental signal enables constraints to be placed on potential signals from a dust atmosphere. These filtered spectra are compared with those predicted for dust atmospheres with various exponential scale heights and particle size distributions to yield upper limits on the dust number density for these potential populations. For a differential size distribution proportional to s−3 (where s is the particle size) and a scale height of 1 km, we obtain an upper limit on the number density of dust particles at the Moon's surface of 142 m−3.

Cloning and sequencing of the promoter and terminator regions of the rbcL gene from Gunnera perpensa L.

Primers were designed based on the chloroplast genome sequence from the cotton Gossypium barbadense L. Specificrestriction sites were included in the primers so as to facilitate the process of vector assembly for future chloroplast transformation. Polymerase chain reaction (PCR) was applied to amplify the promoter and terminator regions of the rbcLgene from the chloroplast DNA (cpDNA) of G. perpensa. These promoter and terminator regions of the rbcL gene were around 0.25 and 0.5 kilobase pair (kb) in length, respectively. The amplicons were purified and cloned into pGEM®-T Easy Vector System I, then transformed into DH5α strain of E. coli bacteria. Transformed bacteria were propagated and recombinant plasmids were extracted and sequenced. The resulted sequences were aligned and compared with the sequenced promoter and terminator of rbcL from cotton as the reference plant. The promoter region of rbcL gene from G. perpensa showed 81.5% identity with that of the cotton plant, while the terminator region of rbcL gene expressed 69.9% identity with that of cotton plant. The core sequences at -35 and -10 of the promoter from G. perpensa scored 54.44% of thedefault core sequences

Distribution of charge on floating dust particles over sunlit locations on Moon

Abstract The charging of fine particles within the photoelectron cloud is the key in characterizing the dusty plasma environment over sunlit locations. In this work, based on the statistical mechanics and charging kinetics of the floating dust particles within the photoelectron sheath over the sunlit lunar surface, the distribution of charge over floating particles has been investigated. The natural randomization of the dust charging currents, the anisotropy in sheath photoelectron population and electric field/ potential, and half Fermi Dirac velocity distribution of the emitted photoelectrons have been consistently accounted for in the formulation. In this framework, due to natural fluctuation, the charge on the floating dust particles is shown to be distributed over a wide range (∼100 e). This random fluctuation in charge might alter the static equilibrium of the floating dust particles and induce an altitudinal fluctuation (∼10%) about its mean position within the sheath. The concept and results suggest that the natural fluctuation of the dust charge could be a reason that the strict dust levitation under static force equilibrium is less likely to exhibit over the sunlit lunar surfaces. This effect of dust charge and subsequent altitudinal fluctuations have been found pronounced at larger latitudes (near the terminator region) and higher lunar altitudes.

Photoelectron Sheath and Plasma Charging on the Lunar Surface: Semianalytic Solutions and Fully-Kinetic Particle-in-Cell Simulations

This article presents the derivation of semianalytic solutions to a new 1-D photoelectron sheath model near the lunar surface. The plasma species include the cold solar wind protons, drifting Maxwellian solar wind electrons, and Maxwellian photoelectrons emitted from the surface. The semianalytic model is then numerically solved to obtain profiles of quantities of interest as functions of the vertical distance from the surface. A fully-kinetic 3-D finite-difference (FD) particle-in-cell (PIC) code is then utilized to simulate the 1-D photoelectron sheath and the results agree well with the numerical solution to the semianalytic model. A <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\kappa $ </tex-math></inline-formula> -distribution for solar wind electrons is also implemented to the FD-PIC code to compare with the Maxwellian distribution. Results show that photoelectron sheath may reach as high as close to 100 m above the illuminated flat lunar surface near the terminator region and up to about 50 m near the equator region. Our results show that under average solar wind condition, the photoelectron sheath profiles obtained with Maxwellian and <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\kappa $ </tex-math></inline-formula> -distribution (with <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\kappa = 4.5$ </tex-math></inline-formula> ) are very close for 1-D numerical results.

Constraining Mornings and Evenings on Distant Worlds: A new Semianalytical Approach and Prospects with Transmission Spectroscopy

Abstract The technique of transmission spectroscopy—the variation of a planetary radius with wavelength due to opacity sources in the planet’s terminator region—has been one of the most successful in the characterization of exoplanet atmospheres to date, providing key insights into the composition and structure of these distant worlds. A common assumption made when using this technique, however, is that the variations are the same in the entire terminator region. In reality, the morning and evening terminators might have distinct temperature, pressure, and thus compositional profiles due to the inherent 3D nature of the planet, which would, in turn, give rise to different spectra on each side of it. Constraining those might be fundamental for our understanding of not only the weather patterns in these distant worlds but also the planetary formation signatures that might only be possible to extract once these features are well understood. Motivated by this physical picture, in this work, we perform a detailed study of the observational prospects of detecting this effect. We present an open-source semianalytical framework with which this information can be extracted directly from transit light curves and perform a detailed study of the prospects of detecting the effect with current missions, such as TESS, and upcoming ones, such as JWST. Our results show that these missions show great promise for the detection of this effect. Transmission spectroscopy studies with JWST, in particular, could provide spectra of each of the limbs, allowing us to convey 3D information previously accessible only via phase curves.

Stable transfection system for Babesia sp. Xinjiang

BackgroundStable transfection systems have been described in many protozoan parasites, including Plasmodium falciparum, Cryptosporidium parvum, Babesia bovis, Babesia ovata, and Babesia gibsoni. For Babesia sp. Xinjiang (Bxj), which is the causative pathogen of ovine babesiosis and mainly prevails across China, the platform of those techniques remains absent. Genetic manipulation techniques are powerful tools to enhance our knowledge on parasite biology, which may provide potential drug targets and diagnostic markers.MethodsWe evaluated the inhibition efficiency of blasticidin (BSD) and WR99210 to Bxj. Then, a plasmid was constructed bearing selectable marker BSD, green fluorescent protein (GFP) gene, and rhoptry-associated protein-1 3′ terminator region (rap 3′ TR). The plasmid was integrated into the elongation factor-1 alpha (ef-1α) site of Bxj genome by cross-over homologous recombination technique. Twenty μg of plasmid was transfected into Bxj merozoites. Subsequently, drug selection was performed 24 h after transfection to generate transfected parasites.ResultsTransfected parasite lines, Bxj-c1, Bxj-c2, and Bxj-c3, were successfully obtained after transfection, drug selection, and colonization. Exogenous genes were integrated into the Bxj genome, which were confirmed by PCR amplification and sequencing. In addition, results of western blot (WB) and indirect immunofluorescence assay (IFA) revealed that GFP-BSD had expressed for 11 months.ConclusionsIn our present study, stable transfection system for Bxj was successfully developed. We anticipate that this platform will greatly facilitate basic research of Bxj.Graphical abstract

Investigating The GMO Existence in Chips and Breakfast Cereals Marketed in Turkey

In this research, processed or low processed samples containing corn or corn products (corn semolina, flour, etc.) and soybean were randomly collected from the market, and 25 products in total (chips, nuts, cereals, flour) were analyzed for genetic modification using DNA based detection method, the polymerase chain reaction. First, homogenization of the samples was performed. Then DNA isolation was done by using Cetyl Trimethyl Ammonium Bromide (CTAB) and Roche High Pure DNA Isolation Kit. Since the Roche High Pure DNA Isolation Kit gave better results, the analysis was completed with this method. After DNA isolation, the detection of the Lectin gene, Zein gene, CaMV 35S Promoter and NOS Terminator regions was performed by conventional PCR. Zein gene determination was done for searching and proving corn presence and similarly, Lectin gene determination was done for searching and proving soybean presence in the samples by conventional PCR. GMO3/GMO4 and Zein3/Zein4 primer pairs were used for Lectin and Zein gene determination, respectively. The amplification of DNA was observed in agarose gel electrophoresis. Lectin or Zein genes were detected in 17 samples while these genes were not detected in 8 samples. Samples, in which Lectin or Zein gene was detected were scanned for 35S promoter or NOS terminator. 35S-3/35S-6 and tNOS2F/tNOS2R primer pairs were used for scanning 35S Promoter and NOS Terminator, respectively. To observe possible contamination in the mix sterilized deionized water was used and 0% Bt-11 and 0% GTS 40-3-2 were used as a negative control, 5% Bt-11 and 10% GTS-40-3-2 were used as a positive control. All of the 25 samples did not provide enough DNA with the required quality. This result was considered to be sourced by the applications (frying, extruding, pressing etc.) that samples had been exposed to during processes. Neither 35S Promotor nor NOS Terminator was determined from any of the samples.

Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.

An evolutionarily conserved feature of introns is their ability to enhance expression of genes that harbor them. Introns have been shown to regulate gene expression at the transcription and post-transcription level. The general perception is that a promoter-proximal intron is most efficient in enhancing gene expression and the effect diminishes with the increase in distance from the promoter. Here we show that the intron regains its positive influence on gene expression when in proximity to the terminator. We inserted ACT1 intron into different positions within IMD4 and INO1 genes. Transcription Run-On (TRO) analysis revealed that the transcription of both IMD4 and INO1 was maximal in constructs with a promoter-proximal intron and decreased with the increase in distance of the intron from the promoter. However, activation was partially restored when the intron was placed close to the terminator. We previously demonstrated that the promoter-proximal intron stimulates transcription by affecting promoter directionality through gene looping-mediated recruitment of termination factors in the vicinity of the promoter region. Here we show that the terminator-proximal intron also enhances promoter directionality and results in compact gene architecture with the promoter and terminator regions in close physical proximity. Furthermore, we show that both the promoter and terminator-proximal introns facilitate assembly or stabilization of the preinitiation complex (PIC) on the promoter. On the basis of these findings, we propose that proximity to both the promoter and the terminator regions affects the transcription regulatory potential of an intron, and the terminator-proximal intron enhances transcription by affecting both the assembly of preinitiation complex and promoter directionality.

Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase

Theileria equi, an intraerythrocytic protozoan parasite, causes equine piroplasmosis, a disease which negatively impacts the global horse industry. Genetic manipulation is one of the research tools under development as a control method for protozoan parasites, but this technique needs to be established for T. equi. Herein, we report on the first development of a stable transgenic T. equi line expressing enhanced green fluorescent protein/blasticidin S deaminase (eGFP/BSD). To express the exogenous fusion gene in T. equi, regulatory regions of the elongation factor-1 alpha (ef-1α) gene were identified in T. equi. An eGFP/BSD-expression cassette containing the ef-1α gene promoter and terminator regions was constructed and integrated into the T. equi genome. On day 9 post-transfection, blasticidin-resistant T. equi emerged. In the clonal line of T. equi obtained by limiting dilution, integration of the eGFP/BSD-expression cassette was confirmed in the designated B-locus of the ef-1α gene via PCR and Southern blot analyses. Parasitaemia dynamics between the transgenic and parental T. equi lines were comparable in vitro. The eGFP/BSD-expressing transgenic T. equi and the methodology used to generate it offer new opportunities for better understanding of T. equi biology, with the add-on possibility of discovering effective control methods against equine piroplasmosis.

Catwoman: A transit modelling Python package for asymmetric light curves

When exoplanets pass in front of their stars, they imprint a transit signature on the stellar light curve which to date has been assumed to be symmetric in time, owing to the planet being modelled as a circular area occulting the stellar surface. However, this signature might be asymmetric due to different temperature/pressure and/or chemical compositions in the different terminator regions of the transiting planet. catwoman is a Python package that allows to model these asymmetric transit lightcurves, calculating lightcurves for any radially symmetric stellar limb darkening law, and where planets are modelled as two semi-circles, of different radii, using the integration algorithm developed in arXiv:1507.08285 and implemented in the batman library, from which catwoman builds upon.

Three-dimensional propagation of ion-acoustic waves in the plasma environment of the Venusian ionosphere

The propagation of electrostatic ion acoustic waves (IAWs) in a homogeneous, unmagnetized, and collisionless plasma system composed of two positive ions and superthermally distributed electrons is investigated. The three dimensional Kadomtsev-Petviashvili (KP) equation has been derived using the reductive perturbation analysis to describe the dynamics of the weakly nonlinear behavior of electrostatic IAWs in the Venusian ionosphere near the terminator region. A symbolic computational Painlevé analysis is employed to get various solutions of the KP equation. The stability of the localized structures solution against the variation in the transverse direction has been discussed. The condition for the localized pulses existence in the presence of transverse perturbations is examined via the pseudo-potential approach. Numerical studies to elaborate on the physical plasma parameters’ effects on the structural characteristics of nonlinear cnoidal, periodic, and soliton waves are graphically displayed. The model predicts a compressive supersonic IAWs can propagate in the Venusian ionosphere in agreement with the observations.

Unveiling cloudy exoplanets: the influence of cloud model choices on retrieval solutions

ABSTRACT In recent years, it has become clear that a substantial fraction of transiting exoplanets have some form of aerosol present in their atmospheres. Transit spectroscopy – mostly of hot Jupiters, but also of some smaller planets – has provided evidence for this, in the form of steep downward slopes from blue to red in the optical part of the spectrum, and muted gas absorption features throughout. Retrieval studies seeking to constrain the composition of exoplanet atmospheres must therefore account for the presence of aerosols. However, clouds and hazes are complex physical phenomena, and the transit spectra that are currently available allow us to constrain only some of their properties. Therefore, representation of aerosols in retrieval models requires that they are described by only a few parameters, and this has been done in a variety of ways within the literature. Here, I investigate a range of parametrizations for exoplanet aerosol and their effects on retrievals from transmission spectra of hot Jupiters HD 189733b and HD 209458b. I find that results qualitatively agree for the cloud/haze itself regardless of the parametrization used, and indeed using multiple approaches provides a more holistic picture; the retrieved abundance of H2O is also very robust to assumptions about aerosols. I also find strong evidence that aerosol on HD 209458b covers less than half of the terminator region, whilst the picture is less clear for HD 189733b.

Transcriptional control of gene expression in Pichia pastoris by manipulation of terminators.

Controlling gene expression is often the foremost goal of most biological endeavors like the production of industrial enzymes and expression of heterologous metabolic pathway genes. The components of the entire "expression cassette" exert control on net protein output. This control is primarily achieved through altering the promoter driving expression and by changing the copy number of the gene. However, there are only a few recent studies on terminators. Terminators are essential components in expression cassettes that influence the 3' end processing of mRNA, mRNA stability, and translational efficiency, which can modulate protein production. In Pichia pastoris (Komagataella phaffi), little attention has been paid to the selection of terminator regions in efforts to increase heterologous gene expression. To explore the potential application of the terminator regions on increased secretory production of Candida antarctica lipase B (CALB), we assessed the ability of three different classes of terminator regions: (1) terminator regions of methanol oxidation pathway genes of P. pastoris; (2) terminator regions of well-expressed and housekeeping genes of P. pastoris; and (3) terminators of other yeast genes like Saccharomyces cerevisiae. The terminator of dihydroxyacetone synthase (DHAS TT), a high expressing gene in the methanol utilization pathway, shows inducible CALB expression levels similar to the AOX1 terminator (AOX1 TT) under the control of AOX1 promoter and threefold higher in constitutive expression of CALB under the control of GAP promoter. The Calb transcript abundance was also found to correlate with protein expression. Furthermore, mRNA half-life determination showed a direct correlation between the stability of transcripts and increased transcription rate. Together, our results emphasize that enhancing transcript stability using the correct choice of transcription terminators (TT) will help in developing robust production strains suitable for scale-up.Key points• Influence of transcription terminators on Calb gene expression• Modulation of gene expression by enhancing transcript stability.

Origins of pits and troughs and degradation on a small primitive planetesimal in the Kuiper Belt: high-resolution topography of (486958) Arrokoth (aka 2014 MU69) from New Horizons

The dominant topographic features on two-lobed Kuiper Belt object (486958) Arrokoth (provisionally designated 2014 MU69) are scattered, small, circular depressions or pits up to ~1.0 km across and curvilinear troughs observed near the terminator during the New Horizons encounter of 01 January 2019. With important exceptions, evidence for an endogenic origin for pits is lacking and impact remains the most likely origin. Pit depths relative to the local surface are shallower than hypervelocity simple craters on icy moons and consistent with low velocity (~300 m/s) impacts in an icy target (the role of porosity and cohesion in cratering on Arrokoth being uncertain). There is, however, a large scatter in observed d/D, with some pits too shallow to measure reliably. The range of preservation states observed for both pits and troughs is consistent with slow but persistent degradation on the surface of this (and perhaps other) small primitive planetesimal(s) in the Kuiper Belt by agents such as micrometeorite bombardment or volatile loss, indicating that degradation does indeed occur on such bodies. Arrokoth pits could also be related to circular depressions on comet 9P/Tempel (though comparisons of surface features on Arrokoth and comets are intriguing they are limited by resolution differences and the evolved state of cometary surfaces). Two of the curvilinear troughs occur along the terminator of the Large Lobe. One trough is a shallow depression devoid of resolvable structures, the other features several linear scarps a few 10s of meters high, suggesting that coherent failure can occur on the surfaces of small Kuiper Belt objects such as Arrokoth. Several of the deepest small pits are also clustered within this walled trough, similar to tectonically associated pit chains on other planets. These pits and an obliquely viewed linear chain of elongate depressions remain the best candidates for endogenic features on Arrokoth. Subtle undulations and linear features no more than a few 10's of meters in amplitude (and even a few candidate positive relief features) are also evident in the terminator regions, indicating that the long-term evolution of this body may have been complex.