Urban river pollution is considered a ‘necessary evil’ consequence of disproportionate developmental expansion in metropolises. Unprecedented expansion and anthropic activities lead to the deterioration of urban rivers with municipal and industrial sewage. The construction of sluices is one of the irrefutable parts of the process. In order to prevent floods and drought, many cities build sluices and dams in rivers to balance water quantity in different seasons. To explore the change characteristics of the water quality in urban rivers after the construction of sluices and dams, the change in the total phosphorus (TP) and total nitrogen (TN) concentrations upstream and downstream of rivers was investigated under the condition of sluices closure in Wuxi. According to the results, when the sluices were closed, the pollutants of TP and TN would accumulate upstream in rivers, which caused the water quality in the upper reaches to be worse than that in the lower reaches. Specifically, the TN and TP concentrations downstream of urban rivers in Wuxi were approximately 14.42% and 13.80% lower than those upstream when the sluices were closed. Additionally, the water quality in urban rivers was usually better in summer and autumn than in the other seasons, showing obvious seasonality after the construction of the sluices. The research will provide a theoretical basis for future sluice operation and the water resources management of urban rivers.