Temporary Relief Research Articles

Autologous Conditioned Serum in Knee Osteoarthritis: A Systematic Review of Current Clinical Evidence.

Knee osteoarthritis (OA) significantly impacts global health, causing pain, disability, and socioeconomic burden. Traditional treatments often provide only temporary relief and can have adverse effects. Autologous conditioned serum (ACS) therapy, which enriches a patient's own blood with growth factors and anti-inflammatory cytokines, has emerged as a promising approach to manage knee OA, potentially offering pain reduction, improved function, and tissue regeneration. Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, we searched databases such as PubMed, Web of Science, and Cochrane using terms like "Autologous Conditioned Serum" and "knee osteoarthritis." Clinical studies were selected based on their focus on ACS's efficacy in knee OA, assessing outcomes like pain relief, functional improvement, and adverse events. Eighteen studies met the inclusion criteria, including randomized controlled trials, observational studies, and comparative analyses. The review included a wide range of study designs and outcomes, highlighting ACS's efficacy in reducing pain and enhancing knee function as evidenced by various patient-reported outcome measures Visual Analog Scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Knee Injury and Osteoarthritis Outcome Score (KOOS), Knee Society Clinical Rating Score (KSCRS) with a follow-up of up to 11 years (range: 2-11 years). Comparative studies showed ACS to be as effective or superior to conventional treatments such as platelet-rich plasma, steroids, and hyaluronic acid, especially in cases of moderate synovitis. Minimal adverse effects such as peri-injection pain, rigidity, synovitis, transient sensation of redness/heat, and numbness in the knee/leg/toes were reported, underscoring ACS's safety. Some studies suggested ACS might also have disease-modifying effects, contributing to tissue repair and integrity. ACS therapy offers a promising alternative for knee OA management, demonstrating potential benefits in symptom alleviation, functional improvement, and safety. Indications of disease-modifying properties further highlight its therapeutic value. However, the need for standardized formulations and treatment protocols, long-term studies, and mechanistic understanding remain. Future research should focus on addressing these gaps to fully elucidate ACS's role in the treatment landscape of knee OA.

Global Impacts of Extended COVID-19 Lockdowns: Intersecting Perspectives on Environmental Quality, Food Security, Human Health, and Societal Dynamics

The COVID-19 pandemic, an unprecedented global health crisis, prompted widespread implementation of prolonged lockdown measures. This study presents a multifaceted analysis of the extensive impacts of these lockdowns on environmental quality, food security, human health, and societal dynamics. Using a mixed-methods approach, the research synthesizes data from various sources, including environmental assessments, public health records, food supply chain analyses, and social surveys, to construct a comprehensive picture of the pandemic's repercussions. In the environmental domain, our analysis reveals a dual nature of impacts. Reduced industrial activity and transportation led to temporary improvements in air quality and lower greenhouse gas emissions in several regions. Wildlife sightings in urban areas increased, indicating temporary relief for particular ecosystems. Conversely, the pandemic exacerbated challenges like increased medical waste generation and heightened pressure on natural resources due to shifts in consumption patterns. Concerning food security, the study identifies a complex interplay of disrupted supply chains, altered agricultural practices, and varying government policies that led to both short-term scarcities and long-term concerns about food sustainability. While essential for controlling the virus spread, the lockdowns inadvertently created food production and distribution bottlenecks, disproportionately affecting vulnerable communities. The impact on human health extends beyond the direct effects of the COVID-19 virus. Our findings highlight increased mental health issues, including anxiety and depression, mainly attributable to prolonged isolation and economic uncertainties. Additionally, the disruption of routine healthcare services emerged as a critical concern, affecting non-COVID health issues. Societal dynamics underwent significant transformations. The study explores shifts in work culture, with remote working becoming a new norm, and examines the pandemic's impact on education, revealing a swift transition to online learning platforms. These changes, while innovative, also widened the digital divide and raised concerns about the long-term implications for social inequality. The study's overarching theme is the interconnectivity of these domains. Environmental changes influence food security, affecting human health and societal structures. The pandemic's multidimensional impacts necessitate a holistic response, integrating environmental, health, social, and economic strategies. In conclusion, this comprehensive analysis underscores the need for adaptive, resilient, and sustainable systems to mitigate future global crises. The COVID-19 pandemic, while presenting significant challenges, also offers a unique opportunity to rethink and redesign our approach to global health, environmental stewardship, food security, and societal well-being.

Dialectical Behavior Therapy for Reducing Emotional Eating in Emerging Adult Women

Emerging adulthood is a transitional period from adolescence to adulthood characterized by instability in roles and relationships as individuals navigate the demands of higher education, employment, intimate relationships, and self-identity formation. This transition often triggers various negative emotions, such as anxiety, distress, and self-doubt, particularly in women, making them more susceptible to engaging in emotional eating as a means of seeking temporary comfort or distraction. Emotional eating is a mechanism for temporary emotional relief, but feelings of regret or guilt often follow it. Emotion regulation skills are crucial when experiencing negative emotions. This study aims to demonstrate the effectiveness of Dialectical Behavior Therapy (DBT), an intervention designed to enhance emotion regulation skills, in reducing the occurrence of emotional eating among emerging adult women. The study employed a single case experiment design with two 21-year-old female participants experiencing emotional eating. The Emotional Eating Scale (EES) and emotional eating self-recording were used as measurement tools. Each participant received DBT over 7 sessions, each lasting 60-90 minutes. The results indicated that DBT reduced the frequency of emotional eating in both participants, with the effect persisting up to 14 days post-intervention. These findings suggest that DBT is an effective intervention for reducing emotional eating in emerging adult women.

Quero usar corticosteroides numa articulação. O que considerar?

The use of intra-articular corticosteroids is frequent in joint pathology, particularly in osteoarthritis. Although it offers a temporary relief of pain, inflammation and function, their long-term safety and efficacy remain controversial. Particulate corticosteroids have a sustained release, while non-particulate corticosteroids have a faster onset of action but a shorter duration of effect. This narrative review highlights the importance of informed IAC selection based on pharmacokinetics, potential benefits, and cartilage risks.

Managing Exercise-Induced Bronchoconstriction: A Comprehensive Review of Strategies for Athletes to Maintain Fitness and Health

Exercise-induced bronchoconstriction (EIB) significantly impacts athletes by impairing their performance and overall health. Characterized by airway narrowing during or after physical activity, EIB affects the quality of training and competition. This study employs a qualitative descriptive research model based on a comprehensive literature review to evaluate EIB management strategies in athletes. We systematically collected data from trusted scientific databases—Scopus, Science Direct, and Google Scholar—using keywords such as "Exercise-Induced Asthma," "Athlete," and "Sport." Relevant articles were identified, and key terms related to EIB management were reviewed. The data, including study designs, research objectives, sample sizes, and findings, were compiled and analyzed to determine effective management approaches. Key findings indicate that accurate diagnosis using spirometry and bronchial provocation tests is essential. Pharmacological management primarily involves inhaled corticosteroids (ICS) to reduce inflammation and control EIB, while short-acting beta agonists (SABAs) offer only temporary relief. Non-pharmacological strategies include avoiding triggers, implementing proper warm-up routines, and using breathing techniques. Supervised aerobic exercise programs were shown to improve cardiovascular fitness without worsening EIB symptoms. The study also reviewed the impact of environmental factors, such as air pollution and post-COVID-19 conditions, on EIB. Recommendations include personalized asthma action plans, training for coaches on environmental triggers, and exercising in controlled conditions. This study highlights the need for a holistic approach combining pharmacological and non-pharmacological methods to manage EIB effectively and maintain athletic performance. Further research is warranted to develop tailored strategies for athletes with EIB.

Ultrasound-Guided Blockade of the Lateral Cutaneous Branch of the Iliohypogastric Nerve to Differentiate Neuropathy From Greater Trochanteric Pain Syndrome: Cadaver Study and Initial Case Series

ObjectiveNeuropathy of the lateral cutaneous branch of the iliohypogastric nerve (LCBIN) may represent a differential diagnosis for greater trochanteric pain syndrome (GTPS). Ultrasound-guided neural blockade of the LCBIN may lead to diagnosis of this neuropathy. The aim of this study was to evaluate the accuracy of ultrasound-guided nerve block in cadavers and to present a first clinical case series of patients with neuropathy of the LCBIN where the workup for GTPS remained unremarkable. Ultrasound-guided nerve block led to pain relief in these patients, indicating LCBIN neuropathy. MethodsFirst, ultrasound-guided injections at the LCBIN were performed bilaterally in 24 fresh, non-frozen, non-embalmed body donors. Accuracy and nerve localization were validated by anatomic dissection. Second, a clinical case series of nine patients with suspected GTPS who underwent ultrasound-guided diagnostic LCBIN blockade was retrospectively analyzed. ResultsUltrasound-guided injections at the LCBIN yielded 91.7% accuracy (95% confidence interval: 0.80–0.98). On the right side the nerve was found within a range of 3–14 cm from the anterior superior iliac spine, and within a range of 7–15 cm on the left side. This clinical case series indicates that ultrasound-guided blockade at the LCBIN provides temporary pain relief and indicates the presence of LCBIN neuropathy. ConclusionUltrasound has demonstrated high accuracy for localization and injection guidance in the examination of LCBIN. Ultrasound-guided injection of local anesthetics may help to identify LCBIN neuropathy as a differential diagnosis in patients with suspected GTPS.

Transcatheter stenting of superior vena cava to treat postoperative SVC syndrome in a child: a case report

BackgroundSuperior vena cava (SVC) obstruction leading to SVC syndrome is an uncommon but potential complication of cardiac surgeries that involve dissection and anastomosis around the great vein. We present a case of iatrogenic SVC obstruction that was initially treated with transcatheter balloon angioplasty, which provided temporary relief, and ultimately resolved by stenting the affected segment.Case presentationThe index case underwent total anomalous pulmonary venous connection (TAPVC) repair and presented 3 months after surgery with features of SVC obstruction. Initially, transcatheter balloon angioplasty was performed, providing relief from the obstruction; however, the condition recurred within one month. Finally, the patient was treated with percutaneous stenting of superior vena cava, through femoral venous route, using 8 mm × 30 mm balloon-expandable bare metal stent (Formula 418, Cook Medical, Bloomington, IN). Remarkable relief of obstruction was established with decrease in mean gradient across SVC–right atrium junction to 2 mm Hg (from 12 mm Hg before balloon angioplasty and 18 mm Hg before stenting).ConclusionPercutaneous treatment for iatrogenic SVC obstruction developing after cardiac surgery appears to be effective. Close monitoring is required in the postoperative period for early diagnosis and timely intervention.

Epidural steroid injection technique

An epidural steroid injection (ESI) is a minimally invasive, image guided procedure for the treatment of back pain. Pain originating in the lumbar spine is the most common referral for ESI but the entire spine may be targeted. ESI can provide temporary but meaningful relief for patients who may have failed conservative management with oral analgesics and physical therapy. ESI may provide analgesia and anti-inflammatory effects that allow more conservative measures like physical therapy to become more effective. ESI also serves as a bridge between conservative and surgical management, intervention for post-surgical pain, or an alternative for non-surgical candidates. This article reviews the technique for performing ESI in the cervical, thoracic, and lumbosacral spine.

Effect of N-Acetyl Cysteine in the Treatment of Dry Eye Disease

Background: Dry eye disease (DED) is a multifactorial disorder characterized by tear film instability, ocular surface inflammation, and symptoms of discomfort that significantly impact patients' quality of life. Current treatments often provide only temporary relief, necessitating the exploration of novel therapeutic agents such as N-acetyl cysteine (NAC) to target underlying pathophysiological mechanisms. Objective: To evaluate the efficacy of N-acetyl cysteine in improving tear film stability and alleviating symptoms in patients with dry eye disease. Methods: This quasi-experimental study was conducted at the Department of Ophthalmology, Pak-Emirates Military Hospital, Rawalpindi, from January 2022 to May 2024. Sixty-six patients (132 eyes) aged 18-65 years, diagnosed with bilateral DED for at least three months, were enrolled. Participants were alternately assigned to the intervention group, which received NAC eye drops (0.1% w/v) administered as one drop every six hours, or the control group, which received sterile saline artificial tears (0.9%) at the same frequency. Baseline demographic and clinical data were collected. Tear Film Break-Up Time (TBUT) and Schirmer’s test scores were measured at baseline, two weeks, one month, and three months post-treatment. Additionally, symptoms of burning, itching, and foreign body sensation were assessed using a four-point scale. Data were analyzed using SPSS version 25.0, with a p-value of ≤0.05 considered statistically significant. Results: The median age of participants was 34.50 years (IQR: 16.00), with 35 (53.0%) being male. Significant improvements were observed in the NAC group compared to the control group at one and three months post-treatment. TBUT increased from a median of 7.00 seconds (IQR: 5.00) at baseline to 15.50 seconds (IQR: 8.00) at three months (p<0.001). Schirmer’s test scores improved from a median of 5.00 mm (IQR: 2.00) at baseline to 11.00 mm (IQR: 5.00) at three months (p<0.001). The NAC group also experienced a significant reduction in foreign body sensation at three months (p=0.023). Conclusion: N-acetyl cysteine is an effective treatment for improving tear film stability and reducing symptoms associated with dry eye disease. Its use offers significant benefits in both objective clinical measures and patient-reported outcomes, making it a valuable therapeutic option for managing DED.

Acute and preventive medical treatment of cluster headache in Taiwan: A narrative review.

Cluster headache (CH) is a debilitating neurological disorder characterized by severe, unilateral pain and ipsilateral autonomic symptoms. Chronic CH is exceedingly rare in Taiwan, constituting approximately 1% of all CH cases. This narrative review provides an up-to-date overview of the acute and preventive treatment strategies for CH in Taiwan, focusing on currently available pharmacological options in the country. The treatment approach for CH in Taiwan involves a stepwise strategy. High-flow oxygen and triptan nasal sprays are the mainstays of acute treatment, providing rapid relief and good tolerability. Transitional treatments, such as oral steroids and suboccipital steroid injections, serve as a crucial bridge between acute and long-term preventive therapies, offering temporary relief while minimizing side effects through a carefully limited duration. For preventive treatment, verapamil is the first-line option, with lithium and topiramate being the second-line alternatives. Among the CGRP monoclonal antibodies, galcanezumab has demonstrated efficacy in the prevention of episodic CH. Preventive treatments are personalized to individual patients, starting with low doses and close monitoring for adverse effects. Neuromodulatory therapies, such as non-invasive vagus nerve stimulation, show promise for chronic and refractory CH but have limited availability in Taiwan. In conclusion, despite the availability of various acute and preventive treatment options, unmet needs in the management of CH in Taiwan remain. In particular, increased awareness and education among healthcare professionals to improve the diagnosis and management of CH in Taiwan should be implemented.

Nrf2-mediated ferroptosis inhibition: a novel approach for managing inflammatory diseases.

Inflammatory diseases, including psoriasis, atherosclerosis, rheumatoid arthritis, and ulcerative colitis, are characterized by persistent inflammation. Moreover, the existing treatments for inflammatory diseases only provide temporary relief by controlling symptoms, and treatments of unstable and expensive. Therefore, new therapeutic solutions are urgently needed to address the underlying causes or symptoms of inflammatory diseases. Inflammation frequently coincides with a high level of (reactive oxygen species) ROS activation, serving as a fundamental element in numerous physiological and pathological phenotypes that can result in serious harm to the organism. Given its pivotal role in inflammation, oxidative stress, and ferroptosis, ROS represents a focal node for investigating the (nuclear factor E2-related factor 2) Nrf2 pathway and ferroptosis, both of which are intricately linked to ROS. Ferroptosis is mainly triggered by oxidative stress and involves iron-dependent lipid peroxidation. The transcription factor Nrf2 targets several genes within the ferroptosis pathway. Recent studies have shown that Nrf2 plays a significant role in three key ferroptosis-related routes, including the synthesis and metabolism of glutathione/glutathione peroxidase 4, iron metabolism, and lipid processes. As a result, ferroptosis-related treatments for inflammatory diseases have attracted much attention. Moreover, drugs targeting Nrf2 can be used to manage inflammatory conditions. This review aimed to assess ferroptosis regulation mechanism and the role of Nrf2 in ferroptosis inhibition. Therefore, this review article may provide the basis for more research regarding the treatment of inflammatory diseases through Nrf2-inhibited ferroptosis.

Hyperelastic material models for simulating deformation of silicone ring pessaries

Pessaries are removable gynecological prosthetic devices that provide mechanical support for temporary or long-term symptom relief of pelvic floor disorders, such as pelvic organ prolapse and stress urinary incontinence. To date, limited mechanical tests have been performed on physical pessary designs to characterize their behaviour under load; however, custom pessary manufacturing is expensive and time consuming. As an alternative, finite element (FE) modeling can provide detailed numerical insight into the response of a pessary design under load but to date has seen limited application, with little data available for pessary silicone materials. This study aimed to identify hyperelastic material models for two silicone materials used in custom pessary cocoon moulded manufacturing towards FE analysis of ring with support (RWS) pessaries. It was hypothesized that hyperelastic material models could be identified to capture the force and deformation response of multiple RWS sizes under different boundary conditions and silicone materials (Shore 60A and 40A). To understand the material characteristics of pessary silicone, uniaxial tension and compression tests were performed then the experimental data was fit with Mooney-Rivlin (MR) material models. To ensure the material models characterize the pessary behaviour, data from mechanical tests representing the RWS pessary folding and modified 3-point bending were compared to FE recreations (FEBio) of the same tests with the MR materials applied to the pessaries. The FE model results demonstrated good agreement in the force-displacement response for the fold and 3-point bending models for different pessary sizes and silicone stiffnesses. This work demonstrates the hyperelastic material models’ efficacy and will enable future studies to improve biomechanical analysis of silicone pessary designs.

Ultrasound-guided Supraclavicular Brachial Plexus Block for Therapeutic Management of Postoperative Compressive Brachial Plexus Neuropathy: A Case Report.

Compressive neuropathy of the brachial plexus is a common issue following laparoscopic and robotic surgeries. A 71-year-old male, post-lumbar spinal surgery, presented with excruciating right upper extremity pain and paresthesias. A supraclavicular brachial plexus (SBP) block with bupivacaine provided significant pain relief, lasting 36 hours. Subsequent physical therapy led to gradual pain and weakness improvement in compressive neuropathy. The SBP block, facilitated by ultrasound guidance, is a safe procedure with few serious complications. It proves beneficial for managing postoperative compressive neuropathy, allowing patients to break pain cycles and participate in rehabilitation. The SBP block is an effective addition to the management of postoperative compressive neuropathy, given its ease, safety, and potency. Although regional anesthesia provides only temporary relief, patients can experience a break in debilitating pain cycles associated with compressive neuropathy.

Evaluation of the Impact of Physical Therapy on Patients with Macromastia Seeking Breast Reduction Surgery.

Macromastia significantly impairs women's quality of life, with treatments such as physical therapy (PT) often providing only temporary relief. Insurance routinely denies breast reduction surgery, despite little relief after conservative treatments. Research on the efficacy of PT for macromastia is limited. This study investigated the efficacy of PT for macromastia and identified patient factors associated with progression to surgery. We conducted a retrospective cohort study of patients with macromastia between 2017 and 2021. We collected data on presenting symptoms, attempted conservative treatments, PT duration, and whether surgery was performed. All patients were invited to participate in a survey regarding their symptoms, treatments, and responses to treatments. A multiple logistic regression was used to evaluate symptoms associated patients progressing to a breast reduction. Among the 327 patients identified with macromastia, 312 (95.41%) reported back pain and 272 (83.18%) attempted PT. Of the 72 (22.02%) patients who responded to the survey, 152 (46.48%) underwent subsequent surgery. The mean time from initial consultation to surgery was 283 days. Bra strap grooving was associated with progression to surgery (OR=1.90, CI=1.02-3.54). Mean patient-reported pain levels decreased after surgery compared to after PT (pre-PT=7.1, post-PT=7.1, post-surgery=3.1, P<0.001). Patients frequently undergo PT prior to breast reduction surgery despite no significant reduction in pain, while those who undergo surgery experience permanent relief and significantly reduced pain. Patients with bra strap grooving are more likely to progress to surgery. The requirement for PT as a prerequisite for surgery by insurance companies should be reevaluated.

Insights into India's temporary air pollution relief: A systematic review for green recovery amid and post-COVID-19

Insights into India's temporary air pollution relief: A systematic review for green recovery amid and post-COVID-19

Rosmarinic acid-grafted gelatin nanogels for efficient diquafosol delivery in dry eye disease therapy

Dry eye disease (DED) is a prevalent ocular disorder characterized by unstable tear film condition with loss of aqueous or mucin, excessive oxidative stress, and inflammation, leading to discomfort and potential damage to the ocular surface. Current DED therapies have shown restricted therapeutic effects such as frequent dosing and temporary relief with potential unwanted side effects, urgently necessitating the development of innovative efficient therapeutic approaches. Herein, we developed rosmarinic acid (RosA) conjugated gelatin nanogels loading diquafosol sodium (DQS), DRGNG, for simultaneous ROS-scavenging and mucin-secreting DED treatment. Mechanically, DRGNG suppressed the ROS production, reduced inflammatory factors, and prompted mucin secretion in vitro and in vivo. The whole transcriptome RNA sequencing in vitro further provided a detailed analysis of the upregulation of anti-oxidant, anti-inflammatory, and mucin-promotion pathways. Therapeutically, both in evaporative DED and aqueous deficient DED models, the dual-functional DRGNG could prolong the retention time at the ocular surface, efficiently suppress the oxidative stress response, reverse ocular surface morphology, and recover tear film homeostasis, thus alleviating the DED when the dosage is halved compared to the commercial Diquas®. Our findings contribute to developing innovative therapies for DED and offer insights into the broader applications of nanogels in ocular drug delivery and oxidative stress-related conditions.

Early onset Bouveret syndrome: a unique case in a young patient

BackgroundBouveret syndrome is a rare etiology of gastric outlet obstruction, presenting with clinical manifestations that resemble those of several gastric pathologies. Timely diagnosis is imperative to mitigate potentially fatal consequences associated with this condition.Case presentationThis case report involves a male patient, aged 35, who exhibited symptoms of intense abdominal discomfort, nausea, and vomiting over a 24-h period. The individual experienced identical symptoms of lesser intensity on three separate occasions throughout the preceding 4-month period, during which they resorted to analgesic medication for temporary relief. A contrast-enhanced computed tomography (CT) scan of the abdomen was performed, which identified the presence of the Rigler triad, characterized by pneumobilia, a cholecysto-antral fistula, and an ectopic gallstone lodged in the gastric antrum resulting in an obstruction. The patient’s diagnosis of Bouveret syndrome was determined based on the imaging findings. A surgical procedure known as cholecystectomy was conducted, which confirmed the existence of the sizable cholecystoduodenal fistula that was previously detected in the computed tomography (CT) scan. The surgical procedures that were conducted included a partial duodenectomy, a gastrojejunostomy, a cholecystectomy, and a common bile duct exploration with the removal of common bile duct stones. After examining the bile duct, a t-tube was inserted into the bile duct.ConclusionThis case demonstrates a distinctive occurrence of a condition in a young man, typically observed in elderly females. There is a lack of established treatment guidelines for this particular entity. The appropriate course of action depends on various factors, including the patient’s condition, CT scan results, medical history, presence of co-existing illnesses, and intraoperative circumstances. These considerations are taken into account when determining whether a single-step surgical strategy or a multi-step approach should be pursued in order to minimize complications in patients. In this case, due to favorable conditions, a single-step approach was taken that yielded promising results.

CRITICAL REVIEW ON MATRA BASTI IN ANIDRA- A REVIEW ARTICLE

We live in the 21st century, an era characterised by industrialisation and a fast- faced lifestyle. This rapid pace has disrupted healthy living, leading to various lifestyle disorders, including insomnia. Today, more patients with different sleep pattern abnormalities, particularly insomnia, are seeking outpatient treatment. Insomnia is defined as the subjective perception or complaint of inadequate or poor- quality sleep, caused by factors such as difficulty falling asleep, frequent awakenings during the night, trouble returning to sleep, waking up too early, or unrefreshing sleep. In Ayurveda, it is comparable to Anidra and Nidranasha, with individuals of Vata and Pitta constitutions being more prone to insomnia. Asvapna is one of the 80 Nanatmaja Vata diseases. Allopathic treatments offer temporary relief but come with significant side effects. Ayurveda provides an effective approach to treating insomnia through both internal and external medications. Panchakarma therapy aims to remove imbalanced Doshas quickly. Matra Basti treatment soothes the mind and improves sleep.

Chondroitin Sulfate and Hyaluronic Acid-Based PolyHIPE Scaffolds for Improved Osteogenesis and Chondrogenesis In Vitro.

Osteochondral damage, affecting the articular cartilage and the underlying subchondral bone, presents significant challenges in clinical treatment. Such defects, commonly seen in knee and ankle joints, vary from small localized lesions to larger defects. Current medical therapies encounter several challenges, such as donor shortages, drug side effects, high costs, and rejection problems, often resulting in only temporary relief. Highly porous emulsion-templated polymers (polyHIPEs) offer numerous potential benefits in the fabrication of scaffolds for tissue engineering and regenerative medicine. Polymeric scaffolds synthesized using a high internal phase emulsion (HIPE) technique, called PolyHIPEs, involve polymerizing a continuous phase surrounding a dispersed internal phase to form a solid, foam-like structure. A dense, porous design encourages cell ingrowth, nutrient delivery, and waste disposal from the scaffold, mimicking the cells' natural microenvironment. This study used hydroxyethyl methacrylate (HEMA) and acrylamide (AAM) polyHIPE scaffolds combined with extracellular matrix (ECM) components of the tissue, such as methacrylated hyaluronic acid (MHA) and methacrylated chondroitin sulfate (MCS), to prepare polyHIPE scaffolds. The mouse preosteoblast MC3T3-E1 cells and primary rat chondrocytes (harvested from male Wistar rats) were seeded on the scaffolds and cultured for 21 days to assess the osteogenesis and chondrogenesis in vitro. When compared to the AAM-MHA and AAM-MCS groups at day 21, scaffold groups HEMA-MHA and HEMA-MCS showed a significant rise in alkaline phosphatase (ALP) and calcium content. Chondrogenic markers such as glycosaminoglycan (GAG) and hydroxyproline were also assessed over a 21-day time point. On day 21, it was found that GAG and hydroxyproline production were considerably higher in the HEMA-MHA and HEMA-MCS scaffolds than in the AAM-MHA and AAM-MCS scaffolds. The overall studies showed that polyHIPE monolith scaffolds could favor cell adherence, survival ability, proliferation, differentiation, and ECM formation over 21 days. Thus, incorporating ECM components enhanced osteogenesis and chondrogenesis in vitro and can be further used as tissue repair models.

Pandemic effect on company’s sustainability debt policy: The case of Portugal

Amid the relentless grip of the COVID-19 pandemic, sustainability has emerged as a paramount concern across global economies. As businesses grapple with unprecedented challenges, the imperative for sustainable practices in corporate finance becomes increasingly evident. Throughout this crisis, companies have faced staggering financial strains, with diminished turnovers and escalating operational costs pushing many to the brink of collapse. In response, governments worldwide have provided vital support, albeit often insufficient, underscoring the necessity for sustainable mechanisms of intervention. Central to this discourse is an examination of how companies have adapted their financing policies amidst the pandemic’s tumult. Government-backed credit facilities have served as a critical lifeline for numerous businesses, emphasizing the need for sustainable financial instruments readily deployable in times of crisis. Concurrently, moratoriums on existing credit obligations have offered temporary relief, albeit with looming concerns regarding heightened corporate indebtedness. Moreover, the pandemic’s aftermath has witnessed a pronounced uptick in corporate borrowing, compounded by surging interest rates. This confluence underscores the exigency for companies to adopt sustainable financial strategies, mindful not only of short-term exigencies but also the enduring ramifications on financial stability. In navigating these challenges, a holistic approach to sustainability is imperative. Governments must ensure robust support mechanisms, while companies must proactively seek sustainable financing solutions. Concurrently, stakeholders must meticulously weigh the long-term repercussions of financial policy adjustments, thereby fortifying corporate resilience against future crises while safeguarding the stability of the global economy. In essence, the COVID-19 pandemic has underscored the critical imperative for sustainability in corporate finance. By heeding this call and embracing sustainable practices, businesses can navigate crises with greater resilience, ensuring not only their survival but also the enduring stability of the economic landscape.