We demonstrate experimentally the first range-resolved detection and identification of biological aerosols in the air by non-linear lidar. Ultra-short terawatt laser pulses are used to induce two-photon-excited fluorescence (2PEF) in riboflavin-containing particles at a remote location. We show that, in the case of amino acid detection, 2PEF-lidar should be more efficient than linear 1PEF-lidar beyond a typical distance of 2 km, because it takes advantage of the higher atmospheric transmission at the excitation wavelengths. 2PEF-lidar moreover allows size measurement by pump–probe schemes, and pulse shaping may improve the detection selectivity.